RESUMO
Heat stress is a major problem that constrains pig productivity. Understanding and identifying adaptation to heat stress has been the focus of recent studies, and the identification of genome-wide selection signatures can provide insights into the mechanisms of environmental adaptation. Here, we generated whole-genome re-sequencing data from six Chinese indigenous pig populations to identify genomic regions with selection signatures related to heat tolerance using multiple methods: three methods for intra-population analyses (Integrated Haplotype Score, Runs of Homozygosity and Nucleotide diversity Analysis) and three methods for inter-population analyses (Fixation index (FST), Cross-population Composite Likelihood Ratio and Cross-population Extended Haplotype Homozygosity). In total, 1 966 796 single nucleotide polymorphisms were identified in this study. Genetic structure analyses and FST indicated differentiation among these breeds. Based on information on the location environment, the six breeds were divided into heat and cold groups. By combining two or more approaches for selection signatures, outlier signals in overlapping regions were identified as candidate selection regions. A total of 163 candidate genes were identified, of which, 29 were associated with heat stress injury and anti-inflammatory effects. These candidate genes were further associated with 78 Gene Ontology functional terms and 30 Kyoto Encyclopedia of Genes and Genomes pathways in enrichment analysis (P < 0.05). Some of these have clear relevance to heat resistance, such as the AMPK signalling pathway and the mTOR signalling pathway. The results improve our understanding of the selection mechanisms responsible for heat resistance in pigs and provide new insights of introgression in heat adaptation.
Assuntos
Termotolerância , Suínos/genética , Animais , Seleção Genética , Genoma , Homozigoto , Haplótipos , Polimorfismo de Nucleotídeo ÚnicoRESUMO
1. The objective of this study was to reveal the role of chicken RB1 (Gallus gallus RB1, gRB1) in the proliferation of preadipocytes. 2. To measure gene expression of gRB1 in the proliferation of chicken preadipocyte, quantitative real-time PCR was used. The expression levels of gRB1 transiently increased during this process. 3. To detect the effect of gRB1 on the proliferation of chicken preadipocyte, MTT assay and cell-cycle analysis were performed. MTT assay showed that overexpression of gRB1 significantly suppressed (P < 0.05) the proliferation of chicken preadipocytes, and knockdown of gRB1 promoted the proliferation of chicken preadipocytes. Cell-cycle analysis showed that the proportion of preadipocytes in the G1 and G2 phases significantly increased (P < 0.05), and the proportion of preadipocytes in the S phase significantly decreased (P < .05) after up-regulation of the expression of gRB1. The proportion of preadipocytes in the S phase significantly increased (P < 0.05) after down-regulation of gRB1. 4. Quantitative real-time PCR was used to detect the effect of gRB1 on the expression of genes related to proliferation of chicken preadipocytes. Gene expression analysis showed that gRB1 knockdown promoted markers indicating proliferation of Ki-67 (MKi67) expression at 96 h (P < 0.05), and overexpression of gRB1 reduced MKi67 expression at 72 h (P < 0.05). 5. This study demonstrated that gRB1 inhibited preadipocyte proliferation at least in part by inhibiting the G1 to S phase transition.
Assuntos
Adipócitos/fisiologia , Proteínas Aviárias/genética , Proliferação de Células/genética , Galinhas/fisiologia , Fator de Transcrição E2F1/genética , Animais , Proteínas Aviárias/metabolismo , Fator de Transcrição E2F1/metabolismo , Reação em Cadeia da Polimerase em Tempo RealRESUMO
Since the emergence of highly pathogenic avian influenza (HPAI) H5N1 in Asia, the haemagglutinin (HA) gene of this virus lineage has continued to evolve in avian populations, and H5N1 lineage viruses now circulate concurrently worldwide. Dogs may act as an intermediate host, increasing the potential for zoonotic transmission of influenza viruses. Virus transmission and pathologic changes in HPAI clade 1.1.2 (H5N1)-, 2.3.2.1c (H5N1)- and 2.3.4.4 (H5N6)-infected dogs were investigated. Mild respiratory signs and antibody response were shown in dogs intranasally infected with the viruses. Lung histopathology showed lesions that were associated with moderate interstitial pneumonia in the infected dogs. In this study, HPAI H5N6 virus replication in dogs was demonstrated for the first time. Dogs have been suspected as a "mixing vessel" for reassortments between avian and human influenza viruses to occur. The replication of these three subtypes of the H5 lineage of HPAI viruses in dogs suggests that dogs could serve as intermediate hosts for avian-human influenza virus reassortment if they are also co-infected with human influenza viruses.
Assuntos
Doenças do Cão/virologia , Vírus da Influenza A/fisiologia , Infecções por Orthomyxoviridae/veterinária , Replicação Viral , Animais , Doenças do Cão/patologia , Cães , Virus da Influenza A Subtipo H5N1/classificação , Virus da Influenza A Subtipo H5N1/fisiologia , Vírus da Influenza A/classificação , Infecções por Orthomyxoviridae/patologia , Infecções por Orthomyxoviridae/virologiaRESUMO
Bat species around the world have recently been recognized as major reservoirs of several zoonotic viruses, such as severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), Nipah virus and Hendra virus. In this study, consensus primer-based reverse transcriptase polymerase chain reactions (RT-PCRs) and high-throughput sequencing were performed to investigate viruses in bat faecal samples collected at 11 natural bat habitat sites from July to December 2015 in Korea. Diverse coronaviruses were first detected in Korean bat faeces, including alphacoronaviruses, SARS-CoV-like and MERS-CoV-like betacoronaviruses. In addition, we identified a novel bat rotavirus belonging to group H rotavirus which has only been described in human and pigs until now. Therefore, our results suggest the need for continuing surveillance and additional virological studies in domestic bat.
Assuntos
Quirópteros/virologia , Coronavirus/isolamento & purificação , Fezes/virologia , Rotavirus/isolamento & purificação , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave/isolamento & purificação , Animais , República da CoreiaRESUMO
OBJECTIVE: To investigate the effect of Iptakalim (Ipt) preventing injury of endothelial microvesicles (EMVs) derived from hypoxia/reoxygenation (H/R)-treated HUVECs on the relaxation of rat thoracic aortic rings and explore the underlying mechanism. METHODS: H/R injury model was established to release H/R-EMVs from HUVECs. H/R-EMVs from HUVECs were isolated by ultracentrifugation from the conditioned culture medium. H/R-EMVs were characterized by using Transmission Electron Microscope (TEM). Thoracic aortic rings of rats were incubated with 10-7-10-3 mol/L Ipt and co-cultured with 10 µg/ml H/R-EMVs for 4 hours, and their endothelium-dependent relaxation in response to acetylcholine (ACh) was recorded in vitro. The nitric oxide (NO) production of ACh-treated rat thoracic aortic rings was measured by using Griess reagent. The expression of endothelial NO synthase (eNOS), phosphorylated eNOS (p-eNOS, Ser-1177), serine/threonine kinas (Akt) and phosphorylated Akt (p-Akt, Ser-473) in the thoracic aortic rings of rats was detected by Western blotting. RESULTS: H/R-EMVs were induced by H/R-treated HUVECs and isolated by ultracentrifugation. The isolated H/R-EMVs subjected to TEM revealed small, rounded vesicles (100-1 000 nm) surrounded by a membrane. H/R-EMVs impaired relaxation induced by ACh of rat thoracic aortic rings significantly. Compared with H/R-EMVs treatment individually, relaxation and NO production of rat thoracic aortic rings were increased by Ipt treatment in a concentration-dependent manner (P<0.05, P<0.01). The expression of total eNOS (t-eNOS) and total Akt (t-Akt) was not affected by Ipt or H/R-EMVs. However, the expression of p-eNOS and p-Akt increased after treated with Ipt (P<0.01). CONCLUSIONS: Based on H/R-EMVs treatment, ACh induced endothelium-dependent relaxation of rat thoracic aortic rings was ameliorated by Ipt in a concentration-dependent manner. The mechanisms involved the increase in NO production, p-eNOS and p-Akt expression.
Assuntos
Acetilcolina/farmacologia , Aorta Torácica/efeitos dos fármacos , Micropartículas Derivadas de Células , Propilaminas/farmacologia , Vasodilatação/efeitos dos fármacos , Animais , Hipóxia Celular , Células Endoteliais da Veia Umbilical Humana , Humanos , Técnicas In Vitro , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo III/metabolismo , RatosRESUMO
BACKGROUND: The pandemic strain of the influenza A virus (pH1N1) in 2009 caused many complications in patients. In this study, we introduce asthmatic symptoms as a complication of pH1N1 infection in children, not having a relationship with asthma history. The aim of this study was to quantify asthmatic symptoms in pH1N1-infected children and elucidate the underlying mechanisms of airway hyper-responsiveness (AHR) induced in a murine model of pH1N1 infection. METHODS: As a retrospective study, pH1N1-infected children who were hospitalized with moderate to severe acute asthmatic symptoms were enrolled and administered a methacholine challenge test (MCT) at 3 months post-discharge. Additionally, the induction of AHR by pH1N1 infection was measured by MCT in wild-type and Rag1(-/-) mice. The effect of the innate immune response on the development of AHR following pH1N1 infection was investigated. RESULTS: More than 70% of the pH1N1-infected children without a pre-infection diagnosis of asthma had a negative response on the MCT. None of these children had recurrent wheezing or asthma during the 3 years following pH1N1 infection. The development of AHR in pH1N1-infected mice was associated with an elevation in IL-33 and innate lymphoid cells 2 (ILC2). CONCLUSIONS: This study demonstrates that pH1N1 infection directly induces transient asthmatic symptoms in patients regardless of their medical history. pH1N1 infection was shown to stimulate the rapid development of AHR and Th2-type cytokine secretion in mice via the activation of ILC2; it may be activated independently of adaptive immunity.
Assuntos
Vírus da Influenza A Subtipo H1N1/imunologia , Influenza Humana/imunologia , Linfócitos/imunologia , Infecções por Orthomyxoviridae/imunologia , Pandemias , Adolescente , Animais , Asma/imunologia , Criança , Pré-Escolar , Feminino , Humanos , Imunidade Inata , Influenza Humana/epidemiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Estudos RetrospectivosRESUMO
This study investigated the role of the chicken liver fatty acid-binding protein (L-FABP) gene in lipid metabolism in hepatocytes, and the regulatory relationships between L-FABP and genes related to lipid metabolism. The short hairpin RNA (shRNA) interference vector with L-FABP and an eukaryotic expression vector were used. Chicken hepatocytes were subjected to shRNA-mediated knockdown or L-FABP cDNA overexpression. Expression levels of lipid metabolism-related genes and biochemical parameters were detected 24, 36, 48, 60, and 72 h after transfection with the interference or overexpression plasmids for L-FABP, PPARα and L-BABP expression levels, and the total amount of cholesterol, were significantly affected by L-FABP expression. L-FABP may affect lipid metabolism by regulating PPARα and L-BABP in chicken hepatocytes.
Assuntos
Galinhas/genética , Proteínas de Ligação a Ácido Graxo/genética , Hepatócitos/metabolismo , Metabolismo dos Lipídeos/genética , Animais , Galinhas/metabolismo , Proteínas de Ligação a Ácido Graxo/antagonistas & inibidores , Regulação da Expressão Gênica , PPAR alfa/genética , RNA Interferente PequenoRESUMO
In this study, we profiled gene expression in chicken liver and screened differentially expressed genes in the Bai'er layers and fat line broilers. Birds were derived from the 14th generation of Northeast Agricultural University fat broiler lines and Bai'er layers. Chicken genome arrays were used to screen differentially expressed genes in liver tissue from the Bai'er layers and fat line broilers. We screened 671 differentially expressed genes between broilers and layers at the ages of 2 and 4 weeks. We observed enrichment of a series of significant pathways, including the mitogen-activated protein kinase signaling pathway, cell cycle, mammalian target of rapamycin signaling pathway, and p53 signaling pathway. At 2 and 4 weeks, 94 shared differentially expressed genes were observed. We speculated that these genes regulate chicken lipid metabolism.
Assuntos
Galinhas/genética , Perfilação da Expressão Gênica/métodos , Fígado/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Fatores Etários , Animais , Galinhas/classificação , Metabolismo dos Lipídeos/genética , Transdução de Sinais/genética , Especificidade da EspécieRESUMO
After an outbreak of pandemic influenza A/H1N1 (pH1N1) virus, we had previously reported the emergence of a recombinant canine influenza virus (CIV) between the pH1N1 virus and the classic H3N2 CIV. Our ongoing routine surveillance isolated another reassortant H3N2 CIV carrying the matrix gene of the pH1N1 virus from 2012. The infection dynamics of this H3N2 CIV variant (CIV/H3N2mv) were investigated in dogs and ferrets via experimental infection and transmission. The CIV/H3N2mv-infected dogs and ferrets produced typical symptoms of respiratory disease, virus shedding, seroconversion, and direct-contact transmissions. Although indirect exposure was not presented for ferrets, CIV/H3N2mv presented higher viral replication in MDCK cells and more efficient transmission was observed in ferrets compared to classic CIV H3N2. This study demonstrates the effect of reassortment of the M gene of pH1N1 in CIV H3N2.
Assuntos
Doenças do Cão/virologia , Furões/virologia , Vírus da Influenza A Subtipo H1N1/genética , Infecções por Orthomyxoviridae/veterinária , Pandemias/veterinária , Animais , Sequência de Bases , Doenças do Cão/epidemiologia , Doenças do Cão/transmissão , Cães/virologia , Genes Virais/genética , Vírus da Influenza A Subtipo H3N2/genética , Vírus da Influenza A Subtipo H3N2/isolamento & purificação , Células Madin Darby de Rim Canino/virologia , Dados de Sequência Molecular , Infecções por Orthomyxoviridae/epidemiologia , Infecções por Orthomyxoviridae/genética , Infecções por Orthomyxoviridae/transmissão , Pandemias/estatística & dados numéricos , Recombinação Genética/genética , Proteínas da Matriz Viral/genéticaRESUMO
This study aimed to investigate gene expression in the chicken liver for lean and fat broiler lines. Birds used in this study were 2 and 4 weeks of age; they were derived from the 14th generation of Northeast Agricultural University broiler lines, which were divergently selected based on abdominal fat content. Chicken Genome Arrays were used to screen differentially expressed genes in the liver tissue from lean and fat birds. At 2 and 4 weeks of age, 770 and 452 genes were differentially expressed between the 2 lines, respectively. The differentially expressed genes were involved in Wnt, insulin signaling, and cell cycle pathways. At 2 and 4 weeks, 42 shared, differentially expressed genes were revealed by the analysis. We speculate that these genes might regulate chicken lipid metabolism.
Assuntos
Galinhas/genética , Redes Reguladoras de Genes , Metabolismo dos Lipídeos , Fígado/metabolismo , Animais , Ciclo Celular , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Insulina/genética , Fígado/patologia , Análise de Sequência com Séries de Oligonucleotídeos , Via de Sinalização WntRESUMO
We aimed to investigate the association of inflammation-related genes such as IL-10, IL-6 and IL-1B with risk of ischemic stroke. We included 426 cases with ischemic stroke and 426 health controls from Xinxiang, China. Genomic DNA was extracted from the buffy coat layer of collected blood with the TIANamp blood DNA kit. Diabetes, hypertension, obesity, and smoking habits were associated with risk of ischemic stroke. We found that individuals carrying the CC genotype of IL-1B rs1864169 had a higher risk of ischemic stroke when compared with the TT genotype (OR = 1.80, 95%CI = 1.16-2.80). The IL-6 rs1800796 TT genotype was associated with increased risk of ischemic stroke. We found that IL-1B rs1864169 and IL-6 rs1800796 polymorphisms may interact with diabetes, hypertension and obesity. Our study suggests that IL-6 rs1800796 and IL-1B rs1864169 polymorphisms are associated with ischemic stroke risk in the Chinese population.
Assuntos
Isquemia Encefálica/genética , Interleucina-10/genética , Interleucina-1beta/genética , Interleucina-6/genética , Trombose Intracraniana/genética , Acidente Vascular Cerebral/genética , Adulto , Povo Asiático , Isquemia Encefálica/sangue , Isquemia Encefálica/etnologia , Isquemia Encefálica/patologia , Estudos de Casos e Controles , Diabetes Mellitus/fisiopatologia , Feminino , Genótipo , Humanos , Hipertensão/fisiopatologia , Interleucina-10/sangue , Interleucina-1beta/sangue , Interleucina-6/sangue , Trombose Intracraniana/sangue , Trombose Intracraniana/etnologia , Trombose Intracraniana/patologia , Masculino , Pessoa de Meia-Idade , Obesidade/fisiopatologia , Polimorfismo de Nucleotídeo Único , Fatores de Risco , Fumar/fisiopatologia , Acidente Vascular Cerebral/sangue , Acidente Vascular Cerebral/etnologia , Acidente Vascular Cerebral/patologiaRESUMO
Equine influenza virus (EIV) causes a highly contagious respiratory disease in equids, with confirmed outbreaks in Europe, America, North Africa, and Asia. Although China, Mongolia, and Japan have reported equine influenza outbreaks, Korea has not. Since 2011, we have conducted a routine surveillance programme to detect EIV at domestic stud farms, and isolated H3N8 EIV from horses showing respiratory disease symptoms. Here, we characterized the genetic and biological properties of this novel Korean H3N8 EIV isolate. This H3N8 EIV isolate belongs to the Florida sublineage clade 1 of the American H3N8 EIV lineage, and surprisingly, possessed a non-structural protein (NS) gene segment, where 23 bases of the NS1-encoding region were naturally truncated. Our preliminary biological data indicated that this truncation did not affect virus replication; its effect on biological and immunological properties of the virus will require further study.
Assuntos
Vírus da Influenza A Subtipo H3N8/isolamento & purificação , Infecções por Orthomyxoviridae/veterinária , Infecções por Orthomyxoviridae/virologia , Proteínas não Estruturais Virais/genética , Animais , Sequência de Bases , Cães , Cavalos , Vírus da Influenza A Subtipo H3N8/classificação , Vírus da Influenza A Subtipo H3N8/genética , Células Madin Darby de Rim Canino , Dados de Sequência Molecular , Cavidade Nasal/virologia , Filogenia , República da Coreia , Cultura de Vírus , Replicação ViralRESUMO
X-ray repair cross complementing 1 (XRCC1) protein plays an important role in base excision repair. Single nucleotide polymorphisms (SNPs) in XRCC1 gene may affect DNA repairing ability and genetic susceptibility to cancer. This study was designed to investigate the correlation of XRCC1 Arg194Trp Arg280His and Arg399Gln SNPs with the risk of gastric cardiac adenocarcinoma (GCA). Genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism assay in 455 patients with GCA and 650 age and sex-matched controls. We did not find any significant difference in allele and genotype distributions of Arg194Trp Arg399Gln between the groups (P > 0.05). However, a significant increase in GCA risk was seen among smokers if they carried at least one XRCC1 280His (Arg280His + His280His) genotype (odds ratio = 1.59, 95%confidence interval = 1.01-2.51) compared with smokers not carrying these genotype. Our results indicated that XRCC1 Arg194Trp and Arg399Gln SNPs might not be associated with the risk of GCA. However, smokers with His allele at codon 280 had a significantly increased risk of GCA.
Assuntos
Adenocarcinoma/genética , Cárdia/patologia , Reparo do DNA/genética , Proteínas de Ligação a DNA/genética , Polimorfismo de Nucleotídeo Único/genética , Neoplasias Gástricas/genética , Adenina , Adulto , Idoso , Arginina/genética , Estudos de Casos e Controles , Códon/genética , Citosina , Feminino , Frequência do Gene/genética , Predisposição Genética para Doença/genética , Genótipo , Glutamina/genética , Haplótipos , Histidina/genética , Humanos , Desequilíbrio de Ligação/genética , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Fumar/genética , Timina , Triptofano/genética , Proteína 1 Complementadora Cruzada de Reparo de Raio-XRESUMO
AIMS: To investigate the effect of the yeast-conform variant of the Artemisia annua gene encoding for amorpha-4,11-diene synthase (ADS) on the production of amorpha-4,11-diene in a transformed yeast. METHODS AND RESULTS: The ADS gene was mutated to the yeast-conform variant ADSm. The ADSm synthesis was performed based on step-by-step extension of a short region of the gene through a series of polymerase chain reactions (PCR). The artificial ADSm gene contained codons preferred by the yeast translation machinery. The sequence was then integrated into a yeast expression vector pYeDP60. The fusion construct was active and the transformed yeast cells produced higher level of amorpha-4,11-diene compared with the plant gene-transformed yeast cells. CONCLUSIONS: Strains transformed with the yeast-conform allele (ADSm) were more efficient in terms of production of amorpha-4,11-diene than those transformed with the plant gene. SIGNIFICANCE AND IMPACT OF THE STUDY: We demonstrated that yeast-conform allele of foreign genes by serial PCR reactions can be a solution to low efficiency of heterologous gene expression in Saccharomyces cerevisiae cells.
Assuntos
Alquil e Aril Transferases/metabolismo , Artemisia annua/enzimologia , Engenharia Genética/métodos , Saccharomyces cerevisiae/genética , Sesquiterpenos/metabolismo , Alquil e Aril Transferases/genética , Artemisia annua/genética , Artemisia annua/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Genoma Fúngico/genética , Dados de Sequência Molecular , Plasmídeos/genética , Sesquiterpenos Policíclicos , Reação em Cadeia da Polimerase , Recombinação Genética , Saccharomyces cerevisiae/metabolismo , Transformação GenéticaRESUMO
A 63-year-old man presented with a dumbbell-shaped mass obstructing his left upper lobe. He underwent a left upper lobectomy for suspected malignancy. An unexpected pathological diagnosis of endobronchial lipomatous hamartoma was made. Only 6 other cases have been reported in the English literature and only two cases were both endobronchial and exophytic.
Assuntos
Broncopatias/patologia , Hamartoma/patologia , Lipomatose/patologia , Broncopatias/cirurgia , Hamartoma/cirurgia , Humanos , Lipomatose/cirurgia , Masculino , Pessoa de Meia-Idade , Pneumonectomia , Toracotomia , Tomografia Computadorizada por Raios X , Resultado do TratamentoRESUMO
p16 is an important tumor suppressor gene, which is inactivated in many kinds of tumors. The common variants of p16 may be associated with the risk of certain tumors development. We analyzed the frequency of two adjacent polymorphisms in p16 exon 3 (540C-->G and 580C-->T) and their haplotype in blood samples from epithelial ovarian cancer (EOC) patients and healthy controls using polymerase chain reaction-restriction fragment length polymorphism. The results showed that the genotype frequency of p16 580C-->T polymorphism was significantly different among histologic subtypes of EOC (P= 0.02). T allele carriers significantly reduced the risk of serous EOC; the adjusted odds ratio was 0.40 (95% CI = 0.19-0.84). There are neither association between p16 540C-->G polymorphism and EOC development, progression, nor association between the haplotypes of two single nucleotide polymorphisms and the tumor development. Our results suggested that the p16 580C-->T polymorphism might affect the individual susceptibility to specific subtypes of EOC. Different types of ovarian cancer might adopt distinct carcinogenetic pathways. However, this result may be further validated in a larger sample of patients.
Assuntos
Adenocarcinoma Mucinoso/genética , Carcinoma Endometrioide/genética , Inibidor p16 de Quinase Dependente de Ciclina/genética , Cistadenocarcinoma Seroso/genética , Neoplasias Ovarianas/genética , Polimorfismo de Nucleotídeo Único/genética , Regiões 3' não Traduzidas/genética , Adenocarcinoma Mucinoso/metabolismo , Adenocarcinoma Mucinoso/patologia , Adulto , Idoso , Carcinoma Endometrioide/metabolismo , Carcinoma Endometrioide/patologia , Estudos de Casos e Controles , China , Cistadenocarcinoma Seroso/metabolismo , Cistadenocarcinoma Seroso/patologia , Progressão da Doença , Feminino , Predisposição Genética para Doença , Genótipo , Haplótipos/genética , Humanos , Pessoa de Meia-Idade , Neoplasias Ovarianas/metabolismo , Neoplasias Ovarianas/patologia , Reação em Cadeia da PolimeraseRESUMO
Sulfite was detected in 7 varieties of Oriental herbal medicines (Pueraria radix, Zingiberis rhizoma, Platycodon radix, Adenophora radix, Pinellia tuber, Astragalus radix, and Paeonia radix) on the Korean market. Sulfiting of commercial Oriental herbal medicines by fumigation with burning bituminous coal was simulated, and the accumulation of sulfite was investigated by using fresh Platycodon radix roots obtained from a growing field. The sulfite level reached a plateau in 9 h, and the maximum sulfite level found by the Monier-Williams (MW) method (AOAC 990.28) was 1020 ppm. The sulfite content in the simulated Platycodon radix sample determined by alkali extraction followed by ion-exclusion chromatography with electrochemical detection (AOAC 990.31) was approximately 17% lower on average than the MW results. Free-sulfite levels determined by acid extraction and ion-exclusion chromatography with electrochemical detection were between 19 and 49% of the MW results. The advantages of different methods for sulfite determination and the significance of the results are discussed.
Assuntos
Medicamentos de Ervas Chinesas/análise , Sulfitos/análise , Cromatografia por Troca Iônica , Eletroquímica , Indicadores e Reagentes , Plantas Medicinais/químicaRESUMO
PURPOSE: The aim of this study was to present the technique of megasigmoid resection and anal reconstruction by complete posterior sagittal approach for the children with severe constipation and fecal incontinence after anoplasty. METHODS: Six patients (age, 2 to 18 years) born with imperforate anus and originally treated with perineal anoplasty suffered from intractable constipation and fecal incontinence. Contrast enema showed massive dilated and aperistaltic rectosigmoid colon with fecal impaction. Resection of the dilated bowel and anal reconstruction were completely performed by posterior sagittal approach. RESULTS: The mean operating time was 205 minutes (range, 125 to 265 minutes) and the average length of resected colon was 23.3 cm (range, 10 to 40 cm). There were no intraoperative or postoperative complications. By 2 to 4 months after the operation, all patients obtained voluntary bowel movement. On follow-up at 6 to 24 months postoperative, no patient had constipation or required use of the laxatives again. Four of 6 patients suffered from grade 1 soiling, and the other 2 had grade greater than 1 soiling. None had urinary retention or incontinence after the procedure. CONCLUSION: Resection of dilated rectosigmoid colon and anal reconstruction for the patients with severe constipation and fecal incontinence after anoplasty can be performed successfully using a posterior sagittal approach.
Assuntos
Colo Sigmoide/cirurgia , Constipação Intestinal/cirurgia , Incontinência Fecal/cirurgia , Complicações Pós-Operatórias/cirurgia , Adolescente , Criança , Pré-Escolar , Procedimentos Cirúrgicos do Sistema Digestório , Feminino , Humanos , Masculino , Índice de Gravidade de DoençaRESUMO
BACKGROUND: An animal model demonstrating high metastasis of human prostate carcinoma is of importance in studying the biology and therapy of human prostate carcinoma Dr. Hoffman's group recently used surgical orthotopic implantation (SOI) of human prostate cells to nude mice to establish an animal model with high metastatic activity. To confirm the animal model by SOI reproducible in other laboratories and shorten the period requiring for the metastasis, we adopted SOI technique with a modification using PC-3M human prostate cell line which showed a higher metastatic activity than PC-3. MATERIALS AND METHODS: Intact tissue of the human prostate carcinoma cell line PC-3M was prepared by growth of this cell subcutaneous in a nude mouse. One piece of 1.5 mm3 intact tumor tissue was implanted by orthotopical surgery to the ventral lateral lobes of the prostate gland of 10 nude mice. Mice were sacrificed when they were found to be moribund. Metastasis in other tissues was evaluated by gross and microscopic morphology. RESULTS: All 10 mice showed the tumorigenesis in the prostate gland and metastasis of human prostate tumor cells into periaortic lymph nodes without other organ's metastasis. The time when mice with PC-3M SOI start moribund is 28-32 days after SOI. CONCLUSIONS: SOI is good technique to establish the efficiently metastatic animal mode. SOI using PC-3M human prostate cell line will leads to 100% metastasis of prostate tumor cells. So far, this model is much quicker and more efficient than those reported in literatures.