CNT-FET for sensitive hydrogen peroxide biosensing via immobilized Cytochrome c.

H2O2 is an effective substance in the body which contributes to gene expression, insulin metabolism and determining cell shapes. However, a high concentration of H2O2 is harmful to the body and can cause various diseases such as colitis wounds, sepsis disease, lymphocyte proliferation and macrophage apoptosis in systemic lupus erythematosus. In this study, a Cyt c/cMWCNTs/FET was designed to real-time detect H2O2 via immobilized Cyt c on the cMWCNTs/FET surface. The performance of the Cyt c/cMWCNTs/FET biosensor was studied under various parameters such as cMWCNTs and Cyt c concentrations, as well as different pH values. When H2O2 was added to the reaction chamber of the Cyt c/cMWCNTs/FET, the output current of the Bio-FET was reduced, which was attributed to H2O2 detection. The linear response range of this Cyt c/cMWCNT/FET was 10.0 fM to 1.0 nM. The limit of detection and response time of this platform were determined to be 9.13 fM and around 1.0 s, respectively. Also, the operation of the Cyt c/cMWCNTs/FET in the presence of glucose, leucine, tyrosine and ascorbic acid as interfering substances was selective towards H2O2.

Long-Term Vaccine Delivery and Immunological Responses Using Biodegradable Polymer-Based Carriers.

Biodegradable polymers are largely employed in the biomedical field, ranging from tissue regeneration to drug/vaccine delivery. The biodegradable polymers are highly biocompatible and possess negligible toxicity. In addition, biomaterial-based vaccines possess adjuvant properties, thereby enhancing immune responses. This Review introduces the use of different biodegradable polymers and their degradation mechanism. Different kinds of vaccines, as well as the interaction between the carriers with the immune system, then are highlighted. Natural and synthetic biodegradable micro-/nanoplatforms, hydrogels, and scaffolds for local or targeted and controlled vaccine release are subsequently discussed.