The Light Reflection Changes of Monolithic Zirconia and Lithium Disilicate after Using Two External Staining Kits following by Thermocycling.

BACKGROUND: The purpose of the study was to evaluate the changes of light reflection% on two materials (monolithic zirconia and lithium disilicate) after using two external staining kits following by thermocycling. METHODS: Specimens were sectioned from monolithic zirconia (n = 60) and lithium disilicate (n = 60) then divided into six groups (n = 20). Two different types of external staining kits were used and applied to the specimens. The light reflection% was measured before staining, after staining and after thermocycling using a spectrophotometer. RESULTS: The light reflection% of zirconia was significantly higher compared to lithium disilicate at the beginning of the study (p = 0.005), after staining with kit 1 (p = 0.005) and kit 2 (p = 0.005) and after thermocycling (p = 0.005). For both materials, the light reflection% was lower after staining with Kit 1 compared to kit 2 (p < 0.043). After thermocycling, the light reflection% of lithium disilicate increased (p = 0.027) and was unchanged with Zirconia (p = 0.527). CONCLUSIONS: There is a difference between the materials regarding light reflection% as the monolithic zirconia showed higher light reflection% comparing lithium disilicate throughout the entire experiment. For lithium disilicate, we recommend using kit 1 as we found that, after thermocycling, the light reflection% of kit 2 was increased.

Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro.

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.

The Effect of Aging on the Roughness and Bacterial Adhesion of Lithium Disilicate and Zirconia Ceramics.

PURPOSE: This in vitro study was designed to compare bacterial adhesion to zirconia versus lithium disilicate crowns after artificial aging. MATERIALS AND METHODS: Seventy-five discs were tested in this study: 25 Cr-Co base metal discs (control), 25 zirconia discs, and 25 lithium disilicate discs. Each sample was polished and glazed appropriately. The discs were subjected to 1000 thermal cycles between 5 and 55°C, whereby samples stayed in the bath for 20 seconds, at each temperature. Then, 10 µL of Streptococcus sanguinis suspension was placed on the discs. The samples were scanned for surface roughness before and after aging, and after incubation. Sp and Sa were measured with a confocal, visible light disk-scanning system (Sa expresses the difference in height of each point compared to the arithmetical mean of the surface. Sp is the height of the highest peak within the defined area). Optical density of the discs was evaluated by a spectrophotometer. One-way ANOVA was performed to assess differences after aging and incubation. RESULTS: Statistical analysis showed significant differences (p = 0.02) in surface roughness between the Cr-Co base metal, zirconia and lithium disilicate before and after aging. The mean Sa was 0.36 ± 0.12 µm, 0.638 ± 0.24 µm, and 1.23 ± 0.42 µm, respectively. Lithium disilicate had the highest surface roughness values. There was a significant difference (p = 0.001) after incubation. The mean and standard deviation surface roughness of Cr-Co base metal, zirconia and lithium disilicate were 0.99 ± 0.49 µm, 1.40 ± 0.46 µm, and 2.44 ± 1.21 µm, respectively. While no significant differences were found in the accumulation of S. sanguinis between zirconia and lithium disilicate, there was a significant difference (p = 0.02) in the optical density between these 2 test groups and the control group (metal). The optical density of metal (0.94 ± 0.15) was higher than for lithium disilicate (0.74 ± 0.10) and zirconia (0.75 ± 0.08). There was no statistical difference in bacterial adherence between lithium disilicate and zirconia. CONCLUSION: Dental crown materials differed significantly in terms of surface roughness and bacterial adhesion between Cr-Co base metal and zirconia and lithium disilicate. After aging and bacterial adherence, the zirconia discs had the smoothest surface, with similar bacterial accumulation as lithium disilicate; suggesting that lithium disilicate may be less sensitive to bacterial adhesion than zirconia.

An in vitro evaluation of marginal fit zirconia crowns fabricated by a CAD-CAM dental laboratory and a milling center.

BACKGROUND: Marginal fit is critical for the success and longevity of a dental restoration. Zirconia crowns can be fabricated either chair-side, in a dental laboratory or in a milling center; each can give different marginal fits results. However, discussion of the marginal fit of zirconia crowns when different fabrication methods are compared is lacking in the literature. PURPOSE: To compare the marginal discrepancy (MD) and absolute marginal discrepancy (AMD) of computer-aided design, and computer-aided manufacturing (CAD-CAM) used in a dental laboratory and a milling center for producing monolithic zirconia crowns. METHODS: The marginal fit of 30 zirconia crowns cemented to typodont teeth was evaluated by means of a sectioning technique. Fifteen crowns were fabricated with a CEREC inLAB MC X5 from IPS e.max ZirCAD blocks. Fifteen crowns were fabricated using a LAVA milling center from LAVA Plus Zirconia Blocks. The 30 crowns were sectioned with a precision saw, and MD and AMD were subsequently measured using a light microscope. Data were analyzed using the one-way ANOVA technique to investigate significant differences in the marginal fit between the two fabrication systems (α = .05). RESULTS: The AMD dimension of the CEREC inLAB system was significantly smaller (P < .05). Mean AMD values for zirconia crowns fabricated by the CEREC inLAB were 85 µm, and for the LAVA milling center 133 µm. There was no significant difference between the two systems regarding the MD dimensions. The MD values for zirconia crowns fabricated by the CEREC inLAB were 53 µm and for the LAVA milling center 61 µm. CONCLUSIONS: The CEREC inLAB system demonstrated significantly better marginal fit in relation to the AMD. However, no difference between the systems was found in the MD. Monolithic zirconia crowns fabricated by the CAD-CAM CEREC inLAB system and the LAVA system milling center showed MD values of less than 120 µm, which is within the clinically acceptable range.

Comparison of marginal fit between CAD-CAM and hot-press lithium disilicate crowns.

STATEMENT OF PROBLEM: Hot-pressing and computer-aided design and computer-aided manufacturing (CAD-CAM) are major techniques for the fabrication of lithium disilicate crowns. They exhibit different accuracies regarding marginal fit, an important factor in restoration survival. However, studies comparing the marginal fit of different fabrication methods are lacking. PURPOSE: The purpose of this in vitro study was to compare the marginal discrepancy (MD) and absolute marginal discrepancy (AMD) of lithium disilicate crowns produced by the hot-press and CAD-CAM techniques. MATERIAL AND METHODS: Thirty typodont teeth were divided into 2 groups. Fifteen teeth were scanned with the CEREC Omnicam intraoral scanner, and crowns were fabricated with the CEREC MC XL chairside CAD-CAM milling unit from IPS e.max CAD blocks. Fifteen typodont teeth were sent to a dental laboratory, and lithium disilicate crowns were fabricated from IPS e.max press ingots using the hot-press technique. The 30 crowns were cemented and then sectioned with a precision saw. The MD and AMD were measured for each crown with a light microscope. One-way ANOVA was conducted to analyze significant differences in crown marginal fit between the fabrication systems (α=.05). RESULTS: For the CAD-CAM technique, the mean values of the AMD measurements were 115 µm, and for the hot-press technique, 130 µm. The MD measurements were 87 µm for the CAD-CAM technique and 90 µm for the hot-press technique. One-way ANOVA revealed no significant differences between the fabrication methods regarding marginal fit (P>.05). CONCLUSIONS: No significant differences were found between the fabrication methods tested. Both the CAD-CAM and hot-press techniques for producing monolithic lithium disilicate crowns produced MD values of less than 120 µm, within the clinically acceptable range.

Predicting the clinical performance of dental students with a manual dexterity test.

Dentists must be skilled when using dental mirrors. Working with mirrors requires spatial perception, bimanual coordination, perceptual learning and fine motor skills. Many studies have attempted to determine the predictors of manual skills among pre-clinical students, but consensus has yet to be reached. We hypothesized that valid and reliable occupational therapy test performance regarding indirect vision would differ between dental students and junior dentists and would explain the variance in manual skill performance in pre-clinical courses. To test this hypothesis, we applied the Purdue Pegboard test and O'Connor Tweezer Dexterity test under different conditions of direct and indirect vision. We administered these tests to students in phantom-head academic courses in 2015 and 2016 and to junior dentists. Students performed the tests at three time points: before phantom training (T0), at the end of the training (T1) and in the middle of the following year of study (T2). Dentists performed the same tests twice at 1st and 2nd trials one week apart. The results showed that indirect tasks were significantly more difficult to perform for both groups. These dexterity tests were sensitive enough to detect students' improvement after phantom training. The dentists' performances were significantly better than those of students at T0, specifically with regard to the use of tweezers under direct and indirect vision (the O'Connor test). A regression analysis showed that students' manual grades obtained at the beginning of the phantom course, their performance on the Purdue test using both hands, and their performance on the O'Connor test under indirect vision predicted phantom course success in 80% of cases. The O'Connor test under indirect vision is the most informative means of monitoring and predicting the manual skills required in the pre-clinical year of dentistry studies.

Direct Contact Test for Evaluating Bacterial Growth on Machined and Rough Surface Implants: An In Vitro Study.

OBJECTIVE: To use a modified direct contact test (DCT) to evaluate the bacterial growth on 3 commercially available implants. MATERIALS AND METHODS: Streptococcus salivarius growth was tested on 1 machined surface and 2 commercial rough surface implants. Seven implants from each group were fixed to the cover of a 96-well microtiter plate. Bacterial suspension was placed on each implant, and the plate was incubated at 37°C for 1 hour to allow the suspension fluid to evaporate. Fresh medium was added to 7 corresponding wells in the microtiter plate, and the cover holding the implants was placed on the plate to enable immersion of the implant. Noncontaminated implant samples served as control. Bacterial growth was monitored spectrophotometrically at 650 nm for 24 hours. RESULTS: There was a significant S. salivarius growth in all groups of test implants compared with the control. There was no significant difference between bacterial growth kinetics on treated and nontreated implant surfaces (P = 0.241). CONCLUSION: Using the DCT, we found no difference in bacterial growth between machined and rough surface implants.

Collaboration Patterns and Processes Between Dentists and Dental Laboratories When Planning and Fabricating Implant-Supported Restorations.

INTRODUCTION: The success of implant-supported restorations is dependent on proper treatment planning, effective communication within the clinical team, and the use of appropriate methods and materials in the dental laboratory. The objective of this study was to determine collaboration trends between dentists and laboratories and to assess the common methods and materials involved in fabricating implant-supported restorations. METHODS: Questionnaires were distributed to dental laboratories and technicians. Seventy questionnaires were answered and were included in the data analyses. RESULTS: Most of the impressions (87%) were taken using an individual custom-made open tray. In 83% of impressions, screw-retained transfer units were used, and in 61% of cases, the units were splinted. Bite registration was recorded in 91% of the cases. In 80% of cases, articulator setup was done. When matching the shade of a restoration in the anterior, 57% of the technicians do so in collaboration with the dentist, and 39% match the shade independently. Type of restoration and abutment selection were done mainly by the technicians. Abutment selection was reported to be carried out by 72% of the technicians. CONCLUSIONS: Generally, dentists and technicians follow the standards recommended in the contemporary literature, especially, in major procedures such as impression taking, bite registration, and articulator setup. However, principal decisions, such as abutment and color shade are done mainly by technicians.

Consequences of experience and specialist training on the fabrication of implant-supported prostheses: A survey.

STATEMENT OF PROBLEM: Fabricating fixed restorations on implants requires that dentists stay up-to-date with the scientific publications. MATERIAL AND METHODS: A questionnaire containing 29 questions was sent to dentists, members of the Israeli Society of Prosthodontics, and university faculty members. Differences were evaluated using the chi-square and Fisher exact tests and Mann-Whitney U and Wilcoxon tests for questions enquiring into the frequency of use of certain methods (α=.05). RESULTS: A total of 155 questionnaires were completed by 84 general dental practitioners and 71 specialists and residents. Statistical differences (P<.05) were found in the course of the fabrication of the implant-supported prostheses in the diagnostic waxing, trial restoration, and impression-making processes. Trial restorations were often used by residents and specialists before starting an esthetic restoration. Diagnostic waxing was used when treating patients with complex esthetic needs, mainly by prosthodontists (P=.019). A custom tray for impression making was used more by residents and specialists than by general dental practitioners (P<.001). The open tray technique for impression making of single or up to 3 implants was performed mainly by residents, specialists, and general dental practitioners with professional experience of fewer than 15 years. General dental practitioners used primarily plastic or metal trays for impression making. CONCLUSIONS: Significantly, impression techniques and the use of custom open trays, diagnostic waxing, and trial restorations were performed by residents, specialists, and dentists with fewer than 15 years of experience.

A Comparison of Cone-beam Computed Tomography with Periapical Radiography in the Detection of Separated Instruments Retained in the Apical Third of Root Canal-filled Teeth.

INTRODUCTION: This study compared the diagnostic efficacies of cone-beam computed tomographic (CBCT) imaging and periapical radiography (PR) in the detection of retained separated instruments located at the apical third of filled root canals. METHODS: Sixty single-rooted extracted human teeth were instrumented to size #25 and were randomly divided to a simulated 2-mm #30 K-file (stainless steel or nickel-titanium) segment separation at the apical third of the canal (n = 40) or a control group without a separated instrument (n = 20). The canals were obturated to the separated instrument or the working length for the teeth without an instrument using gutta-percha with AH26 (Dentsply DeTrey GmbH, Konstanz, Germany) or Roth sealer (Roth International Ltd, Chicago, IL). The teeth were invested in a mandible model simulating the bone density and imaged using CBCT imaging and PR. The images were evaluated separately by 2 calibrated observers twice with an interval of 4 weeks. Cohen kappa was used to evaluate the observer agreement. Receiver operating characteristic curve analysis was used to evaluate the discrimination ability. RESULTS: The intraobserver kappa was 0.744 and 0.627, and between the observers, it was 0.593 and 0.275 for PR and CBCT imaging, respectively. Using PR, the mean sensitivity was 71.25%, and the specificity was 93.75%. Using CBCT imaging, the sensitivity and specificity were 41.25% and 71.25%, respectively. Although for PR the area under the curve values ranged between 0.75 and 0.91 (P < .05), for CBCT they ranged between 0.48 and 0.60 (P > .05), regardless of the instrument or the sealer type. CONCLUSIONS: PR performed better than CBCT imaging for the detection of retained separated instruments located at the apical third of extracted human root canal-filled teeth.