Augmentation of bacterial homeostasis by regulating in situ buffer capacity: Significance of total dissolved salts over acidogenic metabolism.

During anaerobic fermentation, consequent accumulation of acidic fermented products leads to the failure of pH homeostasis. The present study aimed to comprehend the changes in buffering capacity with addition of sodium salts of hydroxide, bicarbonate and phosphate. The results showed notable augmentation in buffer capacity and cumulative hydrogen production (CHP) compared to control. The influential factor is the amount of undissociated volatile fatty acids released that affected the cell metabolism and consequently biohydrogen generation. It is inferred that among the tested salts, sodium bicarbonate has substantial buffering capacity (ß, 0.035± mol) ensuing maximum CHP (468± mL). Besides, bioelectrochemical analysis revealed variations in redox currents that aligned with biohydrogen production. The study provides valuable information on the role of inorganic dissolved salts that would be required to regulate H2 generation and acidogenesis in the aspects of acid-gas phase system.

Assessing potential cathodes for resource recovery through wastewater treatment and salinity removal using non-buffered microbial electrochemical systems.

The present study evaluates relative functioning of microbial electrochemical systems (MES) for simultaneous wastewater treatment, desalination and resource recovery. Two MES were designed having abiotic cathode (MES-A) and algal biocathode (MES-B) which were investigated with synthetic feed and saline water as proxy of typical real-field wastewater. Comparative anodic and cathodic efficiencies revealed a distinct disparity in both the MES when operated in open circuit (OC) and closed circuit (CC). The maximum open circuit voltage (OCV) read in MES-A and MES-B was about 700mV and 600mV, respectively. Salinity and organic carbon removal efficiencies were noticed high during CC operation as 72% and 55% in MES-A and 60% and 63% in MES-B. These discrete observations evidenced ascribe to the influence of microbial electrochemical induced ion-migration over cathodic reduction reactions (CRR).

Waste biorefinery models towards sustainable circular bioeconomy: Critical review and future perspectives.

Increased urbanization worldwide has resulted in a substantial increase in energy and material consumption as well as anthropogenic waste generation. The main source for our current needs is petroleum refinery, which have grave impact over energy-environment nexus. Therefore, production of bioenergy and biomaterials have significant potential to contribute and need to meet the ever increasing demand. In this perspective, a biorefinery concept visualizes negative-valued waste as a potential renewable feedstock. This review illustrates different bioprocess based technological models that will pave sustainable avenues for the development of biobased society. The proposed models hypothesize closed loop approach wherein waste is valorised through a cascade of various biotechnological processes addressing circular economy. Biorefinery offers a sustainable green option to utilize waste and to produce a gamut of marketable bioproducts and bioenergy on par to petro-chemical refinery.

Closed circuitry operation influence on microbial electrofermentation: Proton/electron effluxes on electro-fuels productivity.

A novel biocatalyzed electrofermentor (BEF) was designed which uncovers the intricate role of biocatalyst involved in cogeneration of electro-fuels (hydrogen and electricity). The specific role of external resistance (Rext, electrical load) on the performance of BEF was evaluated. Four BEFs were operated separately with different resistances (25, 50, 100 and 200 Ω) at an organic load of 5 g/L. Among the tested conditions, external resistance (R3) with 100 Ω revealed maximum power and cumulative H2 production (148 mW and 450 mL, respectively). The competence of closed circuitry comparatively excelled because it facilitates congenial ambiance for the enriched EAB (electroactive bacteria) resulting high rate of metabolic activity that paves way for higher substrate degradation and electro-fuel productivity. Probing of electron kinetics was studied using voltammetric analyses wherein electron transfer by redox proteins was noticed. The designed BEF is found to be sustainable system for harnessing renewable energy through wastewater treatment.

Single-Stage Operation of Hybrid Dark-Photo Fermentation to Enhance Biohydrogen Production through Regulation of System Redox Condition: Evaluation with Real-Field Wastewater.

Harnessing hydrogen competently through wastewater treatment using a particular class of biocatalyst is indeed a challenging issue. Therefore, biohydrogen potential of real-field wastewater was evaluated by hybrid fermentative process in a single-stage process. The cumulative hydrogen production (CHP) was observed to be higher with distillery wastewater (271 mL) than with dairy wastewater (248 mL). Besides H2 production, the hybrid process was found to be effective in wastewater treatment. The chemical oxygen demand (COD) removal efficiency was found higher in distillery wastewater (56%) than in dairy wastewater (45%). Co-culturing photo-bacterial flora assisted in removal of volatile fatty acids (VFA) wherein 63% in distillery wastewater and 68% in case of dairy wastewater. Voltammograms illustrated dominant reduction current and low cathodic Tafel slopes supported H2 production. Overall, the augmented dark-photo fermentation system (ADPFS) showed better performance than the control dark fermentation system (DFS). This kind of holistic approach is explicitly viable for practical scale-up operation.

Applied potentials regulate recovery of residual hydrogen from acid-rich effluents: Influence of biocathodic buffer capacity over process performance.

An absolute biological microbial electrolysis cell (MEC) was operated for a prolonged period under different applied potentials (Eapp, -0.2V to -1.0V) and hydrogen (H2) production was observed using acid-rich effluent. Among these potentials, an optimal voltage of -0.6 V influenced the biocathode by which maximum H2 production of 120 ± 9 ml was noticed. This finding was corroborated with dehydrogenase activity (1.8 ± 0.1 µg/ml) which is the key enzyme for H2 production. The in situ biocathode regulated buffer overpotentials which was remarkably observed by the change in peak heights of dissociation value (pKa) from the titration curve. Substrate degradation analysis gave an estimate of coulombic efficiency of about 72 ± 5% when operated at optimal voltage. Evidently, the electron transfer from solid carbon electrode to biocathode was analyzed by cyclic voltammetry and its derivatives showed the involvement of redox mediators. Despite, the MEC endures certain activation overpotentials which were estimated from the Tafel slope analysis.

Systematic approach to assess biohydrogen potential of anaerobic sludge and soil rhizobia as biocatalysts: Influence of crucial factors affecting acidogenic fermentation.

A systematic protocol was designed to enumerate the variation in biohydrogen production with two different biocatalysts (sludge and soil) under different pH and organic loads. Both the biocatalysts showed cumulatively higher H2 production under acidogenic condition (pH 6) than at neutral pH condition. The cumulative hydrogen production was non-linearly fitted with modified Gompertz model and statistically validated. Pretreated soil biocatalyst showed relatively higher H2 production (OLR II, 142±5ml) than pretreated sludge (OLR I, 123±5ml); which was evidenced by substrate linked dehydrogenase activity and bio-electrochemical analysis. Experimental results revealed agricultural soil as a better biocatalyst than anaerobic sludge for all the operated process conditions. The voltammogram profiles and Tafel slopes revealed dominance of reductive catalytic activity of the pretreated inoculums substantiating dark-fermentation. Soil consortia showed low polarization resistance (2.24kΩ) and high reductive electron transfer efficiency (1.17 Vdec(-1)) at a high organic load; thus, rebating high H2 production.

Pseudomonas otitidis as a potential biocatalyst for polyhydroxyalkanoates (PHA) synthesis using synthetic wastewater and acidogenic effluents.

Polyhydroxyalkanoates (PHA) production using Pseudomonas otitidis, a newly isolated strain from PHA producing bioreactor was investigated using synthetic acids (SA) and acidogenic effluents (AE) from biohydrogen reactor at different organic loading rates (OLRs). P. otitidis showed ability to grow and accumulate PHA, with simultaneous waste remediation. AE showed less PHA production (54%, OLR3), than SA (58%, OLR2). PHA composition showed co-polymer, poly-3(hydroxy butyrate-co-hydroxy valerate), P3(HB-co-HV). Bioprocess evaluation and enzymatic activities showed good correlation with PHA production. Kinetic studies on the growth of bacteria using different models at varying OLR were substantiated with PHA production. High substrate removal was registered at OLR1 (SA, 87%; AE, 82%). AE could be used as an alternative for pure substrates keeping in view of their high cost.