Unlocking the potential of ponds and pondscapes as nature-based solutions for climate resilience and beyond: Hundred evidences.

Unlocking the full potential of ponds (small water bodies) and pondscapes (network of ponds) as Nature-based Solutions (NbS) is critical pursuit for enhancing ecosystems and societal resilience to climate change and other societal challenges. Despite scattered initiatives for pond/pondscape creation, restoration and management-each considered here a distinct NbS-there is a significant knowledge gap in utilising ponds/pondscapes as effective NbS. We aimed to assess these NbS in terms of their objectives, outcomes, effectiveness, multifunctionality, delivery of potentially conflicting effects, and the implementation process while considering their Nature's Contributions to People (NCPs, i.e., benefits to society). We compiled data on 183 NbS actions implemented across 93 ponds/pondscapes from 24 countries, predominantly from Europe, via a questionnaire distributed to experienced stakeholders implementing NbS in ponds/pondscapes. One single pond/pondscape may imply more than one NbS action. Two-thirds were in rural areas, and one-third in urban settings. Our analysis revealed that Creation of habitat for biodiversity was a primary delivery objective (targeted NCP) in the implementation of most NbS in ponds/pondscapes, often also combined with other NCPs such as Learning and inspiration, Regulation of water quantity, and Physical and psychological experiences, showcasing their intended multifunctionality. Implemented NbS primarily focused on climate change adaptation (especially Regulation of hazards and extreme events, and water quantity) rather than mitigation, with less emphasis on measures like direct greenhouse gas emissions reduction or enhancing carbon sinks. The costs associated with pond's NbS varied significantly depending on factors such as project scope, objectives, location, socio-economic-cultural system, and specific implementation requirements. The creation of ponds/pondscapes often entailed the highest financial investment, much more than their restoration or their management. In conclusion, our study underscores the multifunctionality of ponds/pondscapes and provides insights about their significant potential as cost-effective NbS for enhancing ecosystem and societal resilience to climate change and biodiversity. It underscores the importance of further research to fully understand and measure the diverse range of NbS they offer, particularly in the context of climate change mitigation. Standardised measurements of the NCP provided by these NbS in ponds/pondscapes are essential for validating managers' claims and exploring their role in addressing climate change.

Socio-economic or environmental benefits from pondscapes? Deriving stakeholder preferences using analytic hierarchy process and compositional data analysis.

Ponds occupy a large share of standing water worldwide and play an important role in providing various ecosystem services. There are concerted efforts of the European Union either to create new ponds, or to restore and preserve existing ponds as nature-based solutions to provide benefits to ecosystem and human well-being. As part of the EU PONDERFUL project, selected pondscapes (i.e. landscapes of ponds) in eight different countries - hereafter "demo-sites", are studied to comprehensively understand their characteristics and their efficiency to provide ecosystem services. In addition, the needs and knowledge of stakeholders who own, work, research, or benefit from the pondscapes are also important, because of their capabilities to create, manage and develop the pondscapes. Therefore, we established connection with stakeholders to study their preferences and visions on the pondscapes. Using the analytic hierarchy process, this study shows that in general stakeholders in the European and Turkish demo-sites prefer environmental benefits to economic benefits, while stakeholders in the Uruguayan demo-sites rank the economic benefits higher. More specifically, in the European and Turkish demo-sites, the biodiversity benefits, i.e. life-cycle maintenance, habitat and gene pool protection, receive the highest ranking among all groups. On the other hand, stakeholders at the Uruguayan demo-sites rank provisioning benefits as the most important, because many ponds in Uruguayan demo-sites are being used for agricultural purposes. Understanding those preferences helps policy makers to address the needs of stakeholders more correctly, when considering any action or policy for the pondscapes.

Ornamental ponds as Nature-based Solutions to implement in cities.

Small waterbodies such as ponds are widely represented in cities, contributing to the blue-green infrastructure, improving human well-being. Ornamental ponds are particularly abundant in the densest urbanized areas, especially in parks, in private grounds such as gardens and also imbedded in the green infrastructure. However, their multifunctionality remains infrequent, as generally aesthetic enjoyment is the main ecosystem service targeted. The promotion of native biodiversity is rarely a priority, as are other ecosystem services (e.g. flood mitigation or water purification). It is nevertheless questionable if such mono-functional ponds could also be able to provide other services. Indeed, an innovative approach would be to increase the multifunctionality of ornamental ponds, especially for biodiversity. This was investigated in 41 ornamental ponds designed for providing aesthetic enjoyment in the city of Geneva (Switzerland). The biodiversity was assessed, as well as selected ecosystem services (water retention, phytopurification, cooling effect, carbon sequestration). A survey among the population was also conducted. This survey underlined a recognized contribution of ornamental ponds to well-being. However, the assessment of the ecosystem services evidenced a lack of multifunctionality for most of these ponds. They presented a low biodiversity, compared to more natural ponds and to unimpaired ponds. Furthermore, they performed poorly for most other ecosystem services investigated. There were nevertheless exceptions, with selected ponds displaying a multifunctionality, even for ecosystem services for which they were not designed. It was also shown that ornamental ponds could easily be optimized for biodiversity by simple low-cost management measures. Additional ecosystem services could also be promoted. The performance of small ornamental ponds is best when ponds are considered collectively, as pondscapes, with their cumulative benefits. New ornamental pond implementation is therefore encouraged, as their multifunctionality turns them into Nature-based Solutions able to contribute to solving several societal challenges and to improve human well-being.

Perils of life on the edge: Climatic threats to global diversity patterns of wetland macroinvertebrates.

Climate change is rapidly driving global biodiversity declines. How wetland macroinvertebrate assemblages are responding is unclear, a concern given their vital function in these ecosystems. Using a data set from 769 minimally impacted depressional wetlands across the globe (467 temporary and 302 permanent), we evaluated how temperature and precipitation (average, range, variability) affects the richness and beta diversity of 144 macroinvertebrate families. To test the effects of climatic predictors on macroinvertebrate diversity, we fitted generalized additive mixed-effects models (GAMM) for family richness and generalized dissimilarity models (GDMs) for total beta diversity. We found non-linear relationships between family richness, beta diversity, and climate. Maximum temperature was the main climatic driver of wetland macroinvertebrate richness and beta diversity, but precipitation seasonality was also important. Assemblage responses to climatic variables also depended on wetland water permanency. Permanent wetlands from warmer regions had higher family richness than temporary wetlands. Interestingly, wetlands in cooler and dry-warm regions had the lowest taxonomic richness, but both kinds of wetlands supported unique assemblages. Our study suggests that climate change will have multiple effects on wetlands and their macroinvertebrate diversity, mostly via increases in maximum temperature, but also through changes in patterns of precipitation. The most vulnerable wetlands to climate change are likely those located in warm-dry regions, where entire macroinvertebrate assemblages would be extirpated. Montane and high-latitude wetlands (i.e., cooler regions) are also vulnerable to climate change, but we do not expect entire extirpations at the family level.

Contribution of artificial waterbodies to biodiversity: A glass half empty or half full?

Artificial ponds are increasingly created for the services they provide to humans. While they have the potential to offer habitats for freshwater biodiversity, their contribution to regional diversity has hardly been quantified. In this study, we assess the relative contribution of five types of artificial ponds to regional biodiversity of five different regions, studying amphibians, water beetles and freshwater snails. This biodiversity is also compared with that observed in natural ponds from three of the investigated regions. Our results indicate that artificial ponds host, on average, about 50% of the regional pool of lentic species. When compared to natural ponds, the artificial ponds always supported a substantially lower alpha richness (54% of the natural pond richness). The invertebrate communities presented high values of beta diversity and were represented by a restricted set of widely distributed species, and by numerous rare species. There were discrepancies among the taxonomic groups: overall, amphibians benefited most from the presence of artificial ponds, since 65% of the regional lentic species pools for this group was found in artificial ponds, whereas 43% and 42% was observed in the case of beetles and snails, respectively. However, each invertebrate group was promptly the most benefited animal group in a single pond type. Therefore, artificial pond types were complementary among them in terms of contribution to regional diversity of the three animal groups. Based on these results, we forecast that future human-dominated landscapes in which most ponds are artificial will be particularly impoverished in terms of freshwater biodiversity, underlining the need to conserve existing natural ponds and to create new "near-natural" ponds. However, if properly designed and managed, artificial ponds could make a substantial contribution to support freshwater biodiversity at a regional scale. Furthermore, the number and diversity of artificial ponds must be high in each considered landscape.

Effectiveness of amphibians as biodiversity surrogates in pond conservation.

Amphibian decline has led to worldwide conservation efforts, including the identification and designation of sites for their protection. These sites could also play an important role in the conservation of other freshwater taxa. In 89 ponds in Switzerland, we assessed the effectiveness of amphibians as a surrogate for 4 taxonomic groups that occur in the same freshwater ecosystems as amphibians: dragonflies, aquatic beetles, aquatic gastropods, and aquatic plants. The ponds were all of high value for amphibian conservation. Cross-taxon correlations were tested for species richness and conservation value, and Mantel tests were used to investigate community congruence. Species richness, conservation value, and community composition of amphibians were weakly congruent with these measures for the other taxonomic groups. Paired comparisons for the 5 groups considered showed that for each metric, amphibians had the lowest degree of congruence. Our results imply that site designation for amphibian conservation will not necessarily provide protection for freshwater biodiversity as a whole. To provide adequate protection for freshwater species, we recommend other taxonomic groups be considered in addition to amphibians in the prioritization and site designation process.

C-terminal sequences in R-Ras are involved in integrin regulation and in plasma membrane microdomain distribution.

The small GTPases R-Ras and H-Ras are highly homologous proteins with contrasting biological properties, for example, they differentially modulate integrin affinity: H-Ras suppresses integrin activation in fibroblasts whereas R-Ras can reverse this effect of H-Ras. To gain insight into the sequences directing this divergent phenotype, we investigated a panel of H-Ras/R-Ras chimeras and found that sequences in the R-Ras hypervariable C-terminal region including amino acids 175-203 are required for the R-Ras ability to increase integrin activation in CHO cells; however, the proline-rich site in this region, previously reported to bind the adaptor protein Nck, was not essential for this effect. In addition, we found that the GTPase TC21 behaved similarly to R-Ras. Because the C-termini of Ras proteins can control their subcellular localization, we compared the localization of H-Ras and R-Ras. In contrast to H-Ras, which migrates out of lipid rafts upon activation, we found that activated R-Ras remained localized to lipid rafts. However, functionally distinct H-Ras/R-Ras chimeras containing different C-terminal R-Ras segments localized to lipid rafts irrespective of their integrin phenotype.

Suppression of integrin activation by activated Ras or Raf does not correlate with bulk activation of ERK MAP kinase.

The rapid modulation of ligand-binding affinity ("activation") is a central property of the integrin family of cell adhesion receptors. The Ras family of small GTP-binding proteins and their downstream effectors are key players in regulating integrin activation. H-Ras can suppress integrin activation in fibroblasts via its downstream effector kinase, Raf-1. In contrast, to H-Ras, a closely related small GTP-binding protein R-Ras has the opposite activity, and promotes integrin activation. To gain insight into the regulation of integrin activation by Ras GTPases, we created a series of H-Ras/R-Ras chimeras. We found that a 35-amino acid stretch of H-Ras was required for full suppressive activity. Furthermore, the suppressive chimeras were weak activators of the ERK1/2 MAP kinase pathway, suggesting that the suppression of integrin activation may be independent of the activation of the bulk of ERK MAP kinase. Additional data demonstrating that the ability of H-Ras or Raf-1 to suppress integrin activation was unaffected by inhibition of bulk ERK1/2 MAP kinase activation supported this hypothesis. Thus, the suppression of integrin activation is a Raf kinase induced regulatory event that can be mediated independently of bulk activation of the ERK MAP-kinase pathway.