Surface characterization and frictional force between stainless steel brackets and archwires in orthodontic patients using chlorhexidine- and Persica-containing mouthrinses: A randomized controlled trial.

BACKGROUND: The aim of this study was to compare the effect of chlorhexidine- and Persica-containing mouthrinses on the surface characterization of orthodontic appliance and friction between the orthodontic stainless steel wires and brackets. MATERIALS AND METHODS: In this randomized controlled trial, 75 orthodontic patients (aged 13-30) were allocated (n = 25) into two experimental groups (prescribed by Persica- or chlorhexidine-containing mouthrinse) and one control group (no prescription). The ovoid stainless steel archwires were placed, and the maxillary first premolar stainless steel edgewise brackets were ligated to wires by elastomeric rings. The patients were recalled after 2 weeks, and then, the archwires were removed and replaced. The surface analysis of archwires and brackets was evaluated using scanning electron microscopy and atomic force microscopy. The frictional forces between the archwires and brackets were measured using a universal testing machine. The data for surface roughness were analyzed by the nonparametric Kruskal-Wallis and Mann-Whitney tests. The frictional forces data were analyzed using a two-way analysis of variance and Tukey's post hoc test using SPSS software. The level of significance was P < 0.05. RESULTS: The retrieved brackets showed minor changes in their surface topography. The surface roughness of archwires after the intervention was significantly greater for the chlorhexidine than that of Persica (P < 0.05). The friction force between the archwires and brackets was also significantly higher for the chlorhexidine than that of Persica (P < 0.05). CONCLUSION: The Persica-containing mouthrinse from the biomechanical and biochemical standpoints may be a better option for oral hygiene in orthodontic patients compared with the chlorhexidine.

In vitro evaluation of shear bond strength of orthodontic metal brackets to aged composite using a self-adhesive composite: Effect of surface conditioning and different bonding agents.

OBJECTIVES: Bracket bonding to composite surfaces is increasing. This study sought to assess the effect of different adhesives (acid etchant+Transbond XT™, acid etchant+Vertise Flow™, Vertise Flow™) with different surface treatments (no preparation, bur, sandblasting, Er:YAG laser) on shear bond strength (SBS) of metal brackets to aged composite. MATERIALS AND METHODS: In this in vitro, experimental study, Filtek™ Z250 composite discs were fabricated (10×4mm). After thermocycling (10,000 cycles between 5-55°C), the samples were randomly divided into three groups (n=48) based on the type of adhesive namely acid etchant+Transbond XT™, Vertise Flow™, and acid etchant+Vertise Flow™. Samples in each group were randomly divided into four subgroups (n=12) based on the surface treatment including no-treatment, diamond bur, sandblasting and Er:YAG laser. Samples in each group were randomly divided into three subgroups (n=12) based on the type of adhesive namely acid etchant+Transbond XT™, Vertise Flow™, and acid etchant+Vertise Flow™. After bracket bonding to composite discs, the samples underwent thermocycling (5000 cycles between 5-55°C), and their SBS was measured in a universal testing machine. The samples were then inspected under a stereomicroscope at x10 magnification to determine their mode of failure and adhesive remnant index (ARI). RESULTS: No significant difference was noted in SBS between adhesives in no-preparation and laser groups (P>0.05). In all adhesive groups, sandblast had highest SBS, but it was not significantly different in Vertise Flow™ groups, with and without etching. The SBS provided by acid etchant+Transbond XT™ was significantly higher than that of the other two adhesives (P<0.05). The SBS of sandblasted samples was significantly higher compared with other surface treatments in all adhesive subgroups (P<0.05). CONCLUSION: Bracket bonding to aged composite using Vertise Flow™ with or without surface treatment can serve as an alternative to the conventional orthodontic adhesives.

Effect of 0.05% Sodium Fluoride Mouthwash on Surface Roughness and Friction between Ceramic Brackets and Rhodium-Coated and Uncoated Stainless Steel Wires.

OBJECTIVES: This study aimed to assess the effect of 0.05% sodium fluoride (NaF) mouthwash on the surface roughness and friction between ceramic brackets and rhodium-coated (RC) and uncoated stainless steel (SS) wires. MATERIALS AND METHODS: This experimental study was performed on 48 maxillary premolar ceramic brackets. Twenty-four pieces of RC-SS wires were used. Samples were divided into four groups. Groups 1 and 2 were immersed in artificial saliva, and groups 3 and 4 were immersed in a solution consisting of artificial saliva (9%) and mouthwash (91%). To assess surface roughness, images were obtained from the surface of wires and brackets with atomic force microscopy (AFM) and scanning electron microscopy (SEM) before and after the intervention. To assess friction, the wires were ligated into brackets, and friction was measured at a crosshead speed of 0.5 mm/minute using a universal testing machine. Data were analyzed using one-way analysis of variance (ANOVA) at the 0.05 significance level. RESULTS: Friction during sliding in RC wires was significantly less than that in SS wires (P<0.05). Increase in the friction in SS wires by mouthwash was significantly greater compared to RC wires (P<0.05). Surface roughness coefficients of the wires before the intervention were not significantly different. The surface roughness of the wires significantly increased after the intervention and it was greater in SS wires than in RC wires (P<0.05). CONCLUSION: Considering the lower friction and surface roughness of SS-RC wires compared to SS wires, SS-RC wires may be a better alternative for use with ceramic brackets.

Comparison of residual monomer loss from cold-cure orthodontic acrylic resins processed by different polymerization techniques.

INTRODUCTION: This investigation aimed to assess and compare the amount of residual monomer (RM) released from removable orthodontic appliances constructed by sprinkle-on and dough techniques. MATERIALS AND METHODS: One hundred and twenty acrylic samples were prepared from orthodontic autopolymerized acrylic resins and divided into three groups, according to the processing method: sprinkle-on with polyclave, sprinkle-on without polyclave and dough technique. After polymerization, the specimens of each group were immersed in distilled water for 24 h, 48 h, 72 h and 1 week. High-performances liquid chromatography (HPLC) was utilized to measure residual monomer content. RESULTS: Maximum observed RM was 1284·91±129·07 ppm measured for sprinkle-on technique without polyclave after 24 h of water immersion. At this time, the level of RM was significantly different among the three applied techniques (P<0·05). In all soaking time groups, sprinkle-on technique with polyclave released the least amount of RM. Within each group, the maximum monomer releasing was observed after the first 24 h and decreases were observed in subsequent time groups. The reduction over the time was not significant in the polyclave groups (P>0·05). CONCLUSION: The sprinkle-on technique with polyclave and longer water immersion reduced residual monomer released from acrylic orthodontic appliances.

Long-term average spectra of adult Iranian speakers' voice.

INTRODUCTION: Long-term average spectrum (LTAS) allows quantifying the voice quality and provides an overview of the mean spectral characteristics of a voice. The aims of this study were to survey normal spectral characteristics of Persian and investigate sex-related changes in the source characteristics of dynamic speech using LTAS. METHOD: Speech samples obtained from 30 male and 30 female Persian-speaking participants reading a text in habitual pitch and loudness level. At the LTAS window and using Praat software, the amplitude values were obtained at equal intervals of 160 Hz, ranging from 0 to 8 kHz. RESULTS: The main features of the average spectrum were as follows: peak in the region of 480 Hz with a reduction at higher frequencies, a 20 dB decline from 480 to 960 Hz, a flat region from 960 to 1920 Hz, a further decline from 1920 to 3040 Hz, and a further flat region from 3040 to 8000 Hz. In comparison to men, women revealed significant lower levels of amplitude at frequencies of 160 and 320 Hz and higher levels of amplitude at frequencies of 960, 3360, 3520, 3680, 3840, and 5920 Hz. CONCLUSION: The overall shape and gender-related energy distribution pattern of the LTAS of Persian were more similar to those of English than to those of Korean. The more phonetic differences between Persian and Korean compared with Persian and English might contribute to different spectral characteristics. The present study tried to clarify the spectral characteristics of Iranian male and female voices and focused on more breathy voice quality for women than men.

Designing Orthodontic Craniofacial Templates for 8-14 year-old Iranian Girls Based on Cephalometric Norms.

OBJECTIVES: Cephalometry and its related analyses have an important role in the evaluation of orthodontic patients. Access to an analysis that gives maximum information in the least possible time is an effective way to indicate craniofacial disharmony; therefore, craniofacial templates are very useful tools. The purpose of the present study was to provide orthodontic craniofacial templates for 8-14-year-old Iranian girls. MATERIALS AND METHODS: One thousand two-hundred and nine girls (age range, 8-14 years) were examined. Eighty of these cases were finally chosen for the study and their lateral cephalograms were traced. Both Basion-Nasion (Ba-N) and Sella-Nasion (S-N) lines were selected for superimposition in this study. Based on these two mentioned lines, a template for each age was designed. Simple linear regression and multivariant regression analysis were used to evaluate the angles and to landmark the vectors, respectively. RESULTS: Findings show that most points change significantly at different ages in the S-N method. In the Ba-N method, all points except for S and Ba have significant changes at different ages. CONCLUSION: Templates that resulted from both methods were the same and alteration in the reference line and points does not change the total form of the average tracings of each age.

A comparison between craniofacial templates of Iranian and western populations.

Templates are very useful tools for diagnosis of malocclusions. A number of templates have been provided for some populations in previous years. Since craniofacial characteristics of different ethnic groups are not the same, each population needs its own norms. The aim of this study was to provide orthodontic craniofacial templates for 8-16 year old Iranian boys and compare dentoskeletal features between Iranian and western populations. 3330 boys with the age range of 8-16 years were examined in Tehran, and 107 cases were finally chosen for the study and their lateral cephalograms were traced. Since there is no universal, consensus about the selection of one specific point or line for cephalometric superimposition, both the sella nasion (SN) and basion nasion (Ba-N) lines were chosen for this purpose. Based on both SN and Ba-N lines, a template was prepared for each age. Simple linear regression analysis was used to evaluate the angles and the multivariant regression analysis for evaluation of landmark vectors. Posterior cranial base, maxillary and mandibular lengths, upper and lower anterior facial heights (N-ANS and ANS-Me) and posterior facial height (S-Go) are greater in Iranian population. But anterior cranial base, height and inclination of the incisors and molar height are similar in two populations.

Effects of continuous and interrupted forces on gene transcription in periodontal ligament cells in vitro.

The biological mechanisms of tooth movement are based on the response of periodontal tissues to mechanical forces. The final result of these responses is remodeling of the extracellular matrix. Tissue reactions may vary depending upon the type, magnitude and duration of the applied forces. The purpose of the present study was to analyze the effects of centrifugal force on the transcription of collagen type-I (Col-I), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinase- 1 (TIMP-1) genes in human periodontal ligament (PDL) fibroblasts. Human fibroblasts obtained from the PDL were cultured and subjected to centrifugal forces (36.3 g/cm(2)) for 30, 60 and 90 min continuously. This was also carried out interruptedly, three times for 30 min and six times for 15 min. The mRNAs encoding for Col-I, MMP-1, and TIMP-1 were quantified using RT-PCR. The mRNA levels of Col-I and MMP-1 were increased when continuous force was applied for 30 min and 60 min respectively. The interrupted force had almost no effect on Col-I, MMP-1 and TIMP-1 genes. These results indicate that continuous forces may have a greater effect in inducing gene expression during the remodeling process of PDL compared to interrupted forces with short rest periods.

Comparison of debonding characteristics of metal and ceramic orthodontic brackets to enamel: an in-vitro study.

INTRODUCTION: The objectives of this study were to compare the debonding strengths of 1 metal and 2 types of ceramic orthodontic brackets with different retention mechanisms bonded to enamel and to determine the risk of enamel damage after debonding. METHODS: In this interventional in-vitro study, 36 maxillary premolars were divided into 3 groups. Three types of orthodontic brackets (metal, ceramic with chemical retention, and ceramic with mechanical retention) were bonded to the teeth with a luting resin composite. The brackets were debonded with a sharp-edged debonding pliers in a universal testing machine. Enamel cracks were evaluated with a stereomicroscope. The amount of residual adhesive on the enamel surfaces was evaluated with the adhesive remnant index. RESULTS: The mean bond strength for the metal brackets was significantly higher than that of the 2 ceramic brackets (P <.001). No significant difference between the mean bond strengths for the 2 ceramic brackets was observed (P = .238). There was no statistically significant difference in the number (P = .871) or length (P = .188) of enamel cracks among the 3 groups. There were significant differences in the adhesive remnant index scores between metal and chemically retained ceramic brackets (P = .007), and between chemically and mechanically retained ceramic brackets (P = .002). CONCLUSIONS: The risk of enamel damage when debonding ceramic brackets is not greater than the risk when debonding metal brackets.