[The gastroprotective action of acidic mineral water from the Azhyg-Sug source on the stress-induced injuries to the stomach of white rats]. / Gastroprotektivnoe deistvie kisloi mineral'noi vody istochnika Azhyg-Sug pri stress-indutsirovannom povrezhdenii zheludka u belykh krys.

BACKGROUND: The relevance of this study is determined by the need for the scientifically sound substantiation of the possibility for the use of acidic mineral waters in the treatment of digestive diseases taking into consideration their widespread application in ethnomedicine, in particular in the Republic of Tuva. AIM: The objective of the present study was to elucidate the gastroprotective action of acidic mineral water from the Azhyg-Sug source (Republic of Tyva) on the experimental animals as exemplified by the model of ulcerogenesis under the conditions of immobilization stress. MATERIAL AND METHODS: The experimental studies were carried out with the use of 32 white rats of the Wistar line. Ulcerogenesis was initiated by means of immobilization stress. We evaluated the pathomorphological characteristics of the gastric mucosa and the number of destructions based on the Pauls index. The state of the lipid peroxidation system and antioxidative protection were determined from the content of malon dialdehyde and reduced glutathione, extracellular catalase and superoxide dismutase activities in erythrocytes. RESULTS: The study has demonstrated that the mineral water from the Azhyg-Sug source slows down the development of the inflammatory and destructive necrotic processes in the mucosa of the stomach of albino rats. The depth of erosion in the animals receiving mineral water was 2.3 and 3.4 times lower than in the control animals (p≤0.05). The antioxidant effect of mineral water was confirmed by the 14-20% decrease of the MDA concentration as well as by the increase of the catalase and superoxide dismutase (SOD) activities by 21-25% and 20-30% respectively in comparison with the control animals. CONCLUSION: The Azhyg-Sug mineral water has the strong gastroprotective influence on the experimental animals having the induced neurogenic ulcer. One of the mechanisms underlying the gastroprotective action of the investigated mineral water arises from its ability to inhibit the processes of lipid peroxidation with the simultaneous enhancement of the activity of the antioxidant system of the organism.

[Treatment of chronic viral hepatitis C with direct acting antiviral agent: review].

HCV infection treatment regimens are viewed from positions of HCV life cycle and replication, effects of NS3/4A protease inhibitors and NS5A/NS5B inhibitors on HCV strain replication. Evolution of HCV treatment regimens from its discovery to modern DAA agents had led to substantial progress although drug resistance poses a new issue to be addressed.

[Antimutagenic and antioxidant features of confectionery products containing the powder from the leaves of Hippophae rhamnoides L.]

The aim of the work was to determine the possibility of using the powder from the leaves of Hippophae rhamnoides L. for enriching flour confectionery and to evaluate the antimutagenic and antioxidant activity of the product. The experiment was carried out on 24 white Wistar rats with initial body weight (b.w.) 180-200 g. The animals of the experimental group (n=8) received confection containing sea buckthorn powder at a rate of 20 mg per 100 g b.w. for 14 days on the background of a standard vivarium diet. The animals of the control and intact groups received confection containing no bioactive supplement at the same dose. Antimutagenic and antioxidant effects were estimated in a day after a single injection of cyclophosphamide at a dose of 20 mg/kg b.w. The number of chromosomal aberrations in bone marrow cells of white rats was counted and the activity of catalase, superoxide dismutase (SOD), the level of reduced glutathione and the concentration of TBA-active products in blood were evaluated. The intake of the confectionery containing the powdered H. rhamnoides leaves resulted in the 45% decrease of the number of damaged cells, 50% decrease of the proportion of cells with multiple chromosome breaks and 52% decrease of the number of achromatic gaps as compared to animals of the control group (n=8). The cake intake increased the activity of catalase (by 52%) and SOD (by 33%) and glutathione content (by 26%) in blood.

[Hepatoprotective effect of Hypecoum erectum extract on experimental D-galactosamine-induced damage of rat liver].

Hepatoprotective properties of the extract derived from the herbs of Hypecoum erectum L. have been studied on a model of D-galactosamine-induced hepatitis in rats. It is established that Hypecoum erectum extract in a dose of 50 mg/kg diminishes the development of cytolysis and cholestasis syndromes, as manifested by maximum decrease in the following indices after 7 days of the experiment: ALT activity by 26%; AST activity by 44%; alkaline phosphatase activity by 30%; ß-lipoproteins by 21%; and bilirubin by 29% (p < 0.05). The Hypecoum erectum extract (i) increases the energy potential of hepatocytes, manifested by increasing the ATP content by 70% (p = 0.001) and normalizing the ratio of lactate and pyruvate in the liver homogenate; (ii) inhibits lipid peroxidation, manifested by decreasing the content of malonic dialdehyde in the liver homogenate and diene conjugates in the blood serum on the average by 30% (p < 0.05); (iii) activates the antioxidant system of the organism, increasing the catalase activity in liver homogenates by 58% (p < 0.05) and 11% and the content of reduced glutathione in the blood by 56% (p < 0.05) and 36% (p < 0.05), respectively, on the 3rd and 7th days of the experiment.

[Effect of Hypecoum erectum extract on morphofunctional state of the liver in rats with tetracycline-associated hepatitis].

The effect of Hypecoum erectum L. extract on the morphofunctional condition of the liver in rats with experimental tetracycline-associated hepatitis was studied. The experiment included 40 albino rats Wistar. The animals treated with tetracycline hydrochloride (1.0 g/kg body weight) were exposed to the extract in a dose of 50 mg/kg for 5 days. On the 7th day of the experiment the following indices were determined: malonic dialdehyde concentration, catalase activity, the levels of ATP, pyruvate and lactate in the liver homogenate, as well as the blood levels of reduced glutathione. The liver pathomorphological investigation was applied. The H. erectum extract was shown to inhibit lipid peroxidation, to increase the activity of the host endogenous antioxidant system, to normalize the hepatocyte energy provision and to limit the liver degeneration.

[The choleretic and hepatoprotective effect of Hypecoum erectum extract].

The choleretic and hepatoprotective effect of Hypecoum erectum L. dry extract on toxic hepatitis was studied. Experimental hepatitis was caused by the introduction of D-galactosamine to Wistar white rats in the dose of 500 mg/kg of the animal weight once a day for 3 days. The H. erectum extract was administered per os in the dose of 50 mg/kg for 7 days. It has been established that H. erectum extract has a marked hepatoprotective effect in the case of D-galactosamine hepatitis in white rats that is characterized by inhibition of the disturbances in cholate-synthetic functions of the liver, increase of bile secretion rate, preservation of cholate concentration in the bile. The tested remedy diminishes dystrophic and necrotic processes, decreases the intensity of inflammatory infiltration in the liver and stimulates regeneration of liver cells in D-galactosamine hepatitis.

[Effect of Leptopyrum fumarioides (Ranunculaceae) extract on choleresis in white rats with toxic hepatitis].

It was established pharmacotherapeutic efficiency of Leptopyrum fumarioides (L.) Reichenb. dry on the toxic hepatitis model. The Leptopyrum fumarioides extract was shown expressed caused of inhibiting of cholerforming and choleretic function of liver and their destructive changes.

[Anti-inflammatory effect of seven-component herbal tea "Narkophyt"].

Experiments showed that a seven-component herbal mix decoction Narkophyt possesses anti-inflammatory properties, as manifested by the inhibition of exudation, reduction of the permeability of blood vessels, and decrease of fat cells degranulation.

[Technology of production and biological activity of toothpaste from Rubus idaeus].

Composition and the technology of manufacturing of toothpaste from extract of blackberry with anti-inflammatory activity have been developed. Toothpaste contains the following ingredients (g): Blackberry extract - 3.0 5.0 7.0 Aluminum hydroxide - 30,0 Glycerin - 10,0 Sodim carboxymethylcellulose - 1,5 Sodium laurilsulfate - 2,0 Calcium glycerophosphate - 1,5 Sodium monofluorophosphate - 1.0 Titanium dioxide - 1,0 Perfume - 1,0 Sorbit - 1,0 Purified water to 100 ml. Toothpaste containing 3%, 5% and 7% of blackberry extract possesses higher antioedemic activity and has mild influence on alteration and proliferation processes. This allows us to recommend researched toothpaste for application.

[Influence of Gentiana lutea L extract on blood coagulation].

The dry extract from the terrestrial parts of Gentiana Lutea was received in accordance to the developed by us general technological scheme. Study of the pharmacological influence of obtained extract on the coagulating properties of blood revealed that after its per os instillation into experimental animals the time of the formation of active thromboplastin reliably increases, while the time of thrombin and fibrinous cluster formation is shortened in comparison with those indices in the animals, that did not receive phyto-preparation, at the same time morphological appearance of the peripheral blood remains unchanged. Dry extract of terrestrial parts of Gentiana Lutea prepared in accordance to the technology recommended by us, together with widely known pharmacological effects, is characterized with new activity - influence on haemostasis. Obtained preliminary data concerning influence of the extract on coagulation of the blood request further deep studies of its mechanism. Revealed new activity of the terrestrial parts of Gentiana Lutea and the studies of the mechanism of its activity will serve in future as a basis for the recommendation of its use in new nosology. Terrestrial parts of Gentiana lutea L. are proposed as an alternative of the underground parts of the plant. Alongside with that, it is expedient to continue the studies devoted to the development of the haemostatic remedies of plant origin with systemic and local action (sponges, films, skin glues) from terrestrial parts of Gentiana lutea L.

[Creation of phytovaleologic drugs].

This is a new field of knowledge in medical sciences that means "Healthy Way of Life". Based on the data of medical plants and on the results of Phytochemical and Pharmacological studies, carried out in preliminary experiments, there are cultivated compositions of Valeological substances, potentially having radio protective activity. As the basis for the selection of raw materials and their correlation in mixture, there were taken the data of spontaneous analysis of chemical composition having pharmacological (radio protective) activity of various compositions. As the result, the types of studied raw materials and their parties by weight in total mixture have been defined: Valeological substances 1: 1. Tea (leaves); 2. Common plantain (leaves); 3. Horse gowan (flowers); 4. Blackberry (leaves) 5. Hips; 6. Peppermint (leaves). Valeological substances 2: 1. Tea (leaves); 2. Dandelion (leaves); 3. Horse gowan (flowers); 4. Knotgrass (herbage); 5. Hips; 6. Peppermint (leaves) Experimental studies revealed that the most optimal way of brewing tea is rational method. Valeological substances 1 and 2 (tea with radio protective activity) "Protector" and "Adjara" - correspondingly. Technical conditions and technological instructions for radio protective tea "Protector" and "Adjara" are created. Pharmacological studies of tea "Protector" and "Adjara" have taken place in the Branch of Biological University of Buryatia (Academy of Science of Russian Federation) under the supervision of Prof. S. M. Nikolaev. Radio protective teas "Protector" and "Adjara" is approved by the Ministry of Health Sciences.

[Radio protective drug production from fresh leaves of Aloe arborescens Mill].

Nowadays, phytogenous drugs are wildly used as radio protective substances. The aim of the research was to study radio protective characteristics of aloe juice fraction and to develop new technology for radio protective drug production. Technological scheme for getting the drug in two stages. The first stage - extraction of juice from fresh leaves; the second stage - extracting bagasse have been developed and optimal environment for bagasse extraction are defined: Infusion of bagasse with 96 % ethyl spirit (1:1) during 30 minutes, continuation of extracting with water on correlation to raw materials 10:1 at temperature of 70 degrees C during 30 minutes. For the basis of the first series of balanced loading there are taken the optimal parameters of extracting process, on the basis of which in its turn was developed technological scheme of getting dry extract of aloe. Dry extract is a fine-dispersed reddish-yellow (brownish-yellow) powder, which can be easily dissolved in warm (40-60 degrees C) water. Pharmacological researches were conducted in the Institute of General and Experimental Biology, Siberian Branch, Russian. Academy of Sciences. The remarkable radio protective effect of the drug was revealed.

[Hypolipidemic, anti-oxidant and anticoagulant action of camphor-25 compound].

Pharmacotherapeutic efficacy, anti-oxidant and anti-coagulant activity of camphor compound was tested in animals with experimental dyslipoproteinemia. Positive results of the experiment allows recommending this compound for prevention of an atherosclerotic damage of vascular endothelium and against thrombogenesis.

[Hepatoprotective effect of hepatophyt in experimental drug hepatitis].

The hepatoprotective action of Hepatophyt was studied on Wistar rats with experimental hepatitis caused by exposure of the animals to tetracycline. Hepatophyt was administered per os in a dose of 0.1 g/kg. Its significant pharmacotherapeutic action was due to the content of biologically active substances, mainly of polyphenol nature. As natural antioxidants, they provided stabilization of the biomembranes and increased the energetic potential of the hepatic cells and the contractile capacity of the pericanal microfilaments, thus stimulating the synthesis and transport of the cholates and excretion of bilirubin and cholesterol with bile.

[Phytocorrection of structural changes in the gastrointestinal tract caused by 6-mercaptopurine].

It was shown that the course use of Ventrofit, a complex plant remedy, normalized the structural and functional integrity of the stomach and thin intestine mucous membranes damaged by the use of 6-mercaptopurine.

[Effect of plant extracts from Baikal region on the choleretic reaction in white rats].

In the given work was estimated the choleretic activity of the extracts that was derived from the plants of the Baikal region. It had been shown that the experimental-therapeutic doses of the extracts stimulate choleretic reaction in intact rats.

[A neuroprotective effect of the herbal drug neurofit in alcoholic intoxication].

Our studies show that a combined herbal medicine neurofit has a pronounced neuroprotective effect in experimental alcoholic intoxication. The principal mechanism of this neuroprotective effect lies in neurofit ability to inhibit lipid peroxidation and stimulate the antioxidant system.

[High-performance liquid chromatographic method for the determination of dihydroquercetin in extracts of medicinal plants].

Determination of dihydroquercetin in extracts of medicinal plants by a high-performance liquid chromatography is presented. The method is simple, quick, highly reproducible and can be widely applied in phytopharmacology.

[Effect of nephrophyte on the course of the hepatorenal syndrome caused by a complex of antituberculous drugs].

A complex of antituberculous drugs causes the hepatorenal syndrome in albino rats. The course administration of the plant agent nephrophyte in an experimental therapeutic dose during tuberculostatic therapy has a positive effect on the course of the hepatorenal syndrome.

[Hepatoprotective effect of Tibetan remedy "Dig-Da-Shi-Tan"].

In experiments on albino rats with toxic hepatitis due to their exposure to tetracycline hydrochloride it was shown that a course of therapeutic and preventive administration of Dig-da-shi-tan decoction had a strong hepatoprotective effect. Antioxidative, membrane integrative, cholagogic and antiinflammatory activities of the remedy were revealed.