The Relationship among the Performance and the ACE I/D and ACTN3 R577X Polymorphisms and some Anthropometric Factors in Arm Wrestlers.

Sport genetics has become increasingly important in recent years. The concept of performance includes genetic and anthropometric factors. These factors have not been adequately examined when the current literature is examined. One of the aims of this study is to identify a possible interaction between the ACE ID and ACTN3 R577X polymorphisms and the sedentary, national arm wrestlers and amateur arm wrestlers, while another aim is to reveal some physical differences between the same persons. The research included 24 arm wrestlers who were members of the Turkish national team, 23 amateur arm wrestlers with club licenses, and 34 sedentary, all of the wrestlers participated voluntarily in the research. The genotype distribution of the ACE gene and ACTN gene and the statistical significance of the R, X, I, and D allele frequencies were compared by the Chi-Square test. The Anova one-way variance analysis was used to assess the difference among the palm circumference, wrist circumference, and forearm width among the groups, the significance was tested at p<.05 level. After the data was evaluated,  significant differences were not found statistically in ACE nor ACTN3 polymorphisms in terms of the three groups (p>0.05). In addition, statistically significant differences were found in the palm, wrist, and forearm circumferences of the arm wrestlers who were the members of the Turkish national team compared especially to the sedentary (p<0.05). From the conducted research it was concluded that the success of the athlete in arm wrestling could not be directly explained by the ACE and ACTN3 genotypic characteristics. Another result revealed by this study was that the success of the athlete in arm wrestling was more related to palm, wrist, and forearm circumferences.

Effects of Pyrroloquinoline Quinone (PQQ) and Coenzyme Q10 on Mitochondrial Genes, MitomiRs and Cellular Properties in HepG2 Cell Line.

Our study aimed to reveal the effects and changes, antioxidant metabolism (Oxidative Stress), inflammatory response, mitochondrial biogenesis and mitochondrial dysfunction characteristics in hepatocellular carcinoma cell line HepG2; that occur in genes (NRF-1, NRF-2, NFκB and PGC-1α) and miRNAs (miR15-a, miR16-1, miR181-c) that can control related features. To investigate the effects of Pyrroloquinoline quinone (PQQ) and Coenzyme Q10 (CoQ10) in HepG2, and their effects on cell viability, lateral cell migration, gene expression and miRNA expression levels were investigated. If the data we have obtained are evaluated in terms of anti-cancer effectiveness, the most effective use of CoQ10 can be defined as the use alone rather than the combined use. According to the results of the wound healing experiment, we determined that Pyrroloquinoline quinone and combined drug application increased the wound closure area and cell proliferation compared to the control group, while CoQ10 application decreased it. We found that Pyrroloquinoline quinone and Coenzyme Q10 exposure in the HepG2 cell line increased Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) expression but not NRF-1 gene expression. We reported only a small increase in expression of the NRF-2 gene in the Pyrroloquinoline quinone application compared to the control group. We found that only Pyrroloquinoline quinone and CoQ10 application caused more expression increase in the Nuclear Factor kappa B (NFκB) gene compared to combined application. Pyrroloquinoline quinone and CoQ10 administration down-regulated the expression levels of miR16-1, miR15a and miR181c. The use of Pyrroloquinoline quinone and CoQ10 is effective on epigenetic factors, miR-15a, miR-16-1 and miR181c are important candidate biomarkers in hepatocellular carcinoma and diseases accompanied by mitochondrial dysfunction.