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1.
Braz. j. biol ; 83: 1-5, 2023. ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1468893

RESUMO

Morphophysiological species researches are fundamental, and diagnostic imaging is an excellent technique, already used in wild animals, with great application, not invasive and provide real-time information of each body. Amazonian manatees are on the list of endangered animals classified in the vulnerable category and knowledge of the normal pattern of ultrasound anatomy of organs and tissues is important for the maintenance and well-being of captive specimens contributing to reintroduction actions. The objective of the study was to standardize the examination technique and describe the ultrasound findings of the liver, gallbladder, stomach, urinary bladder and the subcutaneous tissue of the abdominal region in Trichechus inunguis, in order to contribute with the anatomical and sonographic knowledge and assist in the diagnosis and prognosis diseases. The study used 18 animals to describe the normal sonographic anatomy in the abdominal cavity of the Amazonian manatee. During abdominal scan, it was possible to visualize the features of the liver, gallbladder, stomach, urinary bladder obtained satisfactory results in this study. Therefore, other structures were not primarily identified by the reduced time, lots of fat and gases in intestines of animals.


Pesquisas morfofisiológicas em espécies selvagens são fundamentais, e o diagnóstico por imagem é uma excelente técnica, já usada e com grande aplicação, não invasiva e que fornece informações em tempo real de cada órgão. Peixes-boi-amazônico encontram-se na lista de animais ameaçados de extinção classificados na categoria vulnerável e o conhecimento do padrão normal da anatomia ultrassonográfica de órgãos e tecidos é importante para a manutenção e bem-estar de espécimes em cativeiro contribuindo para ações de reintrodução. O objetivo deste estudo foi padronizar a técnica de exame e descrever os achados ultrassonográficos do fígado, vesícula biliar, estômago, vesícula urinária e o tecido subcutâneo da região abdominal em Trichechus inunguis, de modo a contribuir com o conhecimento anátomo-sonográfico e auxiliar no diagnóstico e prognóstico de doenças. O estudo utilizou 18 animais para descrever a anatomia ultrassonográfica normal na cavidade abdominal de peixe-boi amazônico. Durante a varredura abdominal foi possível visualizar as características dos órgãos obtendo resultados satisfatórios neste estudo, concluindo ser uma técnica eficiente para avaliação de determinados órgãos abdominais em peixe-boi amazônico. Entretanto, outras estruturas não foram identificadas principalmente pelo tempo reduzido, muita gordura e gases nos intestinos dos animais.


Assuntos
Animais , Anatomia Transversal , Bexiga Urinária/anatomia & histologia , Estômago/anatomia & histologia , Fígado/anatomia & histologia , Tela Subcutânea/anatomia & histologia , Trichechus , Ultrassonografia/métodos , Ultrassonografia/veterinária , Vesícula Biliar/anatomia & histologia
2.
Braz. j. biol ; 832023.
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469109

RESUMO

Abstract Morphophysiological species researches are fundamental, and diagnostic imaging is an excellent technique, already used in wild animals, with great application, not invasive and provide real-time information of each body. Amazonian manatees are on the list of endangered animals classified in the vulnerable category and knowledge of the normal pattern of ultrasound anatomy of organs and tissues is important for the maintenance and well-being of captive specimens contributing to reintroduction actions. The objective of the study was to standardize the examination technique and describe the ultrasound findings of the liver, gallbladder, stomach, urinary bladder and the subcutaneous tissue of the abdominal region in Trichechus inunguis, in order to contribute with the anatomical and sonographic knowledge and assist in the diagnosis and prognosis diseases. The study used 18 animals to describe the normal sonographic anatomy in the abdominal cavity of the Amazonian manatee. During abdominal scan, it was possible to visualize the features of the liver, gallbladder, stomach, urinary bladder obtained satisfactory results in this study. Therefore, other structures were not primarily identified by the reduced time, lots of fat and gases in intestines of animals.


Resumo Pesquisas morfofisiológicas em espécies selvagens são fundamentais, e o diagnóstico por imagem é uma excelente técnica, já usada e com grande aplicação, não invasiva e que fornece informações em tempo real de cada órgão. Peixes-boi-amazônico encontram-se na lista de animais ameaçados de extinção classificados na categoria vulnerável e o conhecimento do padrão normal da anatomia ultrassonográfica de órgãos e tecidos é importante para a manutenção e bem-estar de espécimes em cativeiro contribuindo para ações de reintrodução. O objetivo deste estudo foi padronizar a técnica de exame e descrever os achados ultrassonográficos do fígado, vesícula biliar, estômago, vesícula urinária e o tecido subcutâneo da região abdominal em Trichechus inunguis, de modo a contribuir com o conhecimento anátomo-sonográfico e auxiliar no diagnóstico e prognóstico de doenças. O estudo utilizou 18 animais para descrever a anatomia ultrassonográfica normal na cavidade abdominal de peixe-boi amazônico. Durante a varredura abdominal foi possível visualizar as características dos órgãos obtendo resultados satisfatórios neste estudo, concluindo ser uma técnica eficiente para avaliação de determinados órgãos abdominais em peixe-boi amazônico. Entretanto, outras estruturas não foram identificadas principalmente pelo tempo reduzido, muita gordura e gases nos intestinos dos animais

3.
Braz J Biol ; 83: e247609, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34468528

RESUMO

Morphophysiological species researches are fundamental, and diagnostic imaging is an excellent technique, already used in wild animals, with great application, not invasive and provide real-time information of each body. Amazonian manatees are on the list of endangered animals classified in the vulnerable category and knowledge of the normal pattern of ultrasound anatomy of organs and tissues is important for the maintenance and well-being of captive specimens contributing to reintroduction actions. The objective of the study was to standardize the examination technique and describe the ultrasound findings of the liver, gallbladder, stomach, urinary bladder and the subcutaneous tissue of the abdominal region in Trichechus inunguis, in order to contribute with the anatomical and sonographic knowledge and assist in the diagnosis and prognosis diseases. The study used 18 animals to describe the normal sonographic anatomy in the abdominal cavity of the Amazonian manatee. During abdominal scan, it was possible to visualize the features of the liver, gallbladder, stomach, urinary bladder obtained satisfactory results in this study. Therefore, other structures were not primarily identified by the reduced time, lots of fat and gases in intestines of animals.


Assuntos
Trichechus inunguis , Animais
4.
J Dairy Sci ; 101(11): 10116-10125, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30146281

RESUMO

The emergence of antimicrobial resistance to commonly used antibiotics has necessitated the development of new antimicrobial products. Crude extracts produced by actinomycetes contain antimicrobial metabolites that can inhibit bacterial growth. The objective of our study was to evaluate the antimicrobial activity of crude extracts (Caat1-54 and CaatP5-8) produced by actinomycetes against isolates of Staphylococcus aureus, Staphylococcus chromogenes, Streptococcus dysgalactiae, and Streptococcus uberis, which were obtained from the milk of cows affected by mastitis in 23 dairy herds. Twenty isolates of each bacterial species were used to define minimum inhibitory concentrations (MIC) of both crude extracts and ceftiofur (positive control). The MIC50 and MIC90 were defined at the concentration required to inhibit the growth of 50 and 90% of bacterial isolates tested, respectively. The MIC results were evaluated by survival analysis. Staphylococcus aureus isolates presented MIC90 of Caat 1-54 ≥6.25 µg/mL, ceftiofur ≥12.5 µg/mL, and Caat P5-8 ≥100 µg/mL. Streptococcus uberis presented MIC90 of ceftiofur ≥0.39 µg/mL, Caat 1-54 ≥50 µg/mL, and Caat P5-8 ≥100 µg/mL. Staphylococcus chromogenes isolated from subclinical mastitis presented MIC90 of Caat 1-54 ≥0.78 µg/mL and ceftiofur and Caat P5-8 of ≥6.25 and ≥100 µg/mL, respectively. Streptococcus dysgalactiae isolated from clinical mastitis presented similar MIC90 values between antimicrobials tested (ceftiofur, Caat 1-54, and Caat P-58), but these values (≥100 µg/mL) were higher than the values obtained from other pathogens evaluated in the present study. Our results indicate that Caat 1-54 and Caat P5-8 crude extracts present in vitro antimicrobial activity against isolates of Staph. aureus, Staph. chromogenes, Strep. dysgalactiae, and Strep. uberis isolated from clinical and subclinical mastitis.


Assuntos
Actinobacteria/química , Anti-Infecciosos/farmacologia , Misturas Complexas/farmacologia , Mastite Bovina/tratamento farmacológico , Infecções Estafilocócicas/veterinária , Infecções Estreptocócicas/veterinária , Animais , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Bovinos , Cefalosporinas/química , Cefalosporinas/isolamento & purificação , Cefalosporinas/farmacologia , Misturas Complexas/química , Misturas Complexas/isolamento & purificação , Feminino , Mastite Bovina/microbiologia , Testes de Sensibilidade Microbiana/veterinária , Leite/microbiologia , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/microbiologia , Staphylococcus/efeitos dos fármacos , Infecções Estreptocócicas/tratamento farmacológico , Infecções Estreptocócicas/microbiologia , Streptococcus/efeitos dos fármacos
5.
Int J Biol Macromol ; 108: 291-299, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29191425

RESUMO

The present study describes the one-step purification and biochemical characterization of an endo-1,4-ß-xylanase from Aspergillus tamarii Kita. Extracellular xylanase was purified to homogeneity 7.43-fold through CM-cellulose. Enzyme molecular weight and pI were estimated to be 19.5kDa and 8.5, respectively. The highest activity of the xylanase was obtained at 60°C and it was active over a broad pH range (4.0-9.0), with maximal activity at pH 5.5. The enzyme was thermostable at 50°C, retaining more than 70% of its initial activity for 480min. The K0.5 and Vmax values on beechwood xylan were 8.13mg/mL and 1,330.20µmol/min/mg of protein, respectively. The ions Ba2+ and Ni2+, and the compounds ß-mercaptoethanol and DTT enhanced xylanase activity, while the heavy metals (Co2+, Cu2+, Hg+, Pb2+ and Zn2+) strongly inhibited the enzyme, at 5mM. Enzymatic hydrolysis of xylooligosaccharides monitored in real-time by mass spectrometer showed that the shortest xylooligosaccharide more efficiently hydrolyzed by A. tamarii Kita xylanase corresponded to xylopentaose. In agreement, HPLC analyzes did not detect xylopentaose among the hydrolysis products of xylan. Therefore, this novel GH11 endo-xylanase displays a series of physicochemical properties favorable to its application in the food, feed, pharmaceutical and paper industries.


Assuntos
Aspergillus/enzimologia , Xilosidases/química , Cromatografia , Cromatografia Líquida de Alta Pressão , Ativação Enzimática , Estabilidade Enzimática , Glucuronatos , Hidrólise , Cinética , Espectrometria de Massas , Modelos Moleculares , Peso Molecular , Oligossacarídeos , Conformação Proteica , Proteínas Recombinantes , Especificidade por Substrato , Xilosidases/isolamento & purificação
6.
J Thromb Haemost ; 8(11): 2530-41, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20723025

RESUMO

BACKGROUND: Platelet activation by collagen depends on signals transduced by the glycoprotein (GP)VI-Fc receptor (FcR)γ-chain collagen receptor complex, which involves recruitment of phosphatidylinositol 3-kinase (PI3K) to phosphorylated tyrosines in the linker for activation of T cells (LAT). An interaction between the p85 regulatory subunit of PI3K and the scaffolding molecule Grb-2-associated binding protein-1 (Gab1), which is regulated by binding of the Src homology 2 domain-containing protein tyrosine phosphatase-2 (SHP-2) to Gab1, has been shown in other cell types to sustain PI3K activity to elicit cellular responses. Platelet endothelial cell adhesion molecule-1 (PECAM-1) functions as a negative regulator of platelet reactivity and thrombosis, at least in part by inhibiting GPVI-FcRγ-chain signaling via recruitment of SHP-2 to phosphorylated immunoreceptor tyrosine-based inhibitory motifs in PECAM-1. OBJECTIVE: To investigate the possibility that PECAM-1 regulates the formation of the Gab1-p85 signaling complexes, and the potential effect of such interactions on GPVI-mediated platelet activation in platelets. METHODS: The ability of PECAM-1 signaling to modulate the LAT signalosome was investigated with immunoblotting assays on human platelets and knockout mouse platelets. RESULTS: PECAM-1-associated SHP-2 in collagen-stimulated platelets binds to p85, which results in diminished levels of association with both Gab1 and LAT and reduced collagen-stimulated PI3K signaling. We therefore propose that PECAM-1-mediated inhibition of GPVI-dependent platelet responses result, at least in part, from recruitment of SHP-2-p85 complexes to tyrosine-phosphorylated PECAM-1, which diminishes the association of PI3K with activatory signaling molecules, such as Gab1 and LAT.


Assuntos
Plaquetas/metabolismo , Colágeno/metabolismo , Proteína Adaptadora GRB2/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Molécula-1 de Adesão Celular Endotelial a Plaquetas/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Plaquetas/citologia , Classe Ia de Fosfatidilinositol 3-Quinase/metabolismo , Humanos , Proteínas de Membrana/metabolismo , Camundongos , Fosfoproteínas/metabolismo , Fosforilação , Ativação Plaquetária , Glicoproteínas da Membrana de Plaquetas/metabolismo , Proteína Tirosina Fosfatase não Receptora Tipo 11/metabolismo , Transdução de Sinais , Tirosina/química
7.
G Chir ; 31(3): 80-2, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20426916

RESUMO

Radiographic findings of free air in the peritoneal cavity secondary to perforation of a acutely inflamed appendix are extremely rare. It accounts for about 0-7% of all patients with pneumoperitoneum. We report on a 58-years-old Brazilian Amazon woman presenting a 1- week history of abdominal pain, tenderness and distension associated with asthenia and without passage of stool or gas. Abdominal percussion revealed a tympanic sound located on the right hypocondrium. Plain chest radiography revealed a large amount of free air beneath the right leaf of the diaphram. The patient was taken immediately to the operation room and, during surgery, a gangrenous appendix with an apex perforation was verified. Appendectomy was performed as routinely. The patient evolved with pneumonia and septic shock that responded well to intravenous antibiotics and vasoactive drugs. She was discharged to home on the twenty-first post-operative day in good clinical conditions. This case highlights that perforated acute appendicitis is rarely associated with pneumoperitoneum, but it must be considered in the differential diagnosis of patients presenting right abdominal pain and free intraperitoneal air.


Assuntos
Apendicectomia , Apendicite/complicações , Apendicite/cirurgia , Pneumoperitônio/etiologia , Pneumoperitônio/cirurgia , Apendicite/diagnóstico , Brasil , Diagnóstico Diferencial , Feminino , Humanos , Pessoa de Meia-Idade , Pneumoperitônio/diagnóstico , Resultado do Tratamento
8.
J Thromb Haemost ; 8(3): 577-87, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20040043

RESUMO

BACKGROUND: Peroxisome proliferator-activated receptor-(gamma) (PPAR(gamma)) is expressed in human platelets although in the absence of genomic regulation in these cells, its functions are unclear. OBJECTIVE: In the present study, we aimed to demonstrate the ability of PPAR(gamma) ligands to modulate collagen-stimulated platelet function and suppress activation of the glycoprotein VI (GPVI) signaling pathway. METHODS: Washed platelets were stimulated with PPAR(gamma) ligands in the presence and absence of PPAR(gamma) antagonist GW9662 and collagen-induced aggregation was measured using optical aggregometry. Calcium levels were measured by spectrofluorimetry in Fura-2AM-loaded platelets and tyrosine phosphorylation levels of receptor-proximal components of the GPVI signaling pathway were measured using immunoblot analysis. The role of PPAR(gamma) agonists in thrombus formation was assessed using an in vitro model of thrombus formation under arterial flow conditions. RESULTS: PPAR(gamma) ligands inhibited collagen-stimulated platelet aggregation that was accompanied by a reduction in intracellular calcium mobilization and P-selectin exposure. PPAR(gamma) ligands inhibited thrombus formation under arterial flow conditions. The incorporation of GW9662 reversed the inhibitory actions of PPAR(gamma) agonists, implicating PPAR(gamma) in the effects observed. Furthermore, PPAR(gamma) ligands were found to inhibit tyrosine phosphorylation levels of multiple components of the GPVI signaling pathway. PPAR(gamma) was found to associate with Syk and LAT after platelet activation. This association was prevented by PPAR(gamma) agonists, indicating a potential mechanism for PPAR(gamma) function in collagen-stimulated platelet activation. CONCLUSIONS: PPAR(gamma) agonists inhibit the activation of collagen-stimulation of platelet function through modulation of early GPVI signalling.


Assuntos
Plaquetas/efeitos dos fármacos , Fibrinolíticos/farmacologia , PPAR gama/agonistas , Agregação Plaquetária/efeitos dos fármacos , Glicoproteínas da Membrana de Plaquetas/metabolismo , Prostaglandina D2/análogos & derivados , Tiazolidinedionas/farmacologia , Trombose/prevenção & controle , Proteínas Adaptadoras de Transdução de Sinal/sangue , Anilidas/farmacologia , Animais , Plaquetas/metabolismo , Cálcio/sangue , Colágeno/sangue , Relação Dose-Resposta a Droga , Humanos , Immunoblotting , Peptídeos e Proteínas de Sinalização Intracelular/sangue , Ligantes , Proteínas de Membrana/sangue , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Selectina-P/sangue , PPAR gama/antagonistas & inibidores , PPAR gama/sangue , Fosforilação , Molécula-1 de Adesão Celular Endotelial a Plaquetas/sangue , Molécula-1 de Adesão Celular Endotelial a Plaquetas/genética , Prostaglandina D2/farmacologia , Proteínas Tirosina Quinases/sangue , Rosiglitazona , Transdução de Sinais/efeitos dos fármacos , Espectrometria de Fluorescência , Quinase Syk , Trombose/sangue , Fatores de Tempo , Tirosina/sangue
9.
J Appl Microbiol ; 105(6): 2153-60, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19016976

RESUMO

AIMS: To evaluate the ability of Streptomyces sp. (strain ASBV-1) to restrict aflatoxin accumulation in peanut grains. METHODS AND RESULTS: In the control of many phytopathogenic fungi the Streptomyces sp. ASBV-1 strain showed promise. An inhibitory test using this strain and A. parasiticus was conducted in peanut grains to evaluate the effects of this interaction on spore viability and aflatoxin accumulation. In some treatments the Streptomyces sp ASBV-1 strain reduced the viability of A. parasiticus spores by c. 85%, and inhibited aflatoxin accumulation in peanut grains. The values of these reductions ranged from 63 to 98% and from 67% to 96% for aflatoxins B(1) and G(1), respectively. CONCLUSIONS: It was demonstrated that Streptomyces sp. ASBV-1 is able to colonize peanut grains and thus inhibit the spore viability of A. parasiticus, as well as reducing aflatoxin production. SIGNIFICANCE AND IMPACT OF THE STUDY: The positive finding for aflatoxin accumulation reduction in peanut grains seems promising and suggests a wider use of this actinobacteria in biological control programmes.


Assuntos
Aflatoxinas/análise , Arachis/química , Aspergillus/fisiologia , Contaminação de Alimentos/prevenção & controle , Streptomyces/fisiologia , Arachis/microbiologia , Arachis/fisiologia , Cromatografia em Camada Fina , Grão Comestível , Espectrometria de Massas/métodos , Esporos Fúngicos/fisiologia
10.
Br J Pharmacol ; 154(5): 918-39, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18587441

RESUMO

Platelets have long been recognized to be of central importance in haemostasis, but their participation in pathological conditions such as thrombosis, atherosclerosis and inflammation is now also well established. The platelet has therefore become a key target in therapies to combat cardiovascular disease. Anti-platelet therapies are used widely, but current approaches lack efficacy in a proportion of patients, and are associated with side effects including problem bleeding. In the last decade, substantial progress has been made in understanding the regulation of platelet function, including the characterization of new ligands, platelet-specific receptors and cell signalling pathways. It is anticipated this progress will impact positively on the future innovations towards more effective and safer anti-platelet agents. In this review, the mechanisms of platelet regulation and current anti-platelet therapies are introduced, and strong, and some more speculative, potential candidate target molecules for future anti-platelet drug development are discussed.


Assuntos
Plaquetas/efeitos dos fármacos , Doenças Cardiovasculares/tratamento farmacológico , Drogas em Investigação/farmacologia , Hemostasia/efeitos dos fármacos , Inibidores da Agregação Plaquetária/farmacologia , Trombose/tratamento farmacológico , Animais , Plaquetas/metabolismo , Doenças Cardiovasculares/sangue , Desenho de Fármacos , Drogas em Investigação/uso terapêutico , Fibrinolíticos/farmacologia , Humanos , Inibidores da Agregação Plaquetária/uso terapêutico , Transdução de Sinais/efeitos dos fármacos , Trombose/sangue
11.
Braz J Med Biol Res ; 40(3): 383-9, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17334536

RESUMO

The effect of proton pump inhibitors and Helicobacter pylori infection on the bioavailability of antibiotics is poorly understood. We determined the effects of 5-day oral administration of 60 mg lansoprazole on the bioavailability of clarithromycin in individuals with and without H. pylori infection. Thirteen H. pylori-infected and 10 non-infected healthy volunteers were enrolled in a study with an open-randomized two-period crossover design and a 21-day washout period between phases. Plasma concentrations of clarithromycin in subjects with and without lansoprazole pre-treatment were measured by liquid chromatography coupled to a tandem mass spectrometer. Clarithromycin Cmax and AUC0-10 h were significantly reduced after lansoprazole administration. In addition, lansoprazole treatment of the H. pylori-positive group resulted in a statistically significant greater reduction in Cmax (40 vs 15%) and AUC0-10 h (30 vs 10%) compared to lansoprazole-treated H. pylori-negative subjects. Thus, treatment with lansoprazole for 5 days reduced bioavailability of clarithromycin, irrespective of H. pylori status. This reduction, however, was even more pronounced in H. pylori-infected individuals.


Assuntos
2-Piridinilmetilsulfinilbenzimidazóis/administração & dosagem , Antibacterianos/farmacocinética , Antiulcerosos/administração & dosagem , Claritromicina/farmacocinética , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori , Adulto , Antibacterianos/uso terapêutico , Área Sob a Curva , Disponibilidade Biológica , Estudos de Casos e Controles , Cromatografia Líquida de Alta Pressão , Claritromicina/uso terapêutico , Estudos Cross-Over , Sinergismo Farmacológico , Infecções por Helicobacter/metabolismo , Humanos , Lansoprazol , Inibidores da Bomba de Prótons , Fatores de Tempo
12.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;40(3): 383-389, Mar. 2007. tab
Artigo em Inglês | LILACS | ID: lil-441763

RESUMO

The effect of proton pump inhibitors and Helicobacter pylori infection on the bioavailability of antibiotics is poorly understood. We determined the effects of 5-day oral administration of 60 mg lansoprazole on the bioavailability of clarithromycin in individuals with and without H. pylori infection. Thirteen H. pylori-infected and 10 non-infected healthy volunteers were enrolled in a study with an open-randomized two-period crossover design and a 21-day washout period between phases. Plasma concentrations of clarithromycin in subjects with and without lansoprazole pre-treatment were measured by liquid chromatography coupled to a tandem mass spectrometer. Clarithromycin Cmax and AUC0-10 h were significantly reduced after lansoprazole administration. In addition, lansoprazole treatment of the H. pylori-positive group resulted in a statistically significant greater reduction in Cmax (40 vs 15 percent) and AUC0-10 h (30 vs 10 percent) compared to lansoprazole-treated H. pylori-negative subjects. Thus, treatment with lansoprazole for 5 days reduced bioavailability of clarithromycin, irrespective of H. pylori status. This reduction, however, was even more pronounced in H. pylori-infected individuals.


Assuntos
Humanos , Adulto , Antibacterianos/farmacocinética , Antiulcerosos/administração & dosagem , Claritromicina/farmacocinética , Helicobacter pylori , Infecções por Helicobacter/tratamento farmacológico , /administração & dosagem , Antibacterianos/uso terapêutico , Disponibilidade Biológica , Estudos de Casos e Controles , Cromatografia Líquida de Alta Pressão , Estudos Cross-Over , Claritromicina/uso terapêutico , Sinergismo Farmacológico , Bombas de Próton/antagonistas & inibidores , Fatores de Tempo
13.
J Mass Spectrom ; 40(9): 1197-202, 2005 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16127659

RESUMO

A simple, sensitive and specific liquid chromatography-tandem mass spectrometry method for the quantification of bromopride I in human plasma is presented. Sample preparation consisted of the addition of procainamide II as the internal standard, liquid-liquid extraction in alkaline conditions using hexane-ethyl acetate (1 : 1, v/v) as the extracting solvent, followed by centrifugation, evaporation of the solvent and sample reconstitution in acetonitrile. Both I and II (internal standard, IS) were analyzed using a C18 column and the mobile-phase acetonitrile-water (formic acid 0.1%). The eluted compounds were monitored using electrospray tandem mass spectrometry. The analyses were carried out by multiple reaction monitoring (MRM) using the parent-to-daughter combinations of m/z 344.20 > 271.00 and m/z 236.30 > 163.10. The areas of peaks from analyte and IS were used for quantification of I. The achieved limit of quantification was 1.0 ng/ml and the assay exhibited a linear dynamic range of 1-100.0 ng/ml and gave a correlation coefficient (r) of 0.995 or better. Validation results on linearity, specificity, accuracy, precision and stability, as well as application to the analysis of samples taken up to 24 h after oral administration of 10 mg of I in healthy volunteers demonstrated the applicability to bioequivalence studies.


Assuntos
Cromatografia Líquida de Alta Pressão , Antagonistas de Dopamina/sangue , Antagonistas de Dopamina/farmacocinética , Metoclopramida/análogos & derivados , Espectrometria de Massas por Ionização por Electrospray/métodos , Humanos , Metoclopramida/sangue , Metoclopramida/farmacocinética , Equivalência Terapêutica
14.
Anal Bioanal Chem ; 382(4): 1049-54, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15891869

RESUMO

A liquid chromatographic-tandem mass spectrometric method (LC-MS/MS) for quantifying amlodipine in human plasma was developed and validated. Sample preparation was based on liquid-liquid extraction using NaOH and a mixture of ethyl acetate/hexane (80/20; v/v). Chromatography was performed on a C-18 analytical column and the retention times were 1.9 and 3.0 min for amlodipine and nimodipine (internal standard), respectively. The ionization was optimized using ESI(+) and enhanced selectivity was achieved using tandem mass spectrometric analysis via two MRM functions, 409 --> 238 and 418 --> 343 for amlodipine and nimodipine. The calibration curve ranged from 0.2 to 20.0 ng/mL. The inter-day precision and accuracy and the relative standard deviation (RSD) were <15%. The analyte was shown to be stable over the time-scale of the whole procedure. The robustness of the method was demonstrated by the good reproducibility of the results obtained during the analysis of clinical samples.


Assuntos
Anlodipino/análise , Espectrometria de Massas em Tandem/métodos , Cromatografia Líquida/instrumentação , Cromatografia Líquida/métodos , Humanos , Estrutura Molecular , Sensibilidade e Especificidade , Espectrometria de Massas em Tandem/instrumentação
15.
J Mass Spectrom ; 37(2): 162-8, 2002 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11857760

RESUMO

Gas-phase reactions of four acylium ions and a thioacylium ion with three isomeric alpha-, beta- and gamma-hydroxy ketones are performed by pentaquadrupole mass spectrometric experiments. Novel structurally diagnostic reactions are observed, and found to correlate directly with interfunctional group separation. All five ions tested (CH(3)CO(+), CH(2)(double bond)CHCO(+), PhCO(+), (CH(3))(2)NCO(+) and (CH(3))(2)NCS(+)) react with the gamma-hydroxy ketone (5-hydroxy-2-pentanone) to form nearly exclusively a cyclic oxonium ion of m/z 85 that formally arises from hydroxy anion abstraction. With the beta-hydroxy ketone (4-hydroxy-2-pentanone), CH(2)(double bond)CHCO(+), PhCO(+) and (CH(3))(2)NCO(+) form adducts that undergo fast cyclization via intramolecular water displacement, yielding resonance-stabilized cyclic dioxinylium ions. With the alpha-hydroxy ketone (3-hydroxy-3-methyl-2-butanone), PhCO(+), (CH(3))(2)NCO(+) and (CH(3))(2)NCS(+) form stable adducts. Evidence that these adducts display cyclic structures is provided by the triple-stage mass spectra of the (CH(3))(2)NCS(+) adduct; it dissociates to (CH(3))(2)NCO(+) via a characteristic reaction-dissociation pathway that promotes sulfur-by-oxygen replacement. If cyclizations are assumed to occur with intramolecular anchimeric assistance, relationships between structure and reactivity are easily recognized.


Assuntos
Acetais/química , Cetonas/química , Acetais/análise , Espectrometria de Massas , Estrutura Molecular , Volatilização
16.
J Mass Spectrom ; 36(10): 1133-9, 2001 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11747107

RESUMO

A fast, sensitive and specific method is presented for the quantification of RSD921 in human plasma by liquid chromatography coupled with tandem mass spectrometry using tri-deuterated RSD921 (3d-RSD921) as an internal standard. A single-step liquid/liquid extraction was performed with diethyl ether/hexane (80 : 20, v/v) using 0.5 ml of plasma. The plasma calibration curves were linear from 0.1 to 20 ng ml(-1) (r > 0.999). Between-run precision, based on the percent relative deviation for replicate (n = 40) quality controls, was < or =7.27% (0.5 ng ml(-1)), < or =7.39% (5.0 ng ml(-1)), and < or =5.06% (20.0 ng ml(-1)). Between-run accuracies, based on the relative error, were +/-2.59%, +/-1.23% and +/-1.64% respectively. The method was developed to evaluate the pharmacokinetic profile after 15 min of intravenous stepwise-ascending infusion dose of RSD921 in 18 healthy volunteers. A dissociation study of protonated RSD921 and 3d-RSD921 by collision-induced dissociation using in-source fragmentation and tandem mass spectrometry is also presented.


Assuntos
Anestésicos Locais/sangue , Antiarrítmicos/sangue , Pirróis/sangue , Tiofenos/sangue , Anestésicos Locais/farmacocinética , Antiarrítmicos/farmacocinética , Calibragem , Cromatografia Líquida de Alta Pressão , Ensaios Clínicos Fase I como Assunto , Deutério , Humanos , Indicadores e Reagentes , Espectrometria de Massas , Pirróis/farmacocinética , Controle de Qualidade , Padrões de Referência , Tiofenos/farmacocinética
18.
J Org Chem ; 66(11): 3854-64, 2001 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-11375007

RESUMO

The intrinsic gas-phase reactivity of cyclic N-alkyl- and N-acyliminium ions toward addition of allyltrimethylsilane (ATMS) has been compared using MS(2) and MS(3) pentaquadrupole mass spectrometric experiments. An order of electrophilic reactivity has been derived and found to agree with orders of overall reactivity in solution. The prototype five-membered ring N-alkyliminium ion 1a and its N-CH(3) analogue 1b, as well as their six-membered ring analogues 1c and 1d, lack N-acyl activation and they are, accordingly, inert toward ATMS addition. The five- and six-membered ring N-acyliminium ions with N-COCH(3) exocycclic groups, 3a and 3b, respectively, are also not very reactive. The N-acyliminium ions 2a and 2c, with s-trans locked endocyclic N-carbonyl groups, are the most reactive followed closely by 3c and 3d with exocyclic (and unlocked) N-CO(2)CH(3) groups. The five-membered ring N-acyliminium ions are more reactive than their six-membered ring analogues, that is: 2a > 2c and 3c > 3d. In contrast with the high reactivity of 2a, its N-CH(3) analogue 2b is inert toward ATMS addition. For the first time, the transient intermediates of a Mannich-type condensation reaction were isolated-the beta-silyl cations formed by ATMS addition to N-acyliminium ions-and their intrinsic gas-phase behavior toward dissociation and reaction with a nucleophile investigated. When collisionally activated, the beta-silyl cations dissociate preferentially by Grob fragmentation, that is, by retro-addition. With pyridine, they react competitively and to variable extents by proton transfer and by trimethylsilylium ion abstraction-the final and key step postulated for alpha-amidoalkylation. Becke3LYP/6-311G(d,p) reaction energetics, charge densities on the electrophilic C-2 site, and AM1 LUMO energies have been used to rationalize the order of intrinsic gas-phase electrophilic reactivity of cyclic iminium and N-acyliminium ions.

19.
J Am Soc Mass Spectrom ; 12(2): 150-62, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11212000

RESUMO

A novel reaction of gaseous acylium ions: ketalization with diols and analogs, has been systematically studied via pentaquadrupole MS2 and MS3 experiments and ab initio calculations. A variety of alpha,beta-diols and their amino, thiol, ether, and thioether analogs have been tested for reactivity, mechanism evaluation, site selectivity, and for the effects of alpha- and beta-interfunctional separation. As for condensed-phase ketalization of neutral carbonyl compounds followed by hydrolysis, gaseous acylium ions are chemically deactivated in the form of cyclic ionic ketals by ketalization, and are efficiently released via on-line collision-induced dissociation. Ketalization of acylium ions is shown to identify and structurally characterize alpha,beta-diols and their analogs, and to distinguish regioisomers. Diastereomers can also be distinguished, as illustrated for cis and trans 1,2-diaminocyclohexane. The MS2 and MS3 data together with 18O-labeling and ab initio calculations establish for acylium ion ketalization a mechanism of anchimeric assistance with participation of the neighboring acyl group.

20.
J Am Soc Mass Spectrom ; 12(1): 14-22, 2001 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11142356

RESUMO

Primary carboxonium (H2C=O+-R) and carbosulfonium (H2C=S+-R) ions (R = CH3, C2H5, Ph) and the prototype five-membered cyclic carboxonium ion are found to react in the gas phase with cyclic acetals and ketals by transacetalization to form the respective O-alkyl-1,3-dioxolanium and S-alkyl-1,3-oxathiolanium ions. The reaction, which competes mainly with proton transfer and hydride abstraction, initiates by O-alkylation and proceeds by ring opening and recyclization via intramolecular displacement of the carbonyl compound previously protected in its ketal form. As indicated by product ion mass spectra, and confirmed by competitive reactions, carbosulfonium ions are, by transacetalization, much more reactive than carboxonium ions. For acyclic secondary and tertiary carboxonium ions bearing acidic alpha-hydrogens, little or no transacetalization occurs and proton transfer dominates. This structurally related reactivity distinguishes primary from both secondary and tertiary ions, as exemplified for the two structural isomers H2C=O+-C2H5 and CH3C(H)=O+-CH3. The prototype five- and six-membered cyclic carboxonium ions react mainly by proton transfer and adduct formation, but the five-membered ring ion also reacts by transacetalization to a medium extent. Upon CID, the transacetalization products of the primary ions often dissociate by loss of formaldehyde, and a +44 u neutral gain/-30 u neutral loss MS3 scan is shown to efficiently detect reactive carboxonium and carbosulfonium ions. Transacetalization with either carboxonium or carbosulfonium ions provides a route to 1,3-oxathiolanes and analogs alkylated selectively either at the sulfur or oxygen atom.

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