RESUMO
FPA is a gene that regulates flowering time in Arabidopsis via a pathway that is independent of daylength (the autonomous pathway). Mutations in FPA result in extremely delayed flowering. FPA was identified by means of positional cloning. The predicted FPA protein contains three RNA recognition motifs in the N-terminal region. FPA is expressed most strongly in developing tissues, similar to the expression of FCA and LUMINIDEPENDENS, two components of the autonomous pathway previously identified. Overexpression of FPA in Arabidopsis causes early flowering in noninductive short days and creates plants that exhibit a more day-neutral flowering behavior.
Assuntos
Proteínas de Arabidopsis , Arabidopsis/genética , Genes de Plantas , Proteínas de Plantas/genética , Proteínas de Ligação a RNA/genética , Alelos , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Arabidopsis/crescimento & desenvolvimento , Clonagem Molecular , Expressão Gênica , Humanos , Dados de Sequência Molecular , Proteínas de Plantas/metabolismo , Proteínas de Plantas/fisiologia , RNA de Plantas/metabolismo , Proteínas de Ligação a RNA/metabolismo , Proteínas de Ligação a RNA/fisiologia , Homologia de Sequência de AminoácidosRESUMO
Arabidopsis cyt1 mutants have a complex phenotype indicative of a severe defect in cell wall biogenesis. Mutant embryos arrest as wide, heart-shaped structures characterized by ectopic accumulation of callose and the occurrence of incomplete cell walls. Texture and thickness of the cell walls are irregular, and unesterified pectins show an abnormally diffuse distribution. To determine the molecular basis of these defects, we have cloned the CYT1 gene by a map-based approach and found that it encodes mannose-1-phosphate guanylyltransferase. A weak mutation in the same gene, called vtc1, has previously been identified on the basis of ozone sensitivity due to reduced levels of ascorbic acid. Mutant cyt1 embryos are deficient in N-glycosylation and have an altered composition of cell wall polysaccharides. Most notably, they show a 5-fold decrease in cellulose content. Characteristic aspects of the cyt1 phenotype, including radial swelling and accumulation of callose, can be mimicked with the inhibitor of N-glycosylation, tunicamycin. Our results suggest that N-glycosylation is required for cellulose biosynthesis and that a deficiency in this process can account for most phenotypic features of cyt1 embryos.
Assuntos
Arabidopsis/genética , Celulose/biossíntese , Genes de Plantas , Mutação , Nucleotidiltransferases/genética , Sequência de Aminoácidos , Arabidopsis/enzimologia , Arabidopsis/metabolismo , Northern Blotting , Western Blotting , Clonagem Molecular , Glicosilação , Dados de Sequência Molecular , Nucleotidiltransferases/metabolismo , Raízes de Plantas/metabolismo , Homologia de Sequência de AminoácidosRESUMO
Arabidopsis thaliana, a small annual plant belonging to the mustard family, is the subject of study by an estimated 7000 researchers around the world. In addition to the large body of genetic, physiological and biochemical data gathered for this plant, it will be the first higher plant genome to be completely sequenced, with completion expected at the end of the year 2000. The sequencing effort has been coordinated by an international collaboration, the Arabidopsis Genome Initiative (AGI). The rationale for intensive investigation of Arabidopsis is that it is an excellent model for higher plants. In order to maximize use of the knowledge gained about this plant, there is a need for a comprehensive database and information retrieval and analysis system that will provide user-friendly access to Arabidopsis information. This paper describes the initial steps we have taken toward realizing these goals in a project called The Arabidopsis Information Resource (TAIR) (www.arabidopsis.org).
Assuntos
Arabidopsis/genética , Bases de Dados Factuais , Mapeamento Cromossômico , Genoma de Planta , Serviços de Informação , Armazenamento e Recuperação da Informação , InternetRESUMO
We describe in this report a novel class of mutants that should facilitate the identification of genes required for progression through the mitotic cell cycle during seed development in angiosperms. Three non-allelic titan (ttn) mutants with related but distinct phenotypes are characterized. The common feature among these mutants is that endosperm nuclei become greatly enlarged and highly polyploid. The mutant embryo is composed of a few giant cells in ttn1, several small cells in ttn2, and produces a normal plant in ttn3. Condensed chromosomes arrested at prophase of mitosis are found in the free nuclear endosperm of ttn1 and ttn2 seeds. Large mitotic figures with excessive numbers of chromosomes are visible in ttn3 endosperm. The ttn1 mutation appears to disrupt cytoskeletal organization because endosperm nuclei fail to migrate to the chalazal end of the seed. How double fertilization leads to the establishment of distinct patterns of mitosis and cytokinesis in the embryo and endosperm is a central question in plant reproductive biology. Molecular isolation of TITAN genes should help to answer this question, as well as related issues concerning cell cycle regulation, chromosome movement and endosperm identity in angiosperms.
Assuntos
Arabidopsis/genética , Ciclo Celular/genética , Mitose/genética , Mutação , Sementes/crescimento & desenvolvimento , Arabidopsis/crescimento & desenvolvimento , Fenótipo , Sementes/genéticaRESUMO
Arabidopsis thaliana is a small plant in the mustard family that has become the model system of choice for research in plant biology. Significant advances in understanding plant growth and development have been made by focusing on the molecular genetics of this simple angiosperm. The 120-megabase genome of Arabidopsis is organized into five chromosomes and contains an estimated 20,000 genes. More than 30 megabases of annotated genomic sequence has already been deposited in GenBank by a consortium of laboratories in Europe, Japan, and the United States. The entire genome is scheduled to be sequenced by the end of the year 2000. Reaching this milestone should enhance the value of Arabidopsis as a model for plant biology and the analysis of complex organisms in general.
Assuntos
Arabidopsis/genética , Mapeamento Cromossômico , Genoma de Planta , Análise de Sequência de DNA , Arabidopsis/fisiologia , Biotecnologia , Bases de Dados Factuais , Genes de Plantas , Cooperação Internacional , Mutagênese , Homologia de Sequência do Ácido NucleicoRESUMO
The genetic control of cell division in eukaryotes has been addressed in part through the analysis of cytokinesis-defective mutants. Two allelic mutants of Arabidopsis (cyt1-1 and cyt1-2) altered in cytokinesis and cell-wall architecture during embryogenesis are described in this report. Mutant embryos appear slightly abnormal at the heart stage and then expand to form a somewhat disorganized mass of enlarged cells with occasional incomplete walls. In contrast to the keule and knolle mutants of Arabidopsis and the cyd mutant of pea, which also exhibit defects in cytokinesis during embryogenesis, cyt1 embryos cannot be rescued in culture, are desiccation-intolerant at maturity, and produce cell walls with excessive callose as revealed through staining with the aniline blue fluorochrome, Sirofluor. Some cyt1 defects can be partially phenocopied by treatment with the herbicide dichlobenil, which is thought to interfere with cellulose biosynthesis. The distribution of unesterified pectins in cyt1 cell walls is also disrupted as revealed through immunocytochemical localization of JIM 5 antibodies. These features indicate that CYT1 plays an essential and unique role in plant growth and development and the establishment of normal cell-wall architecture.
Assuntos
Arabidopsis/citologia , Arabidopsis/genética , Glucanos/metabolismo , Mutação , Alelos , Arabidopsis/metabolismo , Divisão Celular/genética , Parede Celular/metabolismo , Parede Celular/ultraestrutura , Microscopia Eletrônica , Modelos Biológicos , Pectinas/metabolismoRESUMO
Spontaneous twinning is a widespread but infrequent phenomenon in higher plants. We describe here a mutant of Arabidopsis thaliana, twin, that yields an unusually high frequency of viable twin and occasional triplet seedlings. Supernumerary embryos of twin arise through a novel mechanism: transformation of cells within the suspensor, a differentiated structure established early in embryogenesis. Twin embryos develop in tandem within the seed, connected by intact segments of the suspensor. Transformed suspensor cells appear to duplicate the patterns of cell division and developmental pathways characteristic of zygotic embryogenesis. In addition to polyembryony, mutant embryos exhibit a number of developmental defects, including irregular patterns of cell division and abnormal morphology. The TWIN locus therefore appears to be required for normal development of the embryo proper as well as suppression of embryogenic potential in the suspensor. The development of viable secondary embryos in twin demonstrates that cells of the Arabidopsis suspensor can successfully establish embryonic polarity and complete the full spectrum of developmental programs normally restricted to the embryo proper. In addition, the twin phenotype indicates that disruption of a single genetic locus can result in the conversion of a single terminally differentiated cell type to an embryogenic state.
Assuntos
Arabidopsis/embriologia , Divisão Celular , Mapeamento Cromossômico , Regulação da Expressão Gênica no Desenvolvimento , Genes de Plantas , Ligação Genética , Mutação , FenótipoRESUMO
We have previously described a homeotic leafy cotyledon (lec) mutant of Arabidopsis that exhibits striking defects in embryonic maturation and produces viviparous embryos with cotyledons that are partially transformed into leaves. In this study, we present further details on the developmental anatomy of mutant embryos, characterize their response to abscisic acid (ABA) in culture, describe other mutants with related phenotypes, and summarize studies with double mutants. Our results indicate that immature embryos precociously enter a germination pathway after the torpedo stage of development and then acquire characteristics normally restricted to vegetative parts of the plant. In contrast to other viviparous mutants of maize (vp1) and Arabidopsis (abi3) that produce ABA-insensitive embryos, immature lec embryos are sensitive to ABA in culture. ABA is therefore necessary but not sufficient for embryonic maturation in Arabidopsis. Three other mutants that produce trichomes on cotyledons following precocious germination in culture are described. One mutant is allelic to lec1, another is a fusca mutant (fus3), and the third defines a new locus (lec2). Mutant embryos differ in morphology, desiccation tolerance, pattern of anthocyanin accumulation, presence of storage materials, size and frequency of trichomes on cotyledons, and timing of precocious germination in culture. The leafy cotyledon phenotype has therefore allowed the identification of an important network of regulatory genes with overlapping functions during embryonic maturation in Arabidopsis.
RESUMO
Arabidopsis fusca mutants display striking purple coloration due to anthocyanin accumulation in their cotyledons. We describe six recessive fusca mutants isolated from Agrobacterium-transformed Arabidopsis families. These mutants first become defective during embryogenesis and exhibit limited seedling development. Double mutant constructs revealed that developmental defects were not simply a consequence of anthocyanin accumulation. fusca seedlings showed altered responses to several environmental and endogenous factors. Allelism tests established that three fusca loci are represented by mutants previously described as defective in light-regulated responses. To study the molecular basis of the fusca phenotype, we cloned the FUS6 gene. FUS6 encodes a novel protein that is hydrophilic, alpha-helical, and contains potential protein kinase C phosphorylation sites. The FUSCA proteins appear to act in a network of signal transduction pathways critical for plant development.
Assuntos
Proteínas de Arabidopsis , Arabidopsis/genética , Proteínas de Ligação ao GTP , Genes de Plantas , Proteínas de Plantas/genética , Proteínas , Proteínas Repressoras , Alelos , Sequência de Aminoácidos , Antocianinas/metabolismo , Arabidopsis/embriologia , Arabidopsis/metabolismo , Arabidopsis/efeitos da radiação , Sequência de Bases , Complexo do Signalossomo COP9 , Metabolismo dos Carboidratos , Clonagem Molecular , DNA , Peptídeos e Proteínas de Sinalização Intracelular , Luz , Dados de Sequência Molecular , Mutação , Fenótipo , Reguladores de Crescimento de Plantas/fisiologia , Proteínas de Plantas/metabolismo , Proteína Quinase C/metabolismo , Sementes/crescimento & desenvolvimento , Transdução de SinaisRESUMO
Over 5000 transgenic families of Arabidopsis thaliana produced following seed transformation with Agrobacterium tumefaciens were screened for embryonic lethals, defectives, and pattern mutants. One hundred and seventy-eight mutants with a wide range of developmental abnormalities were identified. Forty-one mutants appear from genetic studies to be tagged (36% of the 115 mutants examined in detail). Mapping with visible markers demonstrated that mutant genes were randomly distributed throughout the genome. Seven mutant families appeared to contain chromosomal translocations because the mutant genes exhibited linkage to visible markers on two different chromosomes. Chromosomal rearrangements may therefore be widespread following seed transformation. DNA gel blot hybridizations with 34 tagged mutants and three T-DNA probes revealed a wide range of insertion patterns. Models of T-DNA structure at each mutant locus were constructed to facilitate gene isolation. The value of such models was demonstrated by using plasmid rescue to clone flanking plant DNA from four tagged mutants. Further analysis of genes isolated from these insertional mutants should help to elucidate the relationship between gene function and plant embryogenesis.
Assuntos
Arabidopsis/embriologia , Arabidopsis/genética , Sequência de Bases , Mapeamento Cromossômico , Rearranjo Gênico , Dados de Sequência Molecular , Mutagênese Insercional , Fenótipo , Plantas Geneticamente Modificadas , Plasmídeos , Mapeamento por Restrição , Transformação GenéticaRESUMO
Cotyledons are specialized leaves produced during plant embryogenesis. Cotyledons and leaves typically differ in morphology, ultrastructure, and patterns of gene expression. The leafy cotyledon (Iec) mutant of Arabidopsis thaliana fails to maintain this distinction between embryonic and vegetative patterns of plant development. Mutant embryos are phenotypically abnormal, occasionally viviparous, and intolerant of desiccation. Mutant cotyledons produce trichomes characteristic of leaves, lack embryo-specific protein bodies, and exhibit a vascular pattern intermediate between that of leaves and cotyledons. These results suggest that lec cotyledons are partially transformed into leaves and that the wild-type gene (LEC) functions to activate a wide range of embryo-specific pathways in higher plants.
RESUMO
We have previously isolated and characterized over 90 recessive mutants of Arabidopsis thaliana defective in embryo development. These emb mutants have been shown to differ in lethal phase, extent of abnormal development, and response in culture. We demonstrate in this report the value and efficiency of mapping emb genes relative to visible and molecular markers. Sixteen genes essential for embryo development were mapped relative to visible markers by analyzing progeny of selfed F1 plants. Embryonic lethals are now the most common type of visible marker included on the linkage map of Arabidopsis. Backcrosses were used in several cases to orient genes relative to adjacent markers. Three genes were located to chromosome arms with telotrisomics by screening for a reduction in the percentage of aborted seeds produced by F1 plants. A restriction fragment length polymorphism (RFLP) mapping strategy that utilizes pooled EMB/EMB F2 plants was devised to increase the efficiency of mapping embryonic lethals relative to molecular markers. This strategy was tested by demonstrating that the bio1 locus of Arabidopsis is within 0.5 cM of an existing RFLP marker. Mapping embryonic lethals with both visible and molecular markers may therefore help to identify large numbers of genes with essential functions in Arabidopsis.
Assuntos
Plantas/genética , Southern Blotting , DNA/genética , Genes Letais , Ligação Genética , Mutação , Desenvolvimento Vegetal , Polimorfismo de Fragmento de RestriçãoRESUMO
Lethal mutants have been used in a variety of animal systems to study the genetic control of morphogenesis and differentiation. Abnormal development has been shown in some cases to be caused by defects in basic cellular processes. We describe in this report an embryo-lethal mutant of Arabidopsis thaliana that can be rescued by the addition of biotin to arrested embryos cultured in vitro and to mutant plants grown in soil. Mutant plants rescued in culture produced phenotypically normal seeds when supplemented with biotin but became chlorotic and failed to produce fertile flowers in the absence of biotin. Arrested embryos were also rescued by desthiobiotin, the immediate precursor of biotin in bacteria. Langridge proposed 30 years ago (1958, Aust. J. Biol. Sci. 11, 58-68) that the scarcity of plant auxotrophs might be caused by lethality prior to germination. The bio1 mutant of Arabidopsis described in this report clearly demonstrates that some auxotrophs in higher plants are eliminated through embryonic lethality. Further analysis of this mutant should provide valuable information on the nature of plant auxotrophs, the biosynthesis and utilization of biotin in plants, and the underlying causes of developmental arrest in lethal mutants of Arabidopsis.
Assuntos
Biotina/fisiologia , Genes Letais , Mutação , Plantas/genética , Biotina/análogos & derivados , Biotina/farmacologia , Técnicas de Cultura , Homozigoto , Desenvolvimento Vegetal , Plantas/embriologia , Sementes/efeitos dos fármacos , Sementes/fisiologiaRESUMO
Arrested embryos from lethal (emb) mutants of Arabidopsis thaliana were rescued on a nutrient medium designed to promote plant regeneration from immature wild-type cotyledons. The best response was observed with mutant embryos arrested at the heart to cotyledon stages of development. Embryos arrested at a globular stage produced callus but failed to turn green or form normal shoots in culture. Many of the mutant plants produced in culture were unusually pale with abnormal leaves, rosettes, and patterns of reproductive development. Other plants were phenotypically normal except for the presence of siliques containing 100% aborted seeds following self-pollination. These results demonstrate that genes with essential functions during plant embryo development differ in their pattern of expression at later stages of the life cycle. Most of the 15 genes examined in this study were essential for embryogenesis but were required again for subsequent stages of development. Only EMB24 appeared to be limited in function to embryo development. These differences in the response of mutant embryos in culture may facilitate the classification of embryonic lethals and the identification of genes with developmental rather than housekeeping functions.
RESUMO
Wild-type plants of Arabidopsis thaliana strain "Columbia" regenerated at a high frequency from immature cotyledons cultured on a shoot-inducing medium containing 1.0 mg/l 6-benzylaminopurine and 0.1 mg/l 1-naphthaleneacetic acid. Cotyledon segments expanded rapidly and produced numerous shoots after 2-3 weeks in culture. Regeneration occurred in the absence of the original shoot apex. Hypocotyl segments from immature embryos produced root hairs and callus in culture but only rarely developed shoots. Hygromycin, kanamycin and G-418 inhibited cotyledon expansion and shoot formation in culture. Vancomycin was much less toxic to cotyledon segments than either carbenicillin or cefotaxime. Immature cotyledons therefore yield numerous regenerated plants that may be useful in future transformation studies.
RESUMO
Analysis of the expression of genes encoding the beta-conglycinin seed storage proteins in soybean has been used to extend our understanding of developmental gene expression in plants. The alpha, alpha', and beta subunits of beta-conglycinin are encoded by a multigene family which is organ-specific in its expression. In this study we report the differentially programmed accumulation of the alpha, alpha', and beta subunits of beta-conglycinin. Multiple isomeric forms of each subunit are present in the dry seed, but the timing of their accumulation is unique for each subunit. The previously reported variation in amount of alpha' and alpha subunits in axis and cotyledons is also reflected in the amount of subunit specific mRNA which is present in each tissue. The beta subunit, previously undetected in soybean axes, is found to be synthesized but rapidly degraded. These differences in beta-conglycinin protein accumulation may be reflected by the morphological differences observed in protein bodies between these two tissues.
RESUMO
The major storage proteins isolated from wild-type seeds of Arabidopsis thaliana (L.) Heynh., strain "Columbia", were studied by sucrose gradient centrifugation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Both the hypocotyl and cotyledons of mature embryos contained abundant 12 S (cruciferin) and 2 S (arabin) proteins that appeared similar in size and subunit composition to the cruciferin (12 S) and napin (1.7 S) seed-storage proteins of Brassica napus. The 12 S protein from Arabidopsis was resolved by SDS-PAGE into two groups of subunits with approximate relative molecular weights of 22-23 kDa (kilodalton) and 30-34 kDa. These polypeptides accumulated late in embryo development, disappeared early in germination, and were not detected in other vegetative or reproductive tissues. Accumulation of the 12 S proteins in aborted seeds from nine embryo-lethal mutants with different patterns of abnormal development was studied to determine the extent of cellular differentiation in arrested embryos from each mutant line. Abundant 12 S proteins were found in arrested embryos from two mutants with late lethal phases, but not in seven other mutants with lethal phases ranging from the globular to the cotyledon stages of embryo development. These results indicate that the accumulation of seed-storage proteins in wild-type embryos of Arabidopsis is closely tied to morphogenetic changes that occur during embryo development. Embryo-lethal mutants may therefore be useful in future studies on the developmental regulation of storage-protein synthesis.