RESUMO
East Coast fever, caused by the protozoan parasite Theileria parva, kills about 600,000 cattle annually in Africa. The hydroxynaphthoquinone compound buparvaquone (BPQ) is curative. Sixteen calves were infected with T. parva. On manifestation of disease symptoms, eight were injected with the original (pioneer) BPQ product and eight with a test product containing BPQ. All 16 calves were cured by one injection of 2.5 mg BPQ/kg bodyweight. The concentration of BPQ in blood plasma was monitored by HPLC. The mean observed C(max) of BPQ was 0.229 and 0.253 microg/mL of plasma, the mean observed time to reach this concentration (T(max)) was 2.62 and 2.12 h and the AUC (area under curve) was 4.785 and 4.156 microg h/mL, respectively, for the pioneer and test product. Considerable variations occurred in the plasma concentration of BPQ within each group. They showed no relationship with either clinical or parasitological parameters following treatment.
Assuntos
Antiprotozoários/farmacologia , Antiprotozoários/farmacocinética , Doenças dos Bovinos/tratamento farmacológico , Naftoquinonas/farmacologia , Naftoquinonas/farmacocinética , Theileriose/tratamento farmacológico , Animais , Antiprotozoários/administração & dosagem , Área Sob a Curva , Bovinos , Doenças dos Bovinos/parasitologia , Masculino , Naftoquinonas/administração & dosagemRESUMO
An observational longitudinal study was carried out on 92 randomly selected smallholder farms in two coastal lowland zones of Kwale District in Kenya between December 1997 and November 1999. The objective was to estimate the incidence of the main vector-transmitted diseases in pre-weaned calves. From an initial 41 pure or cross-bred Bos taurus calves which were less than 2 months and whose birth and disease histories were known, study calves were recruited progressively and monitored until they were weaned at around 146 days. Overall, 130 calves in 67 farms were monitored and these contributed a total risk period of 30,062 days. Disease parameters were analysed and compared as true annual and age-specific incidence rates. The incidences of East Coast fever (ECF) (23.1%) and trypanosomosis (29.1%) were the highest among the vector-borne diseases. The corresponding mortality incidence rates of ECF and trypanosomosis were 10.9 and 3.6%, respectively. The annual incidence rates of anaplasmosis and babesiosis were 10.9 and 1.2%, respectively. There was no mortality arising specifically from anaplasmosis or babesiosis. Analysis of survival times to natural infection indicated that the field challenge resulting to cases of trypanosomosis was much higher compared to the risk of either ECF or anaplasmosis. It was concluded that these vector-borne diseases constrain production of replacement stock in this coastal lowlands region of Kenya.
Assuntos
Anaplasmose/epidemiologia , Doenças dos Bovinos/epidemiologia , Doenças Transmitidas por Carrapatos/veterinária , Tripanossomíase Africana/veterinária , Tripanossomíase Bovina/epidemiologia , Envelhecimento , Animais , Bovinos , Doenças dos Bovinos/mortalidade , Indústria de Laticínios , Feminino , Incidência , Quênia/epidemiologia , Masculino , Doenças Transmitidas por Carrapatos/epidemiologia , Tripanossomíase Africana/epidemiologiaRESUMO
A study was carried out on 92 smallholder farms in Kwale district in Coast Province of Kenya to estimate the milk yield. The effect of concentrate feed supplementation on milk yield was also evaluated. Data were collected during a one-year observational longitudinal study. Analysis was done for 371 observations following 63 calving events. The mean annual milk offtake was estimated at 2021 kg/cow. Forty-nine (77.8%) of the lactating cows were supplemented with concentrate feeds at varying rates of 0.5-3.0 kg/cow per day. Supplementary feeding of lactating cows led to a significantly higher mean daily milk yield compared to non-supplemented cows throughout the year (p < 0.05). The mean annual milk offtake from supplemented cows (2195 kg/cow) was 18.6% more than offtake from non-supplemented cows, a difference that was statistically significant (p < 0.05). Therefore, supplementary feeding of commercial feed concentrates was a rational management practice. It was also concluded that milk production from smallholder dairy cows in the coastal lowlands of Kenya was comparable to that from similar production systems but lower than national targets.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal , Bovinos/fisiologia , Indústria de Laticínios , Leite/metabolismo , Ração Animal , Animais , Indústria de Laticínios/métodos , Indústria de Laticínios/normas , Suplementos Nutricionais , Ingestão de Alimentos , Feminino , Quênia , Lactação/metabolismo , Lactação/fisiologia , Estudos Longitudinais , Estações do AnoRESUMO
A total of 90 animals was immunized against East Coast fever (ECF) using Theileria parva (Marikebuni) stock on three large-scale farms in Kiminini Division, Trans-Nzoia District, North Rift, Kenya. Another 90 cattle served as non-immunized controls. Following immunization the number of cattle with significant indirect fluorescent antibody (IFA) titres increased from 43.9% to 84.4% and 6.7% of the cattle developed clinical ECF reactions. Two months after immunization, the immunized and non-immunized cattle were divided into two groups one of which was dipped every 3 weeks and the other dipped when total full body tick counts reached 100. All the animals were monitored for 51 weeks for incidences of ECF and other tick-borne diseases. Twenty-four cases of ECF were diagnosed among the non-immunized cattle compared to four cases among the immunized cattle; a difference that was significant (P > 0.05). There was no significant difference in the incidences of babesiosis and anaplasmosis between the immunized and non-immunized cattle.
Assuntos
Imunização/veterinária , Theileria parva/imunologia , Theileriose/prevenção & controle , Anaplasmose/epidemiologia , Anaplasmose/prevenção & controle , Animais , Anticorpos Antiprotozoários/sangue , Babesiose/epidemiologia , Babesiose/prevenção & controle , Babesiose/veterinária , Bovinos , Técnica Indireta de Fluorescência para Anticorpo/métodos , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Quênia/epidemiologia , Distribuição Aleatória , Theileriose/epidemiologia , Theileriose/imunologia , Controle de Ácaros e Carrapatos/métodos , Infestações por Carrapato/epidemiologia , Infestações por Carrapato/prevenção & controle , Infestações por Carrapato/veterinária , Fatores de TempoRESUMO
Appraisal of the indirect fluorescent antibody test (IFAT) and antigen enzyme linked immunosorbent assay (ELISA) serological tests as carried out to detect cattle exposed to Theileria parva at the National Veterinary Research Centre, Muguga (NVRC), Kenya is reported. Using sera from T. parva naive cattle and cattle experimentally exposed to T. parva, the two tests were appraised in terms of their sensitivity and specificity. IFAT and ELISA had the same sensitivity of 90% while ELISA had a higher specificity (90%) than IFAT (80%). A comparison was also made of the capability of the two tests to detect exposure of dairy cattle to T. parva prior to immunization against East Coast fever (ECF). The positive outcome from the IFAT was significantly higher (chi 2 = 30.36; P < 0.001) than that from the ELISA. The agreement between the two tests was low (Kappa = 0.21). The two tests indicated a higher risk of ECF in the study area than was expected. Indications are that the ELISA has been effectively adopted at NVRC.
Assuntos
Anticorpos Antiprotozoários/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Imunização , Theileria parva/imunologia , Theileriose/imunologia , Animais , Bovinos , Interpretação Estatística de Dados , Técnica Indireta de Fluorescência para Anticorpo , Quênia/epidemiologia , Masculino , Valor Preditivo dos Testes , Prevalência , Sensibilidade e Especificidade , Theileriose/diagnóstico , Theileriose/epidemiologiaRESUMO
Two trials were conducted to compare the efficacy of parvaquone and buparvaquone for the treatment of naturally acquired East Coast fever (ECF, Theileria parva infection) which, if untreated, is almost invariably fatal in European breeds of cattle. In the first trial 28 naive cattle were exposed in a paddock infested with ticks carrying a virulent form of the disease. Twelve were treated with each drug when they developed clinical ECF. All 24 cattle were cured. In the second study, 100 cases of ECF occurring naturally on farms in Kenya were treated, 50 with each drug. Parvaquone cured 44 (88%) buparvaquone cured 45 (90%). Intercurrent infections, predominantly anaplasmosis and bacterial pneumonia or scour, were treated specifically. It is concluded that parvaquone and buparvaquone are similarly effective in curing ECF and cure rates are maximised by accurate diagnosis and prompt treatment of both ECF and intercurrent infections.
Assuntos
Antimaláricos/uso terapêutico , Naftoquinonas/uso terapêutico , Theileriose/tratamento farmacológico , Administração Tópica , Animais , Antimaláricos/administração & dosagem , Biópsia/veterinária , Bovinos , Feminino , Febre/veterinária , Injeções Intramusculares/veterinária , Inseticidas/uso terapêutico , Quênia , Masculino , Naftoquinonas/administração & dosagem , Nitrilas , Parasitemia/veterinária , Piretrinas/uso terapêutico , Distribuição Aleatória , Theileriose/sangue , Infestações por Carrapato/veterináriaRESUMO
A spreadsheet model was developed and used to estimate the total cost of immunising cattle against East Coast fever (ECF) based on the infection-and-treatment method. Using data from an immunisation trial carried out on 102 calves and yearlings on 64 farms in the Githunguri division, Kiambu district, Kenya, a reference base scenario of a mean herd of five animals, a 10% rate of reaction to immunisation and a 2-day interval monitoring regimen (a total of 10 farm visits) was simulated. Under these conditions, the mean cost of immunisation per animal was US$16.48 (Ksh 955.78); this was equivalent to US$82.39 (Ksh 4778.90) per five-animal farm. A commonly reported reactor rate of 3% would decrease the cost of US$14.63 (Ksh 848.29) per animal. Reducing the number of farm monitoring visits from 10 to 7 would reduce the total cost by 10%, justified if farmers are trained to undertake some of the monitoring work. The fixed costs were 53% of the total cost of immunisation per farm. The cost of immunisation decreased with increasing number of animals per farm, showing economies of scale.