Two new species and one new record of larvae of the family Johnstonianidae (Acari: Prostigmata) from Iran with a key to species of the genus Diplothrombium.

Diplothrombium sahragardi sp. nov. and Diplothrombium ostovani sp. nov. (Acari: Johnstonianidae) collected from soil samples (off host) in a forest near Asalem city (Iran) are described. Another species of this family Johnstoniana parva Wendt, Wohltmann, Eggers and Otto, 1994 is reported for the first time from Iran. A larva-based key to Diplothrombium is provided.