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Methods Cell Biol ; 138: 221-238, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28129845

RESUMO

The lymphatic system is lined by endothelial cells and part of the vasculature. It is essential for tissue fluid homeostasis, absorption of dietary fats, and immune surveillance in vertebrates. Misregulation of lymphatic vessel formation and dysfunction of the lymphatic system have been indicated in a number of pathological conditions including lymphedema formation, obesity or chronic inflammatory diseases such as rheumatoid arthritis. In zebrafish, lymphatics were discovered about 10years ago, and the underlying molecular pathways involved in its development have since been studied in detail. Due to its superior live cell imaging possibilities and the broad tool kit for forward and reverse genetics, the zebrafish has become an important model organism to study the development of the lymphatic system during early embryonic development. In the current review, we will focus on the key players during zebrafish lymphangiogenesis and compare the roles of these genes to their mammalian counterparts. In particular, we will focus on novel findings that shed new light on the molecular mechanisms of lymphatic cell fate specification, as well as sprouting and migration of lymphatic precursor cells.


Assuntos
Células Endoteliais/ultraestrutura , Linfangiogênese , Vasos Linfáticos/diagnóstico por imagem , Imagem Molecular/métodos , Animais , Artrite Reumatoide/diagnóstico por imagem , Artrite Reumatoide/fisiopatologia , Modelos Animais de Doenças , Células Endoteliais/patologia , Humanos , Vasos Linfáticos/fisiopatologia , Linfedema/diagnóstico por imagem , Linfedema/fisiopatologia , Obesidade/diagnóstico por imagem , Obesidade/fisiopatologia , Peixe-Zebra/fisiologia
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