Exploring alternatives for securing anatomical structures in capturing digital images: A comparative analysis.

Teaching veterinary anatomy using digital platforms requires improved image quality, which may influence the fixation process. This study aimed to compare four embalming solutions for high-colour-quality images of different tissues compared to the original image. Four equine left pelvic limbs were cut into metameres and divided equally for application of 10% formaldehyde, 96% glycerine, 33% hypersaturated NaCl solution and modified Larssen solution, respectively, which was maintained for 3 days. After drying for 3 days at room temperature, photographs were obtained at time 0 (T0), without any fixation process (original colour); time 1 (T1), immediately after removal from the solutions; and every 24 h for 3 days (T2-T4). The image colour quality was investigated by digitally evaluating the cortical bone, tendon and bone marrow using histograms and CIEDE2000 as well as by 10 specialists in an online survey. CIEDE2000 and histograms revealed that all fixation solutions changed the original tissue colour at all the time points (p < 0.0001). According to the specialists, the 33% saline solution produced the best results compared to the original one. The modified Larssen solution demonstrated better results for the tendon, marrow and cortical bone at T3 (p = 0.0015). Considering the colour of digital images, the modified Larssen solution provided the best results; however, the visual evaluation by the specialists revealed the 33% saline solution as the best.

Horse behavior and facial movements in relation to food rewards.

Food rewards are believed to have a positive valence in horses. The aim of this study was to assess the effect of food rewards on horse behavior before entering a horse chute, and behavior and facial movements while restrained in it. Thirteen female adult horses were brought once daily to an animal handling facility for three weeks. In week 1, baseline period, no reinforcement was applied. In weeks 2 and 3, experimental phase, half of the horses received positive reinforcement treatment after entering and remaining in the chute; the remaining horses were considered as controls (no positive reinforcement applied). There was a cross-over between the groups during the experimental phase. Horses were individually brought to the restraining chute and videos recorded during 60-sec. The duration and number of entries into the area close to the gate leading to the chute were measured before restraining and body posture, neck position, and tail swinging were recorded in the chute. Facial movements were also recorded and scored using EquiFACS methodology. Multilevel linear and logistic models were built to assess behavioral changes from baseline to the treatment phase and between phases (control and positively reinforced). Horses did not change their body posture or tail swings across the different phases (P > 0.1) and were less likely to show lowered neck during the positively reinforced phase (OR: 0.05; CI95%: 0.00-0.56; P = 0.05) compared to baseline. The likelihood of a lowered neck did not differ between the positive reinforcement and control phases (P = 0.11). In the positively reinforced phase, horses seemed to be more attentive (ears forward) and active (less eye closures, more nose movements) than in the control phase. A three-day positive reinforcement phase did not elicit major changes in body behavior in the chute but affected the facial movements of group-housed mares.

Prevalence of Juvenile Osteochondral Conditions in Yearling Thoroughbred Racehorses in Southern Brazil.

This study aimed to investigate the prevalence of juvenile osteochondral conditions (JOCCs) in yearling Thoroughbred (TB) racehorses in Southern Brazil, as well as to examine the same animals studied as weanlings by Bastos et al. (2017) and compare the radiographic findings for the two ages. Radiographs of 76 male and female TB yearlings from Paraná State, Southern Brazil, were investigated. The proximal interphalangeal, metacarpophalangeal/metatarsophalangeal (MC/MT), tarsal, and femorotibial (FT) joints were evaluated using 24 radiographic projections. The evaluation consisted of a severity index (rated as 0, 1, 2, 4, or 8), the sum of which resulted in an osteoarticular status (OAS) of good, intermediate, or poor for each animal. Radiographs of 92% of the investigated animals presented at least one finding consistent with JOCCs. The most affected region was the tarsal joint (72.9%), followed by the FT (50%), MT (25%), and MC (23%) joints. Thirty-three (43.4%) horses presented a decline in their OAS, whereas nine (11.8%) presented an improvement from the weanling to yearling age. The radiographic findings suggest that JOCCs are frequent in TB yearlings, but the meaning and relevance of these radiographic findings require further study.

Infrared Thermography Applied to Monitoring Musculoskeletal Adaptation to Training in Thoroughbred Race Horses.

Infrared thermography has been used to help in diagnosing lameness. It is hypothesized that, if used in a routine basis, it could help in understanding musculoskeletal modifications during race training. This study aimed to evaluate thermal variation in the musculoskeletal regions of young Thoroughbred (TB) horses during their initial months of race training. Thermographic examinations were performed once every 2 weeks on 16 (10 male, 6 female) two-year-old TB racehorses, from arrival to the racetrack in June 2016, until January 2017, for a total of 16 evaluations. Thermographic imaging was performed using the appropriate protocol. Temperature (°C) was measured at the dorsal and palmar/plantar aspects of specific regions of interest (fetlock, metacarpal, metatarsal, carpal, tarsal, thoracolumbar, sacroiliac spine, and both hips). Initially, we found a thermal balance and all regions demonstrated a positive correlation with one another. However, a significant difference was noted between the left and right sides as training progressed. Four horses were withdrawn from the study after 50% of evaluations because of metacarpal conditions associated with training. Thermographic examination revealed changes before the clinical manifestation of these conditions. In conclusion, this study demonstrated that infrared thermography is an image technique that can facilitate understanding of musculoskeletal system modifications to race training and should be further investigated as a predictive tool to anticipate the occurrence of lesions.

Animal-Assisted Activity for Children with Autism Spectrum Disorder: Parents' and Therapists' Perception.

Autism spectrum disorder (ASD), characterized by communication and social interaction difficulties, might be positively influenced by animal-assisted activity (AAA). The aim of this study was to investigate the parents' and therapists' perception to behavior in children with ASD after they underwent AAA. The study was performed in a specialized therapeutic center in Curitiba, South of Brazil. A total of 15 children (14 boys and 1 girl), aged 5.6 ± 1.6 years, diagnosed with ASD, underwent at least 10 weekly 30-min AAA sessions. Three trained dogs were used in total, and there were two dogs in each session. Children were free to interact with dogs and among themselves, in groups of 11.2 ± 2.2 children per session, in the presence of therapists, the researcher, and a veterinarian observing the dogs and ensuring safety. The parents and therapists completed two questionnaires on their perception of the children's behavior before and after AAA. A total of 18 visits were necessary to complete the 10 sessions for each of the 15 children. According to the therapists' perception, AAA increased positive gestures and facial expression in children (p = 0.010) and improved peer interaction. Even though there was a 50% reduction in the parents' feedback at the end of the study, according to their perception there was a reduction in self-aggression and repetitive stereotyped movements, as well as an improvement in speech communication and creativity. This study demonstrated the positive effects of the AAA on the social behavior of children with ASD.

Pressuring of Acupoints as a Complement to the Diagnosis of Stifle Diseases in Horses.

Diseases of the stifle joint remain a challenge for veterinarians. The objective of this study was to achieve a valuable acupuncture suggestive diagnosis to be considered for stifle joint diseases in horses. Thirty-nine nonlame horses involved in different activities were assessed. Acupuncture was independently performed by two evaluators. Reactions of the animal when pressurizing the point suggestive of stifle disease (PSSD), Bladder-20 and/or Bladder-21, were considered as the inclusion criteria for inclusion in the stifle group (SG, n = 31), and the animals with no reactions were assigned to the control group (n = 8). Radiographic and ultrasonographic examinations were performed and evaluated by two independent professionals blinded to the group allocation. Thermographic examination of the PSSD and stifles was also performed, after acclimatization. The ultrasound scores and radiographic findings were higher in the SG than in the control group. Thermography evidenced increased temperature in the PSSD and stifles in the SG. The minimum acupuncture diagnostic criteria for stifle joint disease had a sensitivity of 87.5% and a specificity of 57.0%, and the addition of the acupoints Gallbladder-dorsal tuber coxae, Gallbladder-27, and Spleen-13 to the minimum diagnostic criteria improved sensitivity and specificity. In conclusion, assessing the reaction at the demonstrated acupoints can facilitate a diagnosis of a potential stifle lesion.

Collection, processing and freezing of equine bone marrow cells.

There is no consensus on aspects of equine bone marrow collection and processing. The study aimed to describe the collection of large volumes of bone marrow from horses of advanced age, with emphasis on bone marrow mononuclear cells (BMMCs) recovery and viability after cryopreservation. Fourteen horses, aged 3-24 years, were divided into three experiments. E1 studied the feasibility of collecting 200 mL from the sternums of horses of advanced age; E2 examined the number of cells obtained from the first and last syringe of each puncture; and E3 investigated the influence of heparin concentration on the prevention of cell aggregation, and cell viability after freezing in liquid nitrogen. Bone marrow aspirations were done with syringes pre-filled with Iscove's modified Dulbecco's medium and different concentrations of sodium heparin. BMMCs were counted, cell viability was determined, and samples were frozen. Bone marrow collection from the sternum is safe, even at large volumes and from horses of advanced age, and the number of cells recovered decreases with successive aspirations (p < 0.0001). Heparin concentration influenced cell aggregation, and recovered cells continued to be commercially viable after 150 days in frozen storage.

Intratracheal therapy with autologous bone marrow-derived mononuclear cells reduces airway inflammation in horses with recurrent airway obstruction.

This research evaluated the effects of bone marrow-derived mononuclear cells (BMMCs) on the inflammatory process in the equine recurrent airway obstruction (RAO). Eight horses in RAO clinical score were divided into cell therapy group (Gcel) treated with a single intratracheal dose of BMMCs, and dexamethasone group (Gdex) treated with 21days of oral dexamethasone. The horses were clinically revaluated on days 7 and 21, together with cytological evaluation of the BALF, and detection of inflammatory markers (interleukins [IL]-10, -4, and -17, and interferon γ and α). There were decreases in respiratory effort and clinical score on days 7 and 21(p<0.05) for both groups. The percentage of neutrophils decreased and macrophages increased on days 7 and 21 (p<0.005) in both groups. IL-10 levels increased in the Gcel group on day 21 compared to days 0 and 7 (p<0.05), but this was not observed in the Gdex group. The quantification of IL-4, IL-17, IFN-γ, and IFN-α did not change between evaluations in both groups. These preliminary results suggest that BMMCs may ameliorate the inflammatory response of RAO.

Pulmonary inflammation due to exercise-induced pulmonary haemorrhage in Thoroughbred colts during race training.

This study investigated the putative roles of inflammation and platelet-activating factor (PAF) in exercise-induced pulmonary haemorrhage (EIPH). Two-year-old Thoroughbred colts (n=37) were exercised on a racetrack for 5months before commencement of the study. Each colt was then exercised at 15-16m/s over 800-1000m and broncho-alveolar lavage fluid (BALF) was collected 24h later. The colts were subsequently divided into two groups on the basis of BALF analysis; an EIPH-positive group (presence of haemosiderophages, n=23) and an EIPH-negative group (absence of haemosiderophages, n=14). BALF from the EIPH-positive group had a significantly higher protein concentration (0.39±0.28 vs. 0.19±0.12mg/mL, P=0.031), higher PAF bioactivity (0.18±0.12 vs. 0.043±0.05 340:380nm ratio, P=0.042) and a higher lipid hydroperoxide concentration compared to the EIPH-negative group. There was also a lower nitrite concentration and reduced production of superoxide anion and hydrogen peroxide by alveolar macrophages in the EIPH-positive group. There was evidence of pulmonary inflammation and a decreased innate immune response of alveolar macrophages in EIPH-positive colts compared with the EIPH-negative group.