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1.
Vet Microbiol ; 290: 109996, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38295490

RESUMO

Mycoplasma mycoides ssp. capri (Mmc) is one of the etiological microorganisms of contagious agalactia, which is among the diseases causing the highest economical losses in small ruminants. We report a disease outbreak in a German flock that led to significant suffering of goats characterized by mastitis, arthritis, pleuropneumonia and sudden deaths. Mmc was persistently isolated from many animals both from milk, and from a number of different swab and tissue samples. A number of closely related Mycoplasma spp. have to be taken into consideration to rule out important animal epizootics listed by European Animal Health Law and the World Organisation for Animal Health (WOAH). Some goats developed cross-reacting antibodies against Mycoplasma mycoides ssp. mycoides. Although Mmc is believed to be an uncommon microorganism in Germany, this study highlights that veterinarians should consider this pathogen in their work during herd health monitoring in Central Europe. Although eradication was not fully achieved, autogenous vaccination significantly seemed to improve animal health and welfare.


Assuntos
Doenças das Cabras , Mastite , Infecções por Mycoplasma , Mycoplasma mycoides , Mycoplasma , Pleuropneumonia Contagiosa , Feminino , Animais , Cabras , Infecções por Mycoplasma/epidemiologia , Infecções por Mycoplasma/veterinária , Pleuropneumonia Contagiosa/epidemiologia , Mastite/epidemiologia , Mastite/veterinária , Doenças das Cabras/epidemiologia
2.
Anim Genet ; 55(1): 152-157, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37921236

RESUMO

Microcephaly is a rare neurodevelopmental disorder characterized by reduced skull circumference and brain volume that occurs sporadically in farm animals. We investigated an early-onset neurodegenerative disorder observed in seven lambs of purebred Kerry Hill sheep. Clinical signs included inability to stand or severe ataxia, convulsions, and early death. Diagnostic imaging and brain necropsy confirmed microcephaly. The pedigree of the lambs suggested monogenic autosomal recessive inheritance. We sequenced the genome of one affected lamb, and comparison with 115 control genomes revealed a single private protein-changing variant. This frameshift variant, MFSD2A: c.285dupA, p.(Asp96fs*9), represents a 1-bp duplication predicted to truncate 80% of the open reading frame. MFSD2A is a transmembrane protein that is essential for maintaining blood-brain barrier homeostasis and plays a key role in regulating brain lipogenesis. Human MFSD2A pathogenic variants are associated with a neurodevelopmental disorder with progressive microcephaly, spasticity, and brain imaging abnormalities (NEDMISBA, OMIM 616486). Here we present evidence for the occurrence of a recessively inherited form of microcephaly in sheep due to a loss-of-function variant in MFSD2A (OMIA 002371-9940). To the best of our knowledge, this is the first report of a spontaneous MFSD2A variant in domestic animals.


Assuntos
Microcefalia , Doenças dos Ovinos , Simportadores , Humanos , Ovinos/genética , Animais , Microcefalia/genética , Microcefalia/veterinária , Microcefalia/metabolismo , Encéfalo/metabolismo , Barreira Hematoencefálica/metabolismo , Barreira Hematoencefálica/patologia , Mutação da Fase de Leitura , Carneiro Doméstico/genética , Carneiro Doméstico/metabolismo , Animais Domésticos/genética , Linhagem , Simportadores/genética , Doenças dos Ovinos/genética
4.
Emerg Infect Dis ; 29(3): 631-634, 2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-36823667

RESUMO

Lymphocytic choriomeningitis mammarenavirus (LCMV) is a globally distributed zoonotic pathogen transmitted by house mice (Mus musculus). We report the reemergence of LCMV (lineages I and II) in wild house mice (Mus musculus domesticus) and LCMV lineage I in a diseased golden lion tamarin (Leontopithecus rosalia) from a zoo in Germany.


Assuntos
Coriomeningite Linfocítica , Doenças dos Roedores , Animais , Camundongos , Vírus da Coriomeningite Linfocítica , Alemanha
5.
Antonie Van Leeuwenhoek ; 113(12): 2139-2154, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33150542

RESUMO

A number of different Chlamydia spp. have been detected in the class Amphibia with C. pneumoniae being the predominant species involved. Chlamydiae have been linked to mass mortality events, thereby representing significant pathogens that deserve attention with respect to worldwide amphibian decline. We here present six cases of chlamydiosis and asymptomatic chlamydial infections in different frog species from three ex situ amphibian conservation facilities. Clinical signs predominantly characterised by regurgitation, chronic wasting, lethargy and suspended breeding were associated with C. pneumoniae infection. Despite various treatment regimens, it was not possible to clear infections. However, intra vitam diagnostics succeeded from skin, faeces and urine for the first time.


Assuntos
Infecções por Chlamydia , Chlamydia , Chlamydophila pneumoniae , Humanos
6.
Med Mycol Case Rep ; 25: 39-44, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31428554

RESUMO

The genus Sporothrix contains both species pathogenic to humans and animals as well as environmental fungi. S. humicola, a member of the latter S. pallida clade, has previously been reported only from soil. We have isolated this dimorphic fungus from multiple cutaneous lesions in two endangered marsupials native to Tasmania. Clinical appearance resembled cutaneous sporotrichosis, highlighting the principle pathogenic potential. Identification was based on partial ITS, ß-tubulin and calmodulin gene sequencing.

7.
Virol J ; 13(1): 151, 2016 09 02.
Artigo em Inglês | MEDLINE | ID: mdl-27590473

RESUMO

BACKGROUND: Next to various known infectious and non-infectious causes, the aetiology of non-suppurative encephalitis in red foxes (Vulpes vulpes) often remains unclear. Known causes in foxes imply rabies, canine distemper, toxoplasmosis, Aujeszky's disease, as well as parvovirus, adenovirus, circovirus and flavivirus infections. In this study, particular attention was paid on bornaviruses, since red foxes are predators of bicoloured white-toothed shrews, a reservoir of Borna disease virus 1 (BoDV-1). In addition, foxes are known to be highly susceptible for viruses of the order Mononegavirales. METHODS: Analyses for the presence of anti-BoDV-1 antibodies, BoDV-1-RNA and antigen were performed on 225 blood and 59 brain samples, from a total of 232 red foxes. Foxes originated from BoDV-1 endemic and non-endemic German areas. Additional investigations for the presence of rabies, canine distemper, toxoplasmosis, Aujeszky's disease, parvovirus, adenovirus and flavivirus infections were carried out on 16 red foxes with non-suppurative (meningo-) encephalitis. A metagenomic analysis was used on three representative brain samples displaying encephalitis. RESULTS: Among 225 foxes, 37 displayed anti-BoDV-1 antibodies with titres ranging between 1:40 and 1:2560, regardless of geographic origin. In 6 out of 16 foxes with encephalitis, canine distemper virus was detected. No evidence of any of the other investigated agents was found in the 16 fox brains with encephalitis. Metagenomics revealed no infectious agents, except for one already known canine distemper case. CONCLUSION: Red foxes can exhibit BoDV-1 specific antibodies without association with geographic origin or encephalitis due to bornavirus infection. The encephalitis pattern was highly conspicuous for a viral infection, but remained unclear in 10 out of 16 foxes. Thus, presently unknown infectious and non-infectious causes need to be considered and further investigated, especially since foxes also tend to occur in human proximity.


Assuntos
Encefalite Viral/veterinária , Raposas/virologia , Vírus/classificação , Vírus/isolamento & purificação , Animais , Anticorpos Antivirais/sangue , Encéfalo/virologia , DNA Viral/sangue , Encefalite Viral/epidemiologia , Encefalite Viral/virologia , Feminino , Alemanha/epidemiologia , Programas de Rastreamento , Metagenômica , RNA Viral/isolamento & purificação , Vírus/genética , Vírus/imunologia
8.
Berl Munch Tierarztl Wochenschr ; 127(3-4): 115-9, 2014.
Artigo em Alemão | MEDLINE | ID: mdl-24693655

RESUMO

A complex of various malformations in newborns was observed to an increased extent in sheep farms in the 2011/2012 lambing season. An intrauterine Schmallenberg virus (SBV) infection was identified as the cause of these malformations. To date, a detailed pathological description of the deformity complex has only been given for bovine and ovine newborns.The aim of this study was therefore to provide a description of pathologic-anatomical congenital malformations in goat kids caused by intrauterine SBV infection. To this end, pathologic-anatomical and molecular biological investigations by PCR were carried out on 37 goat kids and 457 lambs from 238 sheep and goat farms in order to carry out an interspecies comparison. Of the 37 goat kids dissected, it was possible to identify a SBV infection in twelve animals (32.4%) by RT-PCR. In nine animals (24.3%) displaying pathological-anatomical malformations SBV could not be detected by PCR. The following malformations were observed: athrogryposis, deformation of spinal column, torticollis, asymmetry of the skull, brachygnathia inferior, cerebellar hypoplasia, cerebellar aplasia and internal hydrocephalus. Arthogryposis was the most common malformation, both in animals with positive PCR results and those with negative PCR results. This study documents congenital malformations caused by an intrauterine SBV infection for the first time on a large number of newborn goats.


Assuntos
Infecções por Bunyaviridae , Doenças das Cabras/patologia , Doenças das Cabras/virologia , Orthobunyavirus/isolamento & purificação , Complicações Infecciosas na Gravidez , Animais , Animais Recém-Nascidos , Artrogripose/patologia , Artrogripose/veterinária , Artrogripose/virologia , Infecções por Bunyaviridae/patologia , Infecções por Bunyaviridae/veterinária , Infecções por Bunyaviridae/virologia , Feminino , Cabras/virologia , Orthobunyavirus/genética , Reação em Cadeia da Polimerase , Gravidez , Complicações Infecciosas na Gravidez/patologia , Complicações Infecciosas na Gravidez/veterinária , Complicações Infecciosas na Gravidez/virologia , Doenças da Coluna Vertebral/patologia , Doenças da Coluna Vertebral/veterinária , Doenças da Coluna Vertebral/virologia
9.
BMC Microbiol ; 13: 61, 2013 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-23517149

RESUMO

BACKGROUND: Tularemia is a zoonotic disease caused by Francisella tularensis that has been found in many different vertebrates. In Germany most human infections are caused by contact with infected European brown hares (Lepus europaeus). The aim of this study was to elucidate the epidemiology of tularemia in hares using phenotypic and genotypic characteristics of F. tularensis. RESULTS: Cultivation of F. tularensis subsp. holarctica bacteria from organ material was successful in 31 of 52 hares that had a positive PCR result targeting the Ft-M19 locus. 17 isolates were sensitive to erythromycin and 14 were resistant. Analysis of VNTR loci (Ft-M3, Ft-M6 and Ft-M24), INDELs (Ftind33, Ftind38, Ftind49, RD23) and SNPs (B.17, B.18, B.19, and B.20) was shown to be useful to investigate the genetic relatedness of Francisella strains in this set of strains. The 14 erythromycin resistant isolates were assigned to clade B.I, and 16 erythromycin sensitive isolates to clade B.IV and one isolate was found to belong to clade B.II. MALDI-TOF mass spectrometry (MS) was useful to discriminate strains to the subspecies level. CONCLUSIONS: F. tularensis seems to be a re-emerging pathogen in Germany. The pathogen can easily be identified using PCR assays. Isolates can also be identified within one hour using MALDI-TOF MS in laboratories where specific PCR assays are not established. Further analysis of strains requires genotyping tools. The results from this study indicate a geographical segregation of the phylogenetic clade B.I and B.IV, where B.I strains localize primarily within eastern Germany and B.IV strains within western Germany. This phylogeographical pattern coincides with the distribution of biovar I (erythromycin sensitive) and biovar II (erythromycin resistance) strains. When time and costs are limiting parameters small numbers of isolates can be analysed using PCR assays combined with DNA sequencing with a focus on genetic loci that are most likely discriminatory among strains found in a specific area. In perspective, whole genome data will have to be investigated especially when terrorist attack strains need to be tracked to their genetic and geographical sources.


Assuntos
Francisella tularensis/classificação , Francisella tularensis/genética , Variação Genética , Lebres/microbiologia , Doenças dos Roedores/microbiologia , Tularemia/veterinária , Estruturas Animais/microbiologia , Animais , Antibacterianos/farmacologia , Análise por Conglomerados , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Farmacorresistência Bacteriana , Eritromicina/farmacologia , Francisella tularensis/isolamento & purificação , Genótipo , Alemanha , Testes de Sensibilidade Microbiana , Repetições Minissatélites , Tipagem Molecular , Filogeografia , Reação em Cadeia da Polimerase , Tularemia/microbiologia
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