Green synthesis of zinc oxide nanoparticles using Brassica oleracea var. botrytis leaf extract: Photocatalytic, antimicrobial and larvicidal activity.

Green synthesis of nanomaterials has emerged as an ecofriendly sustainable technology for the removal of dyes in the last few decades. Especially, plant leaf extracts have been considered as inexpensive and effective materials for the synthesis of nanoparticles. In this study, zinc oxide nanoparticles (ZnO NPs) were prepared using leaves extract of Brassica oleracea var. botrytis (BO) by co-precipitation and applied for photocatalytic/antibacterial activity. The synthesized BO-ZnO NPs was characterized by different instrumental techniques. The UV-vis Spectrum of the synthesized material showed maximum absorbance at a wavelength of 311 nm, which confirmed the formation of BO-ZnO NPs. The XRD pattern of BO-ZnO NPs represents a hexagonal wurtzite structure and the average size of particles was about 52 nm. FT-IR spectrum analysis confirms the presence of hydroxyl, carbonyl, carboxylic, and phenol groups. SEM images exhibited a flower like morphology and EDX spectrum confirming the presence of the elements Zn and O. Photo-catalytic activity of BO-ZnO NPs was tested against thiazine dye (methylene blue-MB) degradation under direct sunlight irradiation. Around 80% of the MB dye got degraded at pH 8 under 75 min of sunlight irradiation. Further, the study examined that the antimicrobial and larvicidal activity of BO-ZnO NPs obtained through green synthesis. The antimicrobial study results showed that the BO-ZnO NPs formed zones against bacterial pathogens. The results showed the formation of an inhibition zone against B. subtills (16 mm), S.aureus (13 mm), K. pneumonia (13 mm), and E. coli (9 mm) respectively at a concentration of 100 µg/mL of BO-ZnO NPs. The larvicidal activity of the BO-ZnO NPs was tested against the fourth instar of Culex quinquefasciatus mosquito larvae The LC50 and LC90 values estimated through the larvicidal activity of BO-ZnO NPs were 76.03, 190.03 ppm respectively. Hence the above findings propose the synthesized BO-ZnO NPs by the ecofriendly method can be used for various environmental and antipathogenic applications.

Using fish scales as a new biosorbent for adsorption of nickel and copper ions from wastewater and investigating the effects of electric and magnetic fields on the adsorption process.

In this study, fish scales (Pomadasys kaakan's scales) were used as new biosorbent for removing Ni2+ and Cu2+ ions from wastewater. The effects of electric and magnetic fields on the absorption efficiency were also investigated. The effects of sorbent content, ion concentration, contact time, pH, electric field (EF), and magnetic field (MF) on absorption efficiency were assertained. In addition, the isotherm of absorption was studied in this work. This study revealed that electric field and magnetic field have significant effects on the absorption efficiency of ions from wastewater. An increase in the electric field enhanced the removal percentage of the ions and accelerated the absorption process by up to 40% in comparison with the same condition without an electric field or a magnetic field. By increasing contact time from 10 to 120 min, the removal of Ni2+ ions was increased from 1% to 40% and for Cu2+ ions, the removal increased from 20% to almost 95%, respectively. In addition, increasing pH, ion concentration and scales dose increased removal percentage effectively. The results indicated that using fish scales for Cu2+ ions absorption is ideal due to the very high removal percentage (approximately 95%) without using either an electric or magnetic field.

A review on carbon-based molecularly-imprinted polymers (CBMIP) for detection of hazardous pollutants in aqueous solutions.

This article discusses the unique properties and performance of carbon-based molecularly-imprinted polymers (MIPs) for detecting hazardous pollutants in aqueous solutions. Although MIPs have several advantages such as specific recognition sites, selectivity, and stability, they suffer from a series of drawbacks, including loss of conductivity, electrocatalytic activity, and cost, which limit their use in various fields. Carbon-based MIPs, which utilize carbon electrodes, carbon nanoparticles, carbon dots, carbon nanotubes, and graphene substrates, have been the focus of research in recent years to enhance their properties and remove their weaknesses as much as possible. These carbon-based nanomaterials have excellent sensitivity and specificity for molecular identification. As a result, they have been widely used in various applications, such as assessing the environmental, biological, and food samples. This article examines the growth of carbon-based MIPs and their environmental applications.

Supplementation of Estradiol Into Semen Extender Improved Goat Sperm Cryopreservation.

Estradiol is a steroid hormone excreted from the female gonads, mainly during the pre-estrus. However, the potential effects of estradiol are yet to be explored on sperm parameters through cryopreservation. In this study, we supplemented estradiol, 3 and 5 µM, in the goat semen extender and assessed the sperm parameters after a freeze-thawing process. Sperm motility was assessed using the computer-assisted sperm analysis system. Sperm viability and membrane integrity improved using both 3 and 5 µM concentrations of estradiol. The highest rate of progressive motility was observed in the 3 µM estradiol group. However, a higher concentration of estradiol (5 µM) reduced the progressive motility. Then, we were interested to see if the supportive effect of estradiol on sperm motility is mediated through the intracellular concentration of calcium ionophore. We supplemented the semen extender with 1 and 10 mM ethylenediaminetetraacetic acid (EDTA) and showed that 1 mM has no adverse effect on progressive sperm motility. Then, estradiol (3 µM) was supplemented with or without EDTA (1 mM) into the semen extender. Individual EDTA treatment improved the progressive sperm motility compared to the control group. However, in the presence of estradiol, EDTA treatment reduced the progressive motility compared to the individual estradiol group. This indicated a considerable interaction between estradiol and EDTA for progressive sperm motility. Indeed, EDTA reduced the supportive effects of estradiol on sperm cryopreservation parameters. These results indicated that induction of higher progressive sperm motility in response to estradiol is a calcium-dependent process, as the EDTA did completely abrogate the estradiol-mediated effect.