RESUMO
BACKGROUND: To evaluate for the first time in vivo the effects of methylene blue (MB) photosensitizer dissolved in ethanol in antimicrobial photodynamic therapy (aPDT) as adjuvant periodontal treatment, at plasmatic oxidative stress and vascular behavior in rat model. METHODS: Wistar rats were divided into negative control (NC, no periodontitis) and positive control (PC, with periodontitis, without any treatment). The other groups had periodontitis and were treated with scaling and root planing (SRP); SRP+aPDT+MB dissolved in water (aPDT I); SRP+aPDT+MB dissolved in ethanol (aPDT II). The periodontitis was induced by ligature at the mandibular right first molar. At 7/15/30days, rats were euthanized, the plasma was used to determine oxidative stress parameters and gingival tissue for histomorphometric analysis. RESULTS: PC showed higher thiobarbituric acid reactive substances levels in 7/15/30days. aPDT II was able to block the lipid peroxidation, especially between 15th and 30th days. Glutathione reduced levels were consumed in PC, aPDT I and II groups throughout the experiment. aPDT II increased the vitamin C levels which were restored in this group in the 30th day. aPDT II group showed the highest number of blood vessels. CONCLUSION: In summary, the aPDT with MB dissolved in ethanol provides better therapeutic responses in periodontitis treatment.
Assuntos
Anti-Infecciosos/farmacologia , Azul de Metileno/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Fármacos Fotossensibilizantes/farmacologia , Animais , Anti-Infecciosos/uso terapêutico , Ácido Ascórbico/sangue , Vasos Sanguíneos/efeitos dos fármacos , Vasos Sanguíneos/patologia , Vasos Sanguíneos/efeitos da radiação , Raspagem Dentária , Gengiva/patologia , Gengiva/efeitos da radiação , Glutationa/sangue , Luz , Masculino , Azul de Metileno/uso terapêutico , Estresse Oxidativo/efeitos da radiação , Periodontite/tratamento farmacológico , Periodontite/radioterapia , Fotoquimioterapia , Fármacos Fotossensibilizantes/uso terapêutico , Ratos , Ratos Wistar , Aplainamento RadicularRESUMO
This article presents the evolution and development that the Programmatic Area of Dissemination of Scientific and Technical Information of INCAP has had during the last five years. It therefore includes, objectives, activities conducted, and a description of achieved products, as well as short and medium-term perspectives foreseen.