RESUMO
The severity of ischemic injury was evaluated by densitometry of brain samples stained with 2,3,5-triphenyltetrazolium chloride (TTC) on a rat model of cerebral ischemia/reperfusion (common carotid artery occlusion) and the neuroprotective activity of an extract of Astragalus membranaceus, Scutellaria baicalensis, and Phlojodicarpus sibiricus was assessed. Occlusion of the common carotid arteries led to a weakening of TTC staining of the brain tissue: densitometric indicators of the staining intensity for the cortex and striatum were lower than the corresponding indicators of sham-operated rats by 18.3 and 10.4%. The mean intensity of staining of brain samples did not differ in rats treated with the extract and sham-operated animals, which attested to its neuroprotective effect. The applied method is convenient for evaluation of the severity of ischemic brain damage at the early stages and screening potential neuroprotective agents.
Assuntos
Isquemia Encefálica , Fármacos Neuroprotetores , Extratos Vegetais , Animais , Ratos , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/uso terapêutico , Masculino , Isquemia Encefálica/tratamento farmacológico , Isquemia Encefálica/patologia , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Astragalus propinquus/química , Traumatismo por Reperfusão/tratamento farmacológico , Traumatismo por Reperfusão/patologia , Traumatismo por Reperfusão/prevenção & controle , Sais de Tetrazólio/química , Encéfalo/efeitos dos fármacos , Encéfalo/patologia , Ratos Wistar , Modelos Animais de Doenças , Scutellaria baicalensisRESUMO
Comparative data are presented for the choleretic activity of xanthones isolated from Gentianopsis barbata (Froel.) Ma. (Gentianaceae family): a sum of xanthone glycosides (I) and aglycons (II); xanthone aglycons, including decussatin [1-(OH)-3,7,8-(OCH3)3] (III) and gentiacaulein [1,7-(OH)-3,8-(OCH3)2] (IV); and xanthone glycoside gentiabavaroside [1-O-primverosyl-7-(OH)-3,8-(OCH3)2] (V). It was established that (II) is superior to (I) with respect to cholagogic effect and is inferior to (III) and (IV) with respect to cholatostimulant action.
Assuntos
Colagogos e Coleréticos/farmacologia , Gentianaceae , Xantenos/farmacologia , Animais , Bile/química , Bile/metabolismo , Colagogos e Coleréticos/química , Feminino , Masculino , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Ratos , Relação Estrutura-Atividade , Xantenos/químicaRESUMO
The granulated dry extract from Gentianopsis barbata (Froel) Ma in a dose of 0.1 g/kg produced a pronounced therapeutic effect in rats with experimental toxic (tetrachloromethane) and drug-induced (tetracycline) hepatitis. The gentian extract improved the bile production and secretion functions of liver, normalized the protein, lipid, and pigment metabolism, and increased the antioxidant system activity in the test animals.