Identification of Nrf2 Activators from the Roots of Valeriana officinalis.

Various age-related chronic diseases have been linked to oxidative stress. The cellular antioxidant response pathway is regulated by the transcription factor nuclear erythroid factor 2. Therefore, plant-derived nuclear erythroid factor 2 activators might be useful therapeutics to stimulate the body's defense mechanisms. Our study focused on the discovery of potent nuclear erythroid factor 2 activators from medicinal plants. Initially, a variety of medicinal plant extracts were screened for nuclear erythroid factor 2 activity using a nuclear erythroid factor 2 luciferase reporter cell line. Among these, Valerian (Valeriana officinalis) root was identified as a potent candidate. Sequential extraction and bioassay-guided fractionation led to the isolation of four nuclear erythroid factor 2-active compounds, which were structurally identified by NMR and LC/HRMS as the known compounds isovaltrate, valtrate, jatamanvaltrate-P, and valerenic acid. These four compounds were then tested in relevant biological assays. Firstly, their effects on the expression of glutathione S-transferase, glutamate-cysteine ligase catalytic subunit, glutathione peroxidase, and heme oxygenase 1 were determined in HepG2 cells. Glutathione S-transferase P1 and glutamate-cysteine ligase catalytic subunit were upregulated by isovaltrate, valtrate, and jatamanvaltrate-P, while heme oxygenase 1 was upregulated by isovaltrate, jatamanvaltrate-P, and valerenic acid. The four compounds also increased the levels of glutathione and its metabolite, CysGly. As glutathione aids in the detoxification of hydrogen peroxide, cytoprotective effects of these four nuclear erythroid factor 2 activators against hydrogen peroxide toxicity were investigated, and indeed, the compounds significantly improved cell survival. This study provides evidence that four valepotriates from the roots of V. officinalis are activators of nuclear erythroid factor 2-mediated antioxidant and detoxification pathways. Our data might expand the medical use of this plant beyond its current application as a sleep aid.

Effect of Contacting Surface on the Performance of Thin-Film Force and Pressure Sensors.

Flexible force and pressure sensors are important for assessing the wear comfort of tightly fitting apparel. Their accuracy and repeatability depend on the sensor itself and the contacting surface. Measurements of the contact pressure on soft surfaces like human skin tend to be erroneous, which could be due to incorrect sensor calibrations. This study aims to examine the effects of human body parameters such as the hardness and temperature of the contacting surface by using a custom-made calibration setup and investigating the incorporation of rigid discs on the sensor surface. Two commercial force sensors, FlexiForce and SingleTact, and one pressure sensor, Pliance X, are used in the investigation. The findings reveal that adding rigid discs on both sides of the force sensors improves their sensitivity. Systematic calibration has been performed on the surfaces with different temperatures and hardness. The results show that FlexiForce and Pliance X tend to be affected by the changes in surface temperature and surface hardness. Prolonged testing time shows that the time dependence of SingleTact and Pliance X sensor is lower, which suggests that they are more suitable for lengthier evaluations in which interface pressure is exerted on the human body. In brief, sensor attachment and proper calibration should be thoroughly considered before using sensors for applications on soft surfaces, like the human body.