[Electron microscopic observation on cysticercus cellulosae treated with mienangling in vitro].

OBJECTIVE: To observe the effect of Mienangling (MNL) in treating cysticercus cellulosae in vitro. METHODS: The changes of cysticercus cellulosae treated in vitro with the MNL extracts, praziquantel (PQT) and albendazole (ALB) were studied under the transmission and scanning electron microscopes. RESULTS: Under the scanning electron microscope, the authors can see that all of the cysticercus's scoleces did not grow in the MNL and PQT groups, cyst walls shrivelled, tegument's ectoplasts were damaged, microtriches's ultrastructures were not clear or exfoliated, some corpuscular matter densely was distributed in the surface; 46.7% of cysticercus cellulosaes in the ALB group had been excystated, the rostellar hooks of rostella developed normally, four sucker's surface wrer hollow, the necks were eroded, and grooves of sonite disappeared. Under the transmission electron microscope, we can see that the tegument, basement membrane, muscular layer and parenchyma in the groups of MNL and PQT were all clearly damaged, but the harmful effect of MNL to parenchyma's nerve cords is more serious than the PQT. The damage of all layers of the cysticercus cellulosae in the ALB group is smaller in degree than the MNL and the PQT. CONCLUSION: The MNL has marked damaging effect to cysticercus cellulosae.

A neuron model based on brain biochemical principles.

The electrical activity and excitation of the brain is based on certain biochemical processes, particularly, the activity of transmitter-receptor-ion channel system and the DNA-RNA-Pr (Pr:protein) system. There have already been some neuron models relating to the ion channel, but the kinds and numbers of transmitters, receptors, and ion channels are directly or indirectly conditioned by the DNA-RNA-Pr system. To reflect these facts, we present a new neuron model based on the important biochemical chains of the brain. This model is applied to both the simulation research of electrical activity and the information processing function of the brain. In this demonstration, we will introduce: 1) How to express the connection between the neuron excitation and the transmitter-receptor-ion channel system; 2) How to express the connection between the transmitter-receptor-ion channel system of neuron and the DNA-RNA-Pr system; 3) How to express the connection among 16,000 artificial neurons on the basis of the above-mentioned points; 4) The time and the state of the excitation passing through a particular neuron in a group of neurons.

[Changes of auditory brainstem response and auditory cortex response after exposure to intensive noise].

Auditory brainstem response and auditory cortex response were recorded repeatedly in 35 guinea pigs after exposure to intensive white noise (125 dB, 150 min.) for 62 d. the amplitude of evoked potential of acoustic nerve was decreased by 29% (P < 0.05), of the cochlear nuclei by 28% (P < 0.05). However, the amplitude of response of superior olives nuclei was increased by 21% (P < 0.05), of the inferior colliculi by 37% (P < 0.05), of the cortical evoked response by 131% (P < 0.001). The results indicate that the amplitudes of auditory evoked potential showed a centripital augmentation after exposure to intensive noise. The centripital augmentation was observed not only during the period of the temporary threshold shift (TTS) but also during that of the permanent threshold shift (PTS).

[Clinical study in treating intractable ulcerative colitis with traditional Chinese medicine].

UNLABELLED: Clinical double blind study in treating 153 intractable ulcerative colitis with Chinese medicinal herbs was conducted, the patients were randomly assigned to three groups. Group I is administered with Jian Pi Ling (JPL) tablet with retention-enema of Radix Sophorae Flavescentis and Flos Sophora (RSF-FS) decoction per night, group II with salicylazosulfapyridine (SASP) and retention-enema of dexamethasone, group III with placebo and retention-enema of decoction as that in group I. After 90 days every patients were checked by means of fibro-enteroscope, pathologic and immunologic parameters. THE RESULTS: the curative rates of group I, II, III, were 53.1%, 27.7% and 19.0%, the total effective rates were 85.9%, 59.6% and 45.2% respectively. By comparison among groups, the efficacy of group I was the best (P < 0.01). The check of T and B lymphocyte subpopulation showed the B lymphocyte of group I markedly decreased, OKT3 and OKT8 obviously increased, the ratio of OKT4 and OKT8 approached normal value. The amount of IgG, IgM, C3 increased abnormally, decreased dramatically after medication, while those of group II and III have not changed significantly. The bacteriostatic test in vitro showed the bacteriostatic effect of RSF-FS on pathogenic B. coli, Shigella dysenteriae and Staphylococcus aureus was the best, that of solution Jian Pi Ling the next, that of SASP was the least effective. Therefore, the principle and method of group I seems to be the best therapeutic programme.

[Duplication of animal model of ulcerative colitis and studies on rehabilitation effect of jianpiling prescription].

In this paper, the ulcerative colitis model of rats with immuno-method was reported and the mechanism of using Jianpiling for the treatment of ulcerative colitis was studied. The rats of experimental group were subdivided into 3 subgroups (A, B and C), 15% Jianpiling suspension was given to rats in subgroup A per os, 15% Bupi-Yichang Pill to rats in subgroup B per os, 4.5% SASP suspension to rats in subgroup C, and same amount of saline to rats in the control group. The therapeutic results showed that the anti-diarrhia, anti-infection and repairing function of tissue by Jianpiling were obviously better than those of Bupi-Yichang Pill and SASP. Serological exams also showed that Jianpiling was much better than other therapeutic groups in increasing nonspecific cellular immunity and lowering level of antibody against large intestine and IgG. These results suggested that the mechanism of Jianpiling on ulcerative colitis was realized by increasing cellular immunity, inhibiting humoral immunity and promoting immune modulation.