RESUMO
The dose dependence of natural killer (NK) cell activity from mouse spleen upon action of low-intensity millimeter waves in the exposure range from 5 to 96 hours was studied. It has found an increase of NK activity by 24 hours posttreatment that returned to normal level in a day after the cessation of the irradiation. Also the stimulation of isolated NK cell activity after millimeter waves treatment within 1 hour was revealed.
Assuntos
Campos Eletromagnéticos/efeitos adversos , Células Matadoras Naturais/efeitos da radiação , Baço/efeitos da radiação , Animais , Técnicas Citológicas , Citotoxicidade Imunológica/efeitos da radiação , Interpretação Estatística de Dados , Relação Dose-Resposta à Radiação , Técnicas In Vitro , Células Matadoras Naturais/citologia , Células Matadoras Naturais/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Doses de Radiação , Baço/citologia , Baço/imunologia , Fatores de TempoRESUMO
It was found that single total-body exposure to electromagnetic centimeter waves (8.15-18 GHz, 1 microW/cm2, 5 h) stimulated the proliferation of mouse T and B splenic lymphocytes. The same effects were observed upon in vivo treatment of rats for 5 h with millimeter waves (42.2 GHz, amplitude modulation 10 Hz, 1 microW/cm2). The whole-body irradiation with centimeter or millimeter waves did not cause any significant changes in natural activity of killer cells. The cellular responses induced by the irradiation of isolated animal cells in vitro did not coincide with those revealed after the total-body irradiation of animals. Thus, the in vitro irradiation of natural killer cells to millimeter waves for 1 h increased their cytotoxic activity whereas, after treatment to centimeter waves for the same time, the activity of killer cells did not change. On the contrary, irradiation of T and B lymphocytes with millimeter waves (42.2 GHz, amplitude modulation 10 Hz, 1 microW/cm2, 1 h) suppressed the blasttransformation of cells. The results show a higher immunostimulative potential of centimeter waves as compared to millimeter waves.
Assuntos
Linfócitos B/efeitos da radiação , Divisão Celular/efeitos da radiação , Sobrevivência Celular/efeitos da radiação , Campos Eletromagnéticos , Músculo Esquelético/efeitos da radiação , Linfócitos T/efeitos da radiação , Animais , Masculino , Camundongos , Músculo Esquelético/citologiaAssuntos
Sistema Imunitário , Bulbo Olfatório/imunologia , Bulbo Olfatório/fisiologia , Animais , Linfócitos B/metabolismo , Divisão Celular , Interleucina-2/biossíntese , Interleucina-3/biossíntese , Macrófagos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos , Óxido Nítrico/metabolismo , Bulbo Olfatório/cirurgia , Baço/citologia , Linfócitos T/metabolismo , Fatores de Tempo , Fator de Necrose Tumoral alfa/metabolismoRESUMO
The effect of low-level millimeter fractionated radiation on the production of tumor necrosis factor, intreleukin-2, interleukine-3, and nitric oxide and on the activity of natural killer cells and proliferation of T and B lymphocytes in mice was studied. Cell activity was measured in four groups of male Balb/c mice (control, exposed, tumor-bearing unexposed, and exposed tumor-bearing animals) within 30 days of tumoral growth and microwave exposure (42.2 GHz, 10 Hz amplitude modulation, 0.5 microW/cm2, 1.5 h daily). A significant increase in the production of tumor necrosis factor and nitric oxide and in the activity of natural killer cells was observed at the early stage of tumor development; this effect was considered as adaptive response. In healthy mice, millimeter radiation produced both stimulating and immunodepressive effects. The changes were nonmonotonous; as the exposure duration was increased, the stimulating effect became weaker and on day 30 it was not observed. Irradiation of tumor-bearing mice did not induce any significant changes in the activity of cells compared to unirradiated tumor-bearing animals. Moreover, exposure to millimeter waves impaired some characteristics of cell immunity in tumor-bearing mice. It was concluded that low-intensity millimeter waves do not increase the resistance against tumor as it was shown earlier in our experiments with centimeter waves.
Assuntos
Carcinoma de Ehrlich/imunologia , Campos Eletromagnéticos , Animais , Linfócitos B/patologia , Linfócitos B/efeitos da radiação , Carcinoma de Ehrlich/patologia , Divisão Celular/efeitos da radiação , Imunidade Celular/efeitos da radiação , Interleucina-2/biossíntese , Interleucina-3/biossíntese , Células Matadoras Naturais/efeitos da radiação , Ativação Linfocitária , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Transplante de Neoplasias , Óxido Nítrico/biossíntese , Baço/patologia , Baço/efeitos da radiação , Linfócitos T/patologia , Linfócitos T/efeitos da radiação , Fatores de Tempo , Fator de Necrose Tumoral alfa/biossínteseRESUMO
Production of tumor necrosis factor (TNF) has been investigated in peritoneal macrophages and splenic T cells of Arctic Yakutian ground squirrel (Citellus Undulatus Pallas) upon in vitro action of electromagnetic and ionizing radiation during annual cycle. The significant activation of TNF production in the cells of awaken ground squirrels in winter and increasing level of the lymphokine production at spring-summer period has been indicated. The level of TNF production in splenic T cells was not changed during whole year. The electromagnetic radiation (EMR) of low intensity (8.15-18 GHz, 1 microW/cm2) induced an augmentation of both secretory and proliferative activity in TNF-producing cells. Ionizing radiation suppressed T cell proliferation, but the doses 2 and 5 Gy resulted in a significant stimulation of TNF production in T cells and macrophages.
Assuntos
Fenômenos Eletromagnéticos , Hibernação/fisiologia , Macrófagos Peritoneais/efeitos da radiação , Radiação Ionizante , Sciuridae/fisiologia , Linfócitos T/efeitos da radiação , Fator de Necrose Tumoral alfa/biossíntese , Animais , Células Cultivadas/efeitos da radiação , Interpretação Estatística de Dados , Feminino , Masculino , Doses de Radiação , Estações do Ano , Baço/efeitos da radiação , Fatores de TempoRESUMO
The effect of prolonged treatment with weak microwaves on the production of tumor necrosis factor and interleukin-3 in peritoneal macrophages and T cells of male NMRI mice twice immunized by affinity-purified carboanhydrase was studied. Against the back ground of a high titer of antibody production, a significant increase in the production of tumor necrosis factor in peritoneal macrophages and splenic T lymphocytes of immunized mice was revealed, and a much stronger effect was observed for irradiated immunized animals. A tendency to increased secretion of interleukin-3 for unirradiated and irradiated immunized animals was found; in the latter group of animals, the effect being more pronounced. The stimulation of production of the cytokins, especially tumor necrosis factor, by combination of antigenic stimulation and microwaves can be used in adjuvant therapy of various immune diseases.
Assuntos
Interleucina-3/biossíntese , Macrófagos Peritoneais/efeitos da radiação , Micro-Ondas , Fator de Necrose Tumoral alfa/biossíntese , Animais , Macrófagos Peritoneais/metabolismo , Masculino , CamundongosRESUMO
The effect of bidistilled modified water obtained by treating bidistilled water with static electric field in the cathode compartment of a diaphragm electrolyzer on the production of tumor necrosis factor and activity of natural killer cells of mice was studied. It was found that bidistilled modified water added to cultured murine macrophages increases the tumor necrosis factor production 2.5-3-fold. A similar degree of stimulation of tumor necrosis factor production was observed after peroral administration of bidistilled modified water for 10-25 days. The activity of natural killer cells under these conditions increased by 150%. The stimulation of these immunity system components responsible for the resistance of the organism to tumors led to a suppression of tumor growth in tumor-bearing mice receiving bidistilled modified water.
Assuntos
Adjuvantes Imunológicos/uso terapêutico , Carcinoma de Ehrlich/tratamento farmacológico , Fator de Necrose Tumoral alfa/biossíntese , Água , Adjuvantes Imunológicos/química , Animais , Carcinoma de Ehrlich/imunologia , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Eletrólise , Humanos , Células Matadoras Naturais/efeitos dos fármacos , Células L , Macrófagos Peritoneais/metabolismo , Masculino , Camundongos , Baço/efeitos dos fármacos , Baço/patologia , Água/químicaRESUMO
The effect of low-intensity microwaves (8.15-18 GHz, 0.3 or 1 microW/cm2, 1.5 h daily for 30 days) on antibody production in healthy male NMRI mice after immunization with affinity-purified carboanhydrase isolated from bovine erythrocytes with and without Freund's adjuvant was studied. It was found that exposure to microwaves leads to an increase in the concentration of antibodies in blood plasma, the stimulating effect being more pronounced in the primary immune response. It is assumed that the effect of enhancement of the immune response by the action of centimeter microwaves can be used in the adjuvant therapy.
Assuntos
Formação de Anticorpos/efeitos da radiação , Micro-Ondas , Animais , Anidrases Carbônicas/imunologia , Bovinos , Adjuvante de Freund/imunologia , Masculino , Camundongos , VacinaçãoRESUMO
The effect of fractionated exposure to low-intensity microwaves (8.15-18 GHz, 1 microW/cm2, 1.5 h daily for 7 days) and combined weak magnetic field (constant 65 1 microT; alternating--100 nT, 3-10 Hz) on the production of tumor necrosis factor in macrophages of mice with experimental solid tumors produced by transplantation of Ehrlich ascites carcinoma was studied. It was found that exposure of mice to both microwaves and magnetic field enhanced the adaptive response of the organism to the onset of tumor growth: the production of tumor necrosis factor in peritoneal macrophages of tumor-bearing mice was higher than in unexposed mice.