Administration of amyloid-ß oligomer to the buccal ganglia may reduce food intake and cholinergic synaptic responses within the feeding neural circuit in Aplysia kurodai.

Anorexia is a behavioral change caused by functional brain disorders in patients with Alzheimer's disease (AD). Amyloid-ß (1-42) oligomers (o-Aß) are possible causative agents of AD that impair signaling via synaptic dysfunction. In this study, we used Aplysia kurodai to study functional disorders of the brain through o-Aß. Administration of o-Aß to the buccal ganglia (feeding brain for oral movements) by surgical treatment significantly reduced food intake for at least five days. Furthermore, we explored the effects of o-Aß on the synaptic function in the feeding neural circuit, focusing on a specific inhibitory synaptic response in jaw-closing motor neurons produced by cholinergic buccal multi-action neurons because we recently found that this cholinergic response decreases with aging, which is consistent with the cholinergic hypothesis for aging. Administration of o-Aß to the buccal ganglia significantly reduced the synaptic response within minutes, whereas administration of amyloid-ß (1-42) monomers did not. These results suggest that o-Aß may impair the cholinergic synapses, even in Aplysia, which is consistent with the cholinergic hypothesis for AD.

An age-related decline in the cholinergic synaptic response may cause the firing pattern in the jaw-closing motor neurons, which resembles the aversive taste response in the feeding behavior of old Aplysia kurodai.

Anorexia due to aging is recognized as a syndrome of animal feeding behavior. Age-related functional disorders of the brain often cause behavioral changes. We used Aplysia kurodai to study this neural mechanism, following our previous study on food preference behaviors. The age of each wild animal was defined by a previously described method, and a significant age-related decline in food intake was observed. In this study, we explored the effects of aging on a specific inhibitory synaptic response in jaw-closing (JC) motor neurons produced by cholinergic multiaction (MA) neurons, the size of which determines the delay between MA and JC firings and this delay is reduced during aversive taste responses; in our analyses, we found a significant age-related decline in the synaptic response. Thereafter, we further explored whether such functional decline affects the JC firing pattern during the normal feeding response. During the feeding-like rhythmic responses induced by electrical nerve stimulation, the firing of the JC motor neurons advanced toward that of the MA burst, which typically happens during aversive taste responses. These results suggest that the age-related decline in the cholinergic synaptic response may partly cause the JC firing patterns that resemble the aversive taste response in old animals.