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Biosci Biotechnol Biochem ; 88(6): 679-688, 2024 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-38499443

RESUMO

Recently, it has been suggested that brown and beige adipocytes may ameliorate obesity because these adipocytes express uncoupling protein-1 (UCP-1), which generates heat by consuming lipid. However, obesity-induced inflammation suppresses the expression of UCP-1. To improve such conditions, food components with anti-inflammatory properties are attracting attention. In this study, we developed a modified system to evaluate only the indirect effects of anti-inflammatory food-derived compounds by optimizing the conventional experimental system using conditioned medium. We validated this new system using 6-shogaol and 6-gingerol, which have been reported to show the anti-inflammatory effects and to increase the basal expression of UCP-1 mRNA. In addition, we found that the acetone extract of Sarcodon aspratus, an edible mushroom, showed anti-inflammatory effects and rescued the inflammation-induced suppression of UCP-1 mRNA expression. These findings indicate that the system with conditioned medium is valuable for evaluation of food-derived compounds with anti-inflammatory effects on the inflammation-induced thermogenic adipocyte dysfunction.


Assuntos
Adipócitos , Anti-Inflamatórios , Inflamação , Macrófagos , RNA Mensageiro , Proteína Desacopladora 1 , Proteína Desacopladora 1/metabolismo , Proteína Desacopladora 1/genética , Animais , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Camundongos , Meios de Cultivo Condicionados/farmacologia , Anti-Inflamatórios/farmacologia , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Inflamação/genética , Adipócitos/efeitos dos fármacos , Adipócitos/metabolismo , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Linhagem Celular , Regulação da Expressão Gênica/efeitos dos fármacos
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