Biodegradation of monocrotophos, cypermethrin & fipronil by Proteus myxofaciens VITVJ1: A plant - microbe based remediation.

Current study was focused on the degradation of pesticides such as Monocrotophos, Cypermethrin & Fipronil (M, C & F) using phyto and rhizoremediation strategies. The isolate Proteus myxofaciens (VITVJ1) obtained from agricultural soil was capable of degrading M, C & F. The bacteria exhibited resistance to all the pesticides (M, C & F) up to 1500 ppm and was also capable of forming biofilms. The degraded products identified using Gas Chromatography-Mass Spectroscopy (GC-MS) and FTIR was further used for deriving the degradation pathway. The end product of M, C & F was acetic acid and 3-phenoxy benzoic acid which was confirmed by the presence of functional groups such as C=O and OH. Seed germination assay revealed the non-toxic nature of the degraded products with increased germination index in the treatments augmented with degraded products. The candidate genes such as opdA gene, Est gene and MnP1gene was amplified with the amplicon size of 700bp, 1200bp and 500bp respectively. P. myxofaciens not only degraded M, C & F, but was also found to be a plant growth promoting rhizobacteria. Since, it was capable of producing Indole Acetic acid (IAA), siderophore and was able to solubilize insoluble phosphate. Therefore, VITVJ1 upon augmentation to the rhizoremediation setup aided the degradation of pesticides with increase in plant growth as compared to that of the phytoremediation setup. To our knowledge this is the first study where P. myxofaciens has been effectively used for the degradation of three different classes of pesticides, which could also enhance the growth of plants and simultaneously degrade M, C & F.

Critical review on unveiling the toxic and recalcitrant effects of microplastics in aquatic ecosystems and their degradation by microbes.

Production of synthetic plastic obtained from fossil fuels are considered as a constantly growing problem and lack in the management of plastic waste has led to severe microplastic pollution in the aquatic ecosystem. Plastic particles less than 5mm are termed as microplastics (MPs), these are pervasive in water and soil, it can also withstand longer period of time with high durability. It can be broken down into smaller particles and can be adsorbed by various life-forms. Most marine organisms tend to consume plastic debris that can be accumulated easily into the vertebrates, invertebrates and planktonic entities. Often these plastic particles surpass the food chain, resulting in the damage of various organs and inhibiting the uptake of food due to the accumulation of microplastics. In this review, the physical and chemical properties of microplastics, as well as their effects on the environment and toxicity of their chemical constituents are discussed. In addition, the paper also sheds light on the potential of microorganisms such as bacteria, fungi, and algae which play a pivotal role in the process of microplastics degradation. The mechanism of microbial degradation, the factors that affect degradation, and the current advancements in genetic and metabolic engineering of microbes to promote degradation are also summarized. The paper also provides information on the bacterial, algal and fungal degradation mechanism including the possible enzymes involved in microplastic degradation. It also investigates the difficulties, limitations, and potential developments that may occur in the field of microbial microplastic degradation.

Deciphering the enhanced translocation of Pb, Ni and Cd from artificially polluted soil to Chrysopogon zizanioides augmented with Bacillus xiamenensis VITMSJ3.

The translocation of heavy metals (HMs) from the rhizosphere to plant systems constitutes a fundamental mechanism governing HM uptake. Microbial augmentation has emerged as a promising strategy to enhance this process. The study investigates the mechanism of enhanced translocation of heavy metals (HMs) from artificially polluted soil to Chrysopogon zizanioides, facilitated by Bacillus xiamenensis VITMSJ3. Pb, Ni, and Cd translocation to the roots and shoots of C. zizanioides was examined, revealing a significant increase of over 15% in HM uptake upon treatment with Bacillus xiamenensis VITMSJ3 (Accession number MT822866). VITMSJ3 exhibited biofilm formation capabilities, attributed to quorum sensing molecule production, and demonstrated resistance to Pb and Ni upto 4000 ppm and Cd upto 450 ppm, respectively. Moreover, VITMSJ3 displayed plant growth-promoting bacterial (PGPB) traits such as, indole-3-acetic acid (IAA), phosphate, ammonia, siderophore, and hydrogen cyanide (HCN) production. Amplification of candidate genes responsible for HM resistance (pbr for Pb, ncc for Ni, cadA for Cd) corroborated the genetic basis of resistance. SEM-EDAX micrographs confirmed HM uptake and translocation along with the presence of VITMSJ3. Enzymatic analysis revealed the synthesis of superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), peroxidase (POD), and ascorbate peroxidase (APX), implicating their involvement in ROS detoxification. Overall, the study underscores the efficacy of B. xiamenensis VITMSJ3 in enhancing HM translocation, thereby elucidating its potential for phytoremediation applications. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-024-04001-x.