Antimicrobial defenses of table eggs: Importance of antibacterial proteins in egg white as a function of hen age in an extended production cycle.

The importance of egg natural defences to prevent bacterial contamination and their relation with hen age in extended production cycles were evaluated. Egg-white from eggs of different hen age groups (up 100-weeks-old) and lines (Hy-Line white and brown) were inoculated with Gram-positive Staphylococcus aureus or Gram-negative Salmonella Typhimurium, ranging from 103-106 CFU/mL. Our results show that concentrations of egg-white lysozyme and, particularly, ovotransferrin are important to modulate bacterial survival in a dose-dependent matter. Depending on protein concentration, their effect ranges from bactericidal to bacteriostatic, with a threshold for bacterial contamination that depends also on hen age and line. The concentrations of lysozyme and ovotransferrin increased with hen age (up to 2 and 22 w/w% of total protein, respectively), and eggs laid by older hens exhibited the greatest potential to prevent the growth of the highest Salmonella inoculum (106 CFU/mL). Salmonella-penetration experiments demonstrated that non-contaminated eggs display significantly higher concentrations of antimicrobial proteins. However, eggs from older hens needed a higher concentration of these proteins (>20% ovotransferrin) to prevent bacterial contamination, showing that antimicrobial protein concentrations in egg-whites was not the only factor influencing bacterial contamination. Finally, this study demonstrated that egg-white of eggs produced by old hens are less prone to contamination by Salmonella.

What gypsy mothers say about breastfeeding.

OBJECTIVE: To know and deepen in the evaluation, and experience of gypsy women with breastfeeding. METHOD: Qualitative study with an interpretative phenomenological approach, through a focus group with the participation of six mothers of gypsy ethnicity, users of the Fuente de San Luis Health Center in the city of Valencia. RESULTS: The barriers described by these mothers regarding breastfeeding refer to a lack of family support, an association of breastfeeding to sacrifice and to the dependence of the baby to the breast. From the accounts of these mothers it is perceived, a disinterest in breastfeeding and a lack of confidence in their own bilogy, which together with a low participation in maternal education activities, have meant the barriers that have mostly limited and largely hindered breastfeeding in this group of mothers. CONCLUSIONS: It would be desirable to implement improvement actions that would pay more attention to promote, protect and support breastfeeding in this group.

[A proposal of sociological classification of Internet use in primary health care]. / Estrategias ante el uso de Internet por población usuaria en Atención Primaria: una clasificación sociológica.

OBJECTIVE: To analyze and classify the communication strategies developed by primary health care professionals in the Valencian Community (Spain), in view of the use of the Internet as a source of health information by the user population through the analytical categories of power and control which, according to Basil Bernstein, characterize the communication processes of therapeutic meetings. METHOD: Qualitative study through in-depth interviews with primary health care professionals of the Valencian Community (n=18). The language of description of Bernstein's theory was applied to the analysis of the speeches in order to classify the strategies developed by the professionals and to propose a typology. RESULTS: A sociological typology of strategies used by professionals in the face of Internet use by the user population is provided, which classifies them according to the classification and framing modulation in rejection strategies, validation strategies, filtering strategies and opening strategies. CONCLUSIONS: The different strategies developed by professionals when faced with the use of the Internet as a source of information have different implications for the power and control relationships inherent to the therapeutic encounter. Professionals should be aware of these implications in order to increase the participation of the user population in therapeutic meetings.

Cruising y e-citas: un nuevo contexto para los encuentros sexuales entre hombres jóvenes que tienen sexo con hombres / Cruising e e-compromissos: um novo contexto de encontros sexuais entre os jovens homens que fazem sexo com homens / Cruising and e-dates: a new context for sexual encounters among young men who have sex with men

Resumen El objetivo de este artículo es el de explorar el papel que juega, en la actualidad, la irrupción de la mediación tecnológica de la información y la comunicación en la configuración de nuevos contextos para la relación sexual. En concreto, hemos explorado una práctica determinada, el cruising, entre hombres jóvenes que tienen sexo con hombres contactado a través de las e-citas. Se ha apostado por la metodología cualitativa con la intención de recopilar los discursos sobre sexualidad, riesgos y salud que se generan y construyen entre el grupo de iguales. Según extraemos de nuestro estudio, el nuevo marco que confiere esta tecnología estaría caracterizado por (a) una mayor accesibilidad a dichas prácticas, (b) una mayor difusión de estas, (c) una mayor inmediatez de acceso a las propuestas, (d) la anticipación de lo que será ofrecido en la cita, (e) acceso anónimo que permite propuestas más atrevidas y (f) una apertura mayor a la diversidad de usuarios. Todos estos elementos confluyen en un cuadro que nos lleva a considerar una organización social renovada de la práctica sexual.

Resumo O objetivo deste artigo é explorar o papel que a irrupção da mediação tecnológica da informação e da comunicação desempenha na definição de novos contextos para a relação sexual. Em particular, explora-se uma determinada prática, o cruising entre os homens jovens que fazem sexo com homens contatados via e- encontros. Nossa aposta foi pela metodologia qualitativa, com a intenção de recolher os discursos sobre a sexualidade, a saúde e os riscos que são gerados e construídos entre o grupo de pares. De acordo com o nosso estudo, o novo quadro que oferece essa tecnologia seria marcado por: (a) uma maior acessibilidade a essas práticas; (b) uma maior divulgação das mesmas, bem como (c) um maior imediatismo de acesso para as propostas; (d) a antecipação do que vai ser oferecido no encontro; (e) acesso anônimo que permite propostas mais ousadas; e (f) uma maior abertura para a diversidade de usuários. Todos esses elementos convergem em um contexto que leva-nos a considerar uma nova organização social da prática sexual.

Abstract This article addresses the rapidly growing use of information and communication technologies in mediating new settings for sexual relations. The specific focus is on cruising among young men who have sex with men via e-dating. The study used a qualitative methodology with peer group discussions on sexuality, health, and risk. The new framework provided by technology is marked by (a) increased access to these practices, (b) greater dissemination of them, (c) greater immediacy of encounters, (d) anticipation of what will be offered during dates, (e) anonymous access, allowing bolder proposals, and (f) openness to a wider range of users. These elements converge in a framework that indicates a new social organization of sexual practice.

Cruising and e-dates: a new context for sexual encounters among young men who have sex with men.

This article addresses the rapidly growing use of information and communication technologies in mediating new settings for sexual relations. The specific focus is on cruising among young men who have sex with men via e-dating. The study used a qualitative methodology with peer group discussions on sexuality, health, and risk. The new framework provided by technology is marked by (a) increased access to these practices, (b) greater dissemination of them, (c) greater immediacy of encounters, (d) anticipation of what will be offered during dates, (e) anonymous access, allowing bolder proposals, and (f) openness to a wider range of users. These elements converge in a framework that indicates a new social organization of sexual practice.

Change in the chicken eggshell cuticle with hen age and egg freshness.

For a fuller understanding of the functionality of the eggshell cuticle, we conducted a detailed study using a wide array of analytical techniques (scanning and transmission microscopy), energy dispersive x-rays, and attenuated total reflection-Fourier transform infrared spectroscopy to analyze the structure, morphology, and chemical composition of this organic coating. This study shows that the cuticle has a compositional gradation with an outer part richer in proteins and an inner part richer in sulfated polysaccharides and phosphates. It also shown that the cuticle composition, thickness, and degree of coverage are highly dependent on hen age and egg freshness. During the course of the first laying year, the thickness and degree of glycosylation of the cuticle decreases with hen age, and at the end of the laying cycle, the cuticle is significantly depleted in lipids. There are also well-defined compositional changes in the cuticle of freshly laid eggs as time passes and there is a notable increase in the permeability of the eggshell after 24 h due to cuticle drying. We discuss how these changes in the cuticle can affect the food safety of eggs in relation to the risk of trans-shell contamination by bacteria (i.e., Salmonellosis).

Perceptions and experiences of parenthood and maternal health care among Latin American women living in Spain: a qualitative study.

OBJECTIVE: to explore the experiences and perceptions of parenthood and maternal health care among Latin American women living in Spain. DESIGN: an exploratory qualitative research using focus groups and thematic analysis of the discussion. SETTING AND PARTICIPANTS: three focus groups with 26 women from Bolivia and Ecuador and three focus groups with 24 midwives were performed in three towns in the Valencian Community receiving a large influx of immigrants. FINDINGS: the women interpreted motherhood as the role through which they achieve fulfilment and assumed that they were the ones who could best take care of their children. They perceived that men usually make decisions about sex and pregnancy and recognised a poor or inadequate use of contraceptive methods in planning their pregnancies. Women reported that it was not necessary to go as soon and as frequently for health examinations during pregnancy as the midwives suggested. The main barriers identified to health-care services were linked to insecure or illegal employment status, inflexible appointment timetables for prenatal checkups and sometimes to ignorance about how public services worked. KEY CONCLUSIONS AND IMPLICATIONS FOR PRACTICE: empowering immigrant women is essential to having a long-term positive effect on their reproductive health. Antenatal care providers should be trained to build maternity care that is culturally sensitive and responds better to the health needs of different pregnant women and their newborns.

Biocontrol of psychrotrophic enterotoxigenic Bacillus cereus in a nonfat hard cheese by an enterococcal strain-producing enterocin AS-48.

Bacillus cereus is a food poisoning bacterium of great concern, especially in milk products. In this study, we describe the efficient control of the psychrotrophic and toxigenic strain B. cereus LWL1 in milk and in a nonfat hard cow's cheese by the enterocin AS-48 producer strain Enterococcus faecalis A-48-32 (Bac+). No viable B. cereus cells were detected after 72 h incubation in milk coinoculated with the AS-48-producing strain and B. cereus. Diarrheic toxin production was also markedly inhibited by the Bac+ strain to eightfold lower levels compared with control cultures of B. cereus. In cheeses manufactured by inoculation with a commercial starter (about 6.8 log CFU/ml) and B. cereus (about 4 log CFU/ml), the latter reached 6.27 log CFU/g after 5 days of maturation, and approximately 8 log CFU/g after 15 days. However, in cheeses made from milk inoculated with the starter along with a mixture of E. faecalis-B. cereus (2/1 ratio), counts of B. cereus decreased by approximately 1.0, 2.0, 4.32, and 5.6 log units with respect to control cheeses after 5, 10, 15, and 30 days of ripening, respectively. Growth of E. faecalis A-48-32 was associated with enterocin AS-48 production and persistence in cheese. Interestingly, growth of starter cultures was not affected by the Bac+ strain, and neither was lactic acid production. These results clearly indicate that E. faecalis A-48-32 produced satisfactory amounts of bacteriocin in cheese and support the potential use of AS-48-producing strains as culture adjuncts to inhibit B. cereus during cheese manufacture and ripening.