Antimicrobial activity of nanocellulose composite hydrogel isolated from an agricultural waste.

Infectious diseases and antimicrobial resistance have become one of the extreme health threats of this century. Overuse of antibiotics leads to pollution. To overcome this threat, the current strategy is to develop a substitute for these antibiotics that are extracted from natural sources. In this study, nanocellulose (NC) was isolated from an agricultural waste (wheat straw) and then oxidized with the help of sodium periodate to obtain dialdehyde nanocellulose (DA-NC). Then, chitosan (Ch) and DA-NC are both crosslinked with each other in different weight ratios, to obtain NC/Ch composite hydrogels. The resulted hydrogel is also characterized to confirm its structure, morphology and composition. The hydrogel was also tested for antimicrobial activities against bacteria, algae as well as fungal species to check its applicability for biomedical applications. The six microbes used for the ananlysis are Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Candida albicans, Aspergillus niger and Fusarium solani. The antimicrobial assessment of the hydrogel is evaluated via inhibition zone and optical density analysis. The resulted nanocellulose/chitosan (NC/Ch) hydrogel shows the uniform distribution of nanocellulose in the composite and the synergistic effect of their properties. Hydrogel serves excellent antimicrobial results which makes it a promising candidate for various biomedical applications.

Eco-friendly nanocellulose and its biomedical applications: current status and future prospect.

Cellulose is the earth's leading natural polymer. It is known for its properties like biocompatibility, high mechanical strength, cost-effectiveness and lightweight. Nanocellulose displays better properties as compared to the native cellulose fibre. The nanocellulose is very remunerative in the arenas of routine application especially in health care, food industry, sanitary products and many more. In the biomedical area, cellulose-based products are utilized in applications like wound healing, dental applications, drug delivery, antimicrobial material, etc. Nanocellulose biomaterials have been commercialised, representing the material of new generation. With the objective to comprehend the contribution of nanocellulose in the current status and future development in biomedical utilisations, the review is focused on cellulose, nanocellulose, types and sources of nanocellulose, its preparation, characteristics, constraints related to its composites through the analysis of certain scientific reports.

Synthesis, characterization and investigation of synergistic antibacterial activity and cell viability of silver-sulfur doped graphene quantum dot (Ag@S-GQDs) nanocomposites.

The excessive use of traditional antibiotic and antibacterial agents has globally increased the growth of antibiotic-resistant bacteria that poses serious health risks. Therefore, the development of new generation antibacterial or antimicrobial agents for effective inhibition of bacterial growth is highly desired. In this study, we report a facile one-step synthesis approach for the preparation of a nanocomposite composed of silver nanoparticles (AgNPs) decorated with sulfur-doped graphene quantum dots (S-GQDs). The nanocomposite was comprehensively characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), UV-vis absorption spectra, Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The characterization results demonstrated that the AgNPs were closely and uniformly surrounded by the S-GQDs, and consequently, this ensured the dispersion and stability of the so formed nanocomposite (Ag@S-GQDs). Further, the antibacterial activity of the Ag@S-GQDs nanocomposite was investigated and compared with bare S-GQDs and AgNPs against Gram-positive S. aureus (MTCC 737) and Gram-negative P. aeruginosa (MTCC 424) bacteria using macrodilution and agar well diffusion methods. Minimum inhibitory concentration (MIC) values of 70 and 35 µg mL-1 of the Ag@S-GQDs nanocomposite were found to be sufficient to hinder the growth of P. aeruginosa and S. aureus. A fractional inhibition concentration (FIC) index below 0.5 confirmed the existence of a synergistic effect between AgNPs and S-GQDs in the Ag@S-GQDs nanocomposite. In addition, the cytotoxicity of the Ag@S-GQDs nanocomposite, AgNPs and S-GQDs was also investigated using HEK 293 cell lines. Interestingly, the Ag@S-GQDs nanocomposite exhibited superior cell viability as compared to AgNPs and S-GQDs. These improved antibacterial and biocompatibility data demonstrate that the Ag@S-GQDs nanocomposite can serve as a promising antibacterial agent for industry to fabricate next-generation self-sterile textiles, antibacterial coatings and useful health care products supporting cell viability.

Effect of Addition of Fentanyl and Clonidine to Local Anesthetic Solution in Peribulbar Block.

OBJECTIVE: To compare the effect of addition of fentanyl and clonidine as adjuvants to bupivacaine and lignocaine in peribulbar block. METHODS: The study was conducted on 105 adult patients of either sex, of ASA grade I and II undergoing ophthalmic surgeries. Patients were randomly divided into 3 groups of 35 each. All the patients were given peribulbuar block with 5ml lignocaine 2% +3 ml bupivacaine 0.5% +1 ml hyaluronidase (250 IU). In addition to this 1 ml normal saline was added to Group S, 25 µg fentanyl to Group F and 25 µg clonidine to Group C. Onset and duration of globe and lid akinesia, duration of sensory blockage and analgesia, hemodynamic parameters, number of rescue analgesic and visual analogue score were recorded. RESULTS: The mean time of onset of globe and lid akinesia was significantly faster in group F and group C compared to group S, mean duration of globe and lid akinesia was longer in Group F (207.71 + 13.54 and 143.14 + 7.86 min) and group C (213.52 + 14.52 and 162.06 + 17.1 min) compared to group S (117.78 + 10.42 and 87.64 + 9.76 min). The mean duration of analgesia was significantly longer in group F (217.71 + 12.67) and C (258.82 + 14.50 min) as compared to group S (131.39 + 9.63 min). CONCLUSION: Addition of fentanyl or clonidine as adjuvant to local anaesthetic in peribulbar block provides faster onset and prolonged analgesia compared to local anaesthetic alone.

Comparison of fractionated dose versus bolus dose injection in spinal anaesthesia for patients undergoing elective caesarean section: A randomised, double-blind study.

BACKGROUND AND AIMS: Spinal anaesthesia (SA) with bolus dose has rapid onset but may precipitate hypotension. When we inject local anaesthetic in fractions with a time gap, it provides a dense block with haemodynamic stability and also prolongs the duration of analgesia. We aimed to compare fractionated dose with bolus dose in SA for haemodynamic stability and duration of analgesia in patients undergoing elective lower segment caesarean section (LSCS). METHODS: After clearance from the Institutional Ethics Committee, the study was carried out in sixty patients undergoing elective LSCS. Patients were divided into two groups. Group B patients received single bolus SA with injection bupivacaine heavy (0.5%) and Group F patients fractionated dose with two-third of the total dose of injection bupivacaine heavy (0.5%) given initially followed by one-third dose after 90 s. Time of onset and regression of sensory and motor blockage, intraoperative haemodynamics and duration of analgesia were recorded and analysed with Student's unpaired t-test. RESULT: All the patients were haemodynamically stable in Group F as compared to Group B. Five patients in Group F and fourteen patients in Group B required vasopressor. Duration of sensory and motor block and duration of analgesia were longer in Group F (273.83 ± 20.62 min) compared to Group B (231.5 ± 31.87 min) P < 0.05. CONCLUSION: Fractionated dose of SA provides greater haemodynamic stability and longer duration of analgesia compared to bolus dose.