RESUMO
INTRODUCTION: Capillary zone electrophoresis (CZE) at alkaline pH is one of the techniques used for hemoglobinopathy screening. In this study, an evaluation of the performance of a lower throughput CZE instrument, the Sebia Minicap Flex Piercing system, for this purpose is reported for the first time. METHODS: The analytical performance of the Sebia Minicap Flex Piercing system was evaluated. Furthermore, a method comparison between the Sebia Minicap Flex Piercing and two HPLC methods, that is, the Bio-Rad Variant Classic(™) and the Bio-Rad D-10(™) systems was performed by measuring samples with and without clinically relevant hemoglobin disorders. RESULTS: The analytical performance was acceptable for the determination of HbA, HbA2, HbS, and HbF, with an imprecision ≤2.0%. Method comparison showed a linear correlation for HbA2, HbF, and HbS measurements. Clinical concordance was acceptable when comparing CZE and HPLC. CONCLUSIONS: Lower throughput CZE using the Sebia Minicap Flex Piercing can be used for precise and accurate first line screening and follow-up of hemoglobinopathies.
Assuntos
Eletroforese Capilar/métodos , Hemoglobinopatias/diagnóstico , Cromatografia Líquida de Alta Pressão , Hemoglobina Fetal/química , Hemoglobina A2/química , Hemoglobina Falciforme/química , Hemoglobinopatias/sangue , Hemoglobinas/química , Humanos , Reprodutibilidade dos TestesRESUMO
Urinary schistosomiasis, caused by Schistosoma haematobium, is a prevalent parasitic infection in certain areas of Africa and the Middle East. Humans can be infected by cercariae when they are in contact with contaminated freshwater. The adult worms reside in the veins of the vesical and pelvis plexuses. The urinary bladder, the seminal vesicles and the lower ends of the ureters are the most commonly affected organs. In this case report, we describe an unrecognised case of urinary schistosomiasis in a woman who was part of a Belgian travel group; two other patients out of eight were also infected. In Belgium, the number of reported cases of S. haematobium infection is limited. The aim of this report is to emphasize this parasitic infection should be suspected in patients who travel to endemic areas.
Assuntos
Esquistossomose Urinária/diagnóstico , Viagem , Idoso , Anti-Helmínticos/uso terapêutico , Bélgica , Terapia Combinada , Diagnóstico Diferencial , Feminino , Água Doce , Humanos , Malaui , Masculino , Praziquantel/uso terapêutico , Esquistossomose Urinária/tratamento farmacológico , Esquistossomose Urinária/cirurgiaRESUMO
We report a unusual case of hydro-ureteronephrosis caused by schistosomiasis in a 66-year-old female. Computed tomography (CT) and biochemistry initially suggested a transitional cell carcinoma of the left proximal ureter. The patient was referred for reno-ureterectomy, but histopathological examination of the resection specimen demonstrated deposits of Schistosoma haematobium eggs. Although schistosomiasis is rare in Western Europe, this case illustrates the importance of considering infectious disease in patients with obstructive uropathy, particularly in the context of travelling or immigration from endemic areas.