RESUMO
A male fetus of gestation day 187 was aborted from a Holstein-Friesian cow in an epizootic of the Aino virus (AINOV) in September 1995. Neutralizing antibody titers against AINOV were 1:128, 1:16 and 1:64 in the dam serum, fetal ascites and cerebrospinal fluid, respectively. A 10% brain suspension of the aborted fetus was prepared immediately after autopsy, rinsed three times and sonicated before centrifugation. The supernatant was then inoculated into HmLu-1 cell cultures. A cytopathic effect was noted on post-inoculation day 7. The isolated virus was identified as the AINOV based on the physicochemical properties and cross neutralization test. This is the first report on the isolation of AINOV from an aborted bovine fetus.
Assuntos
Aborto Animal/virologia , Infecções por Bunyaviridae/veterinária , Doenças dos Bovinos/virologia , Morte Fetal/veterinária , Feto/virologia , Vírus Simbu/isolamento & purificação , Animais , Anticorpos Antivirais/análise , Encéfalo/embriologia , Encéfalo/virologia , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , Bovinos , Doenças dos Bovinos/epidemiologia , Efeito Citopatogênico Viral , Feminino , Morte Fetal/virologia , Masculino , Gravidez , Vigilância de Evento Sentinela/veterinária , Estudos Soroepidemiológicos , Vírus Simbu/classificação , Vírus Simbu/imunologiaRESUMO
A bovine fetus aborted at 187 days of gestation was serologically and immunohistopathologically examined. Serum and cerebrospinal fluid samples had high titers of virus-neutralizing antibody for Aino virus. A severe necrotizing encephalopathy was noted. Aino virus antigen was demonstrated in neuroglial cells within the brain lesion. The destruction of developing neuronal cells appeared to be a significant feature of the pathogenesis of lesions due to Aino virus infection in the central nervous system.