Ultrastructural changes in esophageal tissue undergoing stretch tests with possible impact on tissue engineering and long gap esophageal repairs performed under tension.

Esophageal biomechanical studies are being performed to understand structural changes resulting from stretches during repair of esophageal atresias as well as to obtain biomechanical values for tissue-engineered esophagus. The present study offers insights into ultrastructural changes after stretching of the ovine esophagus using uniaxial stretch tests. In vitro uniaxial stretching was performed on esophagi (n = 16) obtained from the abattoir within 4-6 h of 1-month-old lambs. Esophagi were divided into 4 groups (4 esophagi/group): control, Group1 (G1), Group2 (G2), Group3 (G3) stretched to 20%, 30% and 40% of their original length respectively. Force and lengthening were measured with 5 cycles performed on every specimen. Transmission electron microscopic (TEM) studies were performed on the 4 groups. During observational TEM study of the control group there were no significant differences in muscle cell structure or extracellular matrix. In all stretched groups varying degrees of alterations were identified. The degree of damage correlated linearly with the increasing level of stretch. Distance between the cells showed significant difference between the groups (control (µ = 0.41 µm, SD = 0.26), G1 (µ = 1.36 µm, SD = 1.21), G2 (µ = 2.8 µm, SD = 1.83), and G3 (µ = 3.01 µm, SD = 2.06). The diameter of the cells (control µ = 19.87 µm, SD = 3.81; G1 µ = 20.38 µm, SD = 4.45; G2 µ = 21.7 µm, SD = 6.58; G3 µ = 24.48 µm, SD = 6.69) and the distance between myofibrils (control µ = 0.23 µm, SD = 0.08; G1 µ = 0.27 µm, SD = 0.08; G2 µ = 0.4 µm, SD = 0.15; G3 µ = 0.61 µm, SD = 0.2) were significantly different as well ( p < 0.05 was considered to be significant). Esophageal stretching > 30% alters the regular intracellular and extracellular structure of the esophageal muscle and leads to disruption of intra- and extracellular bonds. These findings could provide valuable insights into alterations in the microscopic structure of the esophagus in esophageal atresias repaired under tension as well as the basis for mechanical characterization for tissue engineering of the esophagus.

Case reports of pediatric electrical finger burn injuries' management and late- onset complications / Gyermekkori, áramégés okozta kézujjsérülések ellátásáról és késoi szövodményeirol

Pediatric electrical injuries are rare; they only constitute 2-10% of all burn causes. Determination of their actual severity may be challenging due to their small entry and exit wounds. Deep necrosis develops during electrical burns in most cases. These injuries can damage the skin, soft and bone tissues, and in children, the growth plate, which may cause secondary deformities. The objective of these case reports was the presentation of paediatric electrical finger injuries' management and late-onset complications. A 15-year-old boy touched an electric wire while changing a lightbulb, which caused a burn injury on his right index finger. During the physical examination, a 25 x 14 mm, third-degree burn was identified volarly, above the distal interphalangeal joint as an entry wound, and an 8 x 7 mm exit site occurred dorsally at the nailbed's lateral edge. Necrectomy and cross finger flap surgery were performed. The cross flap was separated three weeks after the primary reconstruction. Throughout the follow-up examinations, the ulnar deviation of the distal digit was observed. X-ray confirmed the bone atrophy of the distal phalanx base. A 2-year old girl inserted a nail into the power outlet, causing third-degree burns on her thumb around the interphalangeal joint and hypothenar region. After necrectomy, the thumb's skin defect was reconstructed with a rotated flap, while the donor site received full-thickness skin graft transplantation. The follow-up of the child is still ongoing. Long term follow-up of these patients is necessary to identify and treat late-onset complications.

[An unusual cause of the hand cyst: finger dirofilariasis]. / Ujjon lévo cystosus elváltozás hátterében diagnosztizált dirofilariosis.

Dirofilariasis is a zoonosis affecting dogs and cats. It can be transmitted to human by mosquito bites. Because of the local inflammation caused by the parasite, a lump may develop. The authors present a case of a 13-year-old boy, who was diagnosed with dirofilariasis in association with a cyst located on the middle finger of the right hand. During physical examination, a lump on the extensor surface of the middle phalanx of the right middle finger was found. Soft tissue ultrasound was performed, which indicated the presence of a worm in the cyst. The cyst was surgically removed under local anesthesia and with parasitology test Dirofilaria repens infection was confirmed. The child had no complains or symptoms during recovery after the surgery. A solitary growth developing on the fingers of the hand often presents a differential diagnostic problem. Ultrasound can be greatly helpful in the preoperative diagnosis. Orv. Hetil., 2016, 157(39), 1571-1574.

Combination of vesicoureteric reflux and vesicoureteric junction obstruction.

OBJECTIVE: This study analysed the association of vesicoureteric reflux (VUR) and vesicoureteric junction obstruction (VUJO) requiring surgical interventions in infants and children. MATERIAL AND METHODS: Over 30 years (1975-2004) 423 infants and children were operated on because of VUR, 163 owing to VUJO and 25 patients (33 ureters) with a combination of VUR and obstruction of the vesicoureteric junction on the same side. For both pathological entities ureteral reimplantation was performed along with excision of the narrowed and refluxing distal ureteric segment. The age of patients at surgery ranged from 3 months to 11 years (average 2.6 years). The female to male ratio was 1.4:1. RESULTS: Out of the 25 patients (33 ureters), both entities were diagnosed before surgery in 10 of them (15 ureters). In 15 cases (18 ureters), only the VUR was preoperatively diagnosed; however, the VUJO was only suspected and confirmed during the operation. In one refluxing ureter, the obstruction was not diagnosed during subureteric endoscopic injection of the orifice. In five of the 33 ureters, redo reimplantation was necessary because of obstruction (four ureters) or reflux (one ureter). CONCLUSIONS: A combination of VUR and VUJO should be taken into consideration in a patient with proven reflux, where the ureter is dilated and tortuous and following urination the hydronephrosis and hydroureter persist or slowly decrease. In such cases long-term prophylaxis and endoscopic treatment are contraindicated but open surgery (reimplantation) is recommended.

Altered expression of c-kit-positive cells in the ureterovesical junction after surgically created vesicoureteral reflux.

PURPOSE: Peristaltic contractions propel urine unidirectionally from the renal pelvis trough the ureter and into the bladder. A morphologically and functionally competent ureterovesical junction prevents vesicoureteral reflux (VUR). According to current knowledge, pyeloureteral peristalsis is driven by atypical muscle cells within the upper urinary tract. Another likely relevant cell population, which is c-kit-positive, has been shown to be present within the whole urinary tract. Morphological changes of c-kit-positive cells have been described in VUR and pyeloureteral junction (PUJ) obstruction. Nevertheless, the functional importance of c-kit-positive interstitial cells has not yet been elucidated fully. Therefore, we investigated the influence of experimentally created VUR on the expression of c-kit-positive cells. MATERIALS AND METHODS: We created left-sided unilateral VUR in eight Vietnamese pigs using an open surgical technique. The VUR was shown to be grade II-III by voiding cystourethrogram (VCUG), 1 and 6 months after the procedure. The animals were killed after 6 months. The vesicoureteral junction and the distal ureters were excised and fixed in 4% PFA. Paraffin sections were stained using c-kit immunohistochemistry. A quantitative evaluation was performed by two independent investigators. The unaffected, contralateral, nonrefluxing ureters served as controls. RESULTS: We identified two types of c-kit-immunoreactive cells within the ureterovesical junction and distal ureter. The first group was comprised of round-shaped cells with substantial intracellular granulas, which resembled mast cells. Mast cells were found in the subepithelial region as well as between the muscle bundles. The number of mast cells was slightly increased in the VUR group. The second group consisted of spindle-shaped, bipolar ICC-like cells, which were seen mainly in the submucosal and muscular layers. The number of spindle-shaped c-kit-immunoreactive cells was markedly decreased in the refluxing ureter compared to the controls. CONCLUSIONS: Surgically created VUR leads to altered expression of c-kit-positive cells. The altered distribution of c-kit-positive ICC-like cells might further contribute to the impairment of coordinated pyeloureteral peristalsis.

Role of the Teflon deposit in the recurrence of vesicoureteral reflux.

A relationship between the Teflon deposit, visible with ultrasound, and long-term success of subureteric Teflon injection (STING) treatment was investigated. The study included only those patients with primary vesicoureteral reflux (VUR), in whom the reflux had disappeared and the Teflon deposits were visible 6 weeks following STING treatment. Cessation of VUR was proven by voiding cysto-urethrography (VCUG) in 99 patients (143 ureters). Average follow-up time was 9 (4-12) years. Patients were divided into two groups: group I, deposits visible with ultrasound [deposit (+)], and group II, no visible deposits at the end of the follow-up period [deposit (-)]. Reflux recurrence, the occurrence of urinary tract infection (UTI), and pyelonephritis were investigated, and technetium scintigraphy scans were examined. The deposit (+) group included 43 patients (65 ureters), and the deposit (-) group contained 56 patients (78 ureters). In the deposit (+) group there were no recurrences of VUR; however, 17 recurrences were found in the deposit (-) group (P < 0.05). Dimercaptosuccinic acid (DMSA) scintigraphy scans and occurrence of UTI showed significant difference between the groups (P < 0.05). A close relationship was found between the disappearance of the Teflon deposit and the recurrence of VUR. Disappearance of the Teflon deposit and repeated bacteriuria is a warning sign of the recurrence of VUR; therefore, VCUG might be warranted for these patients.

Posterior sagittal approach with perirectal dissection for reconstructive surgery of severe urogenital anomalies.

There are many approaches for surgery of complicated congenital and acquired urogenital anomalies in children with intact rectum. Pena advocates the mid-sagittal division of the sphincter mechanism posterior and anterior to the rectum, along with opening of the posterior and anterior rectal walls. The aim of this study is to determine whether the posterior sagittal approach with perirectal dissection (PSAPD) and elevation of the mobilized rectum would impair fecal continence when used for correction of complicated urogenital anomalies in children with normal rectum. Between 1988 and 1994 the authors performed PSAPD in eight infants and children with an intact anorectum. Indications for PSAPD were high vaginal atresia, Mullerian duct remnants, prostatic rhabdomyosarcoma, and traumatic vesicovaginal fistula. After a mean 10-year follow-up the bowel habits were assessed. Anorectal and uromanometric studies and a detailed questionnaire (modified Holschneider's scoring) sent to children or parents were evaluated. Three patients who preoperatively were clinically fecal continent had soiling only at the time of diarrhea. Early postoperative low anorectal pressure profile normalized during the follow-up. Seven patients had a fecal continence score above 23, two of them with maximum points of 26. Only one girl had a low score of 15. The authors conclude the PSAPD which offers a few advantages over the sagittal division of the rectum provides an alternative approach for selected lesions of the genitourinary tract in children with a normal rectum. Our results suggest that fecal continence is either preserved or partially affected.

Histologic findings after gastrocystoplasty in rabbits.

PURPOSE: The aim of this study was to investigate the long-term histologic changes after bladder augmentation with gastric segment in an animal subject. MATERIALS AND METHODS: Gastrocystoplasty was performed in 13 young, 3-month-old male rabbits. Open biopsies were taken from the native bladder and the gastric segment preoperatively and at 3, 6, and 12 months postoperatively. Sections were examined with H&E and periodic acid-Schiff (PAS) staining. Indirect immune peroxidase method was additionally applied to detect the carcinoembrionic antigen, the proliferative activity, and the gene for the tumor protein p53 in the epithelium. RESULTS: On the native bladder, at the 3-month follow-up, polyps, mucosal edema, submucosal fibrosis, and squamous cell metaplasia were detected, which did not change during the follow-up. On the gastric segment, at the 3-month follow-up, parietal cell hyperplasia and inflammatory mucosal overgrowth were detected; at the 6-month follow-up, inflammation or atrophy of the gastric mucosa and colonic-type metaplasia was found. These alterations remained unchanged during later course of follow-up. Neither dysplasia nor malignancy was observed during the 12-month follow-up. CONCLUSIONS: The present study supports the clinical observations of low cancer risk after gastrocystoplasty and may indicate different effect of gastric secretion on uroepithelium and that of urine on gastric mucosa.

[Late reconstructive surgery for cloaca malformations]. / Nem kielégítóen kezelt cloaca malformációk késói rekonstrukciós mútétei.

The rarity of cloacal anomalies, the wide range of anatomical variants, and the number of different operations mean that successful management of a patient (neonate or child) with this condition is one of the greatest challenges for paediatric surgeons. The authors describe the complex corrections of 3 patients with cloacal malformation (age 10-13 years). The cloacal malformations were not diagnosed at neonatal age and therefore only the anorectal agenesis was corrected at the first stage. The authors strongly recommend that all components of this anomaly (urethra, vagina and anorectum) should be managed in one step. Because of the rarity and complexity of cloacal malformations, the anomaly should be operated on only in specialised centres.