The impact of negative factors of professional activities on the health of law enforcement officers.

OBJECTIVE: Aim: To study the study the impact of negative factors of professional activities on the health of law enforcement officers. PATIENTS AND METHODS: Materials and Methods: The research involved 89 law enforcement officers (58 men, and 31 women) who served for a year in practical police units after graduating from a higher educational institution. Methods: analysis and generalization of scientific and methodological literature, anthropometry, physiometry, testing, and statistical methods. The health status was assessed by body mass index, Robinson index, and vital index. RESULTS: Results: It was found that the body mass index of law enforcement officers for one year of service in practical units deteriorated in both men and women by 1.3 kg/m2 and 0.9 kg/m2, respectively. More negative changes were observed in men. At the same time, after one year of service, the number of men with overweight (27.5 %) and even obesity of the first (13.9 %) and second (5.2 %) degrees increased significantly. In women, the changes were less pronounced. The dynamics of Robinson and vital indices, as well as the level of physical fitness, were also negative. CONCLUSION: Conclusions: The results of the research indicate a negative impact of professional factors in terms of professional activities on the health of law enforcement officers. It has been found that adherence to the principles of a healthy lifestyle, in particular, a regimen of rationally organized motor activity, is an important area in combating the impact of negative factors of professional activities on the health of law enforcement officers.

Motivational and value-based attitude of law enforcement officers to motor activity as a factor of a healthy lifestyle.

OBJECTIVE: Aim: To study the attitude of law enforcement officers to motor activity and investigate its impact on their health. PATIENTS AND METHODS: Materials and Methods: The research, which was conducted in 2022-2024, involved 163 law enforcement officers under the age of 30. Research methods: bibliosemantic, questionnaire, testing, medical and biological methods, methods of mathematical statistics. RESULTS: Results: It has been found that the vast majority of law enforcement officers (85.3 %) understand the importance of motor activity as a factor of a healthy lifestyle, its impact on physical and mental health, and quality of their professional activities. At the same time, only 27.6 % of law enforcement officers systematically engage in motor activity, 52.8 % do it sporadically, and 25.8 % do not engage in it at all. Among the reasons that prevent them from exercising are lack of time (65.6 %), lack of desire (31.3 %), and fatigue after a service day (28.1 %). It has been found that law enforcement officers who systematically engage in motor activity have a significantly better level of health (7.31 points) compared to those who engage in occasional motor activity (5.07 points) and do not engage at all (2.19 points). CONCLUSION: Conclusions: The positive impact of motor activity on the level of health of law enforcement officers has been proved. Good health is a guarantee of high resistance of law enforcement officers to negative factors of professional activities, professional longevity, and quality of their professional tasks.

Professional health of instructor-officers with different service experience.

OBJECTIVE: Aim: To investigate the levels and indicators of professional deformation in instructor-officers with different service experience as a negative factor in their professional health. PATIENTS AND METHODS: Materials and Methods: The research, which was conducted in 2022-2023, involved 136 instructor-officers of the National Academy of Internal Affairs aged 25-55 years with different service experience (up to 5 years - 21 people, 5-10 years - 45 people, 10-15 years - 42 people, over 15 years - 28 people). RESULTS: Results: The dynamics of the levels of professional deformation in instructor-officers of higher educational institutions depending on the experience of their service activities have been determined, and the peculiarities of the manifestation of key professional and psychological indicators that affect their professional health in the course of their service activities have been revealed. CONCLUSION: Conclusions: Professional deformation can be manifested at the initial, middle, and deep levels. The experience of service activities makes adjustments to the formation of professional deformation in instructor-officers because deformational changes in personality occur under the constant influence of the conditions of the profession. With the increase in experience, they become more adapted to stressful phenomena and tense situations of service activities, but the degree of desire to change the type of activity increases. The decrease in proactive attitude and selectivity of contacts during the performance of professional tasks is often accompanied by exhaustion, which indicates a progressive tendency to the development of deformational changes in instructor-officers.

Substantiation of the methodology for assessing the biological age of adolescents.

OBJECTIVE: Aim: To substantiate the methodology for assessing the biological age of adolescent high schoolers (boys and girls). PATIENTS AND METHODS: Materials and Methods: Boys aged 14-15 (n = 102) and girls aged 12-13 (n = 101) were studied. The choice of these age groups is explained by the presence of the most significant individual differences in morphological and functional characteristics in these adolescents. Methods: anthropometry, somatometry, somatoscopy, tonometry, spirometry, dynamometry, Stange's test, Rufier and Robinson indices. The interaction of the results of different methods of assessing biological age was conducted on one sex-age sample using factor analysis (principal components method). RESULTS: Results: It has been established that the biological age of adolescents can be reliably determined using a set of three components: an assessment of the level of physical development, the index of heterochronicity, and the degree of manifestation of secondary sexual characteristics (for boys with a probability of 90.2 %, for girls - 92.1 %). The percentage ratio of these components has sexual peculiarities. Methodologies for assessing biological age for scientific purposes and rapid assessment for use in the practice of physical education and sports have been proposed. The indicators of rapid assessment are body length and the degree of hair development in the axilla. The reliability of the rapid assessment is 81.4 % for boys and 83.2 % for girls. CONCLUSION: Conclusions: Developing adequate physical activities for adolescents of different biological ages will help improve the physical health of the younger generation.

Motivational orientations of students towards internet dependent behavior and measures for its prevention.

OBJECTIVE: Aim: The aim is to study the motivation of students towards Internet dependent behavior and develop practical recommendations for improving a set of measures for its prevention. PATIENTS AND METHODS: Materials and Methods: The research involved 154 students of the National Academy of Internal Affairs. Research methods: analysis and generalization of literature sources, questionnaire, statistical methods. RESULTS: Results: The priority motives of students who manifest Internet dependence behavior were identif i ed. The motivational orientations of students determine their systematic stay in the virtual environment and include, first of all, compliance with modern world trends; accessibility of content; the need for recognition of personal results by other users; satisfaction with virtual communication with the social environment. CONCLUSION: Conclusions: The practical recommendations for improving a set of measures to counteract the spread of Internet dependence among students were developed. Overcoming Internet dependence involves influencing a person to change his or her motivational and value as well as communication spheres. Prevention of Internet dependence involves public health professionals conducting awareness-raising as well as psychological and correctional work with the most vulnerable categories of people.

Self-assessment of somatic and mental health by instructor-officers under the influence of negative factors of professional activities.

OBJECTIVE: Aim: To investigate the impact of negative factors of professional activities in the context of the COVID-19 pandemic and martial law in Ukraine on the somatic and mental health of instructor-officers with different teaching experiences. PATIENTS AND METHODS: Materials and Methods: The research involved 131 instructor-officers with different teaching experiences. Three groups of instructor-officers were formed: Group 1 - instructors with up to 10 years of teaching experience (n = 30), Group 2 - instructors with 10 to 20 years of experience (n = 47), Group 3 - instructors with more than 20 years of experience (n = 54). The self-assessment of somatic and mental health was carried out using a questionnaire developed by the authors. RESULTS: Results: The negative impact of the factors of professional activities in the context of the COVID-19 pandemic and martial law in Ukraine on the somatic and mental health of instructor-officers was established. More than 50 % of the instructors rated their health as satisfactory, and more than 20 % as poor. The overwhelming majority of instructors reported a slight (over 40 %) and significant (over 30 %) deterioration in both somatic and mental health. Health problems over the past year have interfered with the performance of professional duties for more than 80 % of instructor-officers. CONCLUSION: Conclusions: The systematic impact of negative factors can lead to some psychosomatic disorders and diseases in instructor-officers and a decrease in the quality of their teaching activities. This necessitates the search for ways to restore the somatic and mental health of instructor-officers.

Changes in somatic and mental health indicators of instructor-officers under stress.

OBJECTIVE: Aim: To investigate changes in the indicators of somatic and mental health of instructor-officers in higher educational institutions with specific learning environment under stress. PATIENTS AND METHODS: Materials and Methods: The research involved instructor-officers of the National Academy of Internal Affairs (Ukraine, n = 86) with different teaching experiences: up to 10 years (group 1, n = 22), from 10 to 20 years (group 2, n = 31), over 20 years (group 3, n = 33). The study of somatic and mental health was carried out in September-October 2021 (Stage 1) and in September-October 2023 (Stage 2). RESULTS: Results: Significantly negative changes in the indicators of somatic and mental health of instructor-officers with different experiences of teaching under stress were found. The most pronounced negative changes were found in the body weight index, vital index, strength index, Martin-Kushelevsky index, and emotional exhaustion of instructors. Among the studied groups of instructor-officers, the greatest changes occurred in instructors with 10 to 20 years of experience and more than 20 years, and the least - in instructors with up to 10 years of experience. CONCLUSION: Conclusions: It was found that the professional activities of instructor-officers, which take place under stress, negatively affect both the somatic and mental health of instructors, regardless of their teaching experience. Such activities lead to an increase in body weight, deterioration of the functioning of the main body systems, and increased professional burnout of instructors. This can lead to several psychosomatic disorders and diseases in instructor-officers and a decrease in the quality of their teaching activities.

Small-angle X-ray scattering structural insights into alternative pathway of actin oligomerization associated with inactivated state.

In addition to the well-known monomeric globular (G-actin) and polymeric fibrillar (F-actin) forms, actin can exist in the so-called inactivated form (I-actin). Hsp70 chaperon, prefoldin, and CCT chaperonin are required to obtain native globular state. In contrast, I-actin is spontaneously formed in the absence of intracellular folding machinery. I-actin can be obtained from G-actin by elimination of divalent ion, incubation in presence of small concentrations of denaturants, and by heat exposure. Since G-actin is a quasi-stationary, thermodynamically unstable form, it can gradually transform into inactivated state in the absence of chelating/denaturating agents or heat exposure, but the transition is much slower. I-actin was shown to associate into oligomers up to the molecular weight of 14-16 G-actin monomers, though the structure of these oligomers remains uncharacterized. This study employs small-angle X-ray scattering to reveal novel insights into the oligomerization process of such spontaneously formed inactivated actin. These oligomers are differentiated from F-actin through comparative analysis, highlighting a unique oligomerization pathway.

CADETS' MOTIVATION FOR MOTOR ACTIVITY AS AN IMPORTANT FACTOR IN IMPROVING THEIR HEALTH.

OBJECTIVE: Aim: To investigate cadets' motivational priorities for physical exercises and sports in the process of their training and the dynamics of their health indicators. PATIENTS AND METHODS: Materials and methods: The research was conducted at the National Academy of Internal Affairs in 2018-2022. The research of motivational priorities involved 372 male cadets from the 1st to the 4th instructional years aged 17 to 22. Two groups were formed to investigate the state of cadets' health: group A (n = 39) included motivated cadets who were additionally engaged in sports, group B (n = 66) was represented by insufficiently motivated cadets who undertook training according to the traditional physical education program and did not additionally engage in physical exercises and sports. RESULTS: Results: It was found that the motivational priorities of cadets for physical exercises and sports vary depending on the instructional year. Group A cadets showed a significantly better level of health than those from group B, by 2.98 points (p ≤ 0.001) during the 4th instructional year. CONCLUSION: Conclusions: A high level of cadets' health formed in the process of physical exercises and sports during training will contribute to the mastering of the educational program and, in the future, will ensure the effectiveness of their service activities. Therefore, the formation of cadets' conscious motivation for motor activities during training in order to improve their health is an important task of higher educational institutions.

THE IMPACT OF PROFESSIONAL STRESS ON THE MENTAL HEALTH OF LAW ENFORCEMENT OFFICERS.

OBJECTIVE: The aim: To investigate the impact of professional stress on the mental health of law enforcement officers. PATIENTS AND METHODS: Materials and methods: The research involved two groups of law enforcement officers (aged 25-40): the first group with up to 7 years of service activities (n = 52), the second group - 8-15 years (n = 48). Research methods: bibliosemantic, questionnaire, testing, method of system analysis and generalization, mathematical and statistical. RESULTS: Results: It was found that law enforcement officers of both groups identified indicators of a significant professional workload, lack of adequate rest, and the nature of communication with participants in professional relationships among the factors of professional stress. It is proved that the mental health of law enforcement officers deteriorates under the influence of professional stress with the experience of their professional activities. Law enforcement officers with 8-15 years of service experience have significantly (p < 0.05-0.001) worse indicators of anxiety, aggressiveness, motivation, personal detachment, impulsive¬ness, vigor, and work efficiency. CONCLUSION: Conclusions: It was established that the professional stress of law enforcement officers is the result of an imbalance between the internal resources of the individual and the requirements of the external environment, embodied in the peculiarities of law enforcement activities. Law enforcement officers are char¬acterized by the presence of professional stress of average and high severity, which increases with the experience of their service activities. A set of measures was proposed to provide psychological assistance to law enforcement officers to effectively counteract professional stress and improve their mental health.

PECULIARITIES OF FORMATION OF CADETS' PSYCHOLOGICAL RESILIENCE AND PHYSICAL READINESS FOR COMBAT STRESS.

OBJECTIVE: The aim: To investigate the impact of special physical training sessions on the formation of cadets' psychological resilience and physical readiness for the stress factors of future professional and combat activities. PATIENTS AND METHODS: Materials and methods: The research involved 96 cadets (men) in the 2nd training year of S. P. Koroliov Zhytomyr Military Institute (Ukraine) aged 18-20 years, who were divided into two groups: the experimental (E, n = 47) and the control (C, n = 49). The cadets of the E group studied according to the authors' program, and the C group cadets - according to the existing program. Cadets' psychological resilience was studied using psycho-diagnostic methods aimed at assessing their volitional qualities. Cadets' physical fitness was assessed by the tests of general and special physical training. RESULTS: Results: It was found that the level of general physical training of the E and the C group cadets was significantly the same (p > 0.05) at the end of the research but in terms of special physical training and psychological readiness, the E group cadets had all indicators significantly (p < 0.05-0.001) better than those of the C group. CONCLUSION: Conclusions: It has been proved that the special physical training sessions conducted according to the authors' program were more effective than the existing program in forming cadets' physical readiness and psychological resilience for stress factors of future professional and combat activities.

I-Shaped Dimers of a Plant Chloroplast FOF1-ATP Synthase in Response to Changes in Ionic Strength.

F-type ATP synthases play a key role in oxidative and photophosphorylation processes generating adenosine triphosphate (ATP) for most biochemical reactions in living organisms. In contrast to the mitochondrial FOF1-ATP synthases, those of chloroplasts are known to be mostly monomers with approx. 15% fraction of oligomers interacting presumably non-specifically in a thylakoid membrane. To shed light on the nature of this difference we studied interactions of the chloroplast ATP synthases using small-angle X-ray scattering (SAXS) method. Here, we report evidence of I-shaped dimerization of solubilized FOF1-ATP synthases from spinach chloroplasts at different ionic strengths. The structural data were obtained by SAXS and demonstrated dimerization in response to ionic strength. The best model describing SAXS data was two ATP-synthases connected through F1/F1' parts, presumably via their δ-subunits, forming "I" shape dimers. Such I-shaped dimers might possibly connect the neighboring lamellae in thylakoid stacks assuming that the FOF1 monomers comprising such dimers are embedded in parallel opposing stacked thylakoid membrane areas. If this type of dimerization exists in nature, it might be one of the pathways of inhibition of chloroplast FOF1-ATP synthase for preventing ATP hydrolysis in the dark, when ionic strength in plant chloroplasts is rising. Together with a redox switch inserted into a γ-subunit of chloroplast FOF1 and lateral oligomerization, an I-shaped dimerization might comprise a subtle regulatory process of ATP synthesis and stabilize the structure of thylakoid stacks in chloroplasts.

Mirror proteorhodopsins.

Proteorhodopsins (PRs), bacterial light-driven outward proton pumps comprise the first discovered and largest family of rhodopsins, they play a significant role in life on the Earth. A big remaining mystery was that up-to-date there was no described bacterial rhodopsins pumping protons at acidic pH despite the fact that bacteria live in different pH environment. Here we describe conceptually new bacterial rhodopsins which are operating as outward proton pumps at acidic pH. A comprehensive function-structure study of a representative of a new clade of proton pumping rhodopsins which we name "mirror proteorhodopsins", from Sphingomonas paucimobilis (SpaR) shows cavity/gate architecture of the proton translocation pathway rather resembling channelrhodopsins than the known rhodopsin proton pumps. Another unique property of mirror proteorhodopsins is that proton pumping is inhibited by a millimolar concentration of zinc. We also show that mirror proteorhodopsins are extensively represented in opportunistic multidrug resistant human pathogens, plant growth-promoting and zinc solubilizing bacteria. They may be of optogenetic interest.

THE EFFECT OF PHYSICAL TRAINING ON HEALTH AND PSYCHO!EMOTIONAL STATE OF MANAGERS OF LAW ENFORCEMENT AGENCIES.

OBJECTIVE: The aim: To investigate the e!ect of physical training sessions on health and psycho-emotional state of managers of law enforcement agencies. PATIENTS AND METHODS: Materials and methods: The research was conducted in 2019-2021. The research involved 155 managers of law enforcement agencies (men) of different age groups. Research methods: analysis and synthesis of literature, pedagogical observation, testing, methods of mathematical statistics, including correlation analysis (Pearson's correlation coefficient was used). RESULTS: Results: An insufficient level of general physical #tness of managers of law enforcement agencies of all age groups was revealed. The worst level was found among managers of older age groups. Among physical qualities, the worst level was found in the development of endurance. A reliable relationship between the indicators of health and psycho-emotional state of managers of law enforcement agencies and the level of their general physical #tness was revealed. The highest correlation coefficients of these. CONCLUSION: Conclusions: It was established that the use of general physical training with the predominant use of endurance and strength exercises, taking into account the age of managers of law enforcement agencies is the direction of solving the problem of promoting health, improving indicators of psycho-emotional state and professional activities of managers of law enforcement agencies.

MORPHOFUNCTIONAL STATUS OF INSTRUCTORS OF HIGHER EDUCATIONAL INSTITUTIONS DURING THEIR PEDAGOGICAL ACTIVITIES.

OBJECTIVE: The aim: To research the morphofunctional status of instructors of higher educational institutions of di"erent age groups in the process of their pedagogical activities. PATIENTS AND METHODS: Materials and methods: The research was conducted in the period from 2019 to 2021. The research involved 126 instructor offcers (men) of different age groups: under 30 years of age - 21 people, 31-35 years of age - 27 people, 36-40 years of age - 32 people, 41-45 years of age - 27 people, over 45 years of age - 19 people. Morphofunctional status of the instructor offcers was assessed by the indicators of their height, body weight, lung vital capacity, wrist dynamometry, heart rate, blood pressure and relevant indices. RESULTS: Results: It was found that during the study (2019-2020) the Kettle index, vital index, strength index, Robinson index and duration of recovery processes deteriorated among instructor offcers of all age groups. However, in instructor offcers of 36-40, 41-45 and over 45 years of age most of indices deteriorated reliably (P < 0.05). The values of the studied indices are below average or low in most instructors of all age groups, most instructors are overweight. CONCLUSION: Conclusions: It was found that the level of morphofunctional status of the instructional sta" is insuffcient to perform the tasks of their pedagogical activities. Rationally organized health-improving physical training sessions, taking into account the age group, morphofunctional status of instructors and the time of training sessions during the working day can be an effective way to solve this problem.

Improvement of Health and Morphofunctional Status of Law Enforcement Officers of Older Age Groups during the Organized Motor Activities.

BACKGROUND: With age, there is a tendency for the health of law enforcement officers to deteriorate. This especially applies to instructor officers, whose professional activity is characterized by a significant decrease in the volume of motor activity, overloading of the intellectual sphere, and high neuro-emotional stress. OBJECTIVE: The aim of the research is to investigate the influence of recreational and healthpromoting training sessions with physical exercise on the health, physical development, and functional status of instructor officers of older age groups. METHODS: 62 instructor officers (aged 40-45) of various academic disciplines took part in the research. The instructors of the experimental group (EG, n = 30) were engaged in physical exercises in accordance with the authors' program of recreational and health-promoting training sessions; the instructors of the control group (CG, n = 32) attended the training sessions according to the existing physical training program. RESULTS: It was found that at the end of the experiment, the studied indicators of the EG instructor officers were significantly better than those of the CG ones, according to the indicators of body weight, Quetelet index, the vital capacity of lungs, vital index, strength index, heart rate, arterial blood pressure, Robinson index, and level of physical health. CONCLUSION: It was established that systematic recreational and health-promoting training sessions have a positive effect on the health level of the instructor officers of older age groups. This will allow them to carry out instructional activities at a high professional level and ensure resistance to adverse factors of professional activities.

MOTIVATION DEVELOPMENT OF MENTAL HEALTH PRESERVATION OF SPECIALISTS IN THE FIELD OF SPECIAL AND INCLUSIVE EDUCATION: EUROPEAN PRACTICES.

OBJECTIVE: Aim: The aim is to study and to improve the motivation of mental health preservation of specialists in the field of special and inclusive education according to European experiences. PATIENTS AND METHODS: Materials and methods: The experimental part of the research involved the use of the valid psychodiagnostic methods and tech¬niques: direct and indirect observation, standardized questionnaire survey, semi-standardized individual interviews, psychodiag¬nostic methods. The research was attended by 131 Master's degree students (aged 25-27), specialty 053 Psychology, Educational program - special, clinical psychology. RESULTS: Results: The program "European practices of motivation development of mental health preservation" expands perception of ca¬pabilities, which encourages the disclosure of the creative potential of the individual; anxiety symptoms disappear (or decrease); a system of value orientations is formed; the desire to strengthen spiritual and physical strength. CONCLUSION: Conclusions: Program contributes to the formation of an image of mentally healthy person, stimulation to adhere to a mentally healthy lifestyle and the motivation development of mental health preservation.

FORMATION OF PSYCHOPHYSICAL READINESS OF CADETS DURING APPLIED EXERCISES.

OBJECTIVE: Aim: is to study the level and dynamics of the development of physical and psychological qualities of the cadets in the process of engagement in pentathlon classes. PATIENTS AND METHODS: Materials and methods: The research involved 18-23 years old cadets (men) of the first-fourth years of training. The level of the cadets' physical qualities was assessed by their results in the 100 m run, pull-ups, 3 km run, overcoming of the obstacle course. The level of the cadets' psychological qualities was studied using the following methods: well-being-activity-mood methodol¬ogy; methods of self-assessment of psycho-emotional state; methods of determining the level of the development of volitional self-regulation of the individual. RESULTS: Results: The positive influence of pentathlon classes on the development of physical and psychological qualities in the cadets and the improvement of their psycho-emotional state was established. The greatest effect was found in exercises aimed at de-veloping endurance and agility as well as coordination skills. CONCLUSION: Conclusions: It was found that pentathlon classes are more effective than the traditional physical training classes; they affect the level of the development of all cadets' physical qualities. The high level of physical and psychological qualities of the cadets will help to improve their future professional activities.

IMPACT OF MOTOR ACTIVITY ON PROMOTION OF HEALTH AND RESTORATION OF MENTAL CAPACITY OF TEACHING STAFF.

OBJECTIVE: The aim: To find out the impact of exercise on promotion of health and restoration of mental capacity of instructors of higher educational institutions with specific learning environment. PATIENTS AND METHODS: Materials and methods: The research was conducted in 2018-2021. Research methods: theoretical analysis of literature sources, documentary method, pedagogical obser¬vation. The theoretical analysis of literature sources and documentary method allowed to clarify the role, place and objectives of physical training in the professional activities of the instructors; the pedagogical observation was used in the analysis of the instructors' professional activities. CONCLUSION: Conclusions: It was found that the instructors' professional activities are influenced by many negative factors: low motor activity, length of working day and week, forced working position, stress, mental overload, stress, lack of time for physical training. These factors result in the appearance of excess weight, dysfunction of major body systems, reduced mental capacity, the emergence of various diseases and labour costs. All this has a negative impact on the learning process of cadets. The direction for improving the physical training of instructors is to increase the amount of general physical training, conducting training sessions with different orientations taking into account age, physical condition, teaching load of instructors, as well as class time. A high level of instructors' general physical fitness will allow to effectively perform training tasks; strengthen their health; maintain a high level of mental capacity for a long time; extend professional longevity.

Structural basis for receptor selectivity and inverse agonism in S1P5 receptors.

The bioactive lysophospholipid sphingosine-1-phosphate (S1P) acts via five different subtypes of S1P receptors (S1PRs) - S1P1-5. S1P5 is predominantly expressed in nervous and immune systems, regulating the egress of natural killer cells from lymph nodes and playing a role in immune and neurodegenerative disorders, as well as carcinogenesis. Several S1PR therapeutic drugs have been developed to treat these diseases; however, they lack receptor subtype selectivity, which leads to side effects. In this article, we describe a 2.2 Å resolution room temperature crystal structure of the human S1P5 receptor in complex with a selective inverse agonist determined by serial femtosecond crystallography (SFX) at the Pohang Accelerator Laboratory X-Ray Free Electron Laser (PAL-XFEL) and analyze its structure-activity relationship data. The structure demonstrates a unique ligand-binding mode, involving an allosteric sub-pocket, which clarifies the receptor subtype selectivity and provides a template for structure-based drug design. Together with previously published S1PR structures in complex with antagonists and agonists, our structure with S1P5-inverse agonist sheds light on the activation mechanism and reveals structural determinants of the inverse agonism in the S1PR family.