RESUMO
In vitro micropropagation study of Aloe monticola Reynolds was conducted using offshoots in Murashige and Skoog (MS) media enriched with plant growth regulators (PGRs). Initiation experiment, carried out by culturing sterilized explants in full-strength MS media enriched with 0.0-1.0 mg/L benzyl amino purine (BAP) alone and in combination with 0.10 mg/L indole-3-butyric acid (IBA), resulted in 89 - 100 % healthy and live (i.e., initiated) explants in 9-29 days. Regeneration study, conducted by culturing initiated explants in full-strength MS media supplemented with 0.0-3.0 mg/L BAP alone and in combination with 0.50 mg/L IBA, showed that treatments enriched with 1.0-3.0 mg/L BAP in combination with 0.50 mg/L IBA yielded better shooting responses than the rest of the treatments. The rooting responses of the shoots were also tested by culturing in half-strength MS media enriched with 0.0-4.0 mg/L NAA alone and in combination with 0.25 mg/L IBA. Better rooting responses were observed in treatments supplemented with 1.0-4.0 mg/L NAA in combination with 0.25 mg/L IBA with two exceptions. The responses of A. monticola plantlets to primary and secondary acclimatization in greenhouse, nursery shade, and direct sunlight in coco peat, composted soil, and manured soil were excellent - with survival percentages of 90-98 %. The findings of this empirical research are important for developing refined protocol of in vitro micropropagation of this ecologically important but endangered plant.
RESUMO
The purpose of the research is to study the development of response surface methodology for optimization of chebulinic acid extraction from composition of medicinal herbs such as Terminalia chebula, Phyllanthus emblica and seeds of Dimocarpus longan. Optimization of extraction parameters such as weight dosages, pH and time were carried out by response surface methodology (RSM). The optimal conditions determined for extraction of chebulinic acid through response surface methodology were dosage (6.25â¯g), pH (5.7) and time (24.23 h). These results showed that the developed model is satisfactory and relevant for the extraction of chebulinic acid. The analysis of variance showed a high goodness of model fit and the performance of the RSM method for improving chebulinic acid extraction from the composition of medicinal herbs. Quantitative estimation of chebulinic acid in the composition of medicinal herbs by HPLC studies revealed that 0.712% w/w of chebulinic acid content was present in the composition of herbal powder.