A role for proteoglycans in mineralized tissue-titanium adhesion.

Biomechanical properties of the bone-titanium interface have rarely been studied, due to the technical limitations involved; whether biological bonding mechanisms exist has not been determined. We hypothesized that a selected set of proteoglycan/glycosaminoglycan complexes plays a role in establishing the adhesion between bone and titanium, and utilized the rat bone-marrow-derived osteoblastic culture model to gain an insight into the hypothesis. Gene expression of selected proteoglycan core proteins was up-regulated in the osteoblasts cultured on titanium compared with those on polystyrene. Various sulfated glycosaminoglycans were immunochemically localized at mineralized tissue-titanium interfaces. The administration of various glycosaminoglycan-degrading enzymes into the cultures resulted in a 25-45% reduction of the tissue-titanium interfacial strength, measured by a nanoscratch test; while the hardness and elastic modulus of the mineralized tissue, evaluated by nano-indentation, were not altered. In conclusion, glycosaminoglycan degradation resulted in a decreased interfacial strength between cultured mineralized tissue and titanium, but did not alter the intrinsic strength of the mineralized tissue, suggesting a role for proteoglycan/glycosaminoglycan complexes in the establishment of tissue-titanium adhesion.

Population analyses of kinetic partitioning in protein folding.

A simple lattice model of protein folding is studied in order to analyze the kinetic partitioning phenomena in the energy landscape perspective. By restricting the area of conformational space, it becomes possible to follow many Monte Carlo trajectories until they reach equilibrium. Alteration of population of trajectories is monitored and the relations between the energy landscape and kinetics are examined. Kinetic partitioning phenomena are categorized into different types in terms of characteristic time constants and partitioning ratio. In a specific partitioning process, refolding proceeds along the parallel pathways; the time constants have a temperature dependence similar to that observed in hen lysozyme. High-energy conformations are classified into groups according to the probability that the trajectories starting from those conformations will reach each energy valley. The partitioning ratio is determined by the way in which the conformational space is organized into these groups.

VFEU water quality control in STS-95 mission.

In STS-95 Space Shuttle mission, an aquatic animal research facility, Vestibular Function Experiment Unit (VFEU), was flown to perform neurobiological experiment with marine fish, oyster toadfish. For this purpose, we have developed a sea water purification system using highly active nitrifying bacteria at low temperature. With this system, the water quality in the VFEU was maintained in sufficient condition to keep the toadfish in healthy state for 9 days of the mission. This report summarizes the efficiency of the filter system based on the results from pre-flight bacterial preparation, water analysis of samples taken during flight, and the post-flight analysis of the bacterial filter.

Water quality management for low temperature marine fishes in space.

Vestibular Function Experiment Unit (VFEU), one of the Spacelab facility flown in Neurolab mission (STS-90) in April, 1998, was to support neurophysiological research using a marine fish, Opsanus tau (oyster toadfish). The functions of the VFEU were primarily a quality management of environmental water during the mission at 14 degrees C and for acquiring physiological signals from implanted micro-electrodes in the otolith nerves as well as the spatial acceleration of the fish. A key element of the life support system was a balanced biological filter containing two types of nitrifying bacteria, Nitrosomonas for ammonia oxidization and Nitrobacter for nitrite oxidization. Although the 16 days mission was successful, two toadfishes died in late phase of the mission. Ammonium concentration in those two life support systems elevated to remarkably high level at the end of the mission whereas the other two indicated very low. This report summarizes the results of the water quality management of the VFEU during the Neurolab mission based on analysis of water samples taken during the flight and those taken prior and just after the flight.

R&D of long-term life support system by using electrochemically activated biofilm reactor of aquatic animals for space examinations.

We have developed the long-term life support system that enables the experiment of aquatic animals breeding for 90 days or more for the future experiments in orbit. In order to enable long-term breeding of wide aquatic animals, it is necessary to remove nitrate produced by biological nitrification. Then, we examined a denitrification method to use an electrochemical reaction of biofilm-electrode reactor. In this research, we have not kept the aquatic animals actually but imitated breeding of five goldfish. The ammonia of about 250 ppm was added in breeding water through 90 days. As a result, neither ammonia nor nitrite accumulated 0.1 ppm and nitrate could be suppressed to about 10 ppm.

Water purification system by using the biofilter for long-term experiment equipment with aquatic animals for the space station.

We have developed a water purification system that enables long-term experiment with aquatic animals for 90 days or more on the space station. We designed the system that combined a biofilter for ammonia removal (nitrification) with another for nitrate removal (denitrification). The experiment with goldfish was for 90 days with an aquatic animals' examination device. The equipment consists of a fish tank, a filter module, pumps, and an artificial lung for gas exchange. The goldfish were kept in the tank without any water replacement throughout the experiment. When a filter module consists of adsorbents without bacteria, the concentration of the nitrite and ammonia begin to increase so that the goldfish die. On the contrary, neither ammonia nor nitrite accumulated throughout the experiment, and the concentration of T-N also maintained 30 ppm or less when the combined biofilter was used. Moreover, no fish died throughout the period. The water purification system with biofilter enabled us to examine the long-term life support testing. We also report a new denitrification (correction of dentrification) method for the life support system.