Evaluation of antibiofilm activity of metal oxides nanoparticles and carbon nanotubes coated styrofoam on the bacterium Jeotgalicoccus huakuii.

The co-existence of metal oxide nanoparticles (MONPs), carbon-based nanomaterials and microplastics (MPs) in the natural environment are expected to be of growing global concern due to their increasing abundance and persistence in the environment worldwide. Knowledge of the interaction of the above compounds particularly under light irradiation in water remains limited. In the present study, the possible individual and combined toxic effects of MONPs, carbon nanotubes (CNTs) through styrofoam (SF) on the environmental bacterium Jeotaglicoccus huakuii were systematically investigated. The fabricated MONPs and CNTs were characterized using the following techniques: FT-IR (functional groups), XRD (crystallinity), SEM, and EDX (topological morphology). The objective of this study was to investigate and identify naturally occurring bacteria capable of mitigating and detoxifying toxic pollutants under adverse conditions. Moreover, the assessment of minimum inhibitory concentration (MIC) was made through an agar well plate method, resazurin (ELISA measurement), growth kinetics and bacterial viability were assessed employing live and dead assay and biofilm combating ability was analyzed using an antibiofilm assay. Further, the biotransformation of f-MWCNTs by J. huakuii was evaluated employing RT-PCR and SEM analysis. The results demonstrated that the toxicity of Pb3O4@f-MWCNTs was comparatively higher than the remaining Pb3O4 NPs and SF coated NPs.. Interestingly, J. huakuii showed resistance against f-MWCNTs at very high concentrations and able to utilize f-MWCNTs as a sole carbon source suggesting J. huakuii as a suitable aquatic bioremediation tool for both MONPs and CNTs transfer via MPs. The results also enhanced our understanding of the affinity of MPs towards MONPs and CNTs under extreme environmental conditions.

Adipose-derived stem cells loaded photocurable and bioprintable bioinks composed of GelMA, HAMA and PEGDA crosslinker to differentiate into smooth muscle phenotype.

Developing a polymer-based photocrosslinked 3D printable scaffolds comprised of gelatin methacryloyl (G) and hyaluronic acid methacryloyl (H) incorporated with two molecular weights of polyethylene glycol diacrylate (P) of various concentrations that enables rabbit adipose-derived stem cells (rADSCs) to survive, grow, and differentiate into smooth muscle cells (SMCs). Then, the chemical modification and physicochemical properties of the PGH bioinks were evaluated. The cell viability was assessed via MTT, CCK-8 assay and visualized employing Live/Dead assay. In addition, the morphology and nucleus count of differentiated SMCs were investigated by adopting TRAP (tartrate-resistant acid phosphatase) staining, and quantitative RT-PCR analysis was applied to detect gene expression using two different SMC-specific gene markers α-SMA and SM-MHC. The SMC-specific protein markers namely α-SMA and SM-MHC were applied to investigate SMC differentiation ability by implementing Immunocytofluorescence staining (ICC) and western blotting. Moreover, the disk, square, and tubular cellular models of PGH7 (GelMA/HAMA=2/1) + PEGDA-8000 Da, 3% w/v) hybrid bioink were printed using an extrusion bioprinting and cell viability of rADSCs was also analysed within 3D printed square construct practising Live/Dead assay. The results elicited the overall viability of SMCs, conserving its phenotype in biocompatible PGH7 hybrid bioink revealing its great potential to regenerate SMCs associated organs repair.

Assessing the Effects of Ozonation on the Concentrations of Personal Care Products and Acute Toxicity in Sludges of Wastewater Treatment Plants.

The aim of this study was to understand the distribution of the personal care products nonylphenol (NP), triclosan (TCS), benzophenone-3 (BP-3), and caffeine in the sludges from three wastewater treatment plants (WWTP-A, -B, and -C) in southern Taiwan. The four compounds were analyzed from activated sludge and dewatered sludge samples, and then the samples were treated with pressure-assisted ozonation under different conditions and removal efficiencies. All four target compounds were detected, especially NP, which was detected in the highest concentrations in the activated sludges of WWTP-A and dewatered sludges of WWTP-C at 17.19 ± 4.10 and 2.41 ± 1.93 µg/g, respectively. TCS was dominant in dewatered sludges from WWTP-B, and the highest detected concentration was 13.29 ± 6.36 µg/g. Removals of 70% and 90% were attained under 150 psi at 40 cycles for NP and TCS, respectively, with 5 min of ozonation reaction time, a solid/water ratio of 1:20, and 2% ozone concentration. Ecological risk quotients (RQs) were calculated by the ratios of the 10-day Hyalella azteca (freshwater amphipod) LC50 to the environmental concentrations of the target compounds. High RQs were found to be >10 for NP, TCS, and BP-3 in untreated sludges, resulting in significant ecological risks to aquatic organisms when the sludges are arbitrarily disposed. However, the toxic effects on Hyalella azteca were not significantly different among ozone sludge treatments. The reason for this may be related to the formation of toxic oxidation by-products and incomplete mineralization of organic compounds. This could also be true for unknown intermediates. The relatively high detection frequencies of these emerging compounds in WWTP sludges requires further applications and treatments.

Combined effects of copper oxide and nickel oxide coated chitosan nanoparticles adsorbed to styrofoam resin beads on hydrothermal vent bacteria.

Microplastics are potential carriers of harmful contaminants but their combined effects are largely unknown. It needs intensive monitoring in order to achieve a better understanding of metal-oxide nanoparticles and their dispersion via microplastics such as styrofoam in the aquatic environment. In the present study, an effort was made to provide a preferable perception about the toxic effects of engineered nanoparticles (NPs), namely, copper oxide (CuO NPs), nickel oxide (NiO NPs), copper oxide/chitosan (CuO/CS NPs) and nickel oxide/chitosan (NiO/CS NPs). Characterizations of synthesized NPs included their morphology (SEM and EDX), functional groups (FT-IR) and crystallinity (XRD). Their combined toxic effect after adsorption to styrofoam (SF) was monitored using the hydrothermal vent bacterium Jeotgalicoccus huakuii as a model. This was done by determining MIC (minimum inhibitory concentration) through a resazurin assay measuring ELISA, growth, biofilm inhibition and making a live and dead assay. Results revealed that at high concentrations (60 mg/10 mL) of CuO, CuO/CS NPs and 60 mg of SF adsorbed CuO and CuO/CS NPs inhibited the growth of J. huakuii. However, NPs rather than SF inhibited the growth of bacteria. The toxicity of NPs adsorbed on plain SF was found to be less compared to NPs alone. This study revealed new dimensions regarding the positive impacts of SF at low concentrations. Synthesized NPs applied separately were found to affect the growth of bacteria substantially more than if coated to SF resin beads.

Efficacy and side effects of bio-fabricated sardine fish scale silver nanoparticles against malarial vector Anopheles stephensi.

Mosquitoes are a great menace for humankind since they transmit pathogenic organisms causing Malaria, Dengue, Chikungunya, Elephantiasis and Japanese encephalitis. There is an urgent need to discover new and novel biological tools to mitigate mosquito-borne diseases. To develop bioinsecticides through newly developed nanotechnology is another option in the present research scenario. In this study we synthesize and characterize sardine fish scales with silver nitrate by adopting various instrumental techniques such as UV- and FTIR-spectroscopy, energy-dispersive X-ray (EDAX), X-ray diffraction analyses (XRD) and scanning electron microscopy (SEM). Toxicity bioassays were conducted with young developmental stages of mosquito vectors. Significant mortality appeared after different life stages of mosquito vectors (young larval and pupal instars were exposed to the nanomaterials). LC50 values were 13.261 ppm for young first instar larvae and 32.182 ppm for pupae. Feeding and predatory potential of G. affinis, before and after exposure to nanoparticles against mosquito larval (I & II) instars of the mosquitoes showed promising results in laboratory experiments. Feeding potential of mosquito fish without nanoparticle treatment was 79.7% and 70.55% for the first and second instar larval populations respectively. At the nanoparticle-exposed situation the predatory efficiency of mosquitofish was 94.15% and 84.3%, respectively. Antioxidant enzymes like (SOD), (CAT), and (LPO) were estimated in the gill region of sardine fish in control and experimental waters. A significant reduction of egg hatchability was evident after nanoparticle application. It became evident from this study that the nano-fabricated materials provide suitable tools to control the malaria vector Anopheles stephensi in the aquatic phase of its life cycle. This finding suggests an effective novel approach to mosquito control.