Inside and outside of virus-like particles HBc and HBc/4M2e: A comprehensive study of the structure.

Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (HBc/4M2e) form virus-like particles whose structure was studied using a combination of molecular modeling and cryo-electron microscopy (cryo-EM). It was also shown that self-assembling of the particles occurs inside bacterial cells, but despite the big inner volume of the core shell particle, purified HBc/4M2e contain an insignificant amount of bacterial proteins. It was shown that a fragment of the M2e corresponding to 4M2e insertion is prone to formation of amyloid-like fibrils. However, as the part of the immunodominant loop, M2e insertion does not show a tendency to intermolecular interaction. A full-atomic HBc-4M2e model with the resolution of about 3 Å (3.13 Å for particles of Т = 4 symmetry, 3.7 Å for particles of Т = 3 symmetry) was obtained by molecular modeling methods based on cryo-EM data.

Study of protein components of natural peptide regulators.

Special attention should be given to the safety of drugs prepared from animal products. Industrial production of cytomedines and cytamines includes treatment of organs and tissues from young animals. Studies of preparations by methods recommended by the World Health Organization indicate that this technology guarantees the absence of infectious agents, protooncogenes, nucleic acids, and prion proteins.

[Role of feedback in the mechanism of the initial phase of gastric secretion]. / O roli obratnykh sviazei v mekhanizme pervoi fazy zheludochnoi sekretsii.

In chronic experiments on dogs with gastric and esophageal fistulas, the feed-back from the gastro-intestinal tract was found not to play the leading role in the maintenance of continuous nervous excitation underlying the first phase of the gastric secretion.