Protein binding and antioxidant studies of diimine based emissive surfactant-ruthenium(II) complexes.

Biophysical interaction of amphiphilic fluorescent surfactant-ruthenium(II) complexes and its precursor ruthenium(II) complexes with drug carrying proteins such as bovine and human serum albumins (BSA and HSA) have been studied through the UV-visible absorption, fluorescence and circular dichroism spectroscopic techniques to correlate the impact of head and tail groups of the metallosurfactants towards the designing of metallodrugs for the biomedical applications. The obtained results showed that both precursor- and surfactant-ruthenium(II) complexes interact with BSA/HSA via ground state protein-complex formation and their quenching follows the static mechanism. The extent of protein quenching and binding parameters resulted that the surfactant-ruthenium(II) complexes effectively interact with protein compared to their precursor-ruthenium(II) complexes, and also those interaction have greatly influenced by the change in the head group size compared to change in the tail group length. Interestingly on increasing the temperature, the protein-complex binding strength was decreased for the precursor-ruthenium(II) complexes, those increased for the surfactant-ruthenium(II) complexes, probably due to the respective involvement of electrostatic and hydrophobic interactions as supported by the thermodynamics of protein-complex interaction. Moreover, the results from UV-visible, synchronous and circular dichroism studies confirmed the occurrence of conformational and micro environmental changes in BSA/HSA upon binding with these complexes. It is also noted that HSA has more binding affinity with surfactant-ruthenium(II) complexes compared to BSA. The free radical scavenging ability against DPPH, ABTS, NO and superoxide free radical assays suggested that surfactant-ruthenium(II) complexes have better free radical scavenging ability compared to precursor-ruthenium(II) complexes.Communicated by Ramaswamy H. Sarma.

Reduced Graphene Oxide/Multiwalled Carbon Nanotube Composite Decorated with Fe3O4 Magnetic Nanoparticles for Electrochemical Determination of Hydrazine in Environmental Water.

In the present work, a reduced graphene oxide and multiwalled carbon nanotube (RGO/MWCNTFe3O4) composite decorated with Fe3O4 magnetic nanoparticles was prepared as an electrochemical sensor. The surface morphology of the prepared composite was identified by scanning electron microscopy and X-ray diffraction. The electrochemical properties of the GCE/RGO/MWCNT-Fe3O4 electrode were investigated by electrochemical impedance spectroscopy, cyclic voltammetry and amperometry. The GCE/RGO/MWCNT-Fe3O4 electrode exhibited higher electrocatalytic performance towards the oxidation of hydrazine. In the optimal conditions, the GCE/RGO/MWCNT-Fe3O4 electrode showed a wide linear range (0.15-220 µM), low limit of detection (LOD) (0.75 µM), and high sensitivity (2.868 µA µM-1 cm-2). The prepared GCE/RGO/MWCNT-Fe3O4 electrode also had excellent repeatability, selectivity, and reproducibility. The practical application of the electrode was confirmed with various spiked water samples and demonstrated acceptable recovery.

Phytoconstituents and their Possible Mechanistic Profile for Alzheimer's Disease - A Literature Review.

Memory is an associated part of life without which livelihood of a human being becomes miserable. As the global aged population is increasing tremendously, time has come to concentrate on tail end life stage diseases. Alzheimer's disease (AD) is one of such diseases whose origin is enigmatic, having an impact on later stage of life drastically due to irreparable damage of cognition, characterised by the presence of neurotoxic amyloid-beta (Aß) plaques and hyper phosphorylated Tau protein as fibrillary tangles. Existing therapeutic regimen mainly focuses on symptomatic relief by targeting neurotransmitters that are secondary to AD pathology. Plant derived licensed drugs, Galantamine and Huperzine-A were studied extensively due to their AChE inhibitory action for mild to moderate cases of AD. Although many studies have proved the efficacy of AChEIs as a preferable symptom reliever, they cannot offer long term protection. The future generation drugs of AD is expected to alter various factors that underlie the disease course with a symptomatic benefit promise. As AD involves complex pathology, it is essential to consider several molecular divergent factors apart from the events that result in the production of toxic plaques and neurofibrillary tangles. Even though several herbals have shown neuroprotective actions, we have mentioned about the phytoconstituents that have been tested experimentally against different Alzheimer's pathology models. These phytoconstituents need to be considered by the researchers for further drug development process to make them viable clinically, which is currently a lacuna.

Exposing water samples to ultraviolet light improves fluorometry for detecting human fecal contamination.

Fluorometry identifies human fecal contamination by detecting optical brighteners in environmental waters. Because optical brighteners are sensitive to sunlight, we determined if we could improve fluorometry by exposing water samples to ultraviolet (UV) light to differentiate between optical brighteners and other fluorescing organic compounds. Optical brighteners were likely present when the relative percentage difference in fluorometric value of the water before and after UV light exposure was >30% (glass cuvettes, 30 min exposure) or >15% (polymethacrylate cuvettes, 5 min exposure). In a blind study, we correctly identified the presence or absence of optical brighteners in 178 of 180 (99%) of the samples tested with a more expensive field fluorometer and in 175 of 180 (97%) of the samples tested with a less expensive handheld fluorometer. In the field, the method correctly identified two negative and three positive locations for human fecal contamination. When combined with counts of fecal bacteria, the new fluorometric method may be a simple, quick, and easy way to identify human fecal contamination in environmental waters.