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1.
Curr Opin Biotechnol ; 67: 80-87, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33508634

RESUMO

To realize a circular, carbon-neutral economy, it will become important to utilize the greenhouse gas CO2 as a sustainable carbon source. Carboxylases, the enzymes that capture and convert gaseous CO2 are the prime candidates to pave the way towards realizing this vision of a CO2-based bio-economy. In the last couple of years, the interest in using and engineering carboxylases has been steadily growing. Here, we discuss how basic research on the mechanism of CO2 binding and activation by carboxylases opened the way to develop new-to-nature CO2-fixing enzymes that found application in the development of synthetic CO2-fixation pathways and their further realization in vitro and in vivo. These pioneering efforts in the field pave the way to realize a diverse CO2-fixation biochemistry that can find application in biocatalysis, biotechnology, and artificial photosynthesis.


Assuntos
Dióxido de Carbono , Fotossíntese , Biotecnologia , Carbono , Ciclo do Carbono
2.
Science ; 368(6491): 649-654, 2020 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-32381722

RESUMO

Nature integrates complex biosynthetic and energy-converting tasks within compartments such as chloroplasts and mitochondria. Chloroplasts convert light into chemical energy, driving carbon dioxide fixation. We used microfluidics to develop a chloroplast mimic by encapsulating and operating photosynthetic membranes in cell-sized droplets. These droplets can be energized by light to power enzymes or enzyme cascades and analyzed for their catalytic properties in multiplex and real time. We demonstrate how these microdroplets can be programmed and controlled by adjusting internal compositions and by using light as an external trigger. We showcase the capability of our platform by integrating the crotonyl-coenzyme A (CoA)/ethylmalonyl-CoA/hydroxybutyryl-CoA (CETCH) cycle, a synthetic network for carbon dioxide conversion, to create an artificial photosynthetic system that interfaces the natural and the synthetic biological worlds.


Assuntos
Dióxido de Carbono/metabolismo , Cloroplastos/metabolismo , Cloroplastos/efeitos da radiação , Acil Coenzima A , Biocatálise , Biomimética , Ciclo do Carbono , Luz , Fotossíntese/efeitos da radiação , Spinacia oleracea
3.
Plant Cell ; 30(2): 447-465, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29437989

RESUMO

Photosynthesis occurs in the thylakoid membrane, where the predominant lipid is monogalactosyldiacylglycerol (MGDG). As environmental conditions change, photosynthetic membranes have to adjust. In this study, we used a loss-of-function Chlamydomonas reinhardtii mutant deficient in the MGDG-specific lipase PGD1 (PLASTID GALACTOGLYCEROLIPID DEGRADATION1) to investigate the link between MGDG turnover, chloroplast ultrastructure, and the production of reactive oxygen species (ROS) in response to different adverse environmental conditions. The pgd1 mutant showed altered MGDG abundance and acyl composition and altered abundance of photosynthesis complexes, with an increased PSII/PSI ratio. Transmission electron microscopy showed hyperstacking of the thylakoid grana in the pgd1 mutant. The mutant also exhibited increased ROS production during N deprivation and high light exposure. Supplementation with bicarbonate or treatment with the photosynthetic electron transport blocker DCMU protected the cells against oxidative stress in the light and reverted chlorosis of pgd1 cells during N deprivation. Furthermore, exposure to stress conditions such as cold and high osmolarity induced the expression of PGD1, and loss of PGD1 in the mutant led to increased ROS production and inhibited cell growth. These findings suggest that PGD1 plays essential roles in maintaining appropriate thylakoid membrane composition and structure, thereby affecting growth and stress tolerance when cells are challenged under adverse conditions.


Assuntos
Proteínas de Algas/metabolismo , Chlamydomonas reinhardtii/enzimologia , Galactolipídeos/metabolismo , Lipase/metabolismo , Tilacoides/metabolismo , Proteínas de Algas/genética , Chlamydomonas reinhardtii/genética , Chlamydomonas reinhardtii/fisiologia , Cloroplastos/metabolismo , Transporte de Elétrons , Meio Ambiente , Lipase/genética , Fotossíntese , Estresse Fisiológico
4.
Plant Cell ; 26(11): 4499-518, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25381350

RESUMO

Chlamydomonas reinhardtii insertion mutants disrupted for genes encoding acetate kinases (EC 2.7.2.1) (ACK1 and ACK2) and a phosphate acetyltransferase (EC 2.3.1.8) (PAT2, but not PAT1) were isolated to characterize fermentative acetate production. ACK1 and PAT2 were localized to chloroplasts, while ACK2 and PAT1 were shown to be in mitochondria. Characterization of the mutants showed that PAT2 and ACK1 activity in chloroplasts plays a dominant role (relative to ACK2 and PAT1 in mitochondria) in producing acetate under dark, anoxic conditions and, surprisingly, also suggested that Chlamydomonas has other pathways that generate acetate in the absence of ACK activity. We identified a number of proteins associated with alternative pathways for acetate production that are encoded on the Chlamydomonas genome. Furthermore, we observed that only modest alterations in the accumulation of fermentative products occurred in the ack1, ack2, and ack1 ack2 mutants, which contrasts with the substantial metabolite alterations described in strains devoid of other key fermentation enzymes.


Assuntos
Acetato Quinase/metabolismo , Acetatos/metabolismo , Chlamydomonas reinhardtii/enzimologia , Cloroplastos/metabolismo , Fosfato Acetiltransferase/metabolismo , Acetato Quinase/genética , Proteínas de Algas/genética , Proteínas de Algas/metabolismo , Chlamydomonas reinhardtii/genética , Fermentação , Mitocôndrias/metabolismo , Mutagênese Insercional , Fosfato Acetiltransferase/genética
5.
Proc Natl Acad Sci U S A ; 111(37): 13313-8, 2014 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-25157174

RESUMO

Biofilm-protected microbial infections in skin are a serious health risk that remains to be adequately addressed. The lack of progress in developing effective treatment strategies is largely due to the transport barriers posed by the stratum corneum of the skin and the biofilm. In this work, we report on the use of Ionic Liquids (ILs) for biofilm disruption and enhanced antibiotic delivery across skin layers. We outline the syntheses of ILs, analysis of relevant physicochemical properties, and subsequent neutralization effects on two biofilm-forming pathogens: Pseudomonas aeruginosa and Salmonella enterica. Further, the ILs were also examined for cytotoxicity, skin irritation, delivery of antibiotics through the skin, and treatment of biofilms in a wound model. Of the materials examined, choline-geranate emerged as a multipurpose IL with excellent antimicrobial activity, minimal toxicity to epithelial cells as well as skin, and effective permeation enhancement for drug delivery. Specifically, choline-geranate was comparable with, or more effective than, bleach treatment against established biofilms of S. enterica and P. aeruginosa, respectively. In addition, choline-geranate increased delivery of cefadroxil, an antibiotic, by >16-fold into the deep tissue layers of the skin without inducing skin irritation. The in vivo efficacy of choline-geranate was validated using a biofilm-infected wound model (>95% bacterial death after 2-h treatment). This work establishes the use of ILs for simultaneous enhancement of topical drug delivery and antibiotic activity.


Assuntos
Sistemas de Liberação de Medicamentos , Líquidos Iônicos/farmacologia , Pseudomonas aeruginosa/fisiologia , Salmonella enterica/fisiologia , Administração Cutânea , Biofilmes/efeitos dos fármacos , Morte Celular/efeitos dos fármacos , Células Epiteliais/citologia , Células Epiteliais/efeitos dos fármacos , Humanos , Irritantes/toxicidade , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Reprodutibilidade dos Testes , Salmonella enterica/efeitos dos fármacos , Pele/efeitos dos fármacos , Pele Artificial/microbiologia , Espectroscopia de Infravermelho com Transformada de Fourier
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