Antioxidant effects of nerolidol in mice hippocampus after open field test.

The aim of this study was to evaluate the neuroprotective effects of nerolidol in mice hippocampus against oxidative stress in neuronal cells compared to ascorbic acid (positive control) as well as evaluated the nerolidol sedative effects by open field test compared to diazepam (positive control). Thirty minutes prior to behavioral observation on open field test, mice were intraperitoneally treated with vehicle, nerolidol (25, 50 and 75 mg/kg), diazepam (1 mg/kg) or ascorbic acid (250 mg/kg). To clarify the action mechanism of of nerolidol on oxidative stress in animals subjected to the open field test, Western blot analysis of Mn-superoxide dismutase and catalase in mice hippocampus were performed. In nerolidol group, there was a significant decrease in lipid peroxidation and nitrite levels when compared to negative control (vehicle). However, a significant increase was observed in superoxide dismutase and catalase activities in this group when compared to the other groups. Vehicle, diazepam, ascorbic acid and nerolidol groups did not affected Mn-superoxide dismutase, catalase mRNA or protein levels. Our findings strongly support the hypothesis that oxidative stress occurs in hippocampus. Nerolidol showed sedative effects in animals subjected to the open field test. Oxidative process plays a crucial role on neuronal pathological consequence, and implies that antioxidant effects could be achieved using this sesquiterpene.

Avaliação do potencial antioxidante in vitro do nerolidol / Evaluation of antioxidant potential in-vitro of nerolidol

The antioxidant potential of nerolidol against the formation of thiobarbituric acid reactive substances (TBARS), hydroxyl radical and nitric oxide production was assessed in this study. At concentrations of 0.9, 1.8, 3.6, 5.4 and 7.2 mg/mL, it was observed that nerolidol prevented lipid peroxidation induced by 2,2?-azobis-2-amidinopropane (AAPH), inhibiting the formation of TBARS in a manner similar to that of Trolox, a synthetic hydrophilic ?-tocopherol analog that is widely used as an antioxidant standard. Nerolidol also reduced the production of the hydroxyl radical and nitric oxide, possibly indicating an in-vitro antioxidant property. This property could be exploited to protect in-vivo molecular targets (DNA, lipids, carbohydrates and proteins). Although nerolidol exhibited in-vitro antioxidant activity, by its capacity to remove hydroxyl radicals and nitric oxide and to prevent the formation of TBARS, more research is needed to characterize better the antioxidant potential of this compound.

O presente estudo avaliou o potencial antioxidante do nerolidol contra a formação de substâncias reativas ao ácido tiobarbitúrico (TBARS), radical hidroxila e produção de óxido nítrico. Nas concentrações de 0,9, 1,8, 3,6, 5,4 e 7,2 ?g/mL foi observado que o nerolidol preveniu a peroxidação lipídica induzida por 2,2?-azobis-2-amidinopropano(AAPH), inibindo a quantidade de TBARS formado, de maneira semelhante ao composto Trolox, um análogo sintético hidrofílico do ?-tocoferol, que é muito utilizado como padrão antioxidante. Além disso, o nerolidol também reduziu a produção do radical hidroxila, assim como a produção de óxido nítrico, possivelmente devido a uma propriedade antioxidante in vitro, que pode ser explorada para a proteção in vivo de alvos moleculares (DNA, lipídios, carboidratos e proteínas). O nerolidol apresentou potencial antioxidante in vitro, por meio da capacidade de remoção de radicais hidroxilas e do óxido nítrico, bem como na prevenção da formação de TBARS. No entanto, mais estudos são necessários para caracterizar melhor o potencial antioxidante in vitro deste composto