Bio-electrochemical degradation of carbamazepine (CBZ): A comprehensive study on effectiveness, degradation pathway, and toxicological assessment.

Recent attention on the detrimental effects of pharmaceutically active compounds (PhACs) in natural water has spurred researchers to develop advanced wastewater treatment methods. Carbamazepine (CBZ), a widely recognized anticonvulsant, has often been a primary focus in numerous studies due to its prevalence and resistance to breaking down. This study aims to explore the effectiveness of a bio-electrochemical system in breaking down CBZ in polluted water and to assess the potential harmful effects of the treated wastewater. The results revealed bio-electro degradation process demonstrated a collaborative effect, achieving the highest CBZ degradation compared to electrodegradation and biodegradation techniques. Notably, a maximum CBZ degradation efficiency of 92.01% was attained using the bio-electrochemical system under specific conditions: Initial CBZ concentration of 60 mg/L, pH level at 7, 0.5% (v/v) inoculum dose, and an applied potential of 10 mV. The degradation pathway established by identifying intermediate products via High-Performance Liquid Chromatography-Mass Spectrometry, revealed the complete breakdown of CBZ without any toxic intermediates or end products. This finding was further validated through in vitro and in vivo toxicity assays, confirming the absence of harmful remnants after the degradation process.

Unleashing the room temperature boronization: Blooming of Ni-ZIF nanobuds for efficient photo/electro catalysis of water.

Water splitting provides an environmental-friendly and sustainable approach for generating hydrogen fuel. The inherent energetic barrier in two-core half reactions such as the Hydrogen Evolution Reaction (HER) and Oxygen Evolution Reaction (OER) leads to undesired increased overpotential and constrained reaction kinetics. These challenges pose significant challenges that demand innovative solutions to overcome. One of the efficient ways to address this issue is tailoring the morphology and crystal structure of metal-organic frameworks (MOF). Nickel Zeolite Imidazolate Framework (Ni-ZIF) is a popular MOF and it can be tailored using facile chemical methods to unleash a remarkable bifunctional electro/photo catalyst. This innovative solution holds the capability to address prevailing obstacles such as inadequate electrical conductivity and limited access to active metal centers due to the influence of organic ligands. Thereby, applying boronization to the Ni-ZIF under different duration, one can induce blooming of nanobuds under room temperature and modify oxygen vacancies in order to achieve higher reaction kinetics in electro/photo catalysis. It can be evidenced by the 24-h boronized Ni-ZIF (BNZ), exhibiting lower overpotentials as electrocatalyst (OER-396 mV & HER-174 mV @ 20 mA/cm2) in 1 M KOH electrolyte and augmented gas evolution rates when employed as a photocatalyst (Hydrogen-14.37 µmol g-1min-1 & Oxygen-7.40 µmol g-1min-1). The 24-h boronization is identified as the optimum stage of crystalline to amorphous transformation which provided crystalline/amorphous boundaries as portrayed by X-Ray diffraction (XRD) and High Resolution-Transmission Electron Microscopy (HR-TEM) analysis. The flower-like transformation of 24-BNZ, characterized by crystalline-amorphous boundaries initiates with partial disruption of Ni-N bonds and formation of Ni-B bonds as evident from X-ray Photoelectron Spectroscopy (XPS). Further, the 24-h BNZ exhibit bifunctional catalytic activities with pre-longed stability. Overall, this work presents a comprehensive study of the electrocatalytic and photocatalytic water splitting properties of the tailored Ni-ZIF material.

Evaluation of Lentilactobacillus parafarraginis A6-2 strain for aluminum removal and anti-inflammatory effects: implications for alleviating Al toxicity.

AIM: To assess the effectiveness of Lentilactobacillus parafarraginis A6-2 cell lysate for the removal of aluminum (Al), which induces neurotoxicity, and its protective effect at cellular level. METHODS AND RESULTS: The cell lysate of the selected L. parafarraginis A6-2 strain demonstrated superior Al removal compared to live or dead cells. The Al removal efficiency of L. parafarraginis A6-2 cell lysate increased with decreasing pH and increasing temperature, primarily through adsorption onto peptidoglycan. Neurotoxicity mitigation potential of L. parafarraginis A6-2 was evaluated using C6 glioma cells. C6 cells exposed with increasing concentration of Al led to elevated toxicity and inflammation, which were gradually alleviated upon treatment with L. parafarraginis A6-2. Moreover, Al-induced oxidative stress in C6 cells showed a concentration-dependent reduction upon treatment with L. parafarraginis A6-2. CONCLUSIONS: This study demonstrated that L. parafarraginis A6-2 strain, particularly in its lysate form, exhibited enhanced capability for Al removal. Furthermore, it effectively mitigated Al-induced toxicity, inflammation, and oxidative stress.

Lactiplantibacillus sp. D10-2: potential bacteria for eliminating bisphenol A and reducing BpA-induced lipid accumulation.

Bisphenol A (BpA) is an endocrine-disrupting substance commonly found in plastics and resins. It is reported that BpA exposure induces lipid accumulation in humans, similar to obesogenic compounds. The main objective of this study is to investigate the removal of BpA using Lactiplantibacillus sp. D10-2, and to examine its potential for reducing BpA-induced lipid accumulation in 3T3-L1 cell line model. The heat-dried cells of Lactiplantibacillus sp. D10-2 showed 69.7% removal efficiency for initial BpA concentration of 10 µg/mL, which was 30.5% higher than the live cells. The absence of metabolites or intermediates in BpA removal studies indicates that the Lactiplantibacillus sp. D10-2 strain removed BpA by adsorption process. The hydrophobic interactions of heat-dried Lactiplantibacillus sp. D10-2 cells were observed to be higher with 33.7% compared to live cells (15.0%), suggesting a stronger ability to bind with BpA. Although the BpA binding onto Lactiplantibacillus sp. D10-2 was not affected by pH, it was confirmed that as the temperature increases, the binding ability got decreased due to mass transfer and diffusion of BpA molecules. Treatment with Lactiplantibacillus sp. D10-2 (0.1, 0.25, 0.5, 1%) reduced lipid accumulation by 61.7, 58.0, 52.7 and 60.4% in 3T3-L1 cells exposed with BpA. In addition, it was confirmed that Lactiplantibacillus sp. D10-2 treatment suppressed the protein expression levels of lipogenesis-related PPARγ and C/EBPα in 3T3-L1 cells. The results of the study suggest that the Lactiplantibacillus sp. D10-2 strain can remove BpA and reduce BpA-accelerated lipid accumulation in 3T3-L1 cells.

In situ fabrication of Ag decorated porous ZnO photocatalyst via inorganic-organic hybrid transformation for degradation of organic pollutant and bacterial inactivation.

In this study, in situ silver (Ag) - porous ZnO photocatalysts were synthesized via solvothermal and post-annealing treatment. The formation of the porous ZnO structure due to the removal of organic moieties from the inorganic-organic hybrids Ag-ZnS(en)0.5 during the annealing process. The optimal Ag-ZnO photocatalyst showed excellent photocatalytic degradation activity, with 95.5% orange II dye and 97.2% bisphenol A (BPA) degradation under visible light conditions. Additionally, the photocatalytic inactivation of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) led to a 97% inactivation rate after 2 h under dark conditions. Trapping experiments suggest that the superoxide anion (O2-) radicals are the main active species to degrade the organic dye. The improved photocatalytic dye degradation activity and inactivation of bacteria were attributed to the synergistic effect of Ag and porous ZnO structure, increased surface area, and efficiently separated the photoexcited charge carriers. This work could provide an effective strategy for the synthesis of porous structures toward organic pollutant degradation and bacterial inactivation in wastewater.

Applied potential assisted biodegradation of amoxicillin (AMX) using bacterial consortium isolated from a waste dump site.

Antibiotics have revolutionized modern day living with their ability to effectively treat infectious diseases in humans and animals. However, the release of antibiotic compounds into the environment has led to toxic consequences. To reduce this environmental impact, it is important to employ an inexpensive and rational technology to reduce the amount of antibiotics released into the ecosystem. This study aims to explore the potential of using a bio-electrochemical system (BES) to remove Amoxicillin (AMX) from artificially contaminated soil using a microbial consortium and pure culture isolates. Under desired conditions, including an initial AMX concentration of 150 mg/L, 5 mg/L tryptone as the nitrogen source, pH of 7, temperature of 29 °C, an applied potential of 0.8 V, and an inoculum dose of 1% w/v, the BES showed a maximum degradation of 97.9% of AMX with the microbial consortium (HP03, HP09, and HP10). High performance liquid chromatography-mass spectrometry was used to analyse the intermediates formed during the degradation process, and the pathway elucidated revealed complete degradation of AMX. Phytotoxicity studies and degradation efficiency against multiple antibiotics confirmed the environmental significance of the BES with microbial consortium. Overall, this study highlights the potential of BES as a cost-effective and efficient method for reducing the release of antibiotics into the environment and provides valuable insights into the mechanisms and pathways of antibiotic degradation.

Detoxification of p-nitrophenol (PNP) using Enterococcus gallinarum JT-02 isolated from animal farm waste sludge.

Enterococcus gallinarum (JT-02) isolated and identified from the animal farm waste sludge was found to be capable of biodegrading p-nitrophenol (PNP), an organic compound used to manufacture drugs, fungicides, insecticides, dyes, and to darken leather. The intention of this study was to optimize the biodegradation by finding the optimal conditions for the specific strain through single-factor experiments. The bacterial strain was grown in Luria Bertani broth and various parameters were optimized to achieve the prime settings for the p-nitrophenol (PNP) biodegradation. The results indicated that the best setups for the biodegradation by the strain JT-02 was 100 mg/L of PNP; pH 7; 30 °C; 150 rpm in a shaker incubator and 3% (v/v) of inoculum dose. Once the optimal conditions were found, the bacteria were capable of degrading p-nitrophenol (98.21%) in 4 days. Intermediates produced during PNP biodegradation were identified using High Performance Liquid Chromatography (HPLC) analysis and the biodegradation pathway was elucidated. Phytotoxicity studies were carried out with Vigna radiata seeds to confirm the applicability and efficiency of PNP biodegradation.

Effect of fermentation on antioxidant, antimicrobial, anti-inflammatory, and anti-Helicobacter pylori adhesion activity of Ulmus davidiana var. japonica root bark.

The limited yield of Ulmus davidiana var. japonica root bark (URB) extract is considered an economic loss to the food industry. Improving extraction yield and bioactivity through fermentation increase the industrial usage of URB. The study aims to optimize the fermentation with cellulolytic and pectinolytic bacteria and evaluate the bioactivity and anti-Helicobacter pylori activity of the fermented URB extract. URB fermentation with the Bacillus licheniformis FLa3, isolated from salted seafood (Sardinella zunasi), under optimal conditions (37 °C, pH 6, 10% inoculum dose, and 36 h) improved the extraction yield by 36% compared to the control. The antioxidant and antimicrobial activity of the fermented extract were significantly higher than non-fermented extract. High-performance liquid chromatography results confirmed that the fermentation increased the proportion of bioactive components such as catechin (171.7%), epicatechin (144.3%), quercetin (27.3%), and kaempferol (16.7%). The results confirmed that the fermentation increased both the extraction yield and bioactivity.

Cold plasma-assisted regeneration of biochar for dye adsorption.

Environmental remedies, including adsorption-based water purification, are now being asked to meet the requirement for a low-carbon circular economy requiring low energy and low material consumption. In this regard, we tested the possibility of regenerating adsorbents via cold plasma (CP) treatment for less use of adsorbents and no washing solution. In the adsorption of methylene blue (MB) using carbonized rice husk (CRH) and five successive regeneration cycles by CP treatment, the removal efficiencies were maintained at a moderate level (∼70% of the initial performance), unlike five consecutive adsorption without CP treatment (∼9-13% of the initial performance). The regeneration of CRH by CP treatment was also double-checked by the FESEM, EDS, BET, FTIR, XPS, and surface zeta potential measurements. The successfully recovered adsorption capability is related to the remediation of adsorption sites. It is also worth noting that the required power consumption for recycling by CP treatment was about 6.4 times lower than carbonizing new rice husks. This work provides insights into recovering adsorbents using CP without rigorous, costly, and energy-intensive processes.

Improved visible-light-driven photocatalytic removal of Bisphenol A using V2O5/WO3 decorated over Zeolite: Degradation mechanism and toxicity.

WO3/Zeolite/V2O5 (TZV) composite synthesized through co-precipitation was used for the degradation of Bisphenol-A (BpA). XRD and Raman spectra were employed to ascertain the crystallinity of the composite. The pristine nature of the compound without any free particles over the zeolite surface was established through FESEM, thus, substantiating the composite character of the material. The enhancement in activity after doping with WO3 was ascertained by DRS-UV. Photocatalytic degradation studies clearly established the superiority of TZV 10 over bare V2O5. Complete BpA degradation (100%) was attained at 50 min of incubation with 0.75 g/L TZV-10 in acidic medium (pH 3) for an initial BpA concentration of 100 mg/L. HPLC-MS/MS analysis was used to decipher the degradation pathway. The catalyst was stable even after 9 cycles. Phytotoxicity studies and lake water treatment results proved the environmental efficiency of the synthesized material.

Significance of LED lights in enhancing the production of vinegar using Acetobacter pasteurianus AP01.

Vinegar is a common food additive produced by acetic acid bacteria (AAB) during fermentation process. Low yield and long incubation time in conventional vinegar fermentation processes has inspired research in developing efficient fermentation techniques by the activation of AAB for acetic acid production. The present study intends to enhance vinegar production using acetic acid bacteria and light emitting diode (LED). A total of eight acetic acid bacteria were isolated from Korean traditional vinegar and assessed for vinegar production. Isolate AP01 exhibited maximum vinegar production and was identified as Acetobacter pasteurianus based on the 16S rRNA sequences. The optimum fermentation conditions for the isolate AP01 was incubation under static condition at 30 °C for 10 days with 6% initial ethanol concentration. Fermentation under red LED light exhibited maximum vinegar production (3.6%) compared to green (3.5%), blue (3.2%), white (2.2%), and non-LED lights (3.0%). Vinegar produced using red LED showed less toxicity to mouse macrophage cell line (RAW 264.7) and high inhibitory effects on nitric oxide and IL-6 production. The results confirmed that red LED light could be used to increase the yield and decrease incubation time in vinegar fermentation process.

Isolation of Lactiplantibacillus sp. from Korean salted and fermented seafoods for effective fermentation of strawberry leaf extract: enhanced anti-inflammatory activity.

Berries are rich in bioactive phytochemicals and phenolic compounds. In the present study, strawberry leaves obtained from Nangsan-myeon, Jeollabuk-do, Korea in 2019 were fermented using Lactiplantibacillus plantarum B1-4 and studied for antioxidant and anti-inflammatory properties. Comparative testing of active ingredients in the raw and fermented extract showed an increase in total polyphenol content and total flavonoid content from 92.0 mg GAE/g and 40.4 mg QE/g, respectively, to 116.1 mg GAE/g and 49.5 mg QE/g, respectively, in fermented extracts. Similarly, catechin content in fermented extract was increased by 26.5% and epicatechin content was decreased by 9.3%. Total and reducing sugar contents in the fermented extract were decreased by 58.4% and 50.4%. DPPH radical scavenging activity of the extracts before and after fermentation increased by about 10.7% from 35.6 to 46.3% at 250 µg/mL and ABTS by about 6.0% from 48.6 to 54.6% at 500 µg/mL. Cytotoxicity assay confirmed that fermented extract caused no harm to chromatid structure of RAW 264.7 cells up to 500 µg/mL concentration. Fermented extracts (400 µg/mL) reduced nitric oxide production (9.7%) and the levels of TNF-α (18.1%) and IL-6 (11.8%), making them ideal for integration into skin care products. The significant functional groups present in raw and fermented extracts were identified using FTIR. Thus, this study adds to the notion of using fermented extracts in functional foods due to their anti-inflammatory properties.

Effects of Crack Tip Constraint on the Fracture Toughness Assessment of 9% Ni Steel for Cryogenic Application in Liquefied Natural Gas Storage Tanks.

Recently, increasing demand for the accurate assessment of the structural integrity and fitness-for-service (FFS) analysis of engineering structures has elevated constraint effects to one of the most important issues in fracture mechanics and structural integrity research. In this paper, the effect of crack tip constraints are investigated on the fracture toughness assessment of 9% Ni steel for application in liquefied natural gas storage tanks. Crack tip opening displacement (CTOD) tests were conducted using both conventional standard three-point bending (3PB) and wide plate (WP) specimens at a cryogenic temperature of -196 °C. The distribution of the stress and strain fields near the crack tip in the 3PB and WP specimens were then obtained by FE (Finite Elements) analysis. Based on both the experimental and numerical results, the parameters of the Weibull distribution were obtained to evaluate the critical Weibull stress at brittle fracture. The equivalent CTOD ratio ß is defined as the ratio of the CTOD of the 3PB specimen to the CTOD of the WP specimen at the same Weibull stress. The application of the proposed CTOD toughness correction method to the WP results was then demonstrated in the context of a failure assessment diagram (FAD). It was determined that the conventional evaluation yields an excessively conservative result for WP specimens, but can be reasonably reduced by applying ß.

Enhanced removal of bisphenol A from contaminated soil by coupling Bacillus subtilis HV-3 with electrochemical system.

Exposure to endocrine disruptors interferes with the synthesis, release, transport and metabolic activities of hormones, thus impairing human health significantly. Bisphenol A (BpA), an endocrine disruptor, commonly released into the environment by industrial activities and needs immediate attention. This study aims at investigating the process and prospects of deploying bio-electrochemical systems (BES) for the removal of BpA from artificially contaminated soil using Bacillus subtilis HV-3. The BES was setup with desired operating conditions: initial concentration of BpA (80-150 mg/L), pH (3-11) and applied potential voltage (0.6-1.4 V). Under optimized conditions (initial BpA concentration, 100 mg/L; pH 7; and applied voltage 1.0 V), close to 98% degradation of BpA was achieved. The intermediates produced during degradation were analysed using High performance liquid chromatography-Mass spectrometry and the possible degradation pathway was elucidated. Phytotoxicity studies in the remediated soil with Phaseolus mungo confirmed the environmental applicability of the BES system.

Agricultural waste materials enhance protease production by Bacillus subtilis B22 in submerged fermentation under blue light-emitting diodes.

Bacillus bacteria have major utility in large-scale production of industrial enzymes, among which proteases have particular importance. B. subtilis B22, an aerobic and chemotrophic strain, was isolated from kimchi and identified by 16S rRNA gene sequencing. Extracellular protease production was determined in basic medium, with 1% (w/v) casein as substrate, by submerged fermentation at 37 °C under blue, green, red and white light-emitting diodes (LEDs), white fluorescent light and darkness. Fermentation under blue LEDs maximized protease production (110.79 ± 1.8 U/mL at 24 h). Various agricultural waste products enhanced production and groundnut oil cake yielded the most protease (334 ± 1.8 U/mL at 72 h). Activity and stability of the purified protease were optimum at pH 7-10 and 20-60 °C. Activity increased in the presence of Ca2+, Mg2+ and Mn2+, while Fe2+, Zn2+, Co2+ and Cu2+ moderated activity, and Ni2+ and Hg2+ inhibited activity. Activity was high (98%) in the presence of ethylenediaminetetraacetic acid (EDTA) but inhibited by phenylmethanesulfonyl fluoride (PMSF). The protease was unaffected by nonionic surfactants, tolerated an anionic surfactant and oxidizing agents, and was compatible with multiple organic solvents. These properties suggest utility of protease produced by B. subtilis B22 under blue LEDs for industrial applications.

Enhanced bioactivity of Zanthoxylum schinifolium fermented extract: Anti-inflammatory, anti-bacterial, and anti-melanogenic activity.

Fermented extracts have evolved to be a potential alternative to synthetic chemicals, owing to their anti-inflammatory and anti-bacterial properties. This study intends to assess the potential of fermented Zanthoxylum schinifolium extract for use in biomedical applications. Probiotic bacteria, Lactobacillus rhamnosus A6-5, were deployed as a seed culture for fermentation. The fermented extract showed greater tyrosinase inhibitory activity and reduced melanin production (58.3%) compared with the raw extract. Cytotoxicity assay inferred that 500 µg/mL is the ideal non-toxic concentration with maximum cell viability. In addition, DAPI staining did not show any damage to the chromatin structure of the cells. The anti-aging property of the fermented extract was confirmed by a decrease in IL-6 content. The fermented extract showed lower MIC (40 mg/mL) and MBC (60 mg/mL), indicating greater anti-bacterial activity than the raw extract. The results confirmed that the fermented Z. schinifolium extract has high biomedical properties compared with the raw extract and can be used as an ideal skin whitening agent.

Effect of panchakavya (organic formulation) on phytoremediation of lead and zinc using Zea mays.

Chelate-assisted phytoextraction is proposed to be an effective approach for the removal of metals from contaminated soil. Organic chelators can improve this biological technique by increasing metal solubility. The aim of this study was to investigate the possibility of improving the phytoextraction of lead (Pb) and zinc (Zn) by the application of panchakavya, a traditional Indian organic formulation. Panchakavya was prepared by fermentation process in open environment using cow dunk, cow ghee, cow urine, cow milk, cow curd, tender coconut water, crude jaggery, and mashed bananas. Soil metal fraction studies indicate that the panchakavya treatment decreased (73%) water-soluble fraction of Pb. Plant growth analysis indicated the application of panchakavya to increase Zea mays fresh root weight, shoot biomass and superoxide dismutase level in Zn contaminated soil. Similarly, a significant increase in the Zn accumulation (12% in shoots and 9% in roots) was observed in panchakavya treated plants. However, when compared to control plants, panchakavya treatment significantly decreased (32% in shoots and 37% in roots) Pb accumulation in Z. mays. Obtained results point out that panchakavya could potentially increase the phytoremediation of Zn in Z. mays.

Remediation of BTEX and Cr(VI) contamination in soil using bioelectrochemical system-an eco-friendly approach.

Soil contamination with benzene, toluene, ethylbenzene and xylene isomers (BTEX) has raised increasing concern because of its high solubility in water and toxicity to biotic communities. This study aims at investigating the process and prospects of deploying bioelectrochemical system (BES) for the removal of BTEX from artificially contaminated soil using Pseudomonas putida YNS1, alongside the reduction of hexavalent chromium (Cr(VI)). The BES was setup with desired operating conditions: initial concentration of BTEX (50-400 mg/L in 100 mL of sterilized water), pH (4-10) and applied potential voltage (0.6-1.2 V) with 10 µL log-phase culture along with the addition of Cr(VI) (10 mg/L). Samples were collected at regular intervals and analysed for BTEX degradation using gas chromatography and Cr(VI) reduction using UV-Vis spectrophotometer. Under optimized conditions (initial BTEX concentration, 200 mg/L; pH 7; and applied voltage 0.8 V with Cr(VI) of 10 mg/L), 92% of BTEX was degraded and 90% Cr(VI) was reduced from the contaminated soil. The intermediates produced during degradation were analysed through gas chromatography-flame ionization detector (GC-FID), and the possible degradation pathway was elucidated. The results indicated that BES could be effective for simultaneous degradation of BTEX along with Cr(VI) reduction.

Role of thermophilic bacteria (Bacillus and Geobacillus) on crude oil degradation and biocorrosion in oil reservoir environment.

Thermophilic bacterial communities generate thick biofilm on carbon steel API 5LX and produce extracellular metabolic products to accelerate the corrosion process in oil reservoirs. In the present study, nine thermophilic biocorrosive bacterial strains belonging to Bacillus and Geobacillus were isolated from the crude oil and produced water sample, and identified using 16S rRNA gene sequencing. The biodegradation efficiency of hydrocarbons was found to be high in the presence of bacterial isolates MN6 (82%), IR4 (94%) and IR2 (87%). During the biodegradation process, induction of the catabolic enzymes such as alkane hydroxylase, alcohol dehydrogenase and lipase were also examined in these isolates. Among them, the highest activity of alkane hydroxylase (130 µmol mg-1 protein) in IR4, alcohol dehydrogenase (70 µmol mg-1 protein) in IR2, and higher lipase activity in IR4 (60 µmol mg-1 protein) was observed. Electrochemical impedance spectroscopy and X-ray diffraction data showed that these isolates oxidize iron into ferrous/ferric oxides as the corrosion products on the carbon steel surface, whilst the crude oil hydrocarbon served as a sole carbon source for bacterial growth and development in such extreme environments.

Enhanced amylase production by a Bacillus subtilis strain under blue light-emitting diodes.

A chemotrophic, aerobic bacterial strain, Bacillus subtilis B2, was used to produce amylase by submerged fermentation under different light sources. SDS-PAGE indicated that the 55 kDa enzyme belonged to the α-amylase group. B2 was incubated in basal media with 1% soluble starch (pH 7.0) under blue, green, red, and white light-emitting diodes (LEDs), and white fluorescent light. Fermentation under blue LEDs maximized amylase production (180.59 ± 1.6 U/mL at 24 h). Production at 48 h increased to 310.56 ± 1.6 U/mL with 5% glucose as a simple carbon source and to 300.51 ± 1.7 U/mL with 5% groundnut oil cake as an agricultural waste substrate. Activity and stability of the amylase were greatest at pH 7.0 and 45-55 °C. Na+, Ca2+, Mg2+, Co2+, Ba2+, and K+ increased activity, while Ni2+, Hg2+, Mn2+, Cu2+, Fe3+, and Zn2+ inhibited activity. EDTA, PMSF and DTNB reduced activity by 50% or more, while tetrafluoroethylene and 1,10-phenanthroline reduced activity by 30%. The amylase was highly tolerant of the surfactants, compatible with organic solvents, oxidizing agents and the reducing agents reduced activity. These properties suggest utility of amylase produced by B. subtilis B2 under blue LED-mediated fermentation for industrial applications.