Relation between voluntary physical activity and oxidant/antioxidant status in rats.

The relationship between voluntary distance running and antioxidant capacity was studied in rats after three weeks voluntary running. Hydroxyl radical level, reduced glutathione level, activities of glutathione reductase and superoxide dismutase were measured in plasma, liver, brain, soleus and gastrocnemius white muscle. Hydroxyl radical level of liver negatively correlated with the running distance (r=-0.616, P<0.001). The reduced glutathione levels of liver and brain increased depending on the running distance and the correlation was confirmed between them in liver (r=0.638, P<0.01) and brain (r=0.766, P<0.001). The hydroxyl radical level in liver positively correlated with the activities of glutathione reductase (r=0.464, P<0.05) and superoxide dismutase (r=0.549, P<0.05). A significant positive correlation was detected between the hydroxyl radical level and superoxide dismutase activity in brain (r=0.488, P<0.05). These results demonstrate that physical activity correlates well with glutathione level and anti-oxidant enzyme activities in liver, suggesting a close relation between physical activity and induction of antioxidant systems.

Effects of pre-exercise listening to slow and fast rhythm music on supramaximal cycle performance and selected metabolic variables.

We examined the effect of listening to two different types of music (with slow and fast rhythm), prior to supramaximal cycle exercise, on performance, heart rate, the concentration of lactate and ammonia in blood, and the concentration of catecholamines in plasma. Six male students participated in this study. After listening to slow rhythm or fast rhythm music for 20 min, the subjects performed supramaximal exercise for 45 s using a cycle ergometer. Listening to slow and fast rhythm music prior to supramaximal exercise did not significantly affect the mean power output. The plasma norepinephrine concentration immediately before the end of listening to slow rhythm music was significantly lower than before listening (p < 0.05). The plasma epinephrine concentration immediately before the end of listening to fast rhythm music was significantly higher than before listening (p < 0.05). The type of music had no effect on blood lactate and ammonia levels or on plasma catecholamine levels following exercise. In conclusion, listening to slow rhythm music decreases the plasma norepinephrine level, and listening to fast rhythm music increases the plasma epinephrine level. The type of music has no impact on power output during exercise.

Effect of gender differences and voluntary exercise on antioxidant capacity in rats.

The effects of gender difference and voluntary exercise on antioxidant capacity in rats were evaluated. The subjects were divided into two groups, physically active and sedentary. In the sedentary group, the level of hydroxyl radical in the liver was higher (P<0.001) in male rats than in female rats, however, in the physically active group, the level in male rats was lower (P<0.05) than in female rats. The levels of reduced glutathione (GSH) in physically active males and females were higher compared to those in the sedentary group. The physically active group also showed an increase in antioxidant enzymes, such as glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase activities. The level of liver GSH was higher in physically active females than in physically active males. For both groups, GPx and GR activities in females were significantly higher than in males. These results indicate that female rats have an intrinsically higher antioxidant capacity, which resulted in increased levels of GSH via the glutathione redox cycle and gamma-glutamyl cycle enzymes. The adaptation to altered antioxidant capacity, induced by physical activity, appeared to be affected by gender differences.

Effect of varying light intensity on maximal power production and selected metabolic variables.

This study was designed to examine the effect of exposure to two levels of light intensity (bright; 5000 lux, dim; 50 lux) prior to supramaximal cycle exercise on performance and metabolic alterations. The exercise was performed after bright and dim light exposure for 90 minutes. Ten male long-distance runners volunteered to take part in the study. They performed 45-sec supramaximal exercise using a cycle ergometer in a 500-lux. Mean power output was measured during the exercise. Lactate and ammonia in the blood and epinephrine and norepinephrine concentrations in plasma were measured at rest immediately after bright and dim light exposures and after the exercise. Bright and dim light exposure prior to exercise did not significantly affect the power output during the exercise. Blood glucose concentration immediately after exercise and plasma epinephrine during the resting period were significantly lower after bright light exposure compared with dim light exposure (p < 0.05). No significant difference was found in blood lactate, ammonia, or plasma norepinephrine levels after exercise following bright and dim light exposures. This study demonstrated that bright light stimulation prior to supramaximal exercise decreases glucose and epinephrine levels, but is not related to physical performance.

Vitamin E supplementation attenuates leakage of enzymes following 6 successive days of running training.

The purpose of this study was to examine whether vitamin E supplementation in humans would attenuate an increase of serum enzymes as an indirect marker of muscle damage following a sudden large increase in the running distance in a 6-day running training or not. A randomized and placebo-controlled study was carried out on fourteen male runners who were supplied vitamin E (alpha-tocopherol 1200 IU x day(-1); E) or placebo (P) 4 weeks prior to (T1) and during 6 successive days of running training (48.3 +/- 5.7 km x day(-1), means +/- SD). Resting venous blood samples were obtained before maximal treadmill running, at T1, the day immediately before (T2), the next day (T3), and three weeks (T4) after the running training. Serum levels of alpha-tocopherol, lipid peroxidation products (thiobarbituric acid; TBA), creatine kinase (CK), lactate dehydrogenase (LDH), and LDH isozyme 1-5 were quantitatively analyzed. No significant difference was found in maximal oxygen uptake (VO2max) and maximal heart rates following the exhaustive exercise between the P and E group during the experiments. Vitamin E supplementation significantly increased serum alpha-tocopherol (p<0.001) and decreased TBA levels (p < 0.001) compared with pre-supplementation levels. Although serum CK and LDH activities increased significantly at T3 in either group, significantly lower CK (p < 0.05) and LDH (p < 0.001) levels were observed in the E group compared with the P group. The ratio of LDH1 to LDH2 (LDH1/LDH2) decreased significantly at T3 in either group compared with the T1 levels, since there was no significant difference in the LDH1/LDH2 between the P and E group throughout the experiments. These results indicate that vitamin E supplementation can reduce the leakage of CK and LDH following 6 successive days of endurance running. The protective effect of vitamin E against free radicals probably inhibits free-radical-induced muscle damage caused by a sudden large increase in the running distance.

Walking at moderate speed with heel-less shoes increases calf blood flow.

This study was designed to examine the physiological and biochemical effects of wearing heel-less shoes over a wide range of walking speeds. Six male students wearing alternately regular shoes and heel-less shoes walked at the constant speeds of 60, 80, 100 and 120 m/min for 10 min on a treadmill at 0% grade. The average heart rate was higher during heel-less shoe trials than when subjects walked in regular shoes at each speed, but differences were not significant. The calf blood flow showed its highest mean value at 80 m/min when subjects walked in heel-less shoes, and at 100 m/min when they walked in regular shoes. However, at walking speeds higher than these, calf blood flow decreased for wearers of both types of shoes. The calf blood flow after 80 m/min was higher when walking in heel-less rather than regular shoes. Blood lactate concentration after walking in heel-less shoes at 120 m/min was significantly higher than basal level, but after walking in regular shoes it was unchanged from the level before walking. Noradrenaline concentration at 120 m/min while walking in heel-less shoes was significantly higher than while walking in regular shoes. In conclusion, walking exercise in heel-less shoes induced an increase of the calf blood flow at a moderate speed, and increased glycogen metabolism and noradrenaline secretion at a faster speed.

Effects of endurance physical training on hydroxyl radical generation in rat tissues.

Physical exercise is known to increase oxygen consumption to compensate for enhanced ATP consumption and thus to induce oxidative stress in tissues. Our previous data indicate that training significantly increased the hydroxyl radical level in rat tissues after physical exercise, but reduced the basal level in aged rats. This result suggests the paradoxical effect of physical training, which may be to increase or scavenge reactive oxygen species (ROS) generated by physical exercise. In this paper, the effects of training on the levels of hydroxyl radicals and the anti-oxidative glutathione system, were examined in rats. After 3 weeks' training, rats were sacrificed at rest or after treadmill running, and the levels of hydroxyl radicals trapped with salicylic acid, reduced (GSH); and oxidized glutathione (GSSG) in tissues were quantified by high-performance liquid chromatography-electrochemical detection (HPLC-ECD). Endurance training reduced the basal level of hydroxyl radicals significantly in plasma and soleus muscle. In liver and brain a similar tendency was observed, but the difference was not statistically significant. In liver the basal level of GSH increased significantly after training. Slower training with longer duration reduced the basal levels of hydroxyl radical in plasma more markedly than more intense training. These results suggest that endurance exercise can increase the anti-oxidative capacity in rats.

Relationship between maximal pulmonary ventilation and arterialized venous blood potassium and dopamine concentrations obtained at exhaustion in man.

This study was performed to test the hypothesis that potassium concentration in arterialized blood may be closely related to maximal pulmonary ventilation (V.Emax) obtained at exhaustion during maximal exercise in man. Eleven healthy men performed bicycle exercise with incremental loading at 60 rpm until exhaustion. Pulmonary ventilation (V.E), oxygen uptake (V.O2), and heart rate (HR) were determined continuously throughout the experiment. Arterialized venous blood samples were collected to measure potassium ([K+]), lactate ([La]), hydrogen ion (pH), catecholamine ([CA]), and dopamine ([DA]) concentrations. A significant correlation (r = 0.98-0.88) between V.E and [K+], [La], and pH during exercise was observed in all subjects. Furthermore, a close relationship was found in this study between dopamine concentration measured at exhaustion ([DA]0) and maximal pulmonary ventilation per kilogram of body weight (V.Emax/W) (r = -0.668, p < 0.05) or maximum oxygen uptake per kilogram of body weight (VO2MAX/W) (r = 0.720, p < 0.05). However, no significant correlation was found between V. Emax/W and [K+]0 (r = 0.202, NS), [La]0 (r = -0.096, NS), and pH0 (r = 0.344, NS). These results suggest that dopamine may play a more important role in the determination of maximal pulmonary ventilation during exercise in man than K+ or pH.

Novel assay for glutathione reductase activity by high-performance liquid chromatography with electrochemical detection.

A new method was devised for the direct measurement of glutathione reductase activity in crude tissue samples using high-performance liquid chromatography and electrochemical detection with a mercury/gold electrode. This assay depends on the high sensitivity of the electrode to detect SH groups produced from a substrate, oxidized glutathione, the interference of which can be excluded by setting the oxidation voltage as low as 100 mV. The sensitivity was high enough to detect the activity in crude samples containing less than 1 microg of protein. This method proved to be sensitive and simple for the measurement of this biologically important reductant activity without the need for a further purification procedure.

Unilateral linear lichen sclerosus et atrophicus.

We report on a 23-year-old woman with unilateral lichen sclerosus et atrophicus (LSA) who exhibited extensive eruptions on the right extremities and right aspect of the abdomen. The lesions on the right extremities appeared to exhibit a linear pattern along Blaschko's lines, while the right abdominal skin lesion was a whitish, parchment-like plaque. Histological findings for both lesions showed characteristic features of LSA. LSA should be differentiated from the linear type and the macular type of localized scleroderma.

Glutathione status and reactive oxygen generation in tissues of young and old exercised rats.

The effects of exercise on the generation of active oxygen species and radical-scavenging capacity were studied in physically active and sedentary young and old rats. Exercise increased the hydroxyl radical content in all tissues of physically active young rats, except in the plasma. In old rats, the basal level of the radical increased significantly in plasma, heart, and skeletal muscles, but decreased in liver; and physical activity decreased it to that of young rats in most cases. With exercise, the content of reduced glutathione increased in plasma, heart, and skeletal muscles of young rats, whereas that of oxidized glutathione markedly decreased in liver and increased in brain and white gastrocnemius muscle. The total glutathione levels in these tissues changed in a similar way, indicating that glutathione was released from the pool in the liver. In rats allowed to run voluntarily for 5 weeks, the effects were more pronounced than in the sedentary rats. The ratio of reduced to total glutathione, which indicates the capacity to reduce glutathione, increased in plasma, heart, and soleus muscle of sedentary young rats after exercise, and increased further in those undergoing physical activity. In old rats, the reduced glutathione level increased in plasma, heart, liver, and brain, even though the total decreased. These results suggest that physical activity enhances the endogenous ability to defend against oxidative stress. In old rats, even though glutathione synthesis is decreased, the regenerating capacity seems to be increased in order to compensate for the increased oxidative stress.

A simulation study of the electromyographic volley at initiation of rapid isometric contractions in the first dorsal interosseous muscle.

Monopolar surface electromyograms (EMGs) of rapid isometric abduction of the first dorsal interosseous muscle (FDI) were initiated from an EMG volley that was characterized by a negative potential lasting over several tens of milliseconds. An EMG model was developed to study how the EMG volley was generated. EMGs were defined as the linear summations of surface-recorded action potential trains originating from single motor units (MUs). All action potential trains had the same discharge pattern but different recruitment thresholds, depending on the potential amplitude. Real action potentials in single MUs in FDI were recorded with a monopolar surface electrode, one of which was used as a prototype wave in simulation. The model predicted an initial negative potential comparable to that of the EMG volley observed in rapid contractions of FDI. Results from our simulation studies suggest that the EMG volley is caused by at least two independent factors: (1) the negative phase of the action potential is greater in area than the positive one, in which the effect is enhanced by the high discharge rate of many MUs; (2) many MUs are recruited within a short time in an orderly fashion starting from those with small action potentials to those with large ones.

Human blood lactate and ammonia levels after supramaximal uphill and downhill running.

The purposes of this study were 1) to confirm whether there is a difference in the levels of blood lactate and ammonia after supramaximal uphill and downhill running for the same short duration and 2) to examine the relationship between peak blood lactate levels and work/lean body mass (LBM), as well as the relationship between peak blood ammonia levels and work/LBM following supramaximal uphill and downhill running. Eight healthy, untrained male subjects performed supramaximal uphill and downhill running on a motor-driven treadmill for about 70 sec. Though there was a significant difference (p < 0.05) in running speed and work/LBM between supramaximal uphill and downhill running, no significant difference was found in exhaustion time or heart rate. Both the peak blood lactate and ammonia concentrations were significantly lower after downhill running than after uphill running (p < 0.05). Although there was no significant relationship between peak blood ammonia levels and work/LBM following either uphill or downhill running, significant linear relationships between the peak blood lactate levels and work/LBM were observed following uphill running (r = 0.74, p < 0.05) and downhill running (r = 0.72, p < 0.05). These results suggest that the differences in the blood lactate and ammonia concentration between supramaximal downhill and uphill running of the same duration may be due to the total recruitable muscle mass during exercise, and that peak blood lactate can be used as an index of anaerobic work capacity for untrained subjects under these running conditions.

Salivary and blood lactate after supramaximal exercise in sprinters and long-distance runners.

This investigation examined the reproducibility of salivary lactate measurement in long-distance runners following a 3000-m run and compared the salivary and blood lactate concentrations in sprinters and long-distance runners following a 400-m run. There was no significant difference in salivary and blood lactate between the first and second 3000-m run test. The peak salivary and blood lactate following a 400-m run was higher in sprinters than in long-distance runners. There was a significant relationship between the peak lactate of saliva and that of blood following a 400-m run. The results showed that the salivary lactate following exercise was reproducible and the salivary lactate concentration may serve as a relevant indicator in determining which have a higher lactate production.

The influence of arm movements on a vertical jump.

The influence of arm lowering movements (ALM) on a vertical jump with all but the ankle joints immobilized was studied in five healthy volunteers. Subjects were instructed to rapidly lower both arms after takeoff when jumping from an initial position with the elbows flexed. They performed the jumps either with their hands empty or holding 4 kg dumbbells. ALM prolonged the time subjects were airborne, especially when holding the dumbbells. Protocol for the experiment required that subjects maintain maximum angular velocity of the ankle joint, vertical platform force, and electromyographic monitoring from the triceps surae muscle. Positive acceleration of the head increased in the ALM jumps, and became even more pronounced when the jumps were performed with dumbbells. This increase in head acceleration was inversely proportional to the downward acceleration of the hands. These results indicate that prolonging a vertical jump depends on ALM in the air.

Effect of immobilization on glucose transport and glucose transporter expression in rat skeletal muscle.

The effect of 42-48 h of immobilization by casting on maximal velocity of 3-O-methylglucose (3-MG) transport in skeletal muscle was studied in the perfused rat hindquarter. Immobilization resulted in a decrease of approximately 42% for maximum insulin-stimulated 3-MG transport in fast-twitch red fibers and a decrease of approximately 42% for contraction-stimulated transport in slow-twitch red fibers compared with nonimmobilized control muscle. No effect of immobilization on 3-MG transport was found in fast-twitch white muscle. Combination of insulin and muscle contractions always resulted in glucose transport that was identical in immobilized and control muscle. Western blot did not detect a decrease in GLUT-1 or GLUT-4 protein with immobilization. Furthermore, in fast-twitch red fibers, insulin receptor number and receptor kinase activity did not differ between immobilized and control muscle. It is concluded that during short-term immobilization a resistance of muscle glucose transport to stimulation develops that is fiber type specific and selective for insulin or contractions. The resistance can be overcome by the combined action of insulin and contractions and reflect factors other than glucose transporter number and insulin receptor binding and receptor kinase activity.

Muscle activation in the elbow-forearm complex during rapid elbow extension.

Normal human subjects performed extensions of the elbow from an initial position to a visually defined target as rapidly and accurately as possible at different angular amplitudes (9 degrees-54 degrees) and at a constant amplitude (36 degrees) under various additional instructions. Joint angle, angular acceleration, and electromyograms (EMGs) from agonist (triceps lateralis), antagonist (biceps), and antebrachial muscles were recorded. EMGs of the antagonistic muscles showed reciprocal activation of the first triceps, and then the biceps, which was followed by the second triceps activation and coactivation of both muscles. Movement amplitude changed the reciprocal activation, whereas the coactivation was unaltered. Additional instructions changed the coactivation in amplitude, initiation, and termination, whereas the reciprocal activation was relatively preserved. Under these conditions, EMGs of antebrachial muscles were always linked with the coactivation in amplitude and timing but not with the reciprocal activation. The linkage suggests that antebrachial muscle activation serves as an indicator of the coactivation. From the indicator, we infer that the coactivation initiates from the beginning of the rapid movements and changes during the dynamic movement phase.

Hydroxyl radical formation in diabetic rats induced by streptozotocin.

Production of hydroxyl radicals was examined in the diabetic rats induced by streptozotocin to prove its involvement to the pathogenesis of diabetes. Hydroxyl radicals generated in plasma, heart muscle, liver and brain of the hyperglycemic rats were quantitatively assayed by trapping hydroxyl radicals with salicylic acid as 2,3- and 2,5-dihydroxybenzoic acid. The concentrations of 2,3- and 2,5-dihydroxybenzoic acid were significantly increased in all the tissues of the diabetic rats. In the brain and heart muscle of the diabetic rats, the increase of 2,3-dihydroxybenzoic acid was more manifest than that of 2,5-dihydroxybenzoic acid, while in liver 2,5-dihydroxybenzoic acid increased markedly. All the values of 2,3-dihydroxybenzoic acid detected in the tissues of the diabetic rats were quite higher than those in control. Hydroxyl radical production and blood glucose concentration were depended almost linearly on the amount of streptozotocin injected to rats up to 60 mg/kg body weight. It was suggested that 2,3-dihydroxybenzoic acid was produced from hydroxyl radicals themselves, while 2,5-dihydroxybenzoic acid was produced by hydroxylation of salicylic acid not only with hydroxyl radicals, but also by enzymatic reaction of microsomal cytochrome-P450. Hydroxyl radical formation may account for some pathological process especially in the heart muscle and brain.

Relationship between maximal pulmonary ventilation during exhaustive exercise and postexercise plasma potassium concentration in man.

The present study was undertaken to examine the relationship between maximal pulmonary ventilation (VEmax) and potassium concentration ([K+]) or lactate concentration ([La]) of venous blood in man. Nine healthy men performed exercise on a bicycle ergometer at a constant rate of 60 rpm until volitional fatigue. VEmax ranged from 100.4 to 153.2 l/min. In all subjects, potassium concentration measured at 1 min ([K+]1.0) after maximal exhaustive exercise was the highest, and it returned quickly to the resting level within a few minutes during recovery. VEmax was significantly correlated (r = 0.750, p < 0.05) to [K+]1.0, but not to lactate concentration determined at 1 min ([La]1.0) during recovery. These results suggest that a higher increase in blood [K+] may, at least partly, contribute to a greater augmentation of VE during exhaustive exercise.

Reciprocal activation and coactivation in antagonistic muscles during rapid goal-directed movements.

Seven normal subjects performed elbow extensions as rapidly as possible from an initial position to a visually defined target at 36 degrees in amplitude. In electromyograms, the reciprocal activation of the agonist and then antagonist bursts was always followed by simultaneous activation of the antagonistic muscles, i.e., coactivation. Instructions added to perform extensions "as rapidly as possible" changed coactivation; the command to "strongly fix the upper arm at the target" increased coactivation, whereas "relax immediately after the start of movement" made coactivation almost disappear. However, basic features of reciprocal activation remained the same. Other instructions given also changed coactivation on initiation and termination, while reciprocal activation was relatively unaltered. When subjects were encouraged to "relax immediately after the start of movement, but fix the upper arm quickly after attaining the target," coactivation initiated shortly after reaching the target (< 200 ms). Following the instruction to "relax the upper arm quickly after attaining the target," coactivation terminated rapidly after reaching the target (< 280 ms). The results show that instructions serve to change amplitude and timing of coactivation while keeping reciprocal activation relatively unaltered, suggesting that coactivation is controlled independently of reciprocal activation during rapid goal-directed movements.