[Determination of the structure of the main component of an antibiotic complex produced by Streptomyces griseolus 182]. / Opredelenie struktury osnovnogo komponenta antibioticheskogo kompleksa, obrazuemogo Streptomyces griseolus 182.

In the programme for screening antibiotics with antifungal and immunosuppressing activity a culture of Streptomyces griseolus 182 was isolated. The culture produced a complex of oligomycin structure antibiotics. Individual components of the complex were isolated by HPLC. The complex was shown to contain three components at a ratio of 80:15:5. The findings were compared with the physico-chemical characteristics of the described antibiotics. On the basis of the analysis it was concluded that fraction 2 of the antibiotic complex 182 could be oligomycin A. A detailed comparative investigation of oligomycin A and component 2 with HPLC, FMR, IR spectroscopy and mass spectrometry revealed their identity. The other two components were shown to be oligomycins B and C.

[The anticonflict and antiamnestic actions of carnitine chloride]. / Antikonfliktnoe i antiamnesticheskoe deistvie karnitina khlorida.

Anticonflict and antiamnestic effects of original D,L-carnitine chloride have been studied. By using a model of electroshock amnesia, carnitine chloride showed a pronounced antiamnestic effects, by positively affecting the behaviour of animals exposed to a conflict situation. The neuropharmacological effects of carnitine chloride have been found to be implicated in the metabolism of proteins and lipids.

[Is ethanol a stressogenic factor for rats with a developed alcohol motivation?]. / Iavliaetsia li étanol stressogennym faktorom dlia krys so sformirovannoi alkogol'noi morivatsiei?

The male outbred rats, placed in common cages, demonstrated dose- and time-dependent increase of blood plasma ACTH level after handling and acute ethanol administration (1 and 4 g/kg). The pituitary response of isolated rats under condition of free choice between water and 15% ethanol solution was more pronounced after handling. Ethanol (4 g/kg) did not increase plasma blood level ACTH in isolated alcohol motivated rats. It also prevented blood plasma ACTH rise under condition of handling. The dual role of acute ethanol as a stressogenic and antistressogenic factor in naive and alcoholic rats is discussed.

[Positive modulation of diazepam activity by cortexolone in alcoholic rats]. / Pozitivnaia moduliatsiia aktivnosti diazepama u alkogolizirovannykh krys pri pomoshchi korteksolona.

It has been shown that glucocorticoid receptor antagonist-cortexolone--increased anxiolytic action of diazepam in alcoholic rats. Neither diazepam (2 mg/kg), nor cortexolone (20 mg/kg) alone influenced voluntary ethanol consumption in alcoholic rats during 14 days of administration, however, combined administration of diazepam and cortexolone diminished ethanol consumption. Receptor and permissive mechanism of gluco- and antiglucocorticoid effect on the action of diazepam are being discussed.

[Cortexolone as a modulator of diazepam activity]. / Korteksolon kak moduliator aktivnosti diazepama.

The influence of ACTH (200 micrograms/kg), corticosterone (20 mg/kg) and cortexolone (20 mg/kg) on the anxiolytic activity of diazepam was studied. ACTH partly and corticosterone completely blocked the action of diazepam. Cortexolone injection 30 min before the administration of diazepam induced a 100% anxiolytic effect of diazepam in the range of doses from 0.1 to 0.3 mg/kg (ED50 of anxiolytic diazepam effect is 0.2 mg/kg). The role of stress hormones in the regulation of psychotropic drug activity is discussed.

[Effect of compounds with anxiolytic properties on voluntary ethanol consumption by rats]. / Vliianie soedinenii s anksioliticheskimi svoistvami na dobrovol'noe potreblenie étanola u krys.

The effect of different chemical anxiolytic agents on ethanol consumption has been studied on the model of experimental alcoholism in rats. The decrease of ethanol consumption was dose-dependent. The existence of non-benzodiazepine anxiolytic systems is suggested.

[Role of benzodiazepine receptors in realizing the anxiolytic effect of compounds on intact rats and on animals with a physical dependence ethanol]. / Rol' benzodiazepinovykh retseptorov v realizatsii anksioliticheskogo éffekta soedinenii u intaktnykh krys i u zhivotnykh s fizicheskoi zavisimost'iu ot étanola.

Anxiolytic activity of DSIP, sodium hydroxybutyrate, nicotinoyl-GABA, mebicar, some derivatives of aminoandrostane and beta-carboline was not, like in the case of diazepam and beta C-3CEE, related to benzodiazepine receptors. The degree of the decrease in anxiolytic activity of these compounds did not correspond to increasing Ki binding of 3H diazepam in alcoholic rats.

[Psychotropic properties of hormonally inactive derivatives of androstane]. / Nekotorye psikhotropnye svoistva gormonal'no neaktivnykh proizvodnykh androstana.

It has been shown in experiments on noninbred male rats that 17 beta-acetylamino-5-androstene-3 beta,16 beta-diol, 17 beta-amino-5-androstene-3 beta,16 beta-diol hydrochloride, and 17 beta-acetylamino-4-androstene-3,16-dione have an anxiolytic action. These compounds do not exhibit any myorelaxant, anticonvulsant or antineurotic activity common to benzodiazepine tranquilizers. The anxiolytic effect of the compounds under consideration is coupled with the ability to reduce the level of alcoholic motivation in rats.

[Anxiolytic action of beta-carbolines in rats]. / Anksioliticheskaia aktivnost' nekotorykh beta-karbolinov u krys.

The decreased sensitivity to the anxiolytic action of diazepam, BC-3-KEE, IME-6MEO-TGBC, IME-6MEO-DBC was shown in rats with experimental alcoholism. The degree of the decreased sensitivity was dependent on the affinity of the compounds to benzodiazepine receptors.

[Prostaglandin F alpha and E content of the tissues of the snail Helix pomatia]. / Soderzhanie prostaglandinov F al'fa i E v tkaniakh ulitki Helix pomatia.

The content of prostaglandins of groups Fa and E has been determined radioimmunologically in the hemolymph, muscle tissue, nerves, ganglia and total preparation of the nervous system of the snail Helix pomatia. The highest prostaglandin content was found in the nervous ganglia, the lowest one - in the hemolymph and muscles. Concentration of prostaglandins E is by an order higher than that of prostaglandins Fa.