RESUMO
The study was aimed at tracking the proteomic profile of urine in 8 normal volunteers to 5-day dry immersion (DI). The proteome composition was determined by chromatography-mass spectrometry on high-efficient on-line liquid nano chromatograph Agilent 1100; complementary information about the protein spectra was obtained by dint of mass-spectrometer MaXis Impact 4G and hybrid mass-spectrometer LTQ-FT. Functional associations between proteins and biological functions were analyzed using computer system ANDCell (Associative Networks Discovery in Cells). A total of 256 proteins were identified; for 43 proteins difference in the detection rate during the baseline data collection and on DI day 4 exceeded 20%.
Assuntos
Adaptação Fisiológica , Proteinúria/metabolismo , Proteoma/análise , Simulação de Ausência de Peso , Adulto , Cromatografia Líquida de Alta Pressão , Humanos , Masculino , Espectrometria de Massas , Anotação de Sequência Molecular , Voo Espacial , Ausência de PesoRESUMO
The paper presents the results of the experiment with 7-day dry immersion. Eight healthy men were studied before, during and after exposure. It is shown that the primary response involves hemodynamic and water-electrolyte changes. The mechanisms of cardiovascular adaptation to immersion conditions are revealed. In particular, some electrophysiological shifts in propagation of myocardial excitation are found, leading to increase in "myocardium" index, estimated by analysis of natural small oscillations in the amplitude of the PQRST complex with dispersion ECG mapping. Revealed significant reduction of functional reserves of the cardiovascular regulatory mechanisms in the process of adaptation to dry immersion is of great practical importance. The results suggest that water-electrolyte shifts induced by dry immersion are the basis and the first phase of the further changes in autonomic regulation and myocardial functional state.