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Cholesteric liquid crystals exhibit a periodic helical structure that partially reflects light with wavelengths comparable to the period of the structure, thus performing as a one-dimensional photonic crystal. Here, we demonstrate a combined experimental and numerical study of light transmittance spectra of finite-length helical structure of cholesteric liquid crystals, as affected by the main system and material parameters, as well as the corresponding eigenmodes and frequency eigenspectra with their Q-factors. Specifically, we have measured and simulated transmittance spectra of samples with different thicknesses, birefringences and for various incident light polarisation configurations as well as quantified the role of refractive index dispersion and the divergence of the incident light beam on transmittance spectra. We identify the relation between transmittance spectra and the eigenfrequencies of the photonic eigenmodes. Furthermore, we present and visualize the geometry of these eigenmodes and corresponding Q-factors. More generally, this work systematically studies the properties of light propagation in a one-dimensional helical cholesteric liquid crystal birefringent profile, which is known to be of interest for the design of micro-lasers and other soft matter photonic devices.
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Recently, the design and fabrication of light-powered actuators have attracted immense attention because of the manufacturing of intelligent soft robots and innovative self-regulating devices. Accordingly, a liquid crystal polymer network (LCN) provides a promising platform due to its reversible and multistimulus-responsive shape-changing behaviors. In particular, doping nanoparticles with exclusive properties into the LCN can produce interesting results. In this work, we investigated a TiO2 nanoparticle-based LCN polymer light-powered actuator. TiO2 nanoparticles as an inorganic ultraviolet (UV)-light absorber can substantially affect the LCN polymer's oscillatory behavior. Our results demonstrate that the oscillation characteristics are directly influenced by the presence of nanoparticles, and we studied the influencing factors. The effectiveness of the elastic modulus, thermomechanical force, and curvature was investigated using different weight percentages of TiO2 nanoparticles. Our results show that, in the presence of TiO2 nanoparticles, the polymer chain order and inter-chain interactions in the polymer matrix as well as the structural deformation of relevant polymer surfaces are changed.
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Human stefin B, a member of the cystatin family of cysteine protease inhibitors, tends to form amyloid fibrils under relatively mild conditions, which is why it is used as a model protein to study amyloid fibrillation. Here, we show for the first time that bundles of amyloid fibrils, i.e., helically twisted ribbons, formed by human stefin B exhibit birefringence. This physical property is commonly observed in amyloid fibrils when stained with Congo red. However, we show that the fibrils arrange in regular anisotropic arrays and no staining is required. They share this property with anisotropic protein crystals, structured protein arrays such as tubulin and myosin, and other anisotropic elongated materials, such as textile fibres and liquid crystals. In certain macroscopic arrangements of amyloid fibrils, not only birefringence is observed, but also enhanced emission of intrinsic fluorescence, implying a possibility to detect amyloid fibrils with no labels by using optical microscopy. In our case, no enhancement of intrinsic tyrosine fluorescence was observed at 303 nm; instead, an additional fluorescence emission peak appeared at 425 to 430 nm. We believe that both phenomena, birefringence and fluorescence emission in the deep blue, should be further explored with this and other amyloidogenic proteins. This may allow the development of label-free detection methods for amyloid fibrils of different origins.
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Amiloide , Cistatinas , Humanos , Cistatina B , Amiloide/metabolismo , Cistatinas/metabolismo , Vermelho Congo , Inibidores de Cisteína ProteinaseRESUMO
We demonstrate the fabrication of good quality surface alignment layers on glass by Direct Laser Writing method using a 2-photon polymerisation technique. We use commercially available photosensitive resins to print alignment layers by scanning the focal point of a femtosecond laser near the resin-glass interface. This results in down to ~ 100 nm thin alignment layers that provide good planar anchoring of 5CB and MLC13300, with the easy axis of alignment along the scanning direction. The azimuthal anchoring strength is ~ 5 × 10-6 J/m2 and is an order of magnitude weaker compared to commercial rubbed polyimide alignment layer. The threshold voltage for Fréedericksz transition in a 90° twisted nematic cell is slightly increased compared to conventional rubbed polyimide for printed alignment layers. The turn-on switching time is longer for printed layers compared to polyimide alignment layers, whereas the turn-off time is shorter for printed alignment layers. The advantage of this new method is in its flexibility, as we demonstrate printing of complex surface alignment patterns with alignment layer thickness below 100 nm.
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Synthetic active matter is emerging as the prime route for the realisation of biological mechanisms such as locomotion, active mixing, and self-organisation in soft materials. In particular, passive nematic complex fluids are known to form out-of-equilibrium states with topological defects, but their locomotion, activation and experimental realization has been developed and understood to only a limited extent. Here, we report that the concentration-driven flow of small molecules triggers turbulent flow in the thin film of a nematic liquid crystal that continuously generates pairs of topological defects with an integer topological charge. The diffusion results in the formation of counter-rotating vortex rolls in the liquid crystal, which above a velocity threshold transform into a turbulent flow with continuous generation and annihilation of the defect pairs. The pairs of defects are created by the self-amplifying splay instability between the vortices, until a pair of oppositely charged defects is formed.
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Liquid crystals (LCs) form an extremely rich range of self-assembled topological structures with artificially or naturally created topological defects. Some of the main applications of LCs are various optical and photonic devices, where compared to their solid-state counterparts, soft photonic systems are fundamentally different in terms of unique properties such as self-assembly, self-healing, large tunability, sensitivity to external stimuli, and biocompatibility. Here we show that complex tunable microlasers emitting structured light can be generated from self-assembled topological LC superstructures containing topological defects inserted into a thin Fabry-Pérot microcavity. The topology and geometry of the LC superstructure determine the structuring of the emitted light by providing complex three-dimensionally varying optical axis and order parameter singularities, also affecting the topology of the light polarization. The microlaser can be switched between modes by an electric field, and its wavelength can be tuned with temperature. The proposed soft matter microlaser approach opens directions in soft matter photonics research, where structured light with specifically tailored intensity and polarization fields could be designed and implemented.
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The shape diversity and controlled reconfigurability of closed surfaces and filamentous structures, universally found in cellular colonies and living tissues, are challenging to reproduce. Here, we demonstrate a method for the self-shaping of liquid crystal (LC) droplets into anisotropic and three-dimensional superstructures, such as LC fibers, LC helices, and differently shaped LC vesicles. The method is based on two surfactants: one dissolved in the LC dispersed phase and the other in the aqueous continuous phase. We use thermal stimuli to tune the bulk LC elasticity and interfacial energy, thereby transforming an emulsion of polydispersed, spherical nematic droplets into numerous, uniform-diameter fibers with multiple branches and vice versa. Furthermore, when the nematic LC is cooled to the smectic-A LC phase, we produce monodispersed microdroplets with a tunable diameter dictated by the cooling rate. Utilizing this temperature-controlled self-shaping of LCs, we demonstrate life-like smectic LC vesicle structures analogous to the biomembranes in living systems. Our experimental findings are supported by a theoretical model of equilibrium interface shapes. The shape transformation is induced by negative interfacial energy, which promotes a spontaneous increase of the interfacial area at a fixed LC volume. The method was successfully applied to many different LC materials and phases, demonstrating a universal mechanism for shape transformation in complex fluids.
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We report a necklace structure made of liquid crystal dispersed in poly(vinyl alcohol) (PVA) aqueous solution, which is fabricated by a microfluidic device. In the necklace structure, liquid crystal droplets that are tens of micrometers in diameter are connected by microtethers, which are birefringent, are not penetrating the droplets, and can be elastically stretched by applying external force. The necklace structure was analyzed by fluorescent confocal microscopy, and the tethers were made of liquid crystal and PVA composite. The elastic constant of the tether was determined by using laser tweezers to stretch the tether. The Whispering Gallery Modes circulating inside individual droplets in the necklace structure were also observed.
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A novel form of nematic gel (N-gel) wherein bright flower-like domains (BFDs) rich in gelator fibres are embedded in a matrix of liquid crystal (LC) molecules has been reported. These gels which we denote as inverse N-gels are unlike typical N-gels in which the LC is encapsulated within an aggregated network of gelator molecules. The self-organization of the helical gelator fibres within the BFDs leads to the creation of localized toron-like structures that are topologically protected due to their skyrmion director profile. Optical and confocal microscopy have been used to deduce the LC director configuration, in order to understand possible intermolecular interactions that can lead to the formation of the twisted structures and the inverse N-gels.
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PURPOSE: The ability to predict an antibody's propensity for aggregation is particularly important during product development to ensure the quality and safety of therapeutic antibodies. We demonstrate the role of container surfaces on the aggregation process of three mAbs under elevated temperature and long-term storage conditions in the absence of mechanical stress. METHODS: A systematic study of aggregation is performed for different proteins, vial material, storage temperature, and presence of surfactant. We use size exclusion chromatography and micro-flow imaging to determine the bulk concentration of aggregates, which we combine with optical and atomic force microscopy of vial surfaces to determine the effect of solid-liquid interfaces on the bulk aggregate concentration under different conditions. RESULTS: We show that protein particles under elevated temperature conditions adhere to the vial surfaces, causing a substantial underestimation of aggregation propensity as determined by common methods used in development of biologics. Under actual long-term storage conditions at 5°C, aggregate particles do not adhere to the surface, causing an increase in bulk concentration of particles, which cannot be predicted from elevated temperature screening tests by common methods alone. We also identify specific protein - surface interactions which promote oligomer formation in the nanometre range. CONCLUSIONS: Special care should be taken when interpreting size exclusion and particle count data from stability studies if different temperatures and vial types are involved. We propose a novel combination of methods to characterise vial surfaces and bulk solution for a full understanding of protein aggregation processes in a sample.
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Produtos Biológicos/química , Anticorpos Monoclonais/química , Química Farmacêutica/métodos , Embalagem de Medicamentos/métodos , Agregados Proteicos , Reprodutibilidade dos Testes , Estresse Mecânico , TemperaturaRESUMO
We used a 16-channel e-nose demonstrator based on micro-capacitive sensors with functionalized surfaces to measure the response of 30 different sensors to the vapours from 11 different substances, including the explosives 1,3,5-trinitro-1,3,5-triazinane (RDX), 1-methyl-2,4-dinitrobenzene (DNT) and 2-methyl-1,3,5-trinitrobenzene (TNT). A classification model was developed using the Random Forest machine-learning algorithm and trained the models on a set of signals, where the concentration and flow of a selected single vapour were varied independently. It is demonstrated that our classification models are successful in recognizing the signal pattern of different sets of substances. An excellent accuracy of 96% was achieved for identifying the explosives from among the other substances. These experiments clearly demonstrate that the silane monolayers used in our sensors as receptor layers are particularly well suited to selecting and recognizing TNT and similar types of explosives from among other substances.
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Skyrmions are swirl-like topological entities that have been shown to emerge in various condensed matter systems. Their identification has been carried out in different ways including scattering techniques and real-space observations. Here we show that Kossel diagrams can identify the formation of a hexagonal lattice of half-Skyrmions in a thin film of a chiral liquid crystal, in which case Kossel lines appear as hexagonally arranged circular arcs. Our experimental observations on a hexagonal lattice of half-Skyrmions and other defect structures resembling that of a bulk cholesteric blue phase are perfectly accounted for by numerical calculations and a theoretical argument attributing strong reflections yielding Kossel lines to guided mode resonances in the thin liquid crystal film. Our study demonstrates that a liquid crystal is a model system allowing the investigation of topological entities by various optical means, and also that Kossel techniques are applicable to the investigation of thin systems with non-trivial photonic band structures including topologically protected optical surface states.
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We demonstrate how the geometric shape of a rod in a nematic liquid crystal can stabilise a large number of oppositely charged topological defects. A rod is of the same shape as a sphere, both having genus g = 0, which means that the sum of all topological charges of defects on a rod has to be -1 according to the Gauss-Bonnet theorem. If the rod is straight, it usually shows only one hyperbolic hedgehog or a Saturn ring defect with negative unit charge. Multiple unit charges can be stabilised either by friction or large length, which screens the pair-interaction of unit charges. Here we show that the curved shape of helical colloids or the grooved surface of a straight rod create energy barriers between neighbouring defects and prevent their annihilation. The experiments also clearly support the Gauss-Bonnet theorem and show that topological defects on helices or grooved rods always appear in an odd number of unit topological charges with a total topological charge of -1.
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We demonstrate non-contact temperature measurement with one tenth of a kelvin precision at distances of several meters using omnidirectional laser emission from dye-doped cholesteric liquid crystal droplets freely floating in a fluid medium. Upon the excitation with a pulsed laser the liquid crystal droplet emits laser light due to 3D Bragg lasing in all directions. The spectral position of the lasing is highly dependent on temperature, which enables remote and contact-less temperature measurement with high precision. Both laser excitation and collection of light emitted by microlasers is performed through a wide telescope aperture optics at a distance of up to several meters. The optical excitation volume, where the droplets are excited and emitting the laser light is of the order of ten cubic millimeters. The measurement is performed with ten second accumulation time, when several droplets pass through the excitation volume due to their motion. The time of measurement could easily be shortened to less than a second by increasing the rate of the excitation laser. Since the method is based solely on measuring the spectral position of a single and strong laser line, it is quite insensitive to scattering, absorption and background signals, such as autofluorescence. This enables a wide use in science and industry, with a detection range exceeding tens of meters.
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Although the link between the inhalation of nanoparticles and cardiovascular disease is well established, the causal pathway between nanoparticle exposure and increased activity of blood coagulation factors remains unexplained. To initiate coagulation tissue factor bearing epithelial cell membranes should be exposed to blood, on the other side of the less than a micrometre thin air-blood barrier. For the inhaled nanoparticles to promote coagulation, they need to bind lung epithelial-cell membrane parts and relocate them into the blood. To assess this hypothesis, we use advanced microscopy and spectroscopy techniques to show that the nanoparticles wrap themselves with epithelial-cell membranes, leading to the membrane's disruption. The membrane-wrapped nanoparticles are then observed to freely diffuse across the damaged epithelial cell layer relocating epithelial cell membrane parts over the epithelial layer. Proteomic analysis of the protein content in the nanoparticles wraps/corona finally reveals the presence of the coagulation-initiating factors, supporting the proposed causal link between the inhalation of nanoparticles and cardiovascular disease.
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Membrana Celular/metabolismo , Células Epiteliais/metabolismo , Nanotubos/química , Titânio/química , Animais , Coagulação Sanguínea/fisiologia , Movimento Celular , Sobrevivência Celular , Humanos , Bicamadas Lipídicas/química , Bicamadas Lipídicas/metabolismo , Pulmão/citologia , Camundongos , Tamanho da Partícula , Coroa de Proteína/metabolismo , Proteoma/metabolismo , Transdução de Sinais , Propriedades de SuperfícieRESUMO
Nowadays, complicated topological defects enable many experimental manipulations and configurational simulations of active soft matter for optical and photonic applications. Investigation of topological defects in soft anisotropic materials enables one to better understand three-dimensional orientation fields in cholesteric liquid crystals. Here, we describe optical properties of bistable bubble domain (BD) texture torons in a thin layer of cholesteric liquid crystal (CLC), frustrated by homeotropic anchoring conditions, and reliably switchable by a random process. The control of macroscopic optical density and diffraction efficiency of the BD texture is demonstrated by a selection of a confinement ratio of the CLC. Experimentally reconstructed CLC director profile reveals the topology of BD torons allowing consideration of naturally occurring BD texture for applications in optical and photonic devices, which are bistably switchable between active and transparent optical states.
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Topology has an increasingly important role in the physics of condensed matter, quantum systems, material science, photonics and biology, with spectacular realizations of topological concepts in liquid crystals. Here we report on long-lived hidden topological states in thermally quenched, chiral nematic droplets, formed from string-like, triangular and polyhedral constellations of monovalent and polyvalent singular point defects. These topological defects are regularly packed into a spherical liquid volume and stabilized by the elastic energy barrier due to the helical structure and confinement of the liquid crystal in the micro-sphere. We observe, for the first time, topological three-dimensional point defects of the quantized hedgehog charge q=-2, -3. These higher-charge defects act as ideal polyvalent artificial atoms, binding the defects into polyhedral constellations representing topological molecules.
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We report magnetic field tuning of the structure and Whispering Gallery Mode lasing from ferromagnetic nematic liquid crystal micro-droplets. Microlasers were prepared by dispersing a nematic liquid crystal, containing magnetic nanoparticles and fluorescent dye, in a glycerol-lecithin matrix. The droplets exhibit radial director structure, which shows elastic distortion at a very low external magnetic field. The fluorescent dye doped ferromagnetic nematic droplets show Whispering Gallery Mode lasing, which is tunable by the external magnetic field. The tuning of the WGM lasing modes is linear in magnetic field with a wavelength-shift of the order of 1 nm/100 mT. Depending on the lasing geometry, the WGMs are red- or blue-shifted.
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This article provides a concise review of a new state of colloidal matter called nematic liquid-crystal colloids. These colloids are obtained by dispersing microparticles of different shapes in a nematic liquid crystal that acts as a solvent for the dispersed particles. The microparticles induce a local deformation of the liquid crystal, which then generates topological defects and long-range forces between the neighboring particles. The colloidal forces in nematic colloids are much stronger than the forces in ordinary colloids in isotropic solvents, exceeding thousands of kBT per micrometer-sized particle. Of special interest are the topological defects in nematic colloids, which appear in many fascinating forms, such as singular points, closed loops, multitudes of interlinked and knotted loops or soliton-like structures. The richness of the topological phenomena and the possibility to design and control topological defects with laser tweezers make colloids in nematic liquid crystals an excellent playground for testing the basic theorems of topology.
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Good chemical selectivity of sensors for detecting vapour traces of targeted molecules is vital to reliable detection systems for explosives and other harmful materials. We present the design, construction and measurements of the electronic response of a 16 channel electronic nose based on 16 differential microcapacitors, which were surface-functionalized by different silanes. The e-nose detects less than 1 molecule of TNT out of 10+12 N2 molecules in a carrier gas in 1 s. Differently silanized sensors give different responses to different molecules. Electronic responses are presented for TNT, RDX, DNT, H2S, HCN, FeS, NH3, propane, methanol, acetone, ethanol, methane, toluene and water. We consider the number density of these molecules and find that silane surfaces show extreme affinity for attracting molecules of TNT, DNT and RDX. The probability to bind these molecules and form a surface-adsorbate is typically 10+7 times larger than the probability to bind water molecules, for example. We present a matrix of responses of differently functionalized microcapacitors and we propose that chemical selectivity of multichannel e-nose could be enhanced by using artificial intelligence deep learning methods.