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1.
PLoS Negl Trop Dis ; 11(12): e0006142, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-29287072

RESUMO

We investigated alternatives to whole blood for blood feeding of mosquitoes with a focus on improved stability and compatibility with mass rearing programs. In contrast to whole blood, an artificial blood diet of ATP-supplemented plasma was effective in maintaining mosquito populations and was compatible with storage for extended periods refrigerated, frozen, and as a lyophilized powder. The plasma ATP diet supported rearing of both Anopheles and Aedes mosquitoes. It was also effective in rearing Wolbachia-infected Aedes mosquitoes, suggesting compatibility with vector control efforts.


Assuntos
Trifosfato de Adenosina/farmacologia , Aedes/fisiologia , Anopheles/fisiologia , Insetos Vetores/fisiologia , Plasma/química , Wolbachia/fisiologia , Trifosfato de Adenosina/sangue , Aedes/efeitos dos fármacos , Aedes/microbiologia , Animais , Anopheles/efeitos dos fármacos , Anopheles/microbiologia , Substitutos Sanguíneos/química , Dieta , Suplementos Nutricionais , Feminino , Insetos Vetores/efeitos dos fármacos , Insetos Vetores/microbiologia , Masculino , Óvulo , Controle Biológico de Vetores , Reprodução/efeitos dos fármacos
2.
PLoS Negl Trop Dis ; 8(12): e3294, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25502564

RESUMO

BACKGROUND: The bacterial endosymbiont Wolbachia blocks the transmission of dengue virus by its vector mosquito Aedes aegypti, and is currently being evaluated for control of dengue outbreaks. Wolbachia induces cytoplasmic incompatibility (CI) that results in the developmental failure of offspring in the cross between Wolbachia-infected males and uninfected females. This increases the relative success of infected females in the population, thereby enhancing the spread of the beneficial bacterium. However, Wolbachia spread via CI will only be feasible if infected males are sufficiently competitive in obtaining a mate under field conditions. We tested the effect of Wolbachia on the competitiveness of A. aegypti males under semi-field conditions. METHODOLOGY/PRINCIPAL FINDINGS: In a series of experiments we exposed uninfected females to Wolbachia-infected and uninfected males simultaneously. We scored the competitiveness of infected males according to the proportion of females producing non-viable eggs due to incompatibility. We found that infected males were equally successful to uninfected males in securing a mate within experimental tents and semi-field cages. This was true for males infected by the benign wMel Wolbachia strain, but also for males infected by the virulent wMelPop (popcorn) strain. By manipulating male size we found that larger males had a higher success than smaller underfed males in the semi-field cages, regardless of their infection status. CONCLUSIONS/SIGNIFICANCE: The results indicate that Wolbachia infection does not reduce the competitiveness of A. aegypti males. Moreover, the body size effect suggests a potential advantage for lab-reared Wolbachia-males during a field release episode, due to their better nutrition and larger size. This may promote Wolbachia spread via CI in wild mosquito populations and underscores its potential use for disease control.


Assuntos
Aedes/microbiologia , Comportamento Competitivo/fisiologia , Vírus da Dengue/fisiologia , Wolbachia/fisiologia , Aedes/fisiologia , Aedes/virologia , Animais , Dengue/microbiologia , Dengue/prevenção & controle , Dengue/transmissão , Feminino , Masculino , Comportamento Sexual Animal/fisiologia
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