Indications of a ferromagnetic quantum critical point in [Formula: see text].

We investigate the previously observed superconductivity in ferromagnetic SmN in the context of the breakdown of order between two magnetic phases. Nitrogen vacancy doped SmN[Formula: see text] is a semiconductor which lies in the intermediary between ferromagnetic SmN and anti-ferromagnetic Sm. Optical data reported here corroborate the prediction that electrical transport is mediated by Sm 4f defect states, and electrical transport measurements characterise the metal-insulator transition over the doping range. Our measurements show that the superconducting state in nitrogen vacancy doped [Formula: see text] is the most robust near the breakdown of magnetic order, and indicate the location of a quantum critical point. Furthermore we provide additional evidence that the superconducting state is formed from majority spin electrons and thus of unconventional S = 1 type.

Extreme conditions reduce hatching success of green turtles (Chelonia mydas L.) at Karan Island, the major nesting site in the Arabian Gulf.

Survival in the early life stages is a major factor determining the growth and stability of wildlife populations. For sea turtles, nest location must provide favorable conditions to support embryonic development. Hatching success and incubation environment of green turtle eggs were examined in July 2019 at Karan Island, a major nesting site for the species in the Arabian Gulf. Mean hatching success averaged at 38.8 % (range = 2.5-75.0 %, n = 14). Eggs that suffered early embryonic death (EED) and late embryonic death (LED) represented 19.8 % (range: 3.3-64.2 %) and 41.4 % (range: 4.8-92.6 %) of the clutch on average, respectively. Nest sand was either coarse (0.5-1 mm: mean 44.8 %, range = 30.4-56.9 % by dry weight, n = 14) or medium (0.25-0.5 mm: mean 33.6 %, range = 12.0-45.5 % by dry weight, n = 14). Mean sand moisture (4.0 %, range = 3.2-4.9 %, n = 14) was at the lower margin for successful development. Hatching success was significantly higher in clutches with sand salinity <1500 EC.uS/cm (n = 5) than those above 2500 EC.uS/cm (n = 5). Mean clutch temperatures at 1200 h increased by an average of 5.4 °C during the 50-d post-oviposition from 31.2 °C to 36.6 °C. Embryos experienced lethally high temperatures in addition to impacts of other environmental factors (salinity, moisture, sand grain size), which was related to reduced hatching success. Conservation initiatives must consider the synergistic influence of the above parameters in formulating strategies to improve the overall resilience of the green turtle population in the Arabian Gulf to anthropogenic and climate change-related stressors.

Effect of ultrasound on bubble-particle interaction in quartz-amine flotation system.

In this study, the effect of ultrasound (US) on the quartz-amine flotation system was investigated in detail by considering various surface chemistry techniques. The effect of ultrasound on particle size, shape factor, and surface roughness were characterized by using Brunauer-Emmett-Teller (BET) surface area measurements and scanning electron microscopy (SEM) analyses. The contact angle and bubble-particle attachment time, as well as adsorption density measurements was carried out to evaluate the effect of ultrasound on quartz surface wetting ability. In addition, atomic force microscopy (AFM) analyses were conducted, and finally micro-flotation studies were performed. As a result, it was found that the micro-flotation recovery at 2 × 10-5 M dodecyl amine hydrochloride (DAH) concentration increased from 45.45% to 63.64% with 30 W ultrasonic application at conditioning step. However, the micro-flotation recovery decreased to 37.50% when the ultrasonic power increased to 150 W. The results showed some effect of ultrasound on particle size, particle shape, and surface roughness in some extent. The increase in the contact angle and the decrease in the bubble-particle attachment time were observed. A slightly high adsorption density was measured. All these show a positive effect of ultrasound on quartz flotation with amine as a collector.

Sex-related differences in muscle size explained by amplitudes of higher-threshold motor unit action potentials and muscle fibre typing.

AIM: To investigate the relationships between motor unit action potential amplitudes (MUAPAMP ), muscle cross-sectional area (mCSA) and composition (mEI), per cent myosin heavy chain (%MHC) areas and sex in the vastus lateralis (VL). METHODS: Ten males and 10 females performed a submaximal isometric trapezoid muscle action that included a linearly increasing, steady torque at 40% maximal voluntary contraction, and linearly decreasing segments. Surface electromyographic decomposition techniques were utilized to determine MUAPAMPS in relation to recruitment thresholds (RT). Ultrasound images were taken to quantify muscle mCSA and mEI. Muscle biopsies were collected to calculate %MHC areas. Y-intercepts and slopes were calculated for the MUAPAMP vs RT relationships for each subject. Independent-samples t tests and ANOVA models examined sex-related differences in mCSA, mEI, slopes and y-intercepts for the MUAPAMP vs RT relationships and %MHC areas. Correlations were performed among type IIA and total type II %MHC area, mCSA and the slopes and y-intercepts for the MUAPAMP vs RT relationships. RESULTS: Males exhibited greater slopes for the MUAPAMP vs RT relationships (P = .003), mCSA (P < .001) and type IIA %MHC (P = .011), whereas females had greater type I %MHC area (P = .010) and mEI (P = .024). The mCSA, type IIA and total II %MHC area variables were correlated (P < .001-.015, r = .596-.836) with the slopes from the MUAPAMP vs RT relationships. CONCLUSION: Sex-related differences in mCSA and MUAPAMPS of the higher-threshold MUs were likely the result of larger muscle fibres expressing type II characteristics for males.

Compartment Syndrome of the Flexor Compartment of the Arm Secondary to Pectoralis Major Tendon Rupture.

Case. Compartment syndrome following muscle rupture is a rare entity with few mentions in the literature. We present a case of pectoralis major rupture in a 38-year-old male that evolved into compartment syndrome of the anterior compartment of the arm. Rupture of the pectoralis is uncommon and most often occurs during weight lifting. Compartment syndrome secondary to this injury is extremely uncommon, with only one reported case in the pectoralis major itself and several cases of biceps compartment syndrome. Due to the potentially devastating consequences of a missed compartment syndrome, it is imperative that physicians maintain a high level of suspicion in patients with these unusual injuries presenting with severe swelling and pain.

Lauryl phosphate adsorption in the flotation of Bastnaesite, (Ce,La)FCO3.

Wetting characteristics and micro-flotation responses of bastnaesite were examined as a function of pH and at different levels of lauryl phosphate adsorption. Theoretical computations for the bastnaesite-lauryl phosphate system were calculated using the universal force field (UFF) and semiempirical quantum chemical methods. The interaction energy and frontier orbital results correlate remarkably well with the experimental contact angle and micro-flotation test results. The wetting characteristics of bastnaesite with adsorbed collector were examined using both contact angle measurements and molecular dynamics simulations (MDS). The adsorption isotherm at low levels of lauryl phosphate adsorption was established. Finally, the relationship between hydrophobicity and adsorption density was examined by MDS, and compared to the results with octyl hydroxamate at low collector concentrations.

The change in motor unit firing rates at de-recruitment relative to recruitment is correlated with type I myosin heavy chain isoform content of the vastus lateralis in vivo.

AIM: To investigate the change in motor unit (MU) firing rates (FR) at de-recruitment relative to recruitment and the relation to % type I myosin heavy chain isoform content (type I %MHC) of the vastus lateralis (VL) in vivo. METHODS: Ten subjects performed a 22-s submaximal isometric trapezoid muscle action that included a linearly increasing, steady force at 50% maximal voluntary contraction, and linearly decreasing segments. Surface electromyographic signals were collected from the VL and were decomposed into constituent MU action potentials trains. A tissue sample from the VL was taken to calculate type I %MHC. The y-intercepts and slopes were calculated for the changes (Δ) in FR at de-recruitment (FRDEREC ) relative to FR at recruitment (FRREC ) vs. FRREC relationship for each subject. Correlations were performed between the y-intercepts and slopes with type I %MHC. RESULTS: The majority of MUs had greater FRDEREC than FRREC . The y-intercepts (r = -0.600, P = 0.067) were not significantly correlated, but the slopes (r = -0.793, P = 0.006) were significantly correlated with type I %MHC. CONCLUSION: The majority of the motoneuron pool had greater FRDEREC than FRREC , however, individuals with higher type I %MHC had a greater propensity to have MUs with FRREC > FRDEREC as indicated by the slope values. Overall, the contractile properties of the muscle (MHC) could partially explain the differences in MU firing rates at de-recruitment relative to recruitment. Thus, suggesting the fatigability of the muscle influences the alterations in MU firing rates from recruitment to de-recruitment.

Accelerated exploration of multi-principal element alloys with solid solution phases.

Recent multi-principal element, high entropy alloy (HEA) development strategies vastly expand the number of candidate alloy systems, but also pose a new challenge--how to rapidly screen thousands of candidate alloy systems for targeted properties. Here we develop a new approach to rapidly assess structural metals by combining calculated phase diagrams with simple rules based on the phases present, their transformation temperatures and useful microstructures. We evaluate over 130,000 alloy systems, identifying promising compositions for more time-intensive experimental studies. We find the surprising result that solid solution alloys become less likely as the number of alloy elements increases. This contradicts the major premise of HEAs--that increased configurational entropy increases the stability of disordered solid solution phases. As the number of elements increases, the configurational entropy rises slowly while the probability of at least one pair of elements favouring formation of intermetallic compounds increases more rapidly, explaining this apparent contradiction.

Detection and attribution of urbanization effect on flood extremes using nonstationary flood-frequency models.

Urbanization is found to have an impact on high flows in a urbanized catchmentThe use of point processes is advocated for trend detection and attributionThe use of process-related covariates gives a better representation of change.

Extrolites of Wallemia sebi, a very common fungus in the built environment.

Wallemia sebi has been primarily known as a spoilage fungus of dried, salted fish and other foods that are salty or sweet. However, this fungus is also very common in house dust. The health effects of chronic exposure to mold and dampness are known to be associated with both allergens and various inflammatory compounds, including the secondary metabolites of building associated fungi and their allergens. IgE sensitization to W. sebi has been long reported from housing and occupational exposures. However, its allergens have not been described previously. Strains from food have been reported to produce a number of compounds with modest toxicity. Strains from the built environment in Canada produced a number of metabolites including the known compound walleminone and a new compound 1-benzylhexahydroimidazo [1,5-α] pyridine-3,5-dione which we call wallimidione. Based on an in silico analysis, wallimidione is likely the most toxic of the metabolites reported to date from W. sebi. We found that the primary human antigen of W. sebi is a 47 kDa excreted cellulase present in high concentrations in W. sebi arthrospores. This species is a basidiomycete and, unsurprisingly, the antigen was not found in extracts of other fungi common in the built environment, all ascomycetes.

Induction of Dectin-1 and asthma-associated signal transduction pathways in RAW 264.7 cells by a triple-helical (1, 3)-ß-D glucan, curdlan.

People living in damp buildings are typically exposed to spore and mycelial fragments of the fungi that grow on damp building materials. There is experimental evidence that this exposure to triple-helical (1, 3)-ß-D glucan and low molecular weight toxins may be associated with non-atopic asthma observed in damp and moldy buildings. However, the mechanisms underlying this response are only partially resolved. Using the pure (1, 3)-ß-D glucan, curdlan, and the murine macrophage cell line, RAW 264.7, there were two objectives of this study. The first was to determine whether signal transduction pathways activating asthma-associated cell signaling pathways were stimulated using mouse transduction Pathway Finder(®) arrays and quantitative real-time (QRT) PCR. The second objective was to evaluate the dose and temporal responses associated with transcriptional changes in asthma-associated cytokines, the signal transduction receptor gene Dectin-1, and various transcription factor genes related to the induction of asthma using customized RT-PCR-based arrays. Compared to controls, the 10(-7) M curdlan treatment induced significant changes in gene transcription predominately in the NFkB, TGF-ß, p53, JAK/STAT, P13/AKT, phospholipase C, and stress signaling pathways. The 10(-8) M curdlan treatment mainly induced NFkB and TGF-ß pathways. Compared to controls, curdlan exposures also induced significant dose- and time-dependent changes in the gene translations. We found that that curdlan as a non-allergenic potentiator modulates a network of transduction signaling pathways not only associated with TH-1, TH-2, and TH-3 cell responses including asthma potentiation, but a variety of other cell responses in RAW 264.7 cells. These results help provide mechanistic basis for some of the phenotypic changes associated with asthma that have been observed in in vitro, in vivo, and human studies and open up a hypothesis-building process that could explain the rise of non-atopic asthma associated with fungi.

Trends and causes of severity, size, and number of fires in northwestern California, USA.

Research in the last several years has indicated that fire size and frequency are on the rise in western U.S. forests. Although fire size and frequency are important, they do not necessarily scale with ecosystem effects of fire, as different ecosystems have different ecological and evolutionary relationships with fire. Our study assessed trends and patterns in fire size and frequency from 1910 to 2008 (all fires > 40 ha), and the percentage of high-severity in fires from 1987 to 2008 (all fires > 400 ha) on the four national forests of northwestern California. During 1910-2008, mean and maximum fire size and total annual area burned increased, but we found no temporal trend in the percentage of high-severity fire during 1987-2008. The time series of severity data was strongly influenced by four years with region-wide lightning events that burned huge areas at primarily low-moderate severity. Regional fire rotation reached a high of 974 years in 1984 and fell to 95 years by 2008. The percentage of high-severity fire in conifer-dominated forests was generally higher in areas dominated by smaller-diameter trees than in areas with larger-diameter trees. For Douglas-fir forests, the percentage of high-severity fire did not differ significantly between areas that re-burned and areas that only burned once (10% vs. 9%) when re-burned within 30 years. Percentage of high-severity fire decreased to 5% when intervals between first and second fires were > 30 years. In contrast, in both mixed-conifer and fir/high-elevation conifer forests, the percentage of high-severity fire was less when re-burned within 30 years compared to first-time burned (12% vs. 16% for mixed conifer; 11% vs. 19% for fir/high-elevation conifer). Additionally, the percentage of high-severity fire did not differ whether the re-burn interval was less than or greater than 30 years. Years with larger fires and greatest area burned were produced by region-wide lightning events, and characterized by less winter and spring precipitation than years dominated by smaller human-ignited fires. Overall percentage of high-severity fire was generally less in years characterized by these region-wide lightning events. Our results suggest that, under certain conditions, wildfires could be more extensively used to achieve ecological and management objectives in northwestern California.

Addressing narcissistic personality features in the context of medical care: integrating diverse perspectives to inform clinical practice.

Narcissistic personality disorder (NPD) is characterized by an unrealistic need for admiration, lack of empathy toward others, and feelings of superiority. NPD presents a unique and significant challenge in clinical practice, particularly in medical settings with limited provider contact time, as health professionals treat individuals who often require excessive admiration and have competing treatment needs. This practice review highlights real case examples across three distinct medically oriented clinical settings (inpatient and outpatient behavioral medicine and a Level I trauma center) to demonstrate the difficult and compromising situations that providers face when treating patients with general medical conditions and comorbid narcissistic personality features. The main goal of this article is to discuss the various challenges and obstacles associated with these cases in medical settings and discuss some strategies that may prove successful. A second goal is to bridge diverse conceptualizations of narcissism/NPD through the discussion of theoretical and empirical perspectives that can inform understanding of the clinical examples. Despite differing perspectives regarding the underlying motivation of narcissistic behavior, this practice review highlights that these paradigms can be integrated when sharing the same ultimate goal: to improve delivery of care across medically oriented clinical settings for patients with narcissistic features.

Sta c 3 epitopes and their application as biomarkers to detect specific IgE.

Sta c 3 was determined to be one of the major allergens from Stachybotrys chartarum sensu lato. This is among the most common fungi on wet building materials. Various cognizant authorities have found that mold and dampness are associated with exacerbation of asthma and increased upper respiratory disease. The primary amino-acid sequence of this allergen was reported, however the IgE-binding epitopes were unknown. Using SPOT-synthesis techniques (SPOTs), one main linear epitope located between V91 and G105 was discovered by Western blot with atopic human sera. This was confirmed by inhibition ELISA assays with both synthesized epitopes and natural Sta c 3. Alanine scanning also revealed R100 and K101 are the critical amino acids for IgE binding. Using the binding-enhanced and alanine substituted epitopes as potential biomarkers, a reverse ELISA method was developed to detect the Sta c 3 sIgE in atopic human sera. This method employed streptavidin-biotinylated mutated epitopes as the capture matrix, probing the possibility of using small peptides for allergen/species specific IgE assays.

Unplanned excision of soft tissue sarcoma results in increased rates of local recurrence despite full further oncological treatment.

BACKGROUND: Unplanned excision of soft tissue sarcoma (STS) accounts for up to 40% of all initial operations for STS and is undertaken when the mass is presumed to be benign. The effect this has on outcome has never been fully established. METHODS: Patients with extremity or trunk STS between 2001 and 2005 who were treated by an initial inadvertent operation and then referred immediately to our unit were identified. Outcomes were compared with a control group of patients with STS who were stage-matched and had been treated conventionally by core biopsy and definitive surgery. Endpoints were local recurrence, distant metastases and sarcoma-specific survival. RESULTS: 134 patients who had undergone unplanned excision of STS were identified. One hundred twenty-one underwent further re-excision, and 51 (48%) of these patients had residual tumour identified after surgical re-excision. Two hundred nine stage-matched controls were identified who were treated conventionally. Median follow-up was 51.6 months. Local recurrence rates were considerably higher in the study group (23.8 vs. 11%, p = 0.0016), despite the control group having more stage 3 tumours. When the tumours were matched by stage, an increase in local recurrence was seen across all stages but was most pronounced for stage 3 tumours (37.5 vs. 14.2%, p = 0.005). Metastasis-free and sarcoma-specific survival were also significantly increased for stage 3 tumours. CONCLUSION: Unplanned initial excision of extremity soft tissue sarcoma may compromise long-term local control of extremity STS despite full further oncological management.

Characterization of the 41kDa allergen Asp v 13, a subtilisin-like serine protease from Aspergillus versicolor.

Aspergillus versicolor is common on moldy building materials. Asp v 13, the principal allergen is produced by strains collected from across Canada. In this paper, we report a 1833bp Asp v 13 open reading frame predicted to encode a protein of 403 amino acids in length with three introns. A BLAST search of Asp v 13, a phylogenic tree calculation and alignment with its homologous proteins from other species indicated that Asp v 13 is a secretory, subtilisin-like serine protease widely distributed in Aspergillus species. His-tagged Asp v 13 was over-expressed in Escherichia coli and purified using Ni-NTA columns with a yield of 1mg/L. Based on immuno binding assay of recombinant protein both antibodies developed against the natural protein, and human sera IgE, the recombinant protein was similar to the natural form. Six IgE- and seven IgG-binding epitopes were also identified with selected human sera along the entire amino acid sequence of Asp v 13. Most residues binding these epitopes are exposed on the surface and correspond to charged regions of the molecule.

Resistance to recombinant stem rust race TPPKC in hard red spring wheat.

The wheat (Triticum aestivum L.) stem rust (Puccinia graminis Pers.:Pers. f.sp. tritici Eriks. and Henn.) resistance gene SrWld1 conditions resistance to all North American stem rust races and is an important gene in hard red spring (HRS) wheat cultivars. A sexually recombined race having virulence to SrWld1 was isolated in the 1980s. Our objective was to determine the genetics of resistance to the race. The recombinant race was tested with the set of stem rust differentials and with a set of 36 HRS and 6 durum cultivars. Chromosomal location studies in cultivars Len, Coteau, and Stoa were completed using aneuploid analysis, molecular markers, and allelism tests. Stem rust differential tests coded the race as TPPKC, indicating it differed from TPMKC by having added virulence on Sr30 as well as SrWld1. Genes effective against TPPKC were Sr6, Sr9a, Sr9b, Sr13, Sr24, Sr31, and Sr38. Genetic studies of resistance to TPPKC indicated that Len, Coteau, and Stoa likely carried Sr9b, that Coteau and Stoa carried Sr6, and Stoa carried Sr24. Tests of HRS and durum cultivars indicated that five HRS and one durum cultivar were susceptible to TPPKC. Susceptible HRS cultivars were postulated to have SrWld1 as their major stem rust resistance gene. Divide, the susceptible durum cultivar, was postulated to lack Sr13. We concluded that although TPPKC does not constitute a threat similar to TTKSK and its variants, some cultivars would be lost from production if TPPKC became established in the field.

Effects of low molecular weight fungal compounds on inflammatory gene transcription and expression in mouse alveolar macrophages.

The inflammatory potential and molecular mechanisms underscoring inflammatory responses of lung cells to compounds from fungi that grow on damp building materials is poorly understood in vitro. In this study we evaluated the effect of pure fungal compounds on potentiating acute inflammatory response in primary mouse alveolar macrophages (AMs) and tested the hypothesis that AM responses to low molecular weight fungal compounds exhibit temporal and compound specificity that mimic that observed in the whole lung. Transcriptional responses of 13 inflammation/respiratory burst-associated genes (KC=Cxcl1, Cxcl2, Cxcl5, Cxcl10, Ccl3, Ccl112, Ccl20, IL-1ß, Il-6, ifi27 Tnfα, iNOS and Blvrb) were evaluated in mouse AMs exposed to a 1ml (10(-8)mol) dose of either pure atranone C, brevianimide, cladosporin, curdlan, LPS, neoechinulin A & B, sterigmatocystin or TMC-120A for 2h, 4h and 12h PE using customized reverse transcription (RT)-PCR based arrays. Multianalyte ELISA was used to measure expression of 6 pro-inflammatory cytokines common to the transcriptional assays (Cxcl1, Cxcl10, Ccl3, IL1ß, Ifn-λ and Tnf-α) to determine whether gene expression corresponded to the transcription data. Compared to controls, all of these compounds induced significant (≥2.5-fold or ≤-2.5-fold change at p≤0.05) time- and compound-specific transcriptional gene alterations in treatment AMs. The highest number of transcribed genes were in LPS treatment AMs at 12h PE (12/13) followed by neoechinulin B at 4h PE (11/13). Highest fold change values (>30) were associated with KC, Cxcl2, Cxcl5 and IL1ß genes in cells exposed to LPS. Compound exposures also induced significant (p≤0.05) time- and compound-specific pro-inflammatory responses manifest as differentially elevated Cxcl1, Cxcl10, Ccl3, Ifn-λ and Tnf-α concentrations in culture supernatant of treatment AMs. Dissimilarity in transcriptional responses in AMs and our in vivo model of lung disease is likely attributable to whole lung vs. isolated cell responsive and dose differences between the two studies. The results not only indicate that low molecular weight compounds from fungi that grow in damp built environments are potently pro-inflammatory in vitro, it further highlights the important role AMs play in innate lung defence, and against exposure to low molecular weight fungal compounds. These observations further support our position that exposure to low molecular weight compounds from indoor-associated fungi may provoke some of the inflammatory health effects reported from humans in damp building environments. They also open up a hypothesis building process that could explain the rise of non-atopic asthma associated with fungi.

Detecting 2009 pandemic influenza A (H1N1) virus infection: availability of diagnostic testing led to rapid pandemic response.

Diagnostic tests for detecting emerging influenza virus strains with pandemic potential are critical for directing global influenza prevention and control activities. In 2008, the Centers for Disease Control and Prevention received US Food and Drug Administration approval for a highly sensitive influenza polymerase chain reaction (PCR) assay. Devices were deployed to public health laboratories in the United States and globally. Within 2 weeks of the first recognition of 2009 pandemic influenza H1N1, the Centers for Disease Control and Prevention developed and began distributing a new approved pandemic influenza H1N1 PCR assay, which used the previously deployed device platform to meet a >8-fold increase in specimen submissions. Rapid antigen tests were widely used by clinicians at the point of care; however, test sensitivity was low (40%-69%). Many clinical laboratories developed their own pandemic influenza H1N1 PCR assays to meet clinician demand. Future planning efforts should identify ways to improve availability of reliable testing to manage patient care and approaches for optimal use of molecular testing for detecting and controlling emerging influenza virus strains.