TLC-ESI-MS as a QuEChERS approach to detection of capsaicin present in different matrices.

Pepper is one of the most consumed spices in the world. Its main compound is capsaicin, a widely studied biomarker that has pharmacological activities due to its anti-inflammatory and analgesic capacity. Topical formulations such as patches based on capsaicin have been developed as an option in relieving pain and reducing swelling. In addition, capsaicin is used as an active ingredient in non-lethal weapon formulations such as pepper spray through the QuEChERS concept (Quick, Easy, Cheap, Effective, Robust, Safe) technique. Used for food analysis, it allows the direct analysis of the biomarker by TLC-ESI-MS, which are previously separated by HPTLC (High Performance Thin Layer Chromatography) using an internal standard for determination of Rf and confirmation of capsaicin in different matrices.[Formula: see text].

A Bibliometric Network Analysis of Recent Publications on Digital Agriculture to Depict Strategic Themes and Evolution Structure.

The agriculture sector is one of the backbones of many countries' economies. Its processes have been changing to enable technology adoption to increase productivity, quality, and sustainable development. In this research, we present a scientific mapping of the adoption of precision techniques and breakthrough technologies in agriculture, so-called Digital Agriculture. To do this, we used 4694 documents from the Web of Science database to perform a Bibliometric Performance and Network Analysis of the literature using SciMAT software with the support of the PICOC protocol. Our findings presented 22 strategic themes related to Digital Agriculture, such as Internet of Things (IoT), Unmanned Aerial Vehicles (UAV) and Climate-smart Agriculture (CSA), among others. The thematic network structure of the nine most important clusters (motor themes) was presented and an in-depth discussion was performed. The thematic evolution map provides a broad perspective of how the field has evolved over time from 1994 to 2020. In addition, our results discuss the main challenges and opportunities for research and practice in the field of study. Our findings provide a comprehensive overview of the main themes related to Digital Agriculture. These results show the main subjects analyzed on this topic and provide a basis for insights for future research.

Neuroprotective and anti-inflammatory effect of pectolinarigenin, a flavonoid from Amazonian Aegiphila integrifolia (Jacq.), against lipopolysaccharide-induced inflammation in astrocytes via NFκB and MAPK pathways.

Neurodegenerative diseases affect millions of people worldwide. Regardless of the underlying cause, neuroinflammation is the greatest risk factor for developing any of these disorders. Pectolinarigenin (PNG) is an active flavonoid with several biological properties, anti-metastatic and anti-inflammatory activity. This study investigate the biological effects of PNG in macrophage and astrocyte cultures, with focus on elucidating the molecular mechanisms involved in the PNG activity. J774A.1 murine macrophage or cerebral cortex primary astrocytes primary cultures were treated with different concentration of PNG (1-160 µM) and the inflammatory process was stimulated by LPS (1 µg/ml) and the effect of PNG in different inflammatory markers were determined. PNG did not affect astrocyte or macrophage viability. Moreover, this flavonoid reduced NO• release in macrophages, attenuated astrocyte activation by preventing the overexpression of glial fibrillary acidic protein, and decreased the release of inflammatory mediators, IL-1ß and IL-6 induced by LPS by the glial cell, as well as enhanced basal levels of IL-10. In addition, PNG suppressed NFκB, p38MAPK and ERK1/2 phosphorylation in astrocytes culture induced by LPS. The results show clear evidence that this novel flavonoid protects astrocytes against LPS-induced inflammatory toxicity. In conclusion, PNG presents neuroprotective and anti-inflammatory property through the inhibition of inflammatory signaling pathways.