Course on Breeding, Handling, and Experimental Procedures of Zebrafish (Danio rerio): Training, Refinement, Paradigm Shift, and Dissemination of Knowledge from Brazil to the World.

Currently, in Brazil, all researchers involved in animal experimentation must undergo training in laboratory animal science to stay updated on biology, methodology, ethics, and legal considerations related to the use of animals. The training program presented in this study not only aims to fulfill a legal obligation but also intends to train students and professionals to effectively care for their biomodels. It seeks to help them understand the importance of this care, both for the welfare of the animals and for the results of their projects. In total, 58 participants were present at the event (pre-event and full-time course). These participants consisted students and professionals from 11 institutions and 5 different countries. These numbers demonstrate the successful attainment of the desired capillarity in the scientific community and the posterior dissemination of knowledge. Through this course, it was possible to train the participants and raise their awareness about the importance of applying scientific knowledge in their daily practices to maintain the animals, ensuring the welfare of the models and refining the research. Finally, the program presented in this study, as well as the strategies adopted, can serve as a model for other institutions aiming to achieve similar results.

Glyphosate is Harmful to Early Life Stages of the Viviparous Fish Jenynsia Multidentata: Biochemical and Locomotor Effects.

Glyphosate is the most widely used herbicide worldwide due to its efficacy in weed control in agriculture. This herbicide has been consistently detected in the aquatic environment, causing harmful consequences to nontarget organisms residing in agricultural regions. In this study, we assessed the effects of environmentally relevant concentrations of glyphosate (30-100 µg/L) on the early life stages of the viviparous fish Jenynsia multidentata through biochemical and locomotor endpoints. At 96 h of exposure, 30 and 65 µg/L glyphosate caused an increase in acetylcholinesterase (AChE) activity, and 65 µg/L glyphosate also augmented the levels of lipid peroxidation. Glyphosate at 100 µg/L did not alter the activity of acetylcholinesterase or the levels of lipid peroxidation, but it stimulated the activity of the cellular detoxification enzyme glutathione S-transferase. In addition, all concentrations affected the swimming of the fish. Under light conditions, glyphosate caused hypolocomotion at all concentrations tested, whereas under dark conditions, this was observed at 30 and 100 µg/L. Hyperlocomotion was observed at 65 µg/L glyphosate. These findings are alarming for the health of fish, such as J. multidentata that inhabit streams that pass through agricultural areas, especially for the early life stages of these fish. Research studying the effects of pollutants on native species is relevant to improve regulation that protects aquatic ecosystems.

Effects of the herbicide glyphosate on fish from embryos to adults: a review addressing behavior patterns and mechanisms behind them.

The use of agrochemicals has grown in recent years following the increase in agricultural productivity, to eliminate weeds that can compromise crop yields. The intensive use of these products combined with the lack of treatment of agricultural wastewater is causing contamination of the natural environments, especially the aquatics. Glyphosate [N-(phosphonomethyl) glycine] is the most commonly used herbicide in agriculture worldwide. Studies have shown that this compound is toxic to a variety of fish species at the concentrations of environmental relevance. Glyphosate-based herbicides can affect fish biochemical, physiological, endocrine, and behavioral pathways. Changes in behaviors such as foraging, escaping from predators, and courtship can compromise the survival of species and even communities. The behavior patterns of fish has been shown to be a sensitive tool for risk assessment. In this sense, this review summarizes and discusses the toxic effects of glyphosate and its formulations on the behavior of fish in different life stages. Additionally, behavioral impairments were associated with other negative effects of glyphosate such as energy imbalance, stress responses, AChE inhibition, and physiological and endocrine disturbances, which are evidenced and described in the literature. Graphical abstract.

Zebrafish (Danio rerio) ability to activate ABCC transporters after exposure to glyphosate and its formulation Roundup Transorb®.

The emergent demand for food production has increased the widespread use of pesticides, especially glyphosate-based herbicides as they can protect different types of crops, especially transgenic ones. Molecules of glyphosate have been found in water bodies around the world, and its presence can cause negative effects on non-target organisms, such as fish. Glyphosate toxicity appears to be systemic in fish but does not affect their organs equally. Also, its formulations can be more toxic than pure glyphosate. In this sense, we investigated if these variations in toxicity could be related to ATP binding cassette subfamily C (ABCC) transporters and the cellular detoxification capacity, following exposure to herbicides. Thus, adults of Danio rerio were exposed (24 and 96 h) to glyphosate and Roundup Transorb® (RT) at an environmental concentration of 0.1 mg/L, and the activity of ABCC proteins and gene expression of five isoforms of ABCC were analyzed. Glyphosate and RT exposure increased ABCC protein activity and gene expression up to 3-fold when compared to controls, indicating the activation of detoxification mechanisms. Only in the brain of D. rerio, the exposure to RT did not stimulate the activity of ABCC proteins, neither the expression of genes abcc1 and abcc4 that responded to the exposure to pure glyphosate. These results may suggest that the brain is more sensitive to RT than the other target-tissues since the mechanism of detoxification via ABCC transporters were not activated in this tissue as it was in the other.

Disfunção do sistema estomatognático e sua relação com a síndrome fibromiálgica / Stomatognathic system dysfunction and its relation with fibromyalgia syndrome

A síndrome fibromiálgica (SFM) e a desordem temporomandibular (DTM) têm como principal característica a presença de dor crônica, podendo compartilhar de aspectos comuns na fisiopatologia e nas características clínicas. A associação entre DTM e SFM tem sido relatada por meio de estudos clínicos. Objetivo: avaliar a redução da sintomatologia dolorosa de pacientes com fibromialgia do projeto de pesquisa "Avaliação de portadores de desordens temporomandibulares" da Faculdade de Odontologia da Universidade Federal de Goiás (UFG), por meio do tratamento da DTM. Material e métodos: a amostra foi composta por 17 pacientes, de idades entre 21 e 65 anos e apenas um era do gênero masculino. Resultados: 88,23% dos casos avaliados tiveram sucesso no tratamento, com a redução da sintomatologia dolorosa. Conclusões: as condições de harmonia e equilíbrio funcional do sistema estomatognático são capazes de devolver o bem-estar de pacientes diagnosticados com a síndrome fibromiálgica.

Fibromyalgia syndrome (FM) and temporomandibular joint disorder (TMD) has as main feature the presence of chronic pain and may share common aspects, in the pathophysiology and clinical features. The association between TMD and FM has been reported through clinical studies. Objective: Evaluate the reduction of painful symptoms of patients with Fibromyalgia Research project: "Evaluation of patients with temporomandibular disorders" that happened in dentistry school of the Federal University of Goiás, with the treatment of TMD. Material and methods: the sample was made of 17 patients, aged 21 to 65 years and only one was male. Results: 82.35% of evaluated cases had success with the treatment with reduction the painful symptoms. Conclusions: the conditions of harmony and functional balance of the Stomatognathic system are able to return the welfare of patients diagnosed with Fibromyalgia syndrome.