A topographic study of minicolumnar core width by lamina comparison between autistic subjects and controls: possible minicolumnar disruption due to an anatomical element in-common to multiple laminae.

Radial cell minicolumns are basic cytoarchitectonic motifs of the mammalian neocortex. Recent studies reveal that autism is associated with a "minicolumnopathy" defined by decreased columnar width and both a diminished and disrupted peripheral neuropil compartment. This study further characterizes this cortical deficit by comparing minicolumnar widths across layers. Brains from seven autistic patients and an equal number of age-matched controls were celloidin embedded, serially sectioned at 200 microm and Nissl stained with gallocyanin. Photomicrograph mosaics of the cortex were analyzed with computerized imaging methods to determine minicolumnar width at nine separate neocortical areas: Brodmann Area's (BA) 3b, 4, 9, 10, 11, 17, 24, 43 and 44. Each area was assessed at supragranular, granular and infragranular levels. Autistic subjects had smaller minicolumns whose dimensions varied according to neocortical area. The greatest difference between autistic and control groups was observed in area 44. The interaction of diagnosis x cortical area x lamina (F(16,316) = 1.33; P = 0.175) was not significant. Diminished minicolumnar width across deep and superficial neocortical layers most probably reflects involvement of shared constituents among the different layers. In this article we discuss the possible role of double bouquet and pyramidal cells in the translaminar minicolumnar width narrowing observed in autistic subjects.

Minicolumnar width: Comparison between supragranular and infragranular layers.

The minicolumn derives from the radial migration of neurons along glial scaffoldings during gestation. Investigators have presumed the minicolumn to be a single-cell wide structure based on their rectilinear migratory origin. The present study measures the width of minicolumnar cores in both supra- and infra-granular layers. Postmortem tissue was obtained from 9 brain areas in 7 normative individuals. Examined tissues were celloidin embedded and Nissl stained. Digital images were denoised and then analyzed with a step-wise algorithm involving region growing and recursive line tracing. Significant differences were noted between the minicolumnar core widths of supra- and infra-granular layers. A review of the literature on corticogenesis provides some ideas as to how these laminar differences in minicolumnar core width are engendered.