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Microfluidics has seen a remarkable growth over the past few decades, with its extensive applications in engineering, medicine, biology, chemistry, etc [...].
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This paper investigates the electroosmotic micromixing of non-Newtonian fluid in a microchannel with wall-mounted obstacles and surface potential heterogeneity on the obstacle surface. In the numerical simulation, the full model consisting of the Navier-Stokes equations and the Poisson-Nernst-Plank equations are solved for the electroosmotic fluid field, ion transport, and electric field, and the power law model is used to characterize the rheological behavior of the aqueous solution. The mixing performance is investigated under different parameters, such as electric double layer thickness, flow behavior index, obstacle surface zeta potential, obstacle dimension. Due to the zeta potential heterogeneity at the obstacle surface, vortical flow is formed near the obstacle surface, which can significantly improve the mixing efficiency. The results show that, the mixing efficiency can be improved by increasing the obstacle surface zeta potential, the flow behavior index, the obstacle height, the EDL thickness.
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: Electroosmotic flow (EOF) of viscoelastic fluid with Linear Phan-Thien-Tanner (LPTT) constitutive model in a nanochannel connecting two reservoirs is numerically studied. For the first time, the influence of viscoelasticity on the EOF and the ionic conductance in the micro-nanofluidic interconnect system, with consideration of the electrical double layers (EDLs), is investigated. Regardless of the bulk salt concentration, significant enhancement of the flow rate is observed for viscoelastic fluid compared to the Newtonian fluid, due to the shear thinning effect. An increase in the ionic conductance of the nanochannel occurs for the viscoelastic fluid. The enhancement of the ionic conductance is significant under the overlapping EDLs condition.
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The electroosmotic flow (EOF) of viscoelastic fluid in a long nanoslit is numerically studied to investigate the rheological property effect of Linear Phan-Thien-Tanner (LPTT) fluid on the fully developed EOF. The non-linear Poisson-Nernst-Planck equations governing the electric potential and the ionic concentration distribution within the channel are adopted to take into account the effect of the electrical double layer (EDL), including the EDL overlap. When the EDL is not overlapped, the velocity profiles for both Newtonian and viscoelastic fluids are plug-like and increase sharply near the charged wall. The velocity profile resembles that of pressure-driven flow when the EDL is overlapped. Regardless of the EDL thickness, apparent increase of velocity is obtained for viscoelastic fluid of larger Weissenberg number compared to the Newtonian fluid, indicating the shear thinning behavior of the LPTT fluid. The effect of the Weissenberg number on the velocity distribution is less significant as the degree of EDL overlapping increases, due to the overall decrease of the shear rate. The increase (decrease) of polymer extensibility (viscosity ratio) also enhances the EOF of viscoelastic fluid.
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Background: Idesia polycarpa Maxim. var. vestita Diels, a dioecious plant, is widely used for biodiesel due to the high oil content of its fruits. However, it is hard to distinguish its sex in the seedling stage, which makes breeding and production problematic as only the female tree can produce fruits, and the mechanisms underlying sex determination and differentiation remain unknown due to the lack of available genomic and transcriptomic information. To begin addressing this issue, we performed the transcriptome analysis of its female and male flower. Results: 28,668,977 and 22,227,992 clean reads were obtained from the female and male cDNA libraries, respectively. After quality checks and de novo assembly, a total of 84,213 unigenes with an average length of 1179 bp were generated and 65,972 unigenes (78.34%) could be matched in at least one of the NR, NT, Swiss-Prot, COG, KEGG and GO databases. Functional annotation of the unigenes uncovered diverse biological functions and processes, including reproduction and developmental process, which may play roles in sex determination and differentiation. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed many unigenes annotated as metabolic pathways, biosynthesis of secondary metabolites pathways, plant pathogen interaction, and plant hormone signal transduction. Moreover, 29,953 simple sequence repeats were identified using the microsatellite software. Conclusion: This work provides the first detailed transcriptome analysis of female and male flower of I. polycarpa and lays foundations for future studies on the molecular mechanisms underlying flower bud development of I. polycarpa.
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Reprodução/genética , Salicaceae/genética , Transcriptoma , Análise de Sequência de RNA , Genes de Plantas , Repetições de Microssatélites , Salicaceae/crescimento & desenvolvimento , Bases de Dados Genéticas , Sequenciamento de Nucleotídeos em Larga Escala , Anotação de Sequência MolecularRESUMO
Almost all the reported capillary convective polymerase chain reaction (CCPCR) systems to date are still limited to research use stemming from unresolved issues related to repeatability, reliability, convenience, and sensitivity. To move CCPCR technology forward toward commercialization, a couple of critical strategies and innovations are discussed here. First, single- and dual-end heating strategies are analyzed and compared between each other. Especially, different solutions for dual-end heating are proposed and discussed, and the heat transfer and fluid flow inside the capillary tube with an optimized dual-end heating strategy are analyzed and modeled. Second, real-time CCPCR is implemented with light-emitting diode and photodiode, and the real-time fluorescence detection method is compared with the post-amplification end-point detection method based on a dipstick assay. Thirdly, to reduce the system complexity, e.g., to simplify parameter tuning of the feedback control, an internal-model-control-based proportional-integral-derivative controller is adopted for accurate temperature control. Fourth, as a proof of concept, CCPCR with pre-loaded dry storage of reagent inside the capillary PCR tube is evaluated to better accommodate to point-of-care diagnosis. The critical performances of improved CCPCR, especially with sensitivity, repeatability, and reliability, have been thoroughly analyzed with different experiments using influenza A (H1N1) virus as the detection sample.
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Epithelial cells form quasi-two-dimensional sheets that function as contractile media to effect tissue shape changes during development and homeostasis. Endogenously generated intrasheet tension is a driver of such changes, but has predominantly been measured in the presence of directional migration. The nature of epithelial cell-generated forces transmitted over supracellular distances, in the absence of directional migration, is thus largely unclear. In this report, we consider large epithelial cell colonies which are archetypical multicell collectives with extensive cell-cell contacts but with a symmetric (circular) boundary. Using the traction force imbalance method (TFIM) (traction force microscopy combined with physical force balance), we first show that one can determine the colony-level endogenous sheet forces exerted at the midline by one half of the colony on the other half with no prior assumptions on the uniformity of the mechanical properties of the cell sheet. Importantly, we find that this colony-level sheet force exhibits large variations with orientation-the difference between the maximum and minimum sheet force is comparable to the average sheet force itself. Furthermore, the sheet force at the colony midline is largely tensile but the shear component exhibits significantly more variation with orientation. We thus show that even an unperturbed epithelial colony with a symmetric boundary shows significant directional variation in the endogenous sheet tension and shear forces that subsist at the colony level.
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Células Epiteliais/citologia , Fenômenos Mecânicos , Animais , Fenômenos Biomecânicos , Cães , Células Madin Darby de Rim Canino , Resistência ao Cisalhamento , Resistência à TraçãoRESUMO
MicroRNAs (miRNAs) play important roles in nearly every aspect of biology, including physiological, biochemical, developmental and pathological processes. Therefore, a highly sensitive and accurate method of detection of miRNAs has great potential in research on theory and application, such as the clinical approach to medicine, animal and plant production, as well as stress response. Here, we report a strategic method to detect miRNAs from multicellular organisms, which mainly includes liquid hybridization and solid phase detection (LHSPD); it has been verified in various species and is much more sensitive than traditional biotin-labeled Northern blots. By using this strategy and chemiluminescent detection with digoxigenin (DIG)-labeled or biotin-labeled oligonucleotide probes, as low as 0.01-0.25 fmol [for DIG-CDP Star (disodium2-chloro-5-(4-methoxyspiro{1,2-dioxetane-3,2'-(5'-chloro)tricyclo[3.3.1.13,7]decan}-4-yl)phenyl phosphate) system], 0.005-0.1 fmol (for biotin-CDP Star system), or 0.05-0.5 fmol (for biotin-luminol system) of miRNA can be detected and one-base difference can be distinguished between miRNA sequences. Moreover, LHSPD performed very well in the quantitative analysis of miRNAs, and the whole process can be completed within about 9 h. The strategy of LHSPD provides an effective solution for rapid, accurate, and sensitive detection and quantitative analysis of miRNAs in plants and animals.
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Northern Blotting/métodos , MicroRNAs/análise , Hibridização de Ácido Nucleico/métodos , Animais , Arabidopsis , Biotinilação/métodos , Drosophila melanogaster , Camundongos , MicroRNAs/química , Oryza , NicotianaRESUMO
Proton transport in confined spaces plays a crucial role in many biological processes as well as in modern technological applications, such as fuel cells. To achieve active control of proton conductance, we investigate for the first time the gate modulation of proton transport in a pH-regulated nanopore by a multi-ion model. The model takes into account surface protonation/deprotonation reactions, surface curvature, electroosmotic flow, Stern layer, and electric double layer overlap. The proposed model is validated by good agreement with the existing experimental data on nanopore conductance with and without a gate voltage. The results show that the modulation of proton transport in a nanopore depends on the concentration of the background salt and solution pH. Without background salt, the gated nanopore exhibits an interesting ambipolar conductance behavior when pH is close to the isoelectric point of the dielectric pore material, and the net ionic and proton conductance can be actively regulated with a gate voltage as low as 1 V. The higher the background salt concentration, the lower is the performance of the gate control on the proton transport.
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A multi-ion model taking into account the Stern layer effect and the surface chemistry reactions is developed for the first time to investigate the surface charge properties and electrophoresis of pH-regulated silica nanoparticles (NPs). The applicability of the model is validated by comparing its prediction to the experimental data of the electrophoretic mobility of silica NPs available from the literature. Results show that if the particle size is fixed, the Stern layer effect on the surface charge properties of the NP is notable at high pH and background salt concentration; however, that effect on the particle mobility is significant when pH is around neutrality and the salt concentration is medium high (ca. 0.07 M) because of the double-layer polarization effect. Moreover, if pH and the background salt concentration are fixed, the Stern layer effect on the zeta potential and electrophoretic mobility of the NP becomes more significant for smaller particle size. Neglecting the Stern layer effect could result in the overestimation of the zeta potential, surface charge density, and electrophoretic mobility of a NP on the order of several times.
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Accurately and rapidly analyzing the ionic current/conductance in a nanochannel, especially under the condition of overlapped electric double layers (EDLs), is of fundamental significance for the design and development of novel nanofluidic devices. To achieve this, an analytical model for the surface charge properties and ionic current/conductance in a pH-regulated nanochannel is developed for the first time. The developed model takes into account the effects of the EDL overlap, electroosmotic flow, Stern layer, multiple ionic species, and the site dissociation/association reactions on the channel walls. In addition to good agreement with the existing experimental data of nanochannel conductance available from the literature, our analytical model is also validated by the full model with the Poisson-Nernst-Planck and Navier-Stokes equations. The EDL overlap effect is significant at small nanochannel height, low salt concentration, and medium low pH. Neglecting the EDL overlap effect could result in a wrong estimation in the zeta potential and conductance of the nanochannel in a single measurement.