Long-term trend and interannual variation in evapotranspiration of a young temperate Douglas-fir stand over 2002-2022 reveals the impacts of climate change.

The shortage of decades-long continuous measurements of ecosystem processes limits our understanding of how changing climate impacts forest ecosystems. We used continuous eddy-covariance and hydrometeorological data over 2002-2022 from a young Douglas-fir stand on Vancouver Island, Canada to assess the long-term trend and interannual variability in evapotranspiration (ET) and transpiration (T). Collectively, annual T displayed a decreasing trend over the 21 years with a rate of 1% yr-1, which is attributed to the stomatal downregulation induced by rising atmospheric CO2 concentration. Similarly, annual ET also showed a decreasing trend since evaporation stayed relatively constant. Variability in detrended annual ET was mostly controlled by the average soil water storage during the growing season (May-October). Though the duration and intensity of the drought did not increase, the drought-induced decreases in T and ET showed an increasing trend. This pattern may reflect the changes in forest structure, related to the decline in the deciduous understory cover during the stand development. These results suggest that the water-saving effect of stomatal regulation and water-related factors mostly determined the trend and variability in ET, respectively. This may also imply an increase in the limitation of water availability on ET in young forests, associated with the structural and compositional changes related to forest growth.

Arctic soil methane sink increases with drier conditions and higher ecosystem respiration.

Arctic wetlands are known methane (CH4) emitters but recent studies suggest that the Arctic CH4 sink strength may be underestimated. Here we explore the capacity of well-drained Arctic soils to consume atmospheric CH4 using >40,000 hourly flux observations and spatially distributed flux measurements from 4 sites and 14 surface types. While consumption of atmospheric CH4 occurred at all sites at rates of 0.092 ± 0.011 mgCH4 m-2 h-1 (mean ± s.e.), CH4 uptake displayed distinct diel and seasonal patterns reflecting ecosystem respiration. Combining in situ flux data with laboratory investigations and a machine learning approach, we find biotic drivers to be highly important. Soil moisture outweighed temperature as an abiotic control and higher CH4 uptake was linked to increased availability of labile carbon. Our findings imply that soil drying and enhanced nutrient supply will promote CH4 uptake by Arctic soils, providing a negative feedback to global climate change.

Re-assessment of the climatic controls on the carbon and water fluxes of a boreal aspen forest over 1996-2016: Changing sensitivity to long-term climatic conditions.

Recent evidence suggests that the relationships between climate and boreal tree growth are generally non-stationary; however, it remains uncertain whether the relationships between climate and carbon (C) fluxes of boreal forests are stationary or have changed over recent decades. In this study, we used continuous eddy-covariance and microclimate data over 21 years (1996-2016) from a 100-year-old trembling aspen stand in central Saskatchewan, Canada to assess the relationships between climate and ecosystem C and water fluxes. Over the study period, the most striking climatic event was a severe, 3-year drought (2001-2003). Gross ecosystem production (GEP) showed larger interannual variability than ecosystem respiration (Re ) over 1996-2016, but Re was the dominant component contributing to the interannual variation in net ecosystem production (NEP) during post-drought years. The interannual variations in evapotranspiration (ET) and C fluxes were primarily driven by temperature and secondarily by water availability. Two-factor linear models combining precipitation and temperature performed well in explaining the interannual variation in C and water fluxes (R2 > .5). The temperature sensitivities of all three C fluxes (NEP, GEP and Re ) declined over the study period (p < .05), and, as a result, the phenological controls on annual NEP weakened. The decreasing temperature sensitivity of the C fluxes may reflect changes in forest structure, related to the over-maturity of the aspen stand at 100 years of age, and exacerbated by high tree mortality following the severe 2001-2003 drought. These results may provide an early warning signal of driver shift or even an abrupt status shift of aspen forest dynamics. They may also imply a universal weakening in the relationship between temperature and GEP as forests become over-mature, associated with the structural and compositional changes that accompany forest ageing.

Seasonal variation in the canopy color of temperate evergreen conifer forests.

Evergreen conifer forests are the most prevalent land cover type in North America. Seasonal changes in the color of evergreen forest canopies have been documented with near-surface remote sensing, but the physiological mechanisms underlying these changes, and the implications for photosynthetic uptake, have not been fully elucidated. Here, we integrate on-the-ground phenological observations, leaf-level physiological measurements, near surface hyperspectral remote sensing and digital camera imagery, tower-based CO2 flux measurements, and a predictive model to simulate seasonal canopy color dynamics. We show that seasonal changes in canopy color occur independently of new leaf production, but track changes in chlorophyll fluorescence, the photochemical reflectance index, and leaf pigmentation. We demonstrate that at winter-dormant sites, seasonal changes in canopy color can be used to predict the onset of canopy-level photosynthesis in spring, and its cessation in autumn. Finally, we parameterize a simple temperature-based model to predict the seasonal cycle of canopy greenness, and we show that the model successfully simulates interannual variation in the timing of changes in canopy color. These results provide mechanistic insight into the factors driving seasonal changes in evergreen canopy color and provide opportunities to monitor and model seasonal variation in photosynthetic activity using color-based vegetation indices.

Divergent long-term trends and interannual variation in ecosystem resource use efficiencies of a southern boreal old black spruce forest 1999-2017.

Long-term trends in ecosystem resource use efficiencies (RUEs) and their controlling factors are key pieces of information for understanding how an ecosystem responds to climate change. We used continuous eddy covariance and microclimate data over the period 1999-2017 from a 120-year-old black spruce stand in central Saskatchewan, Canada, to assess interannual variability, long-term trends, and key controlling factors of gross ecosystem production (GEP) and the RUEs of carbon (CUE = net primary production [NPP]/GEP), light (LUE = GEP/absorbed photosynthetic radiation [APAR]), and water (WUE = GEP/evapotranspiration [E]). At this site, annual GEP has shown an increasing trend over the 19 years (p < 0.01), which may be attributed to rising atmospheric CO2 concentration. Interannual variability in GEP, aside from its increasing trend, was most strongly related to spring temperatures. Associated with the significant increase in annual GEP were relatively small changes in NPP, APAR, and E, so that annual CUE showed a decreasing trend and annual LUE and WUE showed increasing trends over the 19 years. The long-term trends in the RUEs were related to the increasing CO2 concentration. Further analysis of detrended RUEs showed that their interannual variation was impacted most strongly by air temperature. Two-factor linear models combining CO2 concentration and air temperature performed well (R2 ~0.60) in simulating annual RUEs. LUE and WUE were positively correlated both annually and seasonally, while LUE and CUE were mostly negatively correlated. Our results showed divergent long-term trends among CUE, LUE, and WUE and highlighted the need to account for the combined effects of climatic controls and the 'CO2 fertilization effect' on long-term variations in RUEs. Since most RUE-based models rely primarily on one resource limitation, the observed patterns of relative change among the three RUEs may have important implications for RUE-based modeling of C fluxes.

Greater Impacts of Incubation Temperature and Moisture on Carbon and Nitrogen Cycling in Poultry Relative to Horse Manure-based Soil Amendments.

Manure-based soil amendments (MBSAs) must be managed optimally to maximize N concentration and availability while minimizing environmental impacts (e.g., greenhouse gas [GHG]) emissions. We conducted an 83-d incubation study to determine the effects of different moisture (60 or 120% of water-holding capacity [WHC]) and temperature (4 or 20°C) conditions during the decomposition of MBSAs. We measured CO, CH, and NO emissions and total C, total N, NH, and NO during the decomposition of chicken MBSA and two understudied MBSAs (turkey and horse). Total N decreased by 38 to 50% after 83 d in poultry MBSAs incubated at 20°C and 120% WHC, whereas NH concentration peaked at 30 d. In contrast, poultry MBSAs incubated at 60% WHC or 4°C had limited N losses but higher CO and/or NO emissions. Horse MBSA incubated for 83 d at 20°C and 60% WHC had two- to threefold higher C losses, 53 to 68% higher total N, and two to three orders of magnitude higher NO concentrations than at wetter and/or colder incubation conditions. Horse MBSA incubated at 20°C and 60% WHC had 13- to 130-fold (CH) and 4- to 70-fold (NO) higher emissions than horse MBSA incubated at 4°C. In contrast, CH emissions peaked at 120% WHC and 20°C. Overall, incubating horse MBSA at 20°C and 60% WHC minimized tradeoffs between maximizing N concentration and availability and minimizing GHG emissions during decomposition, whereas we found no ideal decomposition conditions for poultry MBSAs.

Protection from wintertime rainfall reduces nutrient losses and greenhouse gas emissions during the decomposition of poultry and horse manure-based amendments.

Manure-based soil amendments (herein "amendments") are important fertility sources, but differences among amendment types and management can significantly affect their nutrient value and environmental impacts. A 6-month in situ decomposition experiment was conducted to determine how protection from wintertime rainfall affected nutrient losses and greenhouse gas (GHG) emissions in poultry (broiler chicken and turkey) and horse amendments. Changes in total nutrient concentration were measured every 3 months, changes in ammonium (NH4+) and nitrate (NO3-) concentrations every month, and GHG emissions of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) every 7-14 days. Poultry amendments maintained higher nutrient concentrations (except for K), higher emissions of CO2 and N2O, and lower CH4 emissions than horse amendments. Exposing amendments to rainfall increased total N and NH4+ losses in poultry amendments, P losses in turkey and horse amendments, and K losses and cumulative N2O emissions for all amendments. However, it did not affect CO2 or CH4 emissions. Overall, rainfall exposure would decrease total N inputs by 37% (horse), 59% (broiler chicken), or 74% (turkey) for a given application rate (wet weight basis) after 6 months of decomposition, with similar losses for NH4+ (69-96%), P (41-73%), and K (91-97%). This study confirms the benefits of facilities protected from rainfall to reduce nutrient losses and GHG emissions during amendment decomposition. IMPLICATIONS: The impact of rainfall protection on nutrient losses and GHG emissions was monitored during the decomposition of broiler chicken, turkey, and horse manure-based soil amendments. Amendments exposed to rainfall had large ammonium and potassium losses, resulting in a 37-74% decrease in N inputs when compared with amendments protected from rainfall. Nitrous oxide emissions were also higher with rainfall exposure, although it had no effect on carbon dioxide and methane emissions. Overall, this work highlights the benefits of rainfall protection during amendment decomposition to reduce nutrient losses and GHG emissions.

Technological Advancement in Tower-Based Canopy Reflectance Monitoring: The AMSPEC-III System.

Understanding plant photosynthesis, or Gross Primary Production (GPP), is a crucial aspect of quantifying the terrestrial carbon cycle. Remote sensing approaches, in particular multi-angular spectroscopy, have proven successful for studying relationships between canopy-reflectance and plant-physiology processes, thus providing a mechanism to scale up. However, many different instrumentation designs exist and few cross-comparisons have been undertaken. This paper discusses the design evolution of the Automated Multiangular SPectro-radiometer for Estimation of Canopy reflectance (AMSPEC) series of instruments. Specifically, we assess the performance of the PP-Systems Unispec-DC and Ocean Optics JAZ-COMBO spectro-radiometers installed on an updated, tower-based AMSPEC-III system. We demonstrate the interoperability of these spectro-radiometers, and the results obtained suggest that JAZ-COMBO can successfully be used to substitute more expensive measurement units for detecting and investigating photosynthesis and canopy spectra. We demonstrate close correlations between JAZ-COMBO and Unispec-DC measured canopy radiance (0.75 ≤ R² ≤ 0.85) and solar irradiance (0.95 ≤ R² ≤ 0.96) over a three month time span. We also demonstrate close agreement between the bi-directional distribution functions obtained from each instrument. We conclude that cost effective alternatives may allow a network of AMSPEC-III systems to simultaneously monitor various vegetation types in different ecosystems. This will allow to scale and improve our understanding of the interactions between vegetation physiology and spectral characteristics, calibrate broad-scale observations to stand-level measurements, and ultimately lead to improved understanding of changing vegetation spectral features from satellite.

Portable chamber system for measuring chloroform fluxes from terrestrial environments--methodological challenges.

This study describes a system designed to measure chloroform flux from terrestrial systems, providing a reliable first assessment of the spatial variability of flux over an area. The study takes into account that the variability of ambient air concentrations is unknown. It includes quality assurance procedures, sensitivity assessments, and testing of materials used to ensure that the flux equation used to extrapolate from concentrations to fluxes is sound and that the system does not act as a sink or a source of chloroform. The results show that many materials and components commonly used in sampling systems designed for CO2, CH4, and N2O emit chloroform and other volatile chlorinated compounds (VOCls) and are thus unsuitable in systems designed for studies of such compounds. To handle the above-mentioned challenges, we designed a system with a non-steady-state chamber and a closed-loop air-circulation unit returning scrubbed air to the chamber. Based on empirical observations, the concentration increase during a deployment was assumed to be linear. Four samples were collected consecutively and a line was fitted to the measured concentrations. The slope of the fitted line and the y-axis intercept were input variables in the equation used to transform concentration change data to flux estimates. The soundness of the flux equation and the underlying assumptions were tested and found to be reliable by comparing modeled and measured concentrations. Fluxes of chloroform in a forest clear-cut on the east coast of Vancouver Island, BC, during the year were found to vary from -130 to 620 ng m(-2) h(-1). The study shows that the method can reliably detect differences of approximately 50 ng m(-2) h(-1) in chloroform fluxes. The statistical power of the method is still comparatively strong down to differences of 35 ng m(-2) h(-1), but for smaller differences, the results should be interpreted with caution.

Incorporating diffuse photosynthetically active radiation in a single-leaf model of canopy photosynthesis for a 56-year-old Douglas-fir forest.

A simple top-down model of canopy photosynthesis (P) was developed and tested in this study. The model (referred to as the Q(e)-MM model) is P = alphaQ (e) P (max)/(alphaQ ( e ) + P (max)), alpha and P (max) are quantum-use efficiency and potential P, respectively. Q (e) is given by Q (d) (0) + kQ (b) (0), where Q (d) (0) and Q (b) (0) are the diffuse and direct photosynthetically active radiation (PAR) incident on the canopy, respectively. Q (e) can be considered to be the effective incident PAR contributing to P and k is a measure of the contribution of Q (b) (0) to Q (e). When k = 1, the Q(e)-MM model becomes the regular Michaelis-Menten type model of P (referred to as the MM model). A major objective of this study was to determine how well the Q(e)-MM model could estimate P of a 56-year-old coastal Douglas-fir stand. To this end, we parameterized the Q(e)-MM model using five and half years of eddy-covariance measurements of CO(2) flux above the Douglas-fir stand. The Q(e)-MM model, with the incorporation of a function of air temperature, accounted for 74% of the variance in over 34,000 half-hourly P measurements. P estimated using the Q(e)-MM model had no systematic errors with respect to Q (d) (0). Although the Q(e)-MM model has only one more parameter than the MM model, it accounted for 30% more variance in P than the latter when total incident PAR exceeded 900 micromol m(-2) s(-1). On average, k was found to be 0.22. We show that this small value of k reflects the significant effect of the scattering of the solar beam and the fraction of light-limited sunlit leaves. We also show that the success of the Q(e)-MM model was due to the fact that a large fraction of the sunlit leaves were light-limited as a result of their orientation to the solar beam.

Biophysical controls on rhizospheric and heterotrophic components of soil respiration in a boreal black spruce stand.

We conducted a root-exclusion experiment in a 125-year-old boreal black spruce (Picea mariana (Mill.) BSP) stand in 2004 to quantify the physical and biological controls on temporal dynamics of the rhizospheric (R(r)) and heterotrophic (R(h)) components of soil respiration (R(s)). Annual R(r), R(h) and estimated moss respiration were 285, 269 and 57 g C m(-2) year(-1), respectively, which accounted for 47, 44 and 9% of R(s) (611 g C m(-2) year(-1)), respectively. A gradual transition from R(h)-dominated (winter, spring and fall) to R(r)-dominated (summer) respiration was observed during the year. Soil thawing in spring and the subsequent increase in soil water content (theta) induced a small and sustained increase in R(h) but had no effect on R(r). During the remainder of the growing season, no effect of theta was observed on either component of R(s). Both components increased exponentially with soil temperature (T(s)) during the growing season, but R(r) showed greater temperature sensitivity than R(h) (Q(10) of 4.0 and 3.0, respectively). Temperature-normalized variations in R(r) were highly correlated with eddy covariance estimates of gross ecosystem photosynthesis, and the correlation was greatest when R(r) was lagged by 24 days. Within diurnal cycles, variations in T(s) were highly coupled to variations in R(h) but were significantly decoupled from R(r). The patterns observed at both time scales strongly suggest that the flow of photosynthates to the rhizosphere is a key driver of belowground respiration processes but that photosynthate supply may control these processes in several ways.