RESUMO
We investigated the potential role of mitochondrial manganese superoxide dismutase (Mn-SOD) in protective activity against irradiation by analyzing cell viability by a colony formation assay and by detecting apoptosis in stably human Mn-SOD gene-transfected HLE, a hepatocellular carcinoma cell line. We found that overexpression of Mn-SOD reduced the levels of reactive oxygen species in the mitochondria and intracellular phospholipid peroxidation product (4-hydroxy-2-nonenal) and prevented cell death. The production of intracellular nitric oxide after irradiation was not changed by Mn-SOD overexpression. The results suggested that Mn-SOD might play an important role in protecting cells against radiation-induced cell death by controlling the generation of mitochondrial reactive oxygen species and intracellular lipid peroxidation.
Assuntos
Carcinoma Hepatocelular/patologia , Morte Celular/efeitos da radiação , Mitocôndrias/enzimologia , Superóxido Dismutase/metabolismo , Apoptose/genética , Apoptose/efeitos da radiação , Carcinoma Hepatocelular/enzimologia , Morte Celular/genética , Divisão Celular/genética , Sobrevivência Celular/genética , Sobrevivência Celular/efeitos da radiação , Regulação Enzimológica da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Peroxidação de Lipídeos/efeitos da radiação , Mitocôndrias/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico/efeitos da radiação , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/genética , Fatores de Tempo , Transfecção , Células Tumorais CultivadasRESUMO
Congenital absence of the terminal portion of the portal vein with visceral venous return to the suprahepatic inferior vena cava, a rare malformation, was demonstrated in an 18-year-old Japanese woman. She had nodular hyperplasia in the liver and a non-functioning pancreatic endocrine tumour. It is generally believed that reduction of portal venous flow causes atrophic changes and, subsequently, nodular hyperplasia occurs in a well-perfused area in the liver. However, the liver was not perfused by the portal vein in this case. It is suggested that nodular hyperplasia can occur without portal blood flow.